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1.
PLoS One ; 4(11): e7892, 2009 Nov 19.
Article in English | MEDLINE | ID: mdl-19936257

ABSTRACT

BACKGROUND: We report the detailed development of biomarkers to predict the clinical outcome under dengue infection. Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome gene-expression microarray data, validated by quantitative PCR and tested in independent samples. METHODOLOGY/PRINCIPAL FINDINGS: The study was performed on patients of a well-characterized dengue cohort from Recife, Brazil. The samples analyzed were collected prospectively from acute febrile dengue patients who evolved with different degrees of disease severity: classic dengue fever or dengue hemorrhagic fever (DHF) samples were compared with similar samples from other non-dengue febrile illnesses. The DHF samples were collected 2-3 days before the presentation of the plasma leakage symptoms. Differentially-expressed genes were selected by univariate statistical tests as well as multivariate classification techniques. The results showed that at early stages of dengue infection, the genes involved in effector mechanisms of innate immune response presented a weaker activation on patients who later developed hemorrhagic fever, whereas the genes involved in apoptosis were expressed in higher levels. CONCLUSIONS/SIGNIFICANCE: Some of the gene expression signatures displayed estimated accuracy rates of more than 95%, indicating that expression profiling with these signatures may provide a useful means of DHF prognosis at early stages of infection.


Subject(s)
Dengue/diagnosis , Dengue/genetics , Fever/diagnosis , Gene Expression Regulation , Adult , Aged , Aged, 80 and over , Apoptosis , Biomarkers/metabolism , Brazil , Cohort Studies , Dengue/metabolism , Dengue Virus/genetics , Dengue Virus/metabolism , Female , Fever/metabolism , Humans , Male , Middle Aged , Multivariate Analysis , Oligonucleotide Array Sequence Analysis , Treatment Outcome
2.
PLoS One ; 4(10): e7425, 2009 Oct 13.
Article in English | MEDLINE | ID: mdl-19826631

ABSTRACT

BACKGROUND: Dengue virus infection is a growing global public health concern in tropical and subtropical regions of the world. Dengue vaccine development has been hampered by concerns that cross-reactive immunological memory elicited by a candidate vaccine could increase the risk of development of more severe clinical forms. One possible strategy to reduce risks associated with a dengue vaccine is the development of a vaccine composed of selected critical epitopes of each of the serotypes. METHODOLOGY/PRINCIPAL FINDINGS: Synthetic peptides were used to identify B-cell epitopes in the envelope (E) glycoprotein of dengue virus type 3 (DENV-3). Eleven linear, immunodominant epitopes distributed in five regions at amino acid (aa) positions: 51-65, 71-90, 131-170, 196-210 and 246-260 were identified by employing an enzyme- linked immunosorbent assay (ELISA), using a pool of human sera from dengue type 3 infected individuals. Peptides 11 (aa51-65), 27 and 28 (aa131-150) also reacted with dengue 1 (DENV-1) and dengue 2 (DENV-2) patient sera as analyzed through the ROC curves generated for each peptide by ELISA and might have serotype specific diagnostic potential. Mice immunized against each one of the five immunogenic regions showed epitopes 51-65, 131-170, 196-210 and 246-260 elicited the highest antibody response and epitopes131-170, 196-210 and 246-260, elicited IFN-gamma production and T CD4+ cell response, as evaluated by ELISA and ELISPOT assays respectively. CONCLUSIONS/SIGNIFICANCE: Our study identified several useful immunodominant IgG-specific epitopes on the envelope of DENV-3. They are important tools for understanding the mechanisms involved in antibody dependent enhancement and immunity. If proven protective and safe, in conjunction with others well-documented epitopes, they might be included into a candidate epitope-based vaccine.


