Identification and verification of key salt-tolerant amino acid sites of banana MaNHX5 / 生物工程学报
Ya XU; Mengxin TENG; Fei QIAO; Xingyu JIANG; Yang ZHOU; Xinguo LI.
Chinese Journal of Biotechnology
; (12): 1946-1952, 2022.
Artículo
en Zh
| WPRIM | ID: wpr-927829
In order to improve the salt tolerance of banana NHX genes, we cloned a MaNHX5 gene from Musa acuminata L. AAA group and predicted the key salt-tolerant amino acid sites and mutant protein structure changes of MaNHX5 by using bioinformatics tools. The 276-position serine (S) of MaNHX5 protein was successfully mutated to aspartic acid (D) by site-directed mutagenesis, and the AXT3 salt-sensitive mutant yeast was used for a functional complementation test. The results showed that after the mutated MaNHX5 gene was transferred to AXT3 salt-sensitive mutant yeast, the salt tolerance of the mutant yeast was significantly improved under 200 mmol/L NaCl treatment. It is hypothesized that Ser276 of MaNHX5 protein plays an important role in the transport of Na+ across the tonoplast.
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