Inference of stature using segmental measures in comparison with directly measured height in children and adolescents: an analytical cross-sectional study.

OBJECTIVES: In the clinical routine of pediatricians, height is the most reliable indicator for assessing growth. However, there are situations where it is not possible to measure this parameter directly, making the estimation of height or length a useful alternative. The main goal of this study is to identify which segmental measure, including upper arm length (UAL), tibial length (TL), and knee-heel length (KHL), provides the stature estimate that most closely approximates directly measured height in the study participants. METHODS: Analytical cross-sectional study of the anthropometric and segmental measures of 248 participants, aged 0 to 14 years old, using Stevenson's and Kihara's equations to estimate indirectly measured height. RESULTS: The segmental measure that provided a measurement that deviated the least from the actual height was the KHL, followed by TL, both calculated using Stevenson's equations. CONCLUSION: The use of segmental measures to infer a child's stature is valuable in clinical practice, particularly in bedridden and incapacitated patients. Based on the present findings, the KHL and TL segments yielded more accurate results than the UAL.

Relação entre alteração de cor na coroa e necrose pulpar em dentes decíduos: relato de casos / Relationship between color change in the crown and pulp necrosis in primary teeth: case reports

Objetivo: avaliar e discutir a relação entre alteração de cor na coroa e necrose pulpar em dentes decíduos, baseado em dois relatos de casos. Relato dos casos: em ambos os casos os pacientes do gênero feminino, 4 anos de idade, compareceram a um centro de referência de traumatismos dentários com escurecimento nos incisivos decíduos. O caso 1 trata-se de um traumatismo classificado como subluxação ocorrido há mais de dois anos. No exame clínico foi observada alteração de cor nos elementos 51 e 61 e no exame radiográfico não foi verificada imagem sugestiva de lesão periapical. No caso 2, diagnosticado como concussão, clinicamente foi observada alteração de cor e no exame radiográfico verificou-se uma reabsorção radicular associada à lesão periapical no elemento 61. Conclusão: o escurecimento de um dente decíduo após um traumatismo dentário nem sempre é indicativo de necrose pulpar. Sugere-se o acompanhamento periódico da presença de outros fatores, como fístula, abscesso ou lesão apical que irão auxiliar em um correto diagnóstico. Cada caso deve ser averiguado de forma independente tendo em vista que o sucesso do tratamento está vinculado ao acompanhamento periódico e ao diagnóstico de sinais radiográficos e clínicos de alterações decorrentes do episódio traumático.

Objective: the aim of this study was to evaluate and discuss the relationship between color change in the crown and pulp necrosis in deciduous teeth, based on two case reports. Case Report: both cases were female patients, 4 years old, who came to a reference center for dental trauma with complaints of darkening of the deciduous incisors. The case 1 was a trauma classified as subluxation that occurred more than two years ago. In the clinical examination a color change was observed in element 51 and 61, and in the radiographic examination no image suggestive of periapical lesion was seen. In case 2, diagnosed as concussion, clinically the color change was observed, and radiographic examination showed root resorption associated with periapical lesion in element 61. Conclusion: thus, according to the literature, we conclude that the darkening of a deciduous tooth after dental trauma is not always indicative of pulp necrosis. Periodic monitoring for the presence of other factors, such as fistula, abscess or apical lesion that will help in a correct diagnosis is suggested. Each case must be evaluated independently, since treatment success is linked to periodic monitoring and diagnosis of radiographic and clinical signs of changes resulting from the traumatic episode.

Morphometry of cryopreserved organs from the gastrointestinal system of squirrel monkeys (Saimiri sciureus, Linnaeus, 1758) for improvement of management.

BACKGROUND: Nonhuman primates (NHPs) are fundamental for countless types of research and their phylogenetic similarities with humans make them valuable biomodels. Saimiri (squirrel monkeys) is the New World simian genus that is most widely used in research; however, little is known about the morphology of the gastrointestinal tract (GIT) of these animals. METHODS: The morphological and topographical aspects of GIT organs from 14 previously frozen carcasses of captive squirrel monkeys (Saimiri sciureus) were described as a means for refining clinical and laboratory diagnoses, with the aim of providing a basis for clinical assessments and animal management. The carcasses were gradually thawed, macroscopically evaluated for their appearance, measured, weighed, photographed, and necropsied. The characteristics of all the GIT organs (main and accessories) were evaluated, with measurement of the dimensions and description of the shape, consistency, and color of each one. RESULTS: The stomach of squirrel monkeys is simple and of non-compartmentalized type and the cecum does not have numerous folds, compared with other species. These characteristics are compatible with omnivorous simian species with no adaptive digestive specialization for the food fermentation process. Significant correlations were found between the weights of the carcass and GIT (p < .05); carcass and tongue (0.83; p < .01); and liver and right salivary gland (0.83; p < .01). It was also observed that the animals showed good body score conformation and GIT organs in good condition, which indicated that their nutritional management had been adequate. Regarding the correlations found among the organs: (i) squirrel monkeys with heavier carcasses had better-developed GIT with greater food storage capacity; (ii) the bigger the animal was, the greater its overall muscle development was, including that of the tongue, which would enable intensification of its feeding activity; and (iii) the measurements of the esophagus, small and large intestines, pancreas, and salivary glands were not sex-dependent in Saimiri sciureus. CONCLUSIONS: Previously cryopreserved carcasses of squirrel monkeys can be used for gastrointestinal system studies, with the potential to make clinical, zootechnical, management, and scientific contributions.

The Sedentary Lifestyle and Masticatory Dysfunction: Time to Review the Contribution to Age-Associated Cognitive Decline and Astrocyte Morphotypes in the Dentate Gyrus.