Subject(s)
Dengue Virus/chemistry , Dengue/immunology , Dengue/virology , Epitopes, B-Lymphocyte/chemistry , Viral Envelope Proteins/chemistry , Animals , Dengue Vaccines , Enzyme-Linked Immunosorbent Assay , Epitopes/chemistry , Humans , Immunoglobulin G/chemistry , Immunologic Memory , Mice , Mice, Inbred BALB C , Molecular Conformation , ROC Curve
3.
Trop Med Int Health ; 14(10): 1278-86, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19708899

ABSTRACT

OBJECTIVES: To identify the aetiological agents of cutaneous leishmaniasis and to investigate the genetic polymorphism of Leishmania (Viannia) parasites circulating in an area with endemic cutaneous leishmaniasis (CL) in the Atlantic rainforest region of northeastern Brazil. METHODS: Leishmania spp. isolates came from three sources: (i) patients diagnosed clinically and parasitologically with CL based on primary lesions, secondary lesions, clinical recidiva, mucocutaneous leishmaniasis and scars; (ii) sentinel hamsters, sylvatic or synanthropic small rodents; and (iii) the sand fly species Lutzomyia whitmani. Isolates were characterised using monoclonal antibodies, multilocus enzyme electrophoresis (MLEE) and polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region rDNA locus. RESULTS: Seventy-seven isolates were obtained and characterised. All isolates were identified as Leishmania (Viannia) braziliensis serodeme 1 based on reactivity to monoclonal antibodies. MLEE identified 10 zymodemes circulating in the study region. Most isolates were classified as zymodemes closely related to L. (V.) braziliensis, but five isolates were classified as Leishmania (Viannia) shawi. All but three of the identified zymodemes have so far been observed only in the study region. Enzootic transmission and multiclonal infection were observed. CONCLUSIONS: Our results confirm that transmission cycle complexity and the co-existence of two or more species in the same area can affect the level of genetic polymorphism in a natural Leishmania population. Although it is not possible to make inferences as to the modes of genetic exchange, one can speculate that some of the zymodemes specific to the region are hybrids of L. (V.) braziliensis and L. (V.) shawi.


Subject(s)
Genes, rRNA/genetics , Leishmania braziliensis/genetics , Leishmaniasis, Cutaneous/parasitology , Animals , Brazil/epidemiology , Disease Reservoirs , Humans , Leishmania braziliensis/classification , Leishmania braziliensis/isolation & purification , Leishmaniasis, Cutaneous/epidemiology , Molecular Epidemiology/methods , Sequence Analysis, DNA , Species Specificity
4.
Vet J ; 182(2): 356-8, 2009 Nov.
Article in English | MEDLINE | ID: mdl-18603455

ABSTRACT

Recent research has demonstrated the high sensitivity of real time PCR (qPCR) in the diagnosis of Leishmania infantum infection. The goal of this study was to develop and evaluate a qPCR detection system for the diagnosis of visceral leishmaniosis (VL) in dogs. Specific primer sets were developed for the Leishmania donovani complex, in which a fragment of 132 bp of kDNA from L. infantum was amplified. The reaction was performed using the ABI PRISM 7000 system with ABI PRISM software used to carry out the analysis. When canine blood samples were assessed using this system the detection limit of the method was found to be 0.07 parasites per reaction, the efficiency was 94.17% (R(2)=0.93, slope=-3.47) and the sensitivity and specificity were 100% and 83.33% respectively. The use of such a sensitive, reproducible and rapid qPCR-based assay will be useful in the diagnosis and control of L. infantum infection in endemic areas, where serological surveys often underestimate true disease prevalence.


