Effect of heat inactivation on the detection of the RNA of SARS-CoV-2 by RT-qPCR
Chinese Journal of Virology
; 36(6):1004-1008, 2020.
Article
Dans Chinois
| GIM | ID: covidwho-2034524
ABSTRACT
SARS-CoV-Z treated at 56 degrees C for 30 min can be inactivated effectively. However, the effect of heat treatment on subsequent detection of the RNA of SARS-CoV-2 by real-time reverse transcription-quantitative polymerase Chain reaction (RT-qPCR) has not been reported. We filled this knowledge gap in present study. We used five SARS'CoV-Z-positive throat swabs. Each throat swab was divided into four parts and assigned to a group control;56 degrees C for 30 min;56 degrees C for 45 min;56 degrees C for 60 min. After heat treatment, SARS-CoV-Z RNA was extracted and detected by RT-qPCR (absolute quantitation using a standard curve). We found that SARS- CoV-Z RNA was reduced by ~40% after treatment at 56 degrees C for 30 ' 60 min. There was no significant difference (P > 0.05 for all) in the test results between the treatment groups (56 degrees C for 30 min;56 degrees C for 45 min;56 degrees C for 60 min). Our study suggested that SARS-CoV-Z specimens could be inactivated at 56 degrees C for 30 min, before RNA extraction and RT-qPCR detection, which could protect the safety of personnel and the environment during testing. Heat inactivation had a limited effect upon RT-qPCR detection but it should be used with caution if the specimen result is near the critical value.
63231-63-0; confirmatory tests; coronavirus disease 2019; detection; diagnostic techniques; duration; extraction; heat treatment; human diseases; protein extraction; Rna; throat; time; man; Severe acute respiratory syndrome coronavirus 2; viruses; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; heat processing; ribonucleic acid; SARS-CoV-2
Collection:
Bases de données des oragnisations internationales
Base de données:
GIM
Type d'étude:
Études expérimentales
langue:
Chinois
Revue:
Chinese Journal of Virology
Année:
2020
Type de document:
Article
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