Advances in droplet digital polymerase chain reaction on microfluidic chips.
Lab Chip
; 23(5): 1258-1278, 2023 03 01.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2237144
ABSTRACT
The PCR technique has been known to the general public since the pandemic outbreak of COVID-19. This technique has progressed through three stages from simple PCR to real-time fluorescence PCR to digital PCR. Among them, the microfluidic-based droplet digital PCR technique has attracted much attention and has been widely applied due to its advantages of high throughput, high sensitivity, low reagent consumption, low cross-contamination, and absolute quantification ability. In this review, we introduce various designs of microfluidic-based ddPCR developed within the last decade. The microfluidic-based droplet generation methods, thermal cycle strategies, and signal counting approaches are described, and the applications in the fields of single-cell analysis, disease diagnosis, and pathogen detection are introduced. Further, the challenges and prospects of microfluidic-based ddPCR are discussed. We hope that this review can contribute to the further development of the microfluidic-based ddPCR technique.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Microfluídica
/
COVID-19
Tipo de estudo:
Estudo diagnóstico
/
Ensaios controlados aleatorizados
Limite:
Humanos
Idioma:
Inglês
Revista:
Lab Chip
Assunto da revista:
Biotecnologia
/
Química
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
D2lc00814a
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