ABSTRACT
BACKGROUND: This study aimed to compare the IMG counting by an auto hematology analyzer with the flow cytometric enumeration of CD34+ cells. METHODS: All data from 124 samples submitted to the hematology laboratory for CD34+ cell counting in 2019 and 2020 were retrospectively evaluated. Whole blood samples were taken into EDTA tubes and assayed within 2 hours. The numbers and percentages of WBC and IMG were determined by using Mindray BC6200 hematology analyzer, while the same were determined for CD45 and CD34+ cells by using Beckman Coulter Navios flow cytometer. The performance of the new method was determined by the receiver operating characteristic (ROC) analysis. RESULTS: Out of the 124 samples, 94 were collected from healthy individuals and 30 were collected from patients. A significant correlation was found between IMG and CD34+ cell counts in all patients (r = 0.71, p = 0.000) at the cutoff values of > 20/µL and > 50/µL. The Bland-Altman analysis of all patients showed that there was an agreement between the two methods. When the cutoff value of 20/µL for CD34+ cells was used in the ROC analysis, the sensitivity and specificity were calculated as 100 (96.1 - 100) and 96.77 (83.3 - 99.9), respectively, for the IMG count of > 900/µL. CONCLUSIONS: An IMG count of 900/µL can be used as the cutoff value in the analysis with the Mindray BC6200. The IMG counting cannot replace the flow cytometric CD34+ cell enumeration but can be used in the selection of the samples for stem cell enumeration by flow cytometry.
Subject(s)
Antigens, CD34 , Flow Cytometry , Humans , Flow Cytometry/methods , Antigens, CD34/blood , Retrospective Studies , Female , Male , Adult , Middle Aged , Leukocyte Count/methods , ROC Curve , Young Adult , Aged , Granulocyte Precursor Cells/cytology , Granulocytes/cytology , Sensitivity and SpecificityABSTRACT
OBJECTIVE: To evaluate idiopathic hirsutism etiology via molecular studies testing peripheral and local aromatase and 5α-reductase expression. DESIGN: Assessment of the expression of messenger RNA (mRNA) for type 1 and 2,5α-reductase isoenzyme gene (SDR5A1, SDR5A2) and aromatase (CYP19A) in dermal papillae cells and peripheral blood mononuclear cells. SETTING: University hospital. PATIENT(S): 28 untreated idiopathic hirsute patients and 20 healthy women (controls). INTERVENTION(S): Human skin biopsies and peripheral venous blood. MAIN OUTCOME MEASURE(S): SDR5A1, SDR5A2, CYP19A gene expression in skin biopsies and peripheral blood. RESULT(S): A statistically significant reduction of SRD5A1, SRD5A2, and CYP19A gene expression was found in the dermal papillae cells and peripheral blood mononuclear cell between the study and control group. CONCLUSION(S): Further study, including protein expression and enzyme activity assays, are warranted to characterize the paradoxically low gene expression levels of local 5α-reductase and aromatase in women with idiopathic hirsutism.