As aging and cognitive decline progresses, the impact of a sedentary lifestyle on the appearance of environment-dependent cellular morphologies in the brain becomes more apparent. Sedentary living is also associated with poor oral health, which is known to correlate with the rate of cognitive decline. Here, we will review the evidence for the interplay between mastication and environmental enrichment and assess the impact of each on the structure of the brain. In previous studies, we explored the relationship between behavior and the morphological features of dentate gyrus glial fibrillary acidic protein (GFAP)-positive astrocytes during aging in contrasting environments and in the context of induced masticatory dysfunction. Hierarchical cluster and discriminant analysis of GFAP-positive astrocytes from the dentate gyrus molecular layer revealed that the proportion of AST1 (astrocyte arbors with greater complexity phenotype) and AST2 (lower complexity) are differentially affected by environment, aging and masticatory dysfunction, but the relationship is not straightforward. Here we re-evaluated our previous reconstructions by comparing dorsal and ventral astrocyte morphologies in the dentate gyrus, and we found that morphological complexity was the variable that contributed most to cluster formation across the experimental groups. In general, reducing masticatory activity increases astrocyte morphological complexity, and the effect is most marked in the ventral dentate gyrus, whereas the effect of environment was more marked in the dorsal dentate gyrus. All morphotypes retained their basic structural organization in intact tissue, suggesting that they are subtypes with a non-proliferative astrocyte profile. In summary, the increased complexity of astrocytes in situations where neuronal loss and behavioral deficits are present is counterintuitive, but highlights the need to better understand the role of the astrocyte in these conditions.

Function Over Mass: A Meta-Analysis on the Importance of Skeletal Muscle Quality in COVID-19 Patients.

COVID-19 caused by SARS-CoV-2 infection is a highly contagious disease affecting both the higher and lower portions of the respiratory tract. This disease reached over 265 million people and has been responsible for over 5.25 million deaths worldwide. Skeletal muscle quality and total mass seem to be predictive of COVID-19 outcome. This systematic review aimed at providing a critical analysis of the studies published so far reporting on skeletal muscle mass in patients with COVID-19, with the intent of examining the eventual association between muscle status and disease severity. A meta-analysis was performed to evaluate whether skeletal muscle quantity, quality and function were related to disease severity. Systematic reviews and meta-analyses were conducted according to the guidelines of the Cochrane Handbook for Systematic Reviews of Interventions and reported according to the guidelines of the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guide. From a total of 1,056 references found, 480 were selected after removing duplicates. Finally, only 7 met the specified inclusion criteria. The results of this meta-analysis showed that skeletal muscle quality, rather than quantity, was associated with COVID-19 severity, as confirmed by lower skeletal muscle density and lower handgrip strength in patients with severe disease. Muscle function assessment can thus be a valuable tool with prognostic value in COVID-19.

Retenção do conhecimento sobre saúde bucal em uma amostra de escolares de Nova Friburgo / Knowledge retention about oral health in a sample of school children from Nova Friburgo

As medidas de orientação para a prevenção da cárie apoiam-se na educação em saúde bucal do paciente. Este trabalho avaliou a retenção do conhecimento sobre saúde bucal em escolares, antes e depois da realização de oficinas educativas. Após a aprovação no comitê de ética em pesquisa (CEP Nº2.987.965) este estudo foi realizado com crianças do ensino fundamental de uma escola pública em Nova Friburgo-RJ. Um questionário sobre práticas de higiene bucal e dieta, e conhecimento sobre as doenças bucais foi desenvolvido e aplicado antes da realização de oficinas educativas em saúde bucal e 15, 30, 60 e 90 dias após. A amostra foi de 136 escolares, 54,4% meninos e 45,6% meninas, com média de idade de 8,7 anos. Antes das oficinas as crianças relataram limpar seus dentes todos os dias (91,2%), pelo menos três vezes ao dia (60,3%), utilizando escova, pasta e fio dental (52,9%); 59,6% das crianças relataram saber o que causava a cárie antes das oficinas; 15 e 30 dias após as oficinas, os percentuais foram de 94,9% e 98,5%, respectivamente (p<0,05), diminuindo para 58,8% após 60 dias. Para a gengivite, apenas 11,8% das crianças relataram saber a causa da doença antes das oficinas; 15 e 30 dias após os percentuais foram de 81,6% e 94,9%, respectivamente, diminuindo para 13,2% após 60 dias. Com base nos resultados observou-se uma diminuição na retenção do conhecimento após 30 dias da realização das atividades educativas, sugerindo que esta população deva receber um programa de educação em saúde bucal de forma continuada.

Guidance measures for the prevention of caries are supported by education in the patient's oral health. This study evaluated the retention of knowledge about oral health in schoolchildren before and after educational workshops. After approval by the Research Ethics Committee (CEP No. 2,987,965) the study was carried out with elementary school children from a public school in Nova Friburgo. A questionnaire on hygiene and dietary practices and knowledge about oral diseases was applied before educational workshops on oral health and 15, 30, 60 and 90 days after. The sample consisted of 136 students, 54.4% boys and 45.6% girls, with an average age of 8.7 years. Before the workshops children reported cleaning their teeth every day (91.2%), at least three times a day (60.3%), using a brush, toothpaste and dental floss (52.9%); 59.6% of children reported knowing what caused decay before participating in the workshops; 15 and 30 days after the workshops the percentages were 94.9% and 98.5%, respectively (p <0.05), decreasing to 58.8% 60 days after. For gingivitis, only 11.8% of children reported knowing the cause of the disease before the workshops; 15 and 30 days after the percentages were 81.6% and 94.9%, respectively, decreasing to 13.2% after 60 days. Based on the results, there was a decrease in knowledge retention after 30 days of educational activities, suggesting that this population should receive an oral health education program on an ongoing basis.

