Glyphosate in oat desiccation for haylage harvest at different wilting times

The objective was to evaluate the ruminal disappearance rate of dry matter and NDF of black oat haylage subjected to mechanical and chemical dehydration and six wilting periods in the field, as follows: 0, 3, 6, 9, 12 and 15 days. Forage haylageswere ensiled and stored in PVC mini-silos for 60 days until opening for in situ evaluations. The pre-set ruminal incubation times were: 0, 6, 12, 24, 36 and 48 hours. Regardless of the dehydration time, forage subjected to mechanical dehydration showed ruminal disappearance rates of dry matter and NDF, thus DISDM-48h and DISNDF-48h were superior for this method compared to the chemical method. There was a reduction in DISDM-48h and DISNDF-48h with advancing time of wilting in the field for haylage dehydrated by the chemical method. In general, the chemical dehydration method seems to be more related to better haylage making practices than nutritional factors, where the haylage obtained by mechanical dehydration is superior.(AU)

Effects of Obligate Heterofermentative Lactic Acid Bacteria Alone or in Combination on the Conservation of Sugarcane Silage.

Our objective was to determine the effects of two strains of obligate heterofermentative bacteria, alone or in combination, on the fermentation profile, gas production kinetics, chemical composition, and aerobic stability of sugarcane silage. A plot of sugarcane was manually harvested, mechanically chopped and treated with: distilled water (5 mL kg-1; Control), Lentilactobacillus hilgardii CNCM I-4785 [3 × 105 colony-forming units (cfu) g-1; LH], Lentilactobacillus buchneri NCIMB 40788 (3 × 105 cfu g-1; LB), and LH+LB (1.5 × 105 cfu g-1 of each strain). Treated forages were packed into 1.96-L gas-tight silos (0.40 porosity) and stored at 25 ± 1.5°C for 70 days (4 replicates per treatment). All heterolactic inoculants were effective to increase acetic acid concentration and inhibit yeast metabolism, as treated silages had lower formation of ethanol, ethyl esters and gas during fermentation. Lower fungal development spared soluble carbohydrates, consequently resulting in silages with higher in vitro digestibility. Nevertheless, L. buchneri was the most effective strain to extend the aerobic stability of sugarcane silage (based on both temperature and pH rise). The use of L. buchneri alone or in combination with L. hilgardii, applied at 3 × 105 cfu g-1, is a feasible strategy to inhibit yeast metabolism and increase the nutritional quality of sugarcane silage.

Evaluation of rehydrated corn grain silage with okara or soybean inclusion in the performance of lactating cows / Avaliação da silagem de grãos de milho reidratados com inclusão de okara ou soja no desempenho de vacas em lactação

This study aimed to evaluate dry matter intake, nutrient digestibility, milk production and composition, and feed efficiency of cows fed corn grain silages with okara or raw soybean inclusion. Six Holstein cows were distributed in a double Latin square and arranged in the following treatments: concentrate formulated based on corn grain silage with 30% okara inclusion; concentrate of corn grain silage with 20% raw soybean inclusion, and concentrate based on dry corn grains. Statistical analysis was performed using Bayesian inference. Diets formulated with silages reduced dry matter intake compared to the diet of concentrate based on dry grains. High digestibility of DM, OM, NFC, and EE was observed for diets with silage inclusion. Milk presented an increase in CLA for the diet of corn grain silage diet with soybean inclusion. Rehydrated grain corn silage with soybean or okara inclusion reduced dry matter intake and increased nutrient digestibility, maintaining the same milk production, which demonstrates the higher efficiency of these diets.(AU)

Objetivou-se avaliar a ingestão de matéria seca, digestibilidade dos nutrientes, produção e composição do leite e a eficiência alimentar de vacas alimentadas com silagens de grãos de milho com adição de okara ou de soja crua. Foram utilizadas 6 vacas da raça Holandesa, distribuídas em um duplo quadrado latino, nos seguintes tratamentos: concentrado formulado à base de silagem de grãos de milho com adição de 30% de okara; concentrado com silagem de grãos de milho com adição de 20% de soja crua e concentrado à base de grãos secos de milho. A análise estatística foi realizada por meio de Inferência Bayesiana. As dietas formuladas com as silagens diminuíram o consumo de matéria seca em relação à dieta com concentrado à base de grãos secos. Foi verificada maior digestibilidade da MS, MO, CNF e EE para as dietas com a inclusão das silagens. Houve aumento do CLA no leite para a dieta com silagem de grãos de milho com adição de soja. A ensilagem de grãos de milho reidratados com adição de soja ou okara diminui o consumo de matéria seca, aumenta a digestibilidade dos nutrientes, mantendo a mesma produção de leite, o que demonstra superioridade na eficiência destas dietas.(AU)

Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus.

Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). BLIS inhibited the growth of spoilage bacteria belonging to Lactobacillus, Enterococcus and Listeria genera with higher activity than Nisaplin used as control. It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50-20.00 mm). It also effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. These results point out that P. pentosaceus BLIS may be used as a new promising alternative to conventional antibacterial and antifungal substances, with potential applications in agriculture and food industry as a natural bio-controlling agent. Moreover, cytotoxicity and cell death induction tests demonstrated cytotoxicity and toxicity of BLIS to human colon adenocarcinoma Caco-2cells but not to peripheral blood mononuclear cells, with suggests possible applications of BLIS also in medical-pharmaceutical applications.

Chemical composition, aerobic stability, and fermentation pattern of white oat silage wilted with glyphosate / Composição química, estabilidade aeróbia e padrão fermentativo de silagens de aveia branca dessecadas com glifosato

White oat has good nutritional quality but is not an easy forage to ensile due to its high buffer capacity and moisture content at ensiling moment. Therefore, wilting is necessary to offset such negative aspects. However, this process demands skilled workforce and adequate machinery. In this way, chemical desiccation is a promising technology to reduce the steps needed for wilting. Thus, we aimed to evaluate the effects of glyphosate as a chemical desiccant on the nutritional quality, fermentation pattern, losses, and aerobic stability of wilted white oat (Avena sativa) silages. White oat sowing occurred in the first fortnight of May 2013. Desiccant application took place when oat reached milky-dough grain stage (96 days after planting). Glyphosate doses evaluated were 0, 500, 750, 1000, and 1250 mL ha-1. Three days after desiccation, all treatments were ensiled, and the silos were stored for 150 days. A completely randomized design was used, and all statistical procedures were performed by means of Bayesian Inference. No differences were found for lactic acid, but treated-silage pH linearly decreased. The lowest concentration of butyric acid (3.40 mg kg-1) was observed at 900.80 mL ha-1. For ammonia, the highest point (50 g kg-1) occurred at 916.51 mL ha-1. Aerobic stability was not influenced by treatments. Maximum dry matter recovery index (934 g kg-1) was observed at 864.20mL ha-1 glyphosate. Wilted forage from treatments 500 mL ha-1, 750 mL ha-1, and 1000 mL ha-1 had greater dry matter content compared to control (320.1, 326, 301.3, and 270.7 g kg-1 respectively). Hemicellulose linearly decreased and crude protein linearly increased. The lowest concentrations of neutral detergent fiber (642.8 g kg-1) and neutral-detergent insoluble nitrogen (2.30 g kg-1) occurred at doses of 1141.32 mL ha-1 and 829.14 mL ha-1, respectively. In brief, for wilted white oat silage production, harvested at milky-dough grain stage, glyphosate application...

