<p><b>OBJECTIVE</b>To investigate the distribution of
HPV 16 variants in Han
women patients without
Cervical intraepithelial neoplasia in the
diagnosis and
treatment center for cervical
disease, department of
Obstetrics and
Gynecology in
China-
Japan friendship hospital with
HPV 16 E5 sequence
phylogenetic analysis.</p><p><b>
METHODS</b>
PCR amplification of
HPV 16 E5 sequences and sequenced. The
association between variations types and different cervical lesions was analyzed.</p><p><b>RESULTS</b>In this
research, We first found that variant
classification based on
HPV 16 E5
DNA sequence (236 bp) alone had high rate of accuracy. In addition, for the first
time, our
research revealed that single-4075T can distinguished the As variant from all other variants.</p><p><b>CONCLUSION</b>If E5 sequence was used for
phylogenetic analysis, it
will greatly reduce the experimental
costs and improve
efficiency and
cost-
effectiveness. For the first
time, our
research revealed that single 4075T can distinguished the As variant from all other variants.</p>