Eficácia do método de graham para o diagnóstico de Syphacia obvelata (Rudolphi, 1802) no monitoramento sanitário de camundongos Swiss Webster de um biotério de criação / Effectiveness of the graham method for Syphacia obvelata (Rudolphi, 1802) diagnosis in the health monitoring of Swiss Webster mice in a breeding colony

A infecção por Oxyuridae constitui um problema nas barreiras sanitárias em colônias de roedores, tendo em vista que esta é a família de parasito mais encontrada em instalações de animais de laboratório, especialmente a espécie Syphacia obvelata. Camundongos outbred stock Swiss Webster apresentam sinais clínicos quando associados com elevada carga parasitária e, geralmente, é observado um aumento no número de animais com prolapso retal. O controle desse parasito e outros patógenos é realizado mediante monitoramento sanitário trimestral, conforme as recomendações da Federação das Associações Europeias de Ciência de Animais de Laboratório (FELASA). O diagnóstico pelo método de Graham é determinante para a pesquisa dos ovos através de impressão de fita adesiva na região perianal do animal no início do ciclo do oxiurídeo. Foi realizada uma análise comparativa entre os métodos de Graham e o exame direto da mucosa intestinal para diagnóstico de S. obvelata de 60 camundongos sentinelas fêmeas outbred stock Swiss webster, procedentes de instalações de roedores de um biotério de criação. Os resultados mostraram uma diferença significativa entre o método de Graham e o exame direto da mucosa intestinal (p > 0,001 a 95%, teste de Fisher exato), com uma prevalência de 50% de ovos do parasito pela técnica de Graham e 8,33% pelo exame direto da mucosa intestinal, que detectou todas as formas do parasito. Pelo exame direto, além de ovos, foram identificadas outras formas do parasito, como: larvas, fêmeas adultas e machos adultos. Concluiu-se que a técnica de Graham é capaz de ampliar o espectro de identificação de S. obvelata, pois detecta com maior precisão a presença de ovos do parasita, uma vez que a fita adesiva demonstra que as fêmeas adultas grávidas migram para o ânus antes de morrer, depositando seus ovos na região perianal do hospedeiro. Sendo assim, o método de Graham deve ser adicionado ao programa de monitoramento sanitário, associado aos outros rotineiramente empregados no controle da qualidade de animais de laboratório, contribuindo com as condutas de manejo da criação animal em prol do controle e erradicação do agente em questão.

Infection by Oxyuridae is a problem in sanitary barriers in rodent colonies, considering that this is the parasite family most commonly found in laboratory animal facilities, especially the species Syphacia obvelata. Outbred stock Swiss Webster mice show clinical signs when associated with a high parasite load and, generally, an increase in the number of animals with rectal prolapse is observed. The control of this parasite and other pathogens is carried out through quarterly sanitary monitoring, in accordance with the recommendations of the Federation of European Associations of Laboratory Animal Science (FELASA). Diagnosis by Graham's method is crucial for the search for eggs by printing adhesive tape in the animal's perianal region at the beginning of the oxyuride cycle. A comparative analysis was performed between Graham's methods and direct examination of the intestinal mucosa for the diagnosis of S. obvelata in 60 female sentinel Swiss outbred stock Swiss webster mice, from rodent installations in a breeding animal facility. Results showed a significant difference between Graham's method and direct examination of the intestinal mucosa (p > 0.001 at 95%, Fisher's exact test), with a prevalence of 50% of parasite eggs by Graham's technique and 8.33% by direct examination of the intestinal mucosa, which detected the whole parasite forms. By direct examination, in addition to eggs, other forms of the parasite were identified, such as: larvae, adult females and adult males. It was concluded that Graham's technique is capable of expanding the identification spectrum of S. obvelata, as it detects the presence of parasite eggs more accurately, since the adhesive tape demonstrates that adult pregnant females migrate to the anus before dying, depositing its eggs in the perianal region of the host. Therefore, Graham's method should be added to the health monitoring program, associated with others routinely used in the quality control of laboratory animals, contributing to the management of animal husbandry in favor of the control and eradication of the agent in question.

Estudo de evidências de validade discriminante para o Desenho da Família Cinética / Study of evidence of discriminant validity for the Kinetic Family Drawing Test / Estudio de evidencias de validez discriminante para el Dibujo de la Familia Kinética

Esta pesquisa teve como objetivo principal investigar evidências de validade discriminante para o Desenho da Família Cinética. Pretendeu também analisar a influência de variáveis sociodemográficas, bem como a frequência e capacidade de discriminação de cada item de análise dessa técnica projetiva. Participaram 112 crianças de 9 a 12 anos pertencentes a dois grupos critério, sendo 80 da amostra normativa e 32 da amostra clínica (com problemas de aprendizagem). Os instrumentos utilizados foram o Desenho da Família Cinética, sua folha de correção e as Matrizes Progressivas e Coloridas de Raven. Os dados foram analisados por meio de estatísticas descritivas e inferenciais com o auxílio do software SPSS. Os principais resultados mostraram que a variável sociodemográfica Idade gerou efeito nos resultados obtidos; houve diferença estatisticamente significativa entre os grupos-critério, corroborando para as evidências de validade discriminante; e apenas 38,46% dos itens de análise obtiveram diferença estatisticamente significativa entre os grupos critério. (AU)