A aveia branca (Avena sativa) apresenta boa qualidade nutricional, contudo, devido à sua alta capacidade tampão e elevado teor de umidade no momento do corte, acaba por dificultar o processo de conservação como silagem. Dessa forma, a pré-secagem é necessária para compensar os aspectos negativos relacionados à ensilagem da aveia. No entanto, a pré-secagem exige mão-de-obra qualificada bem como maquinário específico. Desta forma, a dessecação química é uma tecnologia promissora, reduzindo as etapas necessárias para a pré-secagem, facilitando o processo. Sendo assim, objetivou-se foi avaliar os efeitos do uso do glifosato na pré-secagem da avia branca sobre a qualidade nutricional, padrão de fermentativo e perdas bem como na estabilidade aeróbia das silagens resultantes. A semeadura de aveia branca ocorreu na primeira quinzena de maio de 2013. A aplicação do dessecante ocorreu quando a aveia atingiu o estágio de grãos leitoso/pastoso (96 dias após o plantio). As doses de glifosato avaliadas foram 0, 500, 750, 1000 e 1250 mL ha-1. Três dias após a dessecação, todos os tratamentos foram ensilados e os silos foram armazenados por 150 dias. O delineamento experimental foi o inteiramente casualizado e todos os procedimentos estatísticos foram realizados por meio da inferência bayesiana. Não foram encontradas diferenças para as concentrações de ácido láctico, no entanto, o pH diminuiu linearmente nas silagens tratadas. A menor concentração de ácido butírico (3,40 mg kg-1) foi observada para a dosem de 900,80 mL ha-1. A maior concentração de amônia (50 g kg-1) foi encontrada na dosem de 916,51 mL ha-1. A estabilidade aeróbica não foi influenciada pelos tratamentos neste estudo. O maior índice de recuperação de matéria seca (934 g kg-1) foi observado para a dosem de 864,20 mL ha-1 de glifosato. As silagens provenientes dos tratamentos 500 mL ha-1, 750 mL ha-1 e 1000 mL ha-1 apresentaram...

Chemical composition, aerobic stability, and fermentation pattern of white oat silage wilted with glyphosate / Composição química, estabilidade aeróbia e padrão fermentativo de silagens de aveia branca dessecadas com glifosato

White oat has good nutritional quality but is not an easy forage to ensile due to its high buffer capacity and moisture content at ensiling moment. Therefore, wilting is necessary to offset such negative aspects. However, this process demands skilled workforce and adequate machinery. In this way, chemical desiccation is a promising technology to reduce the steps needed for wilting. Thus, we aimed to evaluate the effects of glyphosate as a chemical desiccant on the nutritional quality, fermentation pattern, losses, and aerobic stability of wilted white oat (Avena sativa) silages. White oat sowing occurred in the first fortnight of May 2013. Desiccant application took place when oat reached milky-dough grain stage (96 days after planting). Glyphosate doses evaluated were 0, 500, 750, 1000, and 1250 mL ha-1. Three days after desiccation, all treatments were ensiled, and the silos were stored for 150 days. A completely randomized design was used, and all statistical procedures were performed by means of Bayesian Inference. No differences were found for lactic acid, but treated-silage pH linearly decreased. The lowest concentration of butyric acid (3.40 mg kg-1) was observed at 900.80 mL ha-1. For ammonia, the highest point (50 g kg-1) occurred at 916.51 mL ha-1. Aerobic stability was not influenced by treatments. Maximum dry matter recovery index (934 g kg-1) was observed at 864.20mL ha-1 glyphosate. Wilted forage from treatments 500 mL ha-1, 750 mL ha-1, and 1000 mL ha-1 had greater dry matter content compared to control (320.1, 326, 301.3, and 270.7 g kg-1 respectively). Hemicellulose linearly decreased and crude protein linearly increased. The lowest concentrations of neutral detergent fiber (642.8 g kg-1) and neutral-detergent insoluble nitrogen (2.30 g kg-1) occurred at doses of 1141.32 mL ha-1 and 829.14 mL ha-1, respectively. In brief, for wilted white oat silage production, harvested at milky-dough grain stage, glyphosate application...(AU)

