Low-level laser therapy for treatment of severe acute respiratory syndrome 2 infection
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
; 12377, 2023.
Artigo
em Inglês
| Scopus | ID: covidwho-20239867
ABSTRACT
SARS-CoV-2 is a new threat to public health due to its increased transmissibility and immune evasion. Angiotensin-converting enzyme 2 (ACE2) plays a critical role in SARS-CoV-2 infection as its serve as the virus's major entry receptor in humans. Vaccines have been authorized for emergency use to control the current pandemic and they have greatly reduced the spread of SARS-CoV-2 and mortality rates, nevertheless this coronavirus has shown the ability to endure crucial mutations that increases its infectivity which makes it likely that the virus will continue to mutate and disseminate. There is a need to find and introduce alternative and effective methods of controlling SARS-CoV-2. Notably, low-level laser therapy (LLLT) is a method of exposing cells or tissue to low levels of red and near infrared light which has a high success rate for treatment of other ailments. The aim of the study is to determine for the first time, the effects of LLLT on SARS-CoV-2 infected HEK293/ACE2 cells and compare them to uninfected ones. Both infected and uninfected HEK293/ACE2 cells were irradiated at a wavelength of 640 nm, at different doses. Then, the effects of laser irradiation on the cells and the virus were evaluated using luciferase, cytotoxicity, and cell viability assays. Preliminary results showed that irradiated uninfected cells had no changes in cell viability and cytotoxicity, while there were changes in irradiated infected cells. In addition, laser irradiation caused cell membrane damage in infected cells. Lastly, uninfected irradiated cells showed no luciferase activity while laser irradiation reduced luciferase activity in infected cells. © 2023 SPIE.
fluences; HEK293/ACE2 cells; infected; Low level lase therapy; SARS-CoV-2; uninfected; Cells; Cytology; Diseases; Enzyme activity; Health risks; Infrared devices; Infrared lasers; Irradiation; Laser surgery; Laser tissue interaction; Angiotensin-converting enzyme 2; HEK-293; Hek293/angiotensin-converting enzyme 2 cell; Infected cells; Low level laser therapy; Luciferase activities; Coronavirus
Texto completo:
Disponível
Coleções:
Bases de dados de organismos internacionais
Base de dados:
Scopus
Tipo de estudo:
Estudo experimental
Tópicos:
Vacinas
Idioma:
Inglês
Revista:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Ano de publicação:
2023
Tipo de documento:
Artigo
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