[Component identification and analysis in vivo of Sanhan Huashi formula].
Zhongguo Zhong Yao Za Zhi
; 48(8): 2126-2143, 2023 Apr.
Artigo
em Chinês
| MEDLINE | ID: covidwho-20245305
ABSTRACT
Sanhan Huashi formula(SHF) is the intermediate of a newly approved traditional Chinese medicine(TCM) Sanhan Huashi Granules for the treatment of COVID-19 infection. The chemical composition of SHF is complex since it contains 20 single herbal medicines. In this study, UHPLC-Orbitrap Exploris 240 was used to identify the chemical components in SHF and in rat plasma, lung and feces after oral administration of SHF, and heat map was plotted for characterizing the distribution of the chemical components. Chromatographic separation was conducted on a Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm, 1.7 µm) using 0.1% formic acid(A)-acetonitrile(B) as mobile phases in a gradient elution. Electrospray ionization(ESI) source was used to acquire data in positive and negative mode. By reference to quasi-molecular ions and MS/MS fragment ions and in combination with MS spectra of reference substances and compound information in literature reports, 80 components were identified in SHF, including 14 flavonoids, 13 coumarins, 5 lignans, 12 amino-compounds, 6 terpenes and 30 other compounds; 40 chemical components were identified in rat plasma, 27 in lung and 56 in feces. Component identification and characterization of SHF in vitro and in vivo lay foundations for disclosure of its pharmacodynamic substances and elucidation of the scientific connotation.
Palavras-chave
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Lignanas
/
COVID-19
Tipo de estudo:
Estudo diagnóstico
Tópicos:
Medicina tradicional
Limite:
Animais
Idioma:
Chinês
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
Farmacologia
/
Terapias Complementares
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
J.cnki.cjcmm.20230203.301
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