Subject(s)
DNA, Kinetoplast/blood , Dog Diseases/parasitology , Leishmania infantum/genetics , Leishmania infantum/isolation & purification , Leishmaniasis, Visceral/veterinary , Polymerase Chain Reaction/veterinary , Animals , DNA, Kinetoplast/genetics , Dog Diseases/diagnosis , Dogs , Leishmaniasis, Visceral/blood , Leishmaniasis, Visceral/parasitology , Polymerase Chain Reaction/methods , Reproducibility of Results , Sensitivity and Specificity
5.
Acta Trop ; 108(2-3): 229-33, 2008.
Article in English | MEDLINE | ID: mdl-18851939

ABSTRACT

Specific immunoglobulin (IgA, IgG and IgM) responses to different antigen targets (soluble eggs antigen--SEA, soluble worm adult protein--SWAP and keyhole limpet hole--KLH) were measured by enzyme linked immunosorbent assay (ELISA) in patients with acute and chronic schistosomiasis, as well as patients without schistosomiasis. SEA IgA and KLH IgM presented high discriminatory powers to distinguish acute from chronic schistosomiasis, with calculated areas under the curve (AUCs) of 0.88 and 0.82, respectively, obtained from receiver operating characteristic (ROC) curve. On the other hand, these tests, particularly SEA IgA were not useful to distinguish schistosomiasis (including the acute and chronic forms) from individuals without this disease, but infected with other intestinal parasites (Ascaris lumbricoides, Trichuris trichiura and hookworm). By contrast, SWAP IgG and SEA IgG were able to discriminate schistosomiasis patients from healthy individuals and patients infected with other parasites (AUCs of 0.96 and 0.85, respectively). Thus, it is possible to use a combination of serological tests, such as SEA IgA and SWAP IgG, to simultaneously establish the diagnosis of schistosomiasis and discriminate the acute from the chronic forms of the disease.


Subject(s)
Antibodies, Helminth/blood , Schistosomiasis mansoni/diagnosis , Adolescent , Adult , Aged , Animals , Antigens, Helminth , Area Under Curve , Child , Child, Preschool , Diagnosis, Differential , Enzyme-Linked Immunosorbent Assay/methods , Female , Humans , Immunoglobulin A/blood , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged , ROC Curve
6.
Mem Inst Oswaldo Cruz ; 103(4): 401-4, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18660997

ABSTRACT

The aim of this work was to study the difference in interferon gamma (IFN-gamma) production by T lymphocytes after early secretory antigen target 6 (ESAT-6) or purified protein derivate (PPD) stimulation in whole blood culture supernatants from children with suspected tuberculosis (TB) disease (n = 21), latent TB infection (n = 16) and negative controls (NC) (n = 22) from an endemic area in Brazil. The concentration of IFN-gamma (pg/ml) was measured by enzyme linked immunosorbent assay and the differences in the IFN-gamma levels for each group were compared and evaluated using an unpaired Student's t-test; p values < 0.05 were considered significant. Measurement of IFN-gamma levels after ESAT-6 stimulation raised the possibility of early diagnosis in the latent TB group (p = 0.0030). Nevertheless, the same group showed similar responses to the NC group (p > 0.05) after PPD stimulation. The IFN-gamma assay using ESAT-6 as an antigenic stimulus has the potential to be used as a tool for the immunodiagnosis of early TB in children.


Subject(s)
Antigens, Bacterial , Bacterial Proteins , Interferon-gamma/biosynthesis , Mycobacterium tuberculosis/immunology , Tuberculosis, Pulmonary/diagnosis , Adolescent , Antigens, Bacterial/immunology , Bacterial Proteins/immunology , Case-Control Studies , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Female , Humans , Male , Sensitivity and Specificity , Tuberculosis, Pulmonary/immunology
7.
Mem. Inst. Oswaldo Cruz ; 103(4): 401-404, June 2008. graf, tab
Article in English | LILACS | ID: lil-486859

ABSTRACT

The aim of this work was to study the difference in interferon gamma (IFN-gamma) production by T lymphocytes after early secretory antigen target 6 (ESAT-6) or purified protein derivate (PPD) stimulation in whole blood culture supernatants from children with suspected tuberculosis (TB) disease (n = 21), latent TB infection (n = 16) and negative controls (NC) (n = 22) from an endemic area in Brazil. The concentration of IFN-gamma (pg/ml) was measured by enzyme linked immunosorbent assay and the differences in the IFN-gamma levels for each group were compared and evaluated using an unpaired Student's t-test; p values < 0.05 were considered significant. Measurement of IFN-gamma levels after ESAT-6 stimulation raised the possibility of early diagnosis in the latent TB group (p = 0.0030). Nevertheless, the same group showed similar responses to the NC group (p > 0.05) after PPD stimulation. The IFN-gamma assay using ESAT-6 as an antigenic stimulus has the potential to be used as a tool for the immunodiagnosis of early TB in children.


Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Antigens, Bacterial , Bacterial Proteins , Interferon-gamma/biosynthesis , Mycobacterium tuberculosis/immunology , Tuberculosis, Pulmonary/diagnosis , Antigens, Bacterial/immunology , Bacterial Proteins/immunology , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Sensitivity and Specificity , Tuberculosis, Pulmonary/immunology
8.
Rev Saude Publica ; 42(2): 234-41, 2008 Apr.
Article in Portuguese | MEDLINE | ID: mdl-18327503

ABSTRACT

OBJECTIVE: To estimate the additional number of affected individuals based on the prevalence of sickle-cell syndromes among relatives of index cases. METHODS: Cross-sectional study of relatives of a random sample of index cases identified through a neonatal screening program in Northeastern Brazil, between 2001 and 2005. The extended family trial model included 463 relatives of 21 index cases. Relatives were classified as nuclear family (NF: father, mother, and siblings); first degree extended family (N1: grandparents, uncles and aunts, and first cousins); second degree extended family (N2: children of first cousins); extended family (NA: NF+N1+N2); and extended nuclear family (NA1: NF+N1). The presence of HBB*S and other abnormal hemoglobins was confirmed by high-performance liquid chromatography. The association between the presence of HBB*S and other variables was calculated using prevalence ratios and their respective 95% confidence intervals, and differences between means were calculated using Student's t test with a 5% significance level. RESULTS: Of relatives, 81% had no knowledge of sickle-cell anemia and HBB*S was present in 114 family members. A total of 53.3% of the studied population was considered as of reproductive age, and 80% of HBB*S carriers had already had children. Frequency was higher among NF (69%), but was also high in N1 (22.8%). NA1 screening resulted in the detection of 69 carriers additional (a 172% increase). CONCLUSIONS: These results indicate that family screening for the identification of sickle-cell carriers should be extended to first degree relatives.


Subject(s)
Family , Hemoglobin, Sickle/genetics , Mass Screening/methods , Sickle Cell Trait/epidemiology , Adolescent , Adult , Aged , Aged, 80 and over , Brazil/epidemiology , Child , Child, Preschool , Chromatography, High Pressure Liquid , Cross-Sectional Studies , Female , Humans , Infant , Male , Middle Aged , Prevalence , Sickle Cell Trait/diagnosis , Sickle Cell Trait/genetics
9.
J Parasitol ; 93(3): 712-4, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17626371

ABSTRACT

The conventional methods for identification and typing of Leishmania species depend on previous culture isolation of the parasites. Not infrequently, culture is unsuccessful and may result in misrepresentation of the heterogeneity of the original isolate. Thus, more reliable and precise identification of genotypes of Leishmania spp. is important for a better clinical and epidemiological understanding of the disease. We evaluated the potential of LSSP-PCR targeting kDNA minicircles in discriminating different variants of the parasite with the use of clinical samples directly or cultivated parasites. The 1st step of this procedure consists of the amplification of the minicircles by conventional PCR; the 2nd step is low-stringency amplification of the minicircles previously amplified, with the use of 1 of the primers. Although LSSP-PCR produced complex and distinct kDNA signatures for isolates representing different species, further experiments demonstrated that the approach had the potential for discriminating intraspecific variants of L. braziliensis. Thus, the generated profiles were too variable to be useful as markers for species identification. Moreover, we demonstrated that the approach can be directly applied to clinical samples. In conclusion, LSSP-PCR targeting kDNA minicircles produces profiles that reflect polymorphisms of the predominant classes of minicircles, and can be useful for studies aimed at discriminating Leishmania braziliensis genotypes without the need for previous cultivation of the parasite.


