Synthesis and immunogenicity assessment of a gold nanoparticle conjugate for the delivery of a peptide from SARS-CoV-2.
Nanomedicine
; 34: 102372, 2021 06.
Artigo
em Inglês
| MEDLINE | ID: covidwho-1117330
ABSTRACT
The development of vaccines is a crucial response against the COVID-19 pandemic and innovative nanovaccines could increase the potential to address this remarkable challenge. In the present study a B cell epitope (S461-493) from the spike protein of SARS-CoV-2 was selected and its immunogenicity validated in sheep. This synthetic peptide was coupled to gold nanoparticles (AuNP) functionalized with SH-PEG-NH2 via glutaraldehyde-mediated coupling to obtain the AuNP-S461-493 candidate, which showed in s.c.-immunized mice a superior immunogenicity (IgG responses) when compared to soluble S461-493; and led to increased expression of relevant cytokines in splenocyte cultures. Interestingly, the response triggered by AuNP-S461-493 was similar in magnitude to that induced using a conventional strong adjuvant (Freund's adjuvant). This study provides a platform for the development of AuNP-based nanovaccines targeting specific SARS-CoV-2 epitopes.
Palavras-chave
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Epitopos de Linfócito B
/
Nanopartículas Metálicas
/
Glicoproteína da Espícula de Coronavírus
/
Imunogenicidade da Vacina
/
Vacinas contra COVID-19
/
Ouro
Tipo de estudo:
Estudo prognóstico
Tópicos:
Vacinas
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Nanomedicine
Assunto da revista:
Biotecnologia
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
J.nano.2021.102372
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