A aveia branca (Avena sativa) apresenta boa qualidade nutricional, contudo, devido à sua alta capacidade tampão e elevado teor de umidade no momento do corte, acaba por dificultar o processo de conservação como silagem. Dessa forma, a pré-secagem é necessária para compensar os aspectos negativos relacionados à ensilagem da aveia. No entanto, a pré-secagem exige mão-de-obra qualificada bem como maquinário específico. Desta forma, a dessecação química é uma tecnologia promissora, reduzindo as etapas necessárias para a pré-secagem, facilitando o processo. Sendo assim, objetivou-se foi avaliar os efeitos do uso do glifosato na pré-secagem da avia branca sobre a qualidade nutricional, padrão de fermentativo e perdas bem como na estabilidade aeróbia das silagens resultantes. A semeadura de aveia branca ocorreu na primeira quinzena de maio de 2013. A aplicação do dessecante ocorreu quando a aveia atingiu o estágio de grãos leitoso/pastoso (96 dias após o plantio). As doses de glifosato avaliadas foram 0, 500, 750, 1000 e 1250 mL ha-1. Três dias após a dessecação, todos os tratamentos foram ensilados e os silos foram armazenados por 150 dias. O delineamento experimental foi o inteiramente casualizado e todos os procedimentos estatísticos foram realizados por meio da inferência bayesiana. Não foram encontradas diferenças para as concentrações de ácido láctico, no entanto, o pH diminuiu linearmente nas silagens tratadas. A menor concentração de ácido butírico (3,40 mg kg-1) foi observada para a dosem de 900,80 mL ha-1. A maior concentração de amônia (50 g kg-1) foi encontrada na dosem de 916,51 mL ha-1. A estabilidade aeróbica não foi influenciada pelos tratamentos neste estudo. O maior índice de recuperação de matéria seca (934 g kg-1) foi observado para a dosem de 864,20 mL ha-1 de glifosato. As silagens provenientes dos tratamentos 500 mL ha-1, 750 mL ha-1 e 1000 mL ha-1 apresentaram...(AU)

Nutritional value and digestible dry matter production of oat genotypes for ensiling / Valor nutricional e produção de matéria seca digestível de cultivares de aveia visando à produção de silagem

This study evaluated dry matter yield and nutritional characteristics of different oat genotypes (Avena spp.) for ensiling. Treatments consisted of genotypes of white oat IPR 126 (Avena sativa), black oat Cabocla IPR and Agrocoxilha (Avena strigosa), and BRS Madrugada and BRS Centauro (Avena vertis). Oats were harvested at the phenological stage of milk/dough grain. The design was a completely randomized block scheme, with five treatments and three replications per treatment. The IPR Cabocla genotype showed the highest dry matter content (before and after silo opening) and in vitro dry matter digestibility of fresh forage. No differences were observed for in vitro dry matter digestibility and dry matter losses among silages. The highest silage digestible dry matter yield (kg ha-1) was observed for the BRS Centauro genotype. Thus, despite the better nutritional quality presented by the IPR Cabocla genotype before ensiling, BRS Centauro genotype presented a higher yield of digestible dry matter per hectare.(AU)

Este trabalho teve como objetivo avaliar as características produtivas e nutricionais de diferentes cultivares de aveia (Avena spp.),visando à produção de silagem. Os tratamentos foram constituídos dos cultivares de aveia branca IPR 126 (Avena sativa), Agrocoxilha, e IPR Cabocla (Avena strigosa), bem como das cultivares BRS Madrugada e BRS Centauro (Avena vertis). As forragens foram colhidas e ensiladas quando atingiram o estádio fenológico de grão pastoso/farináceo. O delineamento experimental foi em blocos casualizados, com cinco tratamentos e três repetições por tratamento. O cultivar Cabocla apresentou os maiores teores de matéria seca (antes e após a abertura dos silos) e maior digestibilidade in vitro da matéria seca no momento de ensilagem. Após a ensilagem não foram observadas diferenças para a digestibilidade in vitro e perdas de matéria seca entre os tratamentos. Contudo, após correção dos respectivos valores para perdas de matéria seca na ensilagem e digestibilidade in vitro da matéria seca, observou-se maior produção de matéria seca digestível (kg ha-1) para o cultivar Centauro.(AU)

Nutritional value and digestible dry matter production of oat genotypes for ensiling / Valor nutricional e produção de matéria seca digestível de cultivares de aveia visando à produção de silagem

This study evaluated dry matter yield and nutritional characteristics of different oat genotypes (Avena spp.) for ensiling. Treatments consisted of genotypes of white oat IPR 126 (Avena sativa), black oat Cabocla IPR and Agrocoxilha (Avena strigosa), and BRS Madrugada and BRS Centauro (Avena vertis). Oats were harvested at the phenological stage of milk/dough grain. The design was a completely randomized block scheme, with five treatments and three replications per treatment. The IPR Cabocla genotype showed the highest dry matter content (before and after silo opening) and in vitro dry matter digestibility of fresh forage. No differences were observed for in vitro dry matter digestibility and dry matter losses among silages. The highest silage digestible dry matter yield (kg ha-1) was observed for the BRS Centauro genotype. Thus, despite the better nutritional quality presented by the IPR Cabocla genotype before ensiling, BRS Centauro genotype presented a higher yield of digestible dry matter per hectare.