Subject(s)
DNA, Kinetoplast/analysis , Leishmania braziliensis/genetics , Polymerase Chain Reaction/methods , Animals , Cluster Analysis , DNA Primers , DNA, Kinetoplast/chemistry , Electrophoresis, Polyacrylamide Gel , Genetic Variation , Genotype , Humans , Leishmania braziliensis/classification , Leishmania braziliensis/isolation & purification , Leishmaniasis, Cutaneous/parasitology , Phylogeny , Reproducibility of Results , Species Specificity
10.
Rev. bras. hematol. hemoter ; 29(2): 179-184, abr.-jun. 2007.
Article in Portuguese | LILACS | ID: lil-467878

ABSTRACT

A anemia falciforme caracteriza-se como quadro hemolítico hereditário que evolui cronicamente causando danos físicos e emocionais às pessoas acometidas. Até o presente momento não se dispõe de tratamento curativo, a não ser o transplante de medula óssea, que ainda tem sido realizado de maneira experimental. A triagem neonatal de hemoglobinopatias, principalmente da anemia falciforme, tem sido essencial ao diagnóstico precoce e à instituição de medidas preventivas e promotoras de saúde. No entanto, o Ministério da Saúde do Brasil recomenda o exame dos pais a partir da identificação de heterozigotos, mas não faz alusão quanto à ampliação da triagem para outros familiares. Uma família que possua uma criança afetada com estas síndromes passa a ter um marcador para um grupo genético de risco. Neste caso, a triagem ampliada para os familiares mais próximos (avós, pais, irmãos, tios e primos) poderá identificar muitos portadores ou casais em risco, antes do casamento e procriação, além de servir de base a programas de assessoramento genético e de controle epidemiológico das hemoglobinopatias, uma herança genética bastante freqüente em nossa população.


Sickle cell anemia is a hereditary condition that evolves to a chronic illness, causing physical and emotional disorders to those involved. As yet there is no cure except for bone marrow transplantation which is still in the experimental stage. Neonatal screening for hemoglobin disorders, particularly sickle cell anemia, has been crucial for ensuring early diagnosis and the application of preventive and health-promoting measures. The Brazilian Health Ministry recommends testing parents thereby identifying heterozygotes, but does not propose extending this screening to other family members. A family that has a child affected by one of these syndromes is a marker for an at-risk group. In this case extending screning to close relatives (grandparents, siblings, aunts and uncles, and cousins) may identify individuals affected by the disease or couples at risk before marriage and reproduction and serve as the basis for programs providing genetic evaluation and epidemiological control of hemoglobin diseases that are relatively common in the Brazilian population.


Subject(s)
Anemia, Sickle Cell , Hemoglobin, Sickle , Family Characteristics , Bone Marrow Transplantation , Neonatal Screening , Hemoglobinopathies
11.
Mem Inst Oswaldo Cruz ; 102(2): 225-8, 2007 May.
Article in English | MEDLINE | ID: mdl-17426890

ABSTRACT

Patients with acute schistosomiasis were studied before and after oxamniquine treatment. They had been exposed to cercariae 5 to 9 weeks before, and presented compatible clinical manifestations, eosinophilia, and high levels of total IgE. Interferon-gamma (IFN-gamma) and interleukin-4 were measured by ELISA in whole blood samples under soluble egg antigen or soluble adult worm preparation stimulation. After treatment, the reduction of leukocytosis and eosinophilia were not significant, but total IgE levels decreased significantly, in contrast to IFN-gamma levels that were significantly increased. The oxamniquine treatment of acute schistosomiasis patients is followed by an improvement of a Th1 response in vitro. If this response has a protective aspect is unknown, and some investigations need to be realized.


