Portable Paper-Based Nucleic Acid Enrichment for Field Testing.
Adv Sci (Weinh)
; 10(11): e2205217, 2023 04.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2271765
ABSTRACT
Point-of-care testing (POCT) can be the method of choice for detecting infectious pathogens; these pathogens are responsible for not only infectious diseases such as COVID-19, but also for certain types of cancers. For example, infections by human papillomavirus (HPV) or Helicobacter pylori (H. pylori) are the main cause of cervical and stomach cancers, respectively. COVID-19 and many cancers are treatable with early diagnoses using POCT. A variety of nucleic acid testing have been developed for use in resource-limited environments. However, questions like unintegrated nucleic acid extraction, open detection systems increase the risk of cross-contamination, and dependence on expensive equipment and alternating current (AC) power supply, significantly limit the application of POCT, especially for on-site testing. In this paper, a simple portable platform is reported capable of rapid sample-to-answer testing within 30 min based on recombinase polymerase amplification (RPA) at a lower temperature, to detect SARS-CoV-2 virus and H. pylori bacteria with a limit of detection as low as 4 × 102 copies mL-1 . The platform used a battery-powered portable reader for on-chip one-pot amplification and fluorescence detection, and can test for multiple (up to four) infectious pathogens simultaneously. This platform can provide an alternative method for fast and reliable on-site diagnostic testing.
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Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Doenças Transmissíveis
/
COVID-19
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Ensaios controlados aleatorizados
Limite:
Humanos
Idioma:
Inglês
Revista:
Adv Sci (Weinh)
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
Advs.202205217
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