Visual detection and differentiation of porcine epidemic diarrhea virus wild-type strains and attenuated vaccine strains using CRISPR/Cas13a-based lateral flow strip.
Front Cell Infect Microbiol
; 12: 976137, 2022.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2109734
ABSTRACT
Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that causes acute watery diarrhea and vomiting in unweaned piglets. Infections result in high mortality and serious economic losses to the swine industry. PEDV attenuated vaccine does not completely protect against all mutant wild-type strains, and PEDV infection can periodically occur. A sensitive, accurate, and simple detection method for PEDV is needed to reduce the occurrence of the disease. In this study, the CRISPR/Cas13a system was combined with recombinase aided amplification to develop a rapid diagnostic method to distinguish PEDV wild-type strains from attenuated vaccine strains. The method is based on isothermal detection at 37°C. The results are used for visual readout. The assay had high sensitivity and specificity, with a detection limit of 101 copies/µL for the gene of interest, and no cross-reactivity with other pathogens. The Cas13a detection worked well with clinical samples. This visual, sensitive, and specific nucleic acid detection method based on CRISPR/Cas13a should be a powerful tool for detecting PEDV.
Palavras-chave
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Ácidos Nucleicos
/
Infecções por Coronavirus
/
Vírus da Diarreia Epidêmica Suína
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Ensaios controlados aleatorizados
Tópicos:
Vacinas
Limite:
Animais
Idioma:
Inglês
Revista:
Front Cell Infect Microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
País de afiliação:
Fcimb.2022.976137
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