Subject(s)
Antigens, Helminth/immunology , Interferon-gamma/biosynthesis , Oxamniquine/therapeutic use , Schistosoma mansoni/immunology , Schistosomiasis mansoni/drug therapy , Schistosomicides/therapeutic use , Acute Disease , Adolescent , Adult , Animals , Antibodies, Helminth/blood , Child , Disease Outbreaks , Enzyme-Linked Immunosorbent Assay , Feces/parasitology , Female , Humans , Immunoglobulin E/blood , Interferon-gamma/immunology , Male , Parasite Egg Count , Schistosomiasis mansoni/blood , Schistosomiasis mansoni/immunology
12.
Hemoglobin ; 31(1): 83-8, 2007.
Article in English | MEDLINE | ID: mdl-17365008

ABSTRACT

Various factors have been described as phenotypic modulators of sickle cell disease, such as levels of fetal hemoglobin (Hb F), presence of alpha-thalassemia (thal), and haplotypes of the beta-globin genes. In order to characterize and determine the frequency of the betaS and betaC mutations and the prevalence of -alpha3.7-thal, 74 patients with sickle cell disease detected during neonatal screening in the State of Pernambuco, Brazil, were studied. The haplotypes of the beta gene and -alpha3.7-thal were determined using polymerase chain reaction (PCR), and specific restriction endonucleases were used to establish the polymorphic sites of the haplotypes. The results showed the high frequency of the Central African Republic (CAR) or Bantu haplotype in the State of Pernambuco, Brazil. The low frequency of the Benin haplotype recorded in this study, in comparison with other states in northeast Brazil, suggests the diversity of origins of Afro-Brazilians in this region.


Subject(s)
Anemia, Sickle Cell/genetics , Black People/genetics , Globins/genetics , Haplotypes/genetics , Hemoglobin, Sickle/genetics , alpha-Thalassemia/genetics , beta-Thalassemia/genetics , Anemia, Sickle Cell/blood , Anemia, Sickle Cell/ethnology , Black People/ethnology , Brazil/ethnology , Child, Preschool , Female , Humans , Infant , Male , alpha-Thalassemia/ethnology , beta-Thalassemia/ethnology
13.
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 133-136, Oct. 2006. graf
Article in English | LILACS | ID: lil-441237

ABSTRACT

This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding the SSU rRNA were designed to amplify with high specificity DNA from Schistosoma mansoni, in a real time quantitative PCR system. The limit of detection of parasite DNA for the system was 10 fg of purified genomic DNA, that means less than the equivalent to one parasite cell (genome ~580 fg DNA). The efficiency was 0.99 and the correlation coefficient (R²) was 0.97. When different copy numbers of the target amplicon were used as standards, the assay could detect at least 10 copies of the specific target. The primers used were designed to amplify a 106 bp DNA fragment (Tm 83°C). The assay was highly specific for S. mansoni, and did not recognize DNA from closely related non-schistosome trematodes. The real time PCR allowed for accurate quantification of S. mansoni DNA and no time-consuming post-PCR detection of amplification products by gel electrophoresis was required. The assay is potentially able to quantify S. mansoni DNA (and indirectly parasite burden) in a number of samples, such as snail tissue, serum and feces from patients, and cercaria infested water. Thus, these PCR protocols have potential to be used as tools for monitoring of schistosome transmission and quantitative diagnosis of human infection.


Subject(s)
Animals , Humans , DNA, Helminth/analysis , Polymerase Chain Reaction/methods , Schistosoma mansoni/genetics , Schistosomiasis mansoni/diagnosis , Electrophoresis, Agar Gel , Sensitivity and Specificity
14.
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 145-148, Oct. 2006.
Article in English | LILACS | ID: lil-441239

ABSTRACT

The detection of specific DNA sequences by polymerase chain reaction (PCR) has proved extremely valuable for the analysis of genetic disorders and the diagnosis of a variety of infectious disease pathogens. However, the application to the detection of Schistosoma mansoni is rare, despite a recommendation of the World Health Organization that a major focus of research on schistosomiasis should be on the development and evaluation of new strategies and tools for control of the disease. In this context, a few studies were published for the detection of the parasite in snails, monitoring of cercariae in water bodies, and diagnosis of human infection. The present minireview describes sensitive and specific PCR based systems to detect S. mansoni, indicating possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection.


