Evaluation of two different domestic colloidal gold assay kit fornew coronavirus antibody in low endemic areas
Journal of Modern Laboratory Medicine
; 36(3):103-107, 2021.
Artigo
em Chinês
| GIM | ID: covidwho-2055550
ABSTRACT
This study aimed to evaluate the application and diagnostic efficacy of two different colloidal gold kits for the detection of 2019-nCoV immunoglobulin M antibody (anti-IgM) and immunoglobulin G antibody (anti-IgG) in Beijing, a low endemic area, and to guide the rational clinical application. The sera of 29 patients with confirmed novel coronavirus pneumonia (COVID-19) and 19 411 patients from the non-infected screening population were selected to evaluate the sensitivity, specificity and false-positive rate of the 2019-nCoV antibody test kits from Zhuhai Lizhu and Tangshan Innotek using colloidal gold immunochromatography. The sensitivity of Inotec 2019-nCoV was slightly higher than that of Lizhu 2019-nCoV, with a sensitivity of 58.62% and 55.17%, respectively;the specimen collection time of the all-negative group was significantly less than that of the antibody-positive group (P < 0.05);the false-positive rate of the two reagents in the low-prevalence area was 0.16%, and the false-positive rate of 2019-nCoV IgG was higher in Inotec than in Lizhu. The false positive rate for 2019-nCoV IgM was significantly higher than that for IgG for the same brand (Inotec ?2=14.756 09, P=0.000 0;Lizhu ?2=27.492 62, P=0.000). Conclusion The 2019-nCoV antibody test is rapid, simple and easy to perform, with high specificity, and can be used as a rapid screening indicator for new crowns;the specificity, correctness and negative predictive value of the two kits are good, and the application of the other kit for retesting when a positive result occurs can reduce the false positive rate of informing the clinic;the application and analysis of positive reports of new crown antibodies should be combined with the endemic area and clinical comprehensive judgment.
7440-57-5; 308067-58-5; antibodies; antibody testing; colloids; comparisons; coronavirus disease 2019; diagnosis; diagnostic techniques; false positive results; gold; human diseases; IgG; immunological techniques; negative predictive value; patients; pneumonia; rapid methods; screening; viral diseases; man; Severe acute respiratory syndrome coronavirus 2; Beijing; China; Northern China; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; antibody detection; antibody tests; Peking; People's Republic of China; serological techniques; screening tests; SARS-CoV-2; viral infections
Texto completo:
Disponível
Coleções:
Bases de dados de organismos internacionais
Base de dados:
GIM
Tipo de estudo:
Estudo experimental
Idioma:
Chinês
Revista:
Journal of Modern Laboratory Medicine
Ano de publicação:
2021
Tipo de documento:
Artigo
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