This study aimed to investigate evidence of discriminant validity for the Kinetic Family Drawing test. It also intended to analyze the influence of sociodemographic variables, to evaluate the frequency and discriminatory capacity of each analysis item of this projective technique. Participants were 112 children from 9 to 12 years of age, belonging to two criterion groups, 80 from the normative sample and 32 from the clinical sample (with learning problems). The instruments used were the Kinetic Family Drawing test, its correction sheet and Raven's Progressive and Colored Matrices. Data were analyzed through descriptive and inferential statistics using the SPSS software. The main results showed that the sociodemographic variable age influenced the results obtained; there was a statistically significant difference between the criterion groups, corroborating the evidence of discriminant validity; while only 38.46% of the analysis items obtained statistically significant difference between the criterion groups. (AU)

Esta investigación tuvo como objetivo principal investigar las evidencias de validez discriminatorias para el Dibujo de la Familia Kinética. También se pretendía analizar la influencia de las variables sociodemográficas, así como evaluar la frecuencia y la capacidad discriminatoria de cada ítem de análisis de esta técnica proyectiva. Participaron un total de 112 niños de 9 a 12 años, pertenecientes a dos grupos de criterios, 80 de la muestra normativa y 32 de la muestra clínica (con problemas de aprendizaje). Los instrumentos utilizados fueron el Dibujo de la Familia Kinética, su hoja de corrección y las Matrices Progresivas Coloreadas de Raven. Los datos se analizaron mediante estadística descriptiva e inferencial con la ayuda del software SPSS. Los principales resultados mostraron que la variable sociodemográfica edad tuvo un efecto sobre los resultados obtenidos; hubo una diferencia estadísticamente significativa entre los grupos de criterios, corroborando la evidencia de validez discriminante; y solo el 38.46% de los ítems de análisis obtuvieron diferencias estadísticamente significativas entre los grupos de criterios. (AU)

Omega-3 Fatty Acid Supplementation and Its Impact on Systemic Inflammation and Body Weight in Patients With Cancer Cachexia-A Systematic Review and Meta-Analysis.

Body weight loss and inflammation are major alterations related to cancer cachexia, an important wasting syndrome highly prevalent in many types of cancer. Nutritional components modulate inflammation in several chronic diseases. Omega-3 fatty acids (n-3) are well known for their anti-inflammatory properties. However, the effects of n-3 on cancer cachexia are still controversial. This systematic review and meta-analysis aims to evaluate the reported effects of n-3 supplementation on body weight and inflammatory markers in patients with cancer cachexia. Articles indexed in the major scientific platforms were retrieved in agreement with the Preferring Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) and 167 references were initially found. After removing duplicates and applying the inclusion and exclusion criteria, this systematic review included six studies. Using a random-effects model with 95% CI, three effect sizes were expressed as standard mean difference (SMD). No differences were found regarding the effect of n-3 on interleukin-6, C-reactive protein, and albumin levels. Body weight analysis included only two studies, devoid of robust conclusions. The low number of studies, low sample size, and great intra-variability precluded a stronger analysis. More studies evaluating n-3 supplementation in cancer cachexia are still needed.

Coronavirus Disease 2019 (COVID-19) and Nutritional Status: The Missing Link?

Coronavirus disease 2019 (COVID-19) is an emerging disease that has reached pandemic status by rapidly spreading worldwide. Elderly individuals and patients with comorbidities such as obesity, diabetes, and hypertension show a higher risk of hospitalization, severe disease, and mortality by acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. These patients frequently show exacerbated secretion of proinflammatory cytokines associated with an overreaction of the immune system, the so-called cytokine storm. Host nutritional status plays a pivotal role in the outcome of a variety of different infectious diseases. It is known that the immune system is highly affected by malnutrition, leading to decreased immune responses with consequent augmented risk of infection and disease severity. Body composition, especially low lean mass and high adiposity, has consistently been linked to worsened prognosis in many different diseases. In this review, evidence concerning the impact of nutritional status on viral infection outcomes is discussed.

Frequency of pulp canal obliteration in primary teeth after traumatic dental injury and its association with related variables / Frequência de obliteração do canal pulpar nos dentes decíduos após traumatismo dentário e sua associação com variáveis relacionadas

ABSTRACT Objective The aim of this study was to determine the frequency of pulp canal obliteration (PCO) after traumatic dental injury (TDI) of primary anterior teeth and to investigate its relation with the related variables. Methods This retrospective study has been done with dental records of patients selected from the Paediatric Dental Trauma Clinic between 2006 and 2016. Those who had PCO in the primary anterior teeth and were aged 0-108 months at the time of trauma were considered eligible. Data related to patients and TDI, such as sex, the child's age at the time of trauma, aetiology, affected teeth, the child's age at the time of the eruption of the permanent successor as well as the presence of crown discoloration were extracted from the dental records. Results Among the 483 children with traumatised teeth, 14.9% had PCO and the most of them exhibited crown discoloration. The average age of the children at the time of the trauma was 38 months and the most common aetiology of the TDI was falls. The average time for the beginning of the PCO process was 13.5 months. There was no statistically significant association between the child's age at the time of trauma and the types of TDI, PCO and the presence of crown discoloration. Conclusions The frequency of PCO was relatively low though the presence of crown discoloration was considerable. There was no association between the child's age at the time of trauma and the studied variable.