Subject(s)
Animals , Humans , Biomphalaria/parasitology , Fresh Water/parasitology , Polymerase Chain Reaction/methods , Schistosoma mansoni/genetics , Schistosomiasis/diagnosis , DNA, Helminth/analysis , Electrophoresis, Agar Gel , Sensitivity and Specificity , Schistosomiasis/parasitology
15.
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 331-332, Oct. 2006. graf
Article in English | LILACS | ID: lil-441269

ABSTRACT

Schistosoma mansoni infected C57Bl/6 inducible nitric oxide synthase (iNOS)-deficient and non-deficient malnourished mice, both fed a balanced controlled diet were studied. Interleukins, IL-4 and IL-10 responses to soluble egg antigens (SEA) 90 days after infection, were determined. Our results suggest that in iNOS deficient, malnourished mice, 90 days after of infection, nitric oxide has a downregulating effect on IL-4 and IL-10 production. We are currently investigating the biological significance of these findings.


Subject(s)
Animals , Male , Mice , /biosynthesis , /biosynthesis , Malnutrition/immunology , Nitric Oxide Synthase Type II/deficiency , Schistosomiasis mansoni/immunology , Antigens, Helminth/immunology , Disease Models, Animal , Ovum/immunology
16.
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 353-354, Oct. 2006. graf
Article in English | LILACS | ID: lil-441273

ABSTRACT

Evaluation of hepatic fibrosis is usually performed by histopathological examination of biopsies. However, this is an invasive and potentially dangerous procedure. Several studies have proposed serum biological markers of hepatic fibrosis. This communication evaluates the use of serum cytokines as markers of hepatic fibrosis in hepatitis C, schistosomiasis, and co-infection.


Subject(s)
Adult , Humans , Cytokines/blood , Hepatitis C/immunology , Liver Cirrhosis/parasitology , Schistosomiasis/immunology , Biomarkers/blood , Cytokines/immunology , Enzyme-Linked Immunosorbent Assay , Hepatitis C/complications , /blood , Liver Cirrhosis/immunology , Predictive Value of Tests , Reproducibility of Results , Schistosomiasis/complications , Transforming Growth Factor beta/blood , Tumor Necrosis Factor-alpha/blood
17.
Trans R Soc Trop Med Hyg ; 100(11): 1049-55, 2006 Nov.
Article in English | MEDLINE | ID: mdl-16620892

ABSTRACT

Primers targeting the gene encoding the small subunit rRNA were designed to amplify DNA from Schistosoma mansoni with high specificity. Three PCR systems were developed: conventional PCR, two-step nested PCR (NPCR) and single-tube nested PCR (STNPCR). The limits of detection of parasite DNA for the conventional PCR, NPCR and STNPCR were 10 pg, 0.1 fg and 1 fg, respectively. The assays were highly specific for S. mansoni and did not recognise DNA from closely related non-schistosome trematodes. Using pools of Biomphalaria molluscs, PCR, NPCR and STNPCR were positive in 6/16 (37.5%), 15/16 (93.8%) and 13/16 (81.3%) of the tested samples, respectively, whereas the observation of cercariae shedding after exposure to light was able to detect S. mansoni infection in 6/16 (37.5%) of the pools. Thus, the molecular detection systems had a higher level of sensitivity than standard screening of intermediate hosts by cercarial shedding when DNA was purified from pools of snails collected from endemic areas. These PCR protocols have potential to be used as tools for monitoring of schistosome transmission.


