A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening.
Biosensors (Basel)
; 11(10)2021 Oct 02.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2278217
ABSTRACT
The COVID-19 pandemic has changed people's lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society's burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory. To this end, we developed a portable and semi-automated device for SARS-CoV-2 detection based on reverse transcription loop-mediated isothermal amplification followed by a CRISPR/Cas12a reaction. The device contains a heater element mounted on a printed circuit board, a cooler fan, a proportional integral derivative controller to control the temperature, and designated areas for 0.2 mL Eppendorf® PCR tubes. Our system has a limit of detection of 35 copies of the virus per microliter, which is significant and has the capability of being used in crisis centers, mobile laboratories, remote locations, or airports to diagnose individuals infected with SARS-CoV-2. We believe the current methodology that we have implemented in this article is beneficial for the early screening of infectious diseases, in which fast screening with high accuracy is necessary.
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Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Amplificação de Ácido Nucleico
/
Técnicas de Diagnóstico Molecular
/
Sistemas CRISPR-Cas
/
SARS-CoV-2
/
COVID-19
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
Bios11100369
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