RESUMO Objetivo O objetivo desse estudo foi determinar a frequência de obliteração do canal pulpar (OCP) após traumatismo dos dentes decíduos anteriores e investigar sua associação com variáveis relacionadas. Métodos Este estudo retrospectivo foi realizado com base nos prontuários odontológicos de pacientes selecionados da Clínica de Trauma Dentário Pediátrico entre 2006 e 2016. Aqueles que tinham OCP nos dentes decíduos anteriores e tinham entre 0-108 meses no momento do traumatismo foram considerados elegíveis. Dados dos pacientes e dos traumatismos, tais como, sexo, idade da criança no momento do traumatismo, etiologia, dente afetado, idade da criança na época no momento da erupção do sucessor permanente, bem como presença de alteração de cor da coroa foram extraídos dos prontuários. Resultados Dentre as 483 crianças com dentes com traumatismos, 14.9% tinham OCP e a maioria delas apresentava alteração de cor da coroa. A média de idade da criança no momento do traumatismo foi de 38 meses e a etiologia mais comumente associada ao TD foram as quedas. O tempo médio decorrido para o início do processo de OCP foi de 13,5 meses. Não houve associação estatisticamente significativa entre a idade da criança no momento do traumatismo e os tipos de TD, OCP e presença de alteração de cor da coroa. Conclusão A frequência de OCP foi relativamente baixa, embora a presença de alteração da coroa tenha sido considerável. Não foi encontrada associação entre a idade da criança no momento do trauma e as variáveis estudadas.

Low Aedes aegypti Vector Competence for Zika Virus from Viremic Rhesus Macaques.

Despite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of Aedes aegypti fed directly on viremic human and non-human primates (NHPs). We blood-fed Ae. aegypti from two districts in Rio de Janeiro on six ZIKV infected pregnant rhesus macaques at several time points, half of which were treated with Sofosbuvir (SOF). Mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. Although viremia extended up to eight days post monkey inoculation, only mosquitoes fed on the day of the peak of viremia, recorded on day two, became infected. The influence of SOF treatment could not be assessed because the drug was administered just after mosquito feeding on day two. The global infection, dissemination and transmission rates were quite low (4.09%, 1.91% and 0.54%, respectively); no mosquito was infected when viremia was below 1.26 × 105 RNA copies/mL. In conclusion, Ae. aegypti vector competence for ZIKV from macaques is low, likely to be due to low viral load and the short duration of ZIKV viremia in primates suitable for infecting susceptible mosquitoes. If ZIKV infection in human and macaques behaves similarly, transmission of the Zika virus in nature is most strongly affected by vector density.

Sofosbuvir shows a protective effect against vertical transmission of Zika virus and the associated congenital syndrome in rhesus monkeys.

The outbreaks of Zika virus (ZIKV) infection in Brazil, 2015-2016, were associated with severe congenital malformations. Our translational study aimed to test the efficacy of the antiviral agent sofosbuvir (SOF) against vertical transmission of ZIKV and the associated congenital syndrome (CZS), using a rhesus monkey model. Eight pregnant macaques were successfully infected during the organogenesis phase with a Brazilian ZIKV strain; five of them received SOF from two to fifteen days post-infection. Both groups of dams showed ZIKV-associated clinical signals, detectable ZIKV RNA in several specimens, specific anti-ZIKV IgM and IgG antibodies, and maternal neutralizing antibodies. However, malformations occurred only among non-treated dam offspring. Compared to non-treated animals, all SOF-treated dams had a shorter ZIKV viremia and four of five neonates had undetectable ZIKV RNA in blood and tissue samples. These results support further clinical evaluations aiming for the prevention of CZS.

Estudos de fidedignidade para o Desenho da Família Cinética / Reliability studies for the Kinetic Family Drawing / Estudios de confiabilidad para el Dibujo Kinético de la Familia

Esta pesquisa teve como objetivo realizar estudos de fidedignidade para o Desenho da Família Cinética, sendo estes relativos aos parâmetros de fidedignidade entre avaliadores, intra-avaliador e estabilidade temporal/teste-reteste. Os instrumentos utilizados foram o Desenho da Família Cinética e o Teste das Matrizes Progressivas Coloridas de Raven. Participaram desses estudos dois juízes e 66 crianças, sendo 16 com problemas de aprendizagem. Para a análise de dados, utilizou-se o coeficiente kappa de Cohen, a correlação ρ de Spearman e a diferença estatística entre as médias dos pontos por meio do Teste Wilcoxon. Os resultados mostraram índices substanciais a perfeitos de concordância nos estudos de fidedignidade entre avaliadores e intra-avaliador para todos os itens de análise do Desenho da Família Cinética e, no teste-reteste, não houve diferenças estatisticamente significativas entre as médias das pontuações entre as duas aplicações. Conclui-se pela robustez psicométrica do Desenho da Família Cinética, em termos de indicadores de fidedignidade.(AU)

This study aimed to perform reliability analyses for the Kinetic Family Drawing, in relation to inter- and intra-rater reliability parameters and temporal/test-retest stability. The instruments used were the Kinetic Family Drawing and the Raven Colored Progressive Matrix Test. Twelve judges and 66 children participated in this study, 16 of whom had learning problems. For the data analysis, Cohen's kappa coefficient, Spearman's correlation coefficient ρ, and the statistical difference between the means of the points through the Wilcoxon test were used. The results showed substantial perfect agreement indices in the inter- and intra-rater reliability analyses for all items of the Kinetic Family Drawing, and in the test-retest there were no statistically significant differences between the mean scores of the two applications. The psychometric robustness of the Kinetic Family Drawing, in terms of reliability indicators, was confirmed.(AU)