Subject(s)
Biomphalaria/parasitology , Schistosoma mansoni/parasitology , Schistosomiasis/parasitology , Animals , DNA, Helminth/analysis , Electrophoresis, Agar Gel , Humans , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/standards , Schistosomiasis/transmission , Sensitivity and Specificity
18.
Trends Parasitol ; 22(2): 85-91, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16380294

ABSTRACT

Studies in mice indicate that schistosome egg-induced granuloma formation and hepatic fibrosis depend markedly on cytokine regulation, with interleukin 10 having a central role. There is no clear consensus about the pattern of cytokine production and regulation that causes a minority of chronically exposed patients to develop severe hepatosplenic (HS) disease, which is characterized by periportal fibrosis and portal hypertension. HS disease and the progression of hepatic fibrosis are associated with the production of profibrotic type 2 cytokines in the early stages of infection with Schistosoma mansoni. However, other studies indicate that HS disease is characterized by a predominant T helper 1 profile. Until new tools and approaches are developed to study human disease in endemic areas, investigators must either speculate about indirect evidence from human studies or rely more heavily on findings generated from experimental models of the disease.


Subject(s)
Host-Parasite Interactions/immunology , Liver Diseases/parasitology , Schistosomiasis/immunology , Schistosomiasis/pathology , Splenic Diseases/parasitology , Animals , Disease Models, Animal , Humans , Interleukin-10/immunology , Liver Cirrhosis/immunology , Liver Cirrhosis/parasitology , Liver Diseases/immunology , Liver Diseases/pathology , Mice , Splenic Diseases/immunology , Splenic Diseases/pathology
19.
Mem Inst Oswaldo Cruz ; 101 Suppl 1: 133-6, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17308760

ABSTRACT

This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding the SSU rRNA were designed to amplify with high specificity DNA from Schistosoma mansoni, in a real time quantitative PCR system. The limit of detection of parasite DNA for the system was 10 fg of purified genomic DNA, that means less than the equivalent to one parasite cell (genome ~580 fg DNA). The efficiency was 0.99 and the correlation coefficient (R(2)) was 0.97. When different copy numbers of the target amplicon were used as standards, the assay could detect at least 10 copies of the specific target. The primers used were designed to amplify a 106 bp DNA fragment (Tm 83 degrees C). The assay was highly specific for S. mansoni, and did not recognize DNA from closely related non-schistosome trematodes. The real time PCR allowed for accurate quantification of S. mansoni DNA and no time-consuming post-PCR detection of amplification products by gel electrophoresis was required. The assay is potentially able to quantify S. mansoni DNA (and indirectly parasite burden) in a number of samples, such as snail tissue, serum and feces from patients, and cercaria infested water. Thus, these PCR protocols have potential to be used as tools for monitoring of schistosome transmission and quantitative diagnosis of human infection.


Subject(s)
DNA, Helminth/analysis , Polymerase Chain Reaction/methods , Schistosoma mansoni/genetics , Schistosomiasis mansoni/diagnosis , Animals , Electrophoresis, Agar Gel , Humans , Sensitivity and Specificity
20.
Mem Inst Oswaldo Cruz ; 101 Suppl 1: 145-8, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17308762

ABSTRACT

The detection of specific DNA sequences by polymerase chain reaction (PCR) has proved extremely valuable for the analysis of genetic disorders and the diagnosis of a variety of infectious disease pathogens. However, the application to the detection of Schistosoma mansoni is rare, despite a recommendation of the World Health Organization that a major focus of research on schistosomiasis should be on the development and evaluation of new strategies and tools for control of the disease. In this context, a few studies were published for the detection of the parasite in snails, monitoring of cercariae in water bodies, and diagnosis of human infection. The present minireview describes sensitive and specific PCR based systems to detect S. mansoni, indicating possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection.


Subject(s)
Biomphalaria/parasitology , Fresh Water/parasitology , Polymerase Chain Reaction/methods , Schistosoma mansoni/genetics , Schistosomiasis/diagnosis , Animals , DNA, Helminth/analysis , Electrophoresis, Agar Gel , Humans , Schistosomiasis/parasitology , Sensitivity and Specificity
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