Este trabalho teve como objetivo avaliar as características produtivas e nutricionais de diferentes cultivares de aveia (Avena spp.),visando à produção de silagem. Os tratamentos foram constituídos dos cultivares de aveia branca IPR 126 (Avena sativa), Agrocoxilha, e IPR Cabocla (Avena strigosa), bem como das cultivares BRS Madrugada e BRS Centauro (Avena vertis). As forragens foram colhidas e ensiladas quando atingiram o estádio fenológico de grão pastoso/farináceo. O delineamento experimental foi em blocos casualizados, com cinco tratamentos e três repetições por tratamento. O cultivar Cabocla apresentou os maiores teores de matéria seca (antes e após a abertura dos silos) e maior digestibilidade in vitro da matéria seca no momento de ensilagem. Após a ensilagem não foram observadas diferenças para a digestibilidade in vitro e perdas de matéria seca entre os tratamentos. Contudo, após correção dos respectivos valores para perdas de matéria seca na ensilagem e digestibilidade in vitro da matéria seca, observou-se maior produção de matéria seca digestível (kg ha-1) para o cultivar Centauro.

Evaluation of rehydrated corn grain silage with okara or soybean inclusion in the performance of lactating cows / Avaliação da silagem de grãos de milho reidratados com inclusão de okara ou soja no desempenho de vacas em lactação

This study aimed to evaluate dry matter intake, nutrient digestibility, milk production and composition, and feed efficiency of cows fed corn grain silages with okara or raw soybean inclusion. Six Holstein cows were distributed in a double Latin square and arranged in the following treatments: concentrate formulated based on corn grain silage with 30% okara inclusion; concentrate of corn grain silage with 20% raw soybean inclusion, and concentrate based on dry corn grains. Statistical analysis was performed using Bayesian inference. Diets formulated with silages reduced dry matter intake compared to the diet of concentrate based on dry grains. High digestibility of DM, OM, NFC, and EE was observed for diets with silage inclusion. Milk presented an increase in CLA for the diet of corn grain silage diet with soybean inclusion. Rehydrated grain corn silage with soybean or okara inclusion reduced dry matter intake and increased nutrient digestibility, maintaining the same milk production, which demonstrates the higher efficiency of these diets.

Objetivou-se avaliar a ingestão de matéria seca, digestibilidade dos nutrientes, produção e composição do leite e a eficiência alimentar de vacas alimentadas com silagens de grãos de milho com adição de okara ou de soja crua. Foram utilizadas 6 vacas da raça Holandesa, distribuídas em um duplo quadrado latino, nos seguintes tratamentos: concentrado formulado à base de silagem de grãos de milho com adição de 30% de okara; concentrado com silagem de grãos de milho com adição de 20% de soja crua e concentrado à base de grãos secos de milho. A análise estatística foi realizada por meio de Inferência Bayesiana. As dietas formuladas com as silagens diminuíram o consumo de matéria seca em relação à dieta com concentrado à base de grãos secos. Foi verificada maior digestibilidade da MS, MO, CNF e EE para as dietas com a inclusão das silagens. Houve aumento do CLA no leite para a dieta com silagem de grãos de milho com adição de soja. A ensilagem de grãos de milho reidratados com adição de soja ou okara diminui o consumo de matéria seca, aumenta a digestibilidade dos nutrientes, mantendo a mesma produção de leite, o que demonstra superioridade na eficiência destas dietas.

