Genetic and enzymatic characterization of two novel blaNDM-36, -37 variants in Escherichia coli strains.
Eur J Clin Microbiol Infect Dis
; 42(4): 471-480, 2023 Apr.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2270115
ABSTRACT
The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel blaNDM variants of blaNDM-36, -37 were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the blaNDM-36, -37 enzymes and their carrying strains. The blaNDM-36, -37 harboring E. coli isolates belonged to ST227, O9H10 serotype and exhibited intermediate or resistance to all ß-lactams tested except aztreonam and aztreonam/avibactam. The genes of blaNDM-36, -37 were located on a conjugative IncHI2-type plasmid. NDM-37 differed from NDM-5 by a single amino acid substitution (His261Tyr). NDM-36 differed from NDM-37 by an additional missense mutation (Ala233Val). NDM-36 had increased hydrolytic activity toward ampicillin and cefotaxime relative to NDM-37 and NDM-5, while NDM-37 and NDM-36 had lower catalytic activity toward imipenem but higher activity against meropenem in comparison to NDM-5. This is the first report of co-occurrence of two novel blaNDM variants in E. coli isolated from the same patient. The work provides insights into the enzymatic function and demonstrates the ongoing evolution of NDM enzymes.
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Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
/
Infecções por Escherichia coli
Tipo de estudo:
Estudo prognóstico
Tópicos:
Variantes
Limite:
Humanos
Idioma:
Inglês
Revista:
Eur J Clin Microbiol Infect Dis
Assunto da revista:
Doenças Transmissíveis
/
Microbiologia
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
S10096-023-04576-y
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