Esta investigación tuvo como objetivo realizar estudios de confiabilidad para el Dibujo Kinético de la Familia, que se relaciona con los parámetros de referencia entre los evaluadores, intra-evaluadores y estabilidad temporal/test-retest. Los instrumentos utilizados fueron el Dibujo Kinético de la Familia y la Test de Matrices Progresivas de Raven. Participaron de los estudios 2 jueces y 66 niños, siendo que 16 de ellos poseían problemas de aprendizaje. Para el análisis de los datos, se utilizó el coeficiente kappa de Cohen, la correlación de Spearman ρ y la diferencia estadística entre las medias de los puntos por medio de la prueba de Wilcoxon. Los resultados mostraron índices de concordancia considerables en los estudios de confiabilidad entre evaluadores e intra-evaluadores para todos los ítems de análisis del Dibujo Kinético de la Familia y, en el test-retest, no hubo diferencias estadísticamente significativas entre las medias de las puntuaciones entre las dos aplicaciones. Se concluye por la robustez psicométrica del Dibujo Kinético de la Familia, en términos de indicadores de confiabilidad.(AU)

STATURE ESTIMATE OF CHILDREN WITH CEREBRAL PALSY THROUGH SEGMENTAL MEASURES: A SYSTEMATIC REVIEW.

OBJECTIVE: To review studies that evaluate the correspondence between the estimate height via segmental measures and the actual height of children with cerebral palsy. DATA SOURCES: Systematic literature review between 1995-2018, guided by the PRISMA criteria (Preferred Reporting Items for Systematic Reviews and Meta-Analyses), in PubMed, BVS, MEDLINE and Lilacs databases. The descriptors, connected by the AND Boolean Operators, were: anthropometry, cerebral palsy, child and body height. The research comprised papers in Portuguese, English and Spanish, with Qualis-CAPES equal or superior to B3 that addressed the question: "Is there any correlation between estimate height by equations and direct height measures in children with cerebral palsy?" 152 studies were recovered and seven were selected. Their methodological quality was assessed by the scale of the Agency for Healthcare Research and Quality (AHRQ). DATA SYNTHESIS: Most studies showed no correspondence between estimated and real height. Studies that showed coincidence of the measures contain limitations that could jeopardize the results (sample losses, small samples and exclusion of patients with severe contractures, scoliosis and severe cerebral palsy). Japanese researchers developed an equation which harmoniously aligns the statures; the study comprised only Japanese patients, though. CONCLUSIONS: Given the importance of accuracy in height measures to evaluate infant health, it is crucial to carry out more researches in order to safely establish an association between both estimate and real statures. The development of anthropometric protocols, emerged from such researches, would benefit the follow-up of children with severe psychomotor disabilities.

Stature estimate of children with cerebral palsy through segmental measures: a systematic review / Estimativa de estatura em crianças com paralisia cerebral por meio de medidas segmentares: uma revisão sistemática

ABSTRACT Objective: To review studies that evaluate the correspondence between the estimate height via segmental measures and the actual height of children with cerebral palsy. Data sources: Systematic literature review between 1995-2018, guided by the PRISMA criteria (Preferred Reporting Items for Systematic Reviews and Meta-Analyses), in PubMed, BVS, MEDLINE and Lilacs databases. The descriptors, connected by the AND Boolean Operators, were: anthropometry, cerebral palsy, child and body height. The research comprised papers in Portuguese, English and Spanish, with Qualis-CAPES equal or superior to B3 that addressed the question: "Is there any correlation between estimate height by equations and direct height measures in children with cerebral palsy?" 152 studies were recovered and seven were selected. Their methodological quality was assessed by the scale of the Agency for Healthcare Research and Quality (AHRQ). Data synthesis: Most studies showed no correspondence between estimated and real height. Studies that showed coincidence of the measures contain limitations that could jeopardize the results (sample losses, small samples and exclusion of patients with severe contractures, scoliosis and severe cerebral palsy). Japanese researchers developed an equation which harmoniously aligns the statures; the study comprised only Japanese patients, though. Conclusions: Given the importance of accuracy in height measures to evaluate infant health, it is crucial to carry out more researches in order to safely establish an association between both estimate and real statures. The development of anthropometric protocols, emerged from such researches, would benefit the follow-up of children with severe psychomotor disabilities.

RESUMO Objetivo: Revisar estudos que avaliam correspondência entre a altura estimada por medidas segmentares e a estatura real de crianças com paralisia cerebral. Fonte de dados: Revisão sistemática da literatura entre 1995 e 2018, guiada pela diretriz Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), nas bases de dados PubMed, Biblioteca Virtual em Saúde (BVS), Medical Literature Analysis and Retrieval System Online (MEDLINE) e Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS). Os descritores, combinados pelo operador booleano "and", foram: "anthropometry", "cerebral palsy", "child" e "body height". A pesquisa englobou artigos em português, inglês e espanhol, classificadas pelo Quali-CAPES igual ou superior a B3 e que respondiam à questão guia: "Existe correlação entre a altura estimada por equações em crianças com paralisia cerebral e as medidas diretas de altura?". Dos 152 artigos inicialmente recuperados, sete foram selecionados e sua qualidade metodológica foi avaliada pela escala da Agency for Healthcare Research and Quality (AHRQ). Síntese dos dados: A maioria dos trabalhos não encontrou correspondência entre altura real e estimada. Estudos que exibiram coincidência das medidas apresentaram limitações que poderiam comprometer os resultados (perda de amostra, amostra pequena e exclusão de indivíduos com contraturas severas, escoliose e paralisia cerebral grave). Pesquisadores japoneses desenvolveram equação que apresenta boa concordância entre as estaturas. Contudo, o estudo compreendeu apenas indivíduos japoneses. Conclusões: Dada a importância da precisão das medidas de estatura para avaliar a saúde infantil, tornam-se necessárias mais pesquisas visando estabelecer, de maneira mais segura, a associação entre a estatura estimada e a real. O desenvolvimento de protocolos antropométricos, resultantes dessas pesquisas, beneficiaria o acompanhamento de crianças com sequelas psicomotoras graves.