Natamycin as a potential silage additive: A lab trial using sugarcane to assess greenhouse gas emissions

The objective of this study was to evaluate natamycin, Lactobacillus buchneri (LB), or their combination on the chemical composition, loss, fermentative profile, and aerobic stability as well as gas production and composition of sugarcane silages. The treatments were (wet basis): no additive (control), 10 g t−1 of natamycin (N10), 5 × 104 cfu g−1 of LB, and the combination of 4 g t−1 of natamycin and 2.5 × 104 cfu g−1 of LB (NLB). Sugarcane was chopped (10 mm), treated with the additives, and ensiled in experimental silos (four replicates). The silos remained stored for 51 days. The LB inoculation, alone or in combination with natamycin, increased the acetic acid content (by 105 and 78% respectively) and decreased ethanol content (by 83 and 71% respectively) when compared to N10 treatment and the control. A decrease in both dry matter and gas losses was observed in the LB (by 72 and 78%, respectively) and N10 (by 69 and 77%, respectively) silages compared with the control, but not the combination. The N10 treatment reduced greenhouse gas (GHG) emission by 86% compared with the control silage. Control and N10 silages deteriorated to the same extent with aerobic exposure, whereas LB and NLB presented higher aerobic stability. The use of natamycin alone is not recommended when ethanol and aerobic stability are concerns. However, natamycin may be considered for the composition of blend additives to decrease greenhouse gas emission and fermentative loss in silages. Further studies must be carried out to optimize doses of natamycin in blend additives.(AU)

Storage length interacts with maturity to affect nutrient availability in unprocessed flint corn silage

Our objective was to determine the effects of maturity and storage length on the fermentation pattern, ruminal disappearance of nutrients, and recovery of digestible nutrients in flint corn (Zea mays L.) silage harvested with a pull-type machine without a kernel processor. Corn plants were harvested at 300 g kg−1 DM (low dry matter, LDM) or 400 g kg−1 DM (high dry matter, HDM) and stored for 0, 7, 14, 30, 60, 90, 120, and 180 d in laboratory silos. Corn harvested at HDM had a higher proportion of long particles (>19 mm), more intact kernels, and lower kernel processing score. There was an interaction between maturity and storage length for several fermentation end-products, DM recovery, ruminal disappearance of nutrients, and recovery of digestible DM. Overall, the concentrations of fermentation endproducts were higher in LDM than in HDM silage. The DM recovery decreased over time, mainly for LDM silage. Ruminal disappearance of nutrients (starch, CP, and DM) increased with storage length, with greater values for LDM silage at shorter storage, but closer values among LDM and HDM at longer storage periods. The recovery of digestible DM (DM recovery × ruminal disappearance) slightly increased in LDM silage up to 26 d of fermentation, but it markedly increased in HDM silage, mainly up to 60 d of storage. Storing for longer periods is a strategy to partially offset the negative impact of maturity on the digestibility of flint corn silage harvested with pull-type machines without kernel processor.(AU)

Effect of okara levels on corn grain silage

We ensiled different levels of okara and ground corn to evaluate the effects on the fermentative pattern, aerobic stability, and chemical composition of resulting silages. The experimental design was completely randomized with four replicates per treatment. The okara levels were (dry matter basis): control (without okara) and 200, 300, 400, and 500 g kg−1 okara, with four replicates per treatment. Control silage did not contain okara, but water was added to adjust the moisture content (400 g kg−1 as fed). Mixtures were ensiled in lab-scale silos and stored for 150 days. Compared with the control silage, okara inclusion linearly increased crude protein (from 89.1 to 251 g kg−1 DM), ether extract (from 39.6 to 136 g kg−1 DM), neutral detergent fiber (from 79.9 to 174 g kg−1 DM), acid detergent fiber (from 22.4 to 119 g kg−1 DM), and ash (from 12.2 to 32.4 g kg−1 DM), whereas decreased dry matter content and in vitro dry matter digestibility (from 830 to 730 g kg−1 DM). The use of okara linearly increased lactic acid concentration but also intensified secondary fermentation. On the other hand, aerobic stability of silages increased due to okara inclusion because of the higher amount of short-chain fatty acids, such as butyric and acetic acids, which accumulated during fermentation. Okara inclusion in corn grain silage must be conditioned to the dry matter content at ensiling, but must not exceed 200 g kg−1 on dry matter basis.(AU)

Nutritional composition and aerobic stability of wheat and corn silages stored under different environmental conditions / Composição nutricional e estabilidade aeróbia de silagens de trigo e milho armazenadas em diferentes ambientes

