Effectiveness of rapid immunochromatographic tests to the detection of feline Coronavirus
Veterinrstv
; 72(10), 2022.
Artigo
em Tcheco
| CAB Abstracts | ID: covidwho-2285711
ABSTRACT
Feline coronavirus (FCoV) belongs among pathogens with common occurrence in the cats population in the whole world. FCoV is ubiquitous in environments with a higher concentration of cats, e.g. in shelters, multicat households and kennels. FCoV primarily attacks the digestive feline tract, replicates in its cells and is excreted in the feces to surroundings of permanently or transiently infected cats. The aim of the study was the detection of FCoV in the feces of newly admitted cats to the shelter by the qPCR method and by means of commercial rapid immunochromatographic (antigen) tests from three different producers. For each of the antigen tests, sensitivity and specifity were determined by comparison with the qPCR analysis result. Out of 70 examined fecal samples, viral RNA was by the qPCR analysis identified in 44 samples (62.9%). Neither the age nor the gender of cats played a significant role in the viral excretion. Found sensitivity of the antigen tests was at a low (< 35%;tests A and C) to a satisfactory level (> 50%, test B). The number of viral particles in the samples determined by the qPCR method did not correlate significantly with the result of the antigen tests. The results of this study suggest that the use of rapid antigen tests for routine screening of FCoV shedding in feline shelters is limited due to the high rate of false-negative results.
Pets and Companion Animals [LL070], Prion; Viral; Bacterial and Fungal Pathogens of Animals [LL821], Diagnosis of Animal Diseases [LL886], Diagnostic; Therapeutic and Pharmacological Biotechnology [WW700], Techniques and Methodology [ZZ900], chromatography, diagnosis, diagnostic techniques, faecal examination, false negative results, immunodiagnosis, polymerase chain reaction, rapid methods, RNA, shelters, viral antigens, cats, Feline coronavirus, Czech Republic, Felis, Felidae, Fissipeda, carnivores, mammals, vertebrates, Chordata, animals, eukaryotes, Central Europe, Europe, European Union Countries, high income countries, OECD Countries, very high Human Development Index countries, Alphacoronavirus 1, Alphacoronavirus, Coronavirinae, Coronaviridae, Nidovirales, positive-sense ssRNA Viruses, ssRNA Viruses, RNA Viruses, viruses, fecal examination, serological diagnosis, PCR, ribonucleic acid
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Bases de dados de organismos internacionais
Base de dados:
CAB Abstracts
Tipo de estudo:
Estudo experimental
Idioma:
Tcheco
Revista:
Veterinrstv
Ano de publicação:
2022
Tipo de documento:
Artigo
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