The Structure and Mute Zone of Social Representations Made by Members of the Municipal Council for the Rights of the Elderly / Estrutura e Zona Muda das Representações Sociais de Conselheiros Municipais do Idoso / Estructura y Zona Muda de Representaciones Sociales de Consejeros Municipales del Anciano

Abstract The Municipal Council of the Elderly is the body established by law to watch over the rights of the elderly and it is up to them to submit, opine, discuss, debate and decide, by vote, issues related to the rights of the elderly. The objective of this research is to identify and analyze how the social representations of counselors about the elderly municipal council are organized and the possible elements that make up the mute zone. A total of 72 counselors participated in the survey, who answered a sociodemographic questionnaire and a hierarchical free speech evocation test in situations of normal collection and substitution, based on the inductive term "municipal council of the elderly". The data were analyzed by the Prototypic Analysis and the Centrality Index of Social Representations from Evocations. The results showed that although the council is normatively represented as a body for the defense and guarantee of the rights of the elderly, elements of the mute zone in the central nucleus point it, against normatively, as an inefficient, unknown body of denunciation, which needs better planning and reorganization. It was considered that all social representation is masked, requiring further research on the subject.

Resumo O Conselho Municipal do Idoso é o órgão de direito instituído para zelar pelos direitos dos idosos e cabe a eles, submeter, opinar, discutir, debater e decidir, por meio de voto, assuntos relacionados aos direitos do idoso. O objetivo desta pesquisa é identificar e analisar como se organizam as representações sociais de conselheiros sobre o conselho municipal do idoso e os possíveis elementos que compõem a zona muda. Participaram da pesquisa 72 conselheiros, que responderam questionário sociodemográfico e Teste de Evocação Livre de Palavras hierarquizada em situações de coleta normal e substituição, a partir do termo indutor "conselho municipal do idoso". Os dados foram analisados pela Análise Prototípica e pelo Índice de Centralidade de Representações Sociais a partir de Evocações. Os resultados apontaram que apesar do conselho ser representado normativamente como órgão de defesa e garantia dos direitos dos idosos, elementos da zona muda no núcleo central o aponta, contra normativamente, como órgão de denúncias, ineficiente, desconhecido, que precisa melhor planejamento e reorganização. Considerou-se, que toda representação social encontra-se mascarada, havendo necessidade de novas pesquisas sobre a temática.

Resumen Consejo Municipal del Anciano es órgano de derecho instituido para velar por derechos de ancianos, e que corresponde a ellos, someter, opinar, discutir, debatir y decidir, por medio de voto, asuntos relacionados los derechos del anciano. El objetivo de esta investigación es identificar y analizar cómo se organizan las representaciones sociales de consejeros sobre el consejo municipal del anciano y los posibles elementos que componen la zona muda. Participaron, 72 consejeros, que respondieron cuestionario sociodemográfico y Test Evocación Libre de Palabras jerarquizada en situaciones de recolección de normal y de sustitución, a partir del término inductor "consejo municipal del ancianos". Datos fueron analizados por Análisis Prototípico y por Índice de Centralidad de Representaciones Sociales a partir de Evocaciones. Resultados apuntaron que apesar de que consejo es representado normativamente como órgano de defensa y garantía de derechos de los ancianos, elementos de la zona muda en el núcleo central lo apunta, contra normativamente, como órgano de denuncias, ineficiente, desconocido, que necesita mejor planificación y reorganización. Se consideró, que toda representación social se encuentra enmascarada, habiendo necesidad de nuevas investigaciones sobre la temática.

Influence of sediment parameters on the distribution and fate of PAHs in an estuarine tropical region located in the Brazilian semi-arid (Jaguaribe River, Ceará coast).

18 polycyclic aromatic hydrocarbons (PAHs) were evaluated in the Jaguaribe River to explore the influence of grain size, organic carbon, humic and fulvic acids and black carbon on their adsorption onto sediment. The ∑PAHs concentrations variated from 0.6 to 3752.0 ng g-1 with highest concentrations in the estuarine zone. The PAHs predominant source along the river was from mixed sources, mainly related to biomass combustion, small oil spills related to recreational nautical activities and runoff from cities. Organic and inorganic parameters presented influence on PAHs distribution along the river, with humic acid as a determinant factor. These research findings are of importance to an assessment of the fate and transport of PAHs in estuarine systems.

Diagnóstico parasitológico de Tritrichomonas muris (Grassi, 1879) em hamster golden (Mesocricetus auratus) (Waterhouse, 1839): comparação entre as técnicas de sedimentação, flutuação e exame direto / Parasitological diagnosis of Tritrichomonas muris (Grassi, 1879) in golden hamster (Mesocricetus auratus) (Waterhouse, 1839): comparison between sedimentation, flotation and direct examination techniques