We aimed to evaluate nutritional quality, fermentation profile, aerobic stability, and dry matter losses in corn (Zea mays) and wheat (Triticum aestivum "BRS Umbu") silages. Treatments included uninoculated and inoculated (Lactobacillus plantarum and Pediococcus acidilactici, 1.0 × 105 UFC g-1) wheat silage, corn silage from a conventional hybrid and a transgenic hybrid. Nutritional quality and fermentation profile variables were tested in a completely randomized design. Means were compared using Tukeys test at 5% significance. An aerobic stability trial was conducted in a factorial design with two silages (wheat × inoculated wheat; conventional hybrid corn × transgenic hybrid corn) and two temperatures (ambient temperature × controlled temperature at 24C). Data were submitted to ANOVA and means were analyzed by the F test at 5% probability. Inoculation of wheat silage increased dry matter, organic matter, and total carbohydrates, but reduced crude protein by a dilution effect. Regarding the fermentation profile, inoculation reduced acetic acid and butyric acid content, whereas it increased propionic acid in wheat silage. Bt corn hybrid silage showed higher dry matter and lower neutral detergent fiber, whereas transgenic corn silage showed lower content of acetic acid, propionic acid, alcohol, and ammonia. Conversely, Bt hybrid silage showed higher butyric acid...(AU)

Objetivou-se avaliar a qualidade nutricional, perfil fermentativo, assim como a estabilidade aeróbia e perdas de matéria seca das silagens das silagens de milho (Zea mays) e trigo (Triticum aestivum "BRS Umbu"). Os tratamentos foram a silagem de trigo sem inoculante, silagem de trigo com inoculante bacteriano (Lactobacillus plantarum e Pediococcus acidilactici na concentração de 1,0×105 UFC g-1), silagem de milho convencional e silagem de milho transgênico. Para as variaveis relacionadas a qualidade nutricional, assim como perfil fermentativo o delineamento utilizado foi o inteiramente casualizado e as médias foram analisadas pelo teste Tukey ao nível de 5%. Já com relação a estabilidade aeróbia, o delineamento estatístico foi o inteiramente casualizado, em esquema fatorial, sendo 2 silagens (silagem de trigo × silagem de trigo com inoculante; silagem de milho convencional × silagem de milho transgênico) e 2 ambientes em avaliação (temperatura ambiente × temperatura controlada à 24 C). Os dados coletados foram submetidos à análise de variância e as médias foram analisadas pelo teste F ao nível de 5%. A inoculação da silagem de trigo promoveu maior conservação de matéria seca, matéria orgânica e carboidratos totais, levando a redução da concentração de proteína bruta, por efeito de diluição, quando comparada a silagem controle. A silagem de trigo inoculada apresentou os...(AU)

Nutritional composition and aerobic stability of wheat and corn silages stored under different environmental conditions / Composição nutricional e estabilidade aeróbia de silagens de trigo e milho armazenadas em diferentes ambientes

We aimed to evaluate nutritional quality, fermentation profile, aerobic stability, and dry matter losses in corn (Zea mays) and wheat (Triticum aestivum "BRS Umbu") silages. Treatments included uninoculated and inoculated (Lactobacillus plantarum and Pediococcus acidilactici, 1.0 × 105 UFC g-1) wheat silage, corn silage from a conventional hybrid and a transgenic hybrid. Nutritional quality and fermentation profile variables were tested in a completely randomized design. Means were compared using Tukeys test at 5% significance. An aerobic stability trial was conducted in a factorial design with two silages (wheat × inoculated wheat; conventional hybrid corn × transgenic hybrid corn) and two temperatures (ambient temperature × controlled temperature at 24C). Data were submitted to ANOVA and means were analyzed by the F test at 5% probability. Inoculation of wheat silage increased dry matter, organic matter, and total carbohydrates, but reduced crude protein by a dilution effect. Regarding the fermentation profile, inoculation reduced acetic acid and butyric acid content, whereas it increased propionic acid in wheat silage. Bt corn hybrid silage showed higher dry matter and lower neutral detergent fiber, whereas transgenic corn silage showed lower content of acetic acid, propionic acid, alcohol, and ammonia. Conversely, Bt hybrid silage showed higher butyric acid...

