A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry.
Cell
; 186(4): 850-863.e16, 2023 02 16.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2239711
ABSTRACT
It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence.
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Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Infecções por Coronavirus
/
Coronavirus
/
Dipeptidil Peptidase 4
/
Pangolins
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Cell
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
J.cell.2023.01.019
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