A experimentação animal é primordial para os avanços científicos em prol da saúde humana e animal. Inúmeros parasitos que acometem animais de laboratório podem interferir nas pesquisas, comprometendo os resultados. O protozoário Tritrichomonas muris pode ser um bioindicador de quebra de barreiras sanitárias em instalações de animais de laboratório, auxiliando na conduta do manejo e melhoria do padrão de qualidade animal. Este trabalho comparou três métodos parasitológicos, visando implantar a metodologia mais adequada em uma rotina de monitoramento sanitário de um biotério de criação de hamsters golden (Mesocricetus auratus). Foram utilizados 50 hamsters, entre três meses a um ano de idade, selecionados por amostragem, para exames parasitológicos nos períodos de monitoramento sanitário pré-estabelecidos. A análise foi feita por dois anos, abrangendo um total de oito exames trimestrais. O diagnóstico foi realizado por exame direto, flutuação de Willis-Mollay e sedimentação espontânea de Dennis Stone & Swanson a partir da mucosa intestinal e das fezes frescas in natura. Foram identificados apenas trofozoítos de T. muris nas três primeiras porções intestinais selecionadas no estudo (intestino delgado, ceco e cólon), enquantoa forma de pseudocisto foi detectada nas amostras fecais e na região do reto onde já há fezes formadas. Tais achados são embasados no mecanismo do ciclo de vida do protozoário em questão. O método direto mostrou maior eficácia para o diagnóstico de T. muris para a identificação de trofozoítos e pseudocistos, tanto nas fezes formadas, como na mucosa intestinal devidamente fragmentada em suas diferentes regiões.(AU)

Animal experimentation is primordial to scientific advances for human and animal health. Numerous parasites that affect laboratory animals can interfere with research, compromising the results. The protozoan Tritrichomonas muris can be a bioindicator of breaking down health barriers in laboratory animal facilities, helping to conduct management and improve animal quality standards. This work compared three parasitological methods, aiming to implement the most suitable methodology in a sanitary monitoring routine of a golden hamster breeding (Mesocricetus auratus). Fifty hamsters from three months to one year of age, selected by sampling, were used for parasitological examinations in pre-established sanitary monitoring periods. Analysis was performed for two years, covering a total of eight quarterly exams. The diagnosis was carried out by direct examination, Willis-Mollay flotation and spontaneous sedimentation by Dennis Stone & Swanson from the intestinal mucosa and fresh stool. Only T. muris trophozoites were identified in the first three intestinal portions selected in the study (small intestine, cecum and colon), while the pseudocyst form was detected in faecal samples and in the rectal region where there are already formed stools. Such findings are based on the life-cycle mechanism of this protozoan. The direct method showed greater efficacy for the diagnosis of T. muris for the identification of trophozoites and pseudocysts, both in formed feces and in the intestinal mucosa, properly fragmented in their different regions.(AU)

Diagnóstico parasitológico de Tritrichomonas muris (Grassi, 1879) em hamster golden (Mesocricetus auratus) (Waterhouse, 1839): comparação entre as técnicas de sedimentação, flutuação e exame direto / Parasitological diagnosis of Tritrichomonas muris (Grassi, 1879) in golden hamster (Mesocricetus auratus) (Waterhouse, 1839): comparison between sedimentation, flotation and direct examination techniques

A experimentação animal é primordial para os avanços científicos em prol da saúde humana e animal. Inúmeros parasitos que acometem animais de laboratório podem interferir nas pesquisas, comprometendo os resultados. O protozoário Tritrichomonas muris pode ser um bioindicador de quebra de barreiras sanitárias em instalações de animais de laboratório, auxiliando na conduta do manejo e melhoria do padrão de qualidade animal. Este trabalho comparou três métodos parasitológicos, visando implantar a metodologia mais adequada em uma rotina de monitoramento sanitário de um biotério de criação de hamsters golden (Mesocricetus auratus). Foram utilizados 50 hamsters, entre três meses a um ano de idade, selecionados por amostragem, para exames parasitológicos nos períodos de monitoramento sanitário pré-estabelecidos. A análise foi feita por dois anos, abrangendo um total de oito exames trimestrais. O diagnóstico foi realizado por exame direto, flutuação de Willis-Mollay e sedimentação espontânea de Dennis Stone & Swanson a partir da mucosa intestinal e das fezes frescas in natura. Foram identificados apenas trofozoítos de T. muris nas três primeiras porções intestinais selecionadas no estudo (intestino delgado, ceco e cólon), enquantoa forma de pseudocisto foi detectada nas amostras fecais e na região do reto onde já há fezes formadas. Tais achados são embasados no mecanismo do ciclo de vida do protozoário em questão. O método direto mostrou maior eficácia para o diagnóstico de T. muris para a identificação de trofozoítos e pseudocistos, tanto nas fezes formadas, como na mucosa intestinal devidamente fragmentada em suas diferentes regiões.

Animal experimentation is primordial to scientific advances for human and animal health. Numerous parasites that affect laboratory animals can interfere with research, compromising the results. The protozoan Tritrichomonas muris can be a bioindicator of breaking down health barriers in laboratory animal facilities, helping to conduct management and improve animal quality standards. This work compared three parasitological methods, aiming to implement the most suitable methodology in a sanitary monitoring routine of a golden hamster breeding (Mesocricetus auratus). Fifty hamsters from three months to one year of age, selected by sampling, were used for parasitological examinations in pre-established sanitary monitoring periods. Analysis was performed for two years, covering a total of eight quarterly exams. The diagnosis was carried out by direct examination, Willis-Mollay flotation and spontaneous sedimentation by Dennis Stone & Swanson from the intestinal mucosa and fresh stool. Only T. muris trophozoites were identified in the first three intestinal portions selected in the study (small intestine, cecum and colon), while the pseudocyst form was detected in faecal samples and in the rectal region where there are already formed stools. Such findings are based on the life-cycle mechanism of this protozoan. The direct method showed greater efficacy for the diagnosis of T. muris for the identification of trophozoites and pseudocysts, both in formed feces and in the intestinal mucosa, properly fragmented in their different regions.