Objetivou-se avaliar a qualidade nutricional, perfil fermentativo, assim como a estabilidade aeróbia e perdas de matéria seca das silagens das silagens de milho (Zea mays) e trigo (Triticum aestivum "BRS Umbu"). Os tratamentos foram a silagem de trigo sem inoculante, silagem de trigo com inoculante bacteriano (Lactobacillus plantarum e Pediococcus acidilactici na concentração de 1,0×105 UFC g-1), silagem de milho convencional e silagem de milho transgênico. Para as variaveis relacionadas a qualidade nutricional, assim como perfil fermentativo o delineamento utilizado foi o inteiramente casualizado e as médias foram analisadas pelo teste Tukey ao nível de 5%. Já com relação a estabilidade aeróbia, o delineamento estatístico foi o inteiramente casualizado, em esquema fatorial, sendo 2 silagens (silagem de trigo × silagem de trigo com inoculante; silagem de milho convencional × silagem de milho transgênico) e 2 ambientes em avaliação (temperatura ambiente × temperatura controlada à 24 C). Os dados coletados foram submetidos à análise de variância e as médias foram analisadas pelo teste F ao nível de 5%. A inoculação da silagem de trigo promoveu maior conservação de matéria seca, matéria orgânica e carboidratos totais, levando a redução da concentração de proteína bruta, por efeito de diluição, quando comparada a silagem controle. A silagem de trigo inoculada apresentou os...

Wilting whole crop black oat with glyphosate for ensiling: effects on nutritive, fermentative, and aerobic stability characteristics

We aimed to evaluate the effects of glyphosate as a chemical desiccant on the nutritional quality, fermentation pattern, and aerobic stability of wilted black oat (Avena strigosa Schreb) silage. Black oat sowing occurred in the first fortnight of May 2013. Desiccant application took place when oat reached milky/dough grain stage (96 days after planting). Glyphosate dosages evaluated were 0, 500, 750, 1000, and 1250 mL ha−1. Three days after desiccation, all treatments were ensiled, and the silos were kept stored for 150 days. A completely randomized design was used, and all statistical procedures were performed by means of Bayesian Inference. Treating herbage prior to ensiling from 500 mL ha−1 glyphosate increased dry matter and organic matter contents compared with control. On the other hand, fiber content decreased linearly for desiccated silages, as shown by the negative slopes for neutral detergent fiber, acid detergent fiber, and cellulose. The highest concentrations of hemicellulose and neutral detergent insoluble nitrogen occurred for the dosages of 729.96 mL ha−1 and 759.52 mL ha−1 glyphosate, respectively. Wilted silage had less concentration of acetic acid and isovaleric acid and presented a higher amount of 2,3-butanediol. Due to the lack of beneficial short-chain fatty acids, treated silages had a higher organic matter loss (0.1 g mL−1) and reached a maximum pH (0.009 h mL−1) more quickly than control silage, after aerobic exposure. In this way, for wilted black oat silage production, harvested at milky/dough grain stage, the application of 500 mL ha−1 glyphosate is recommended.(AU)

Production and nutritional value of the wheat silage managed with different cutting systems / Produção e qualidade nutricional da silagem de trigo manejado em diferentes sistemas de corte

Winter cereals are mainly used for human and animal nutrition and several studies are now exploring their potential as conserved forage (hay and silage). Among the winter cereal species available for cultivation in the south of Brazil, which is major winter cereals producer in the country, the wheat cv. BRS Umbu has attracted special attention. However, few studies have investigated the potential of this cultivar for silage production. The aim of this study was to evaluate the production and quality of silage from the dual-purpose wheat, BRS Umbu, subjected to different cut managements treatments: T1 - without cut (control), T2 - one cut and T3 - two cuts. Each plot represented an experimental unit in a randomized block design, with 5 replicates per treatment. Two representative samples were collected from each plot to determine morphological segmentation (stem, leaf and ear) and dry matter (DM) content of the whole plant and its morphological components. At the time of opening of the silos, food chemical analysis and pH determination were performed. Forage mass production decreased by 26.88% and 67.82%, respectively, with one and two cuts, compared to control. The DM content of the ensiled plant was 49.9 g kg-1 for the control, 54.7 g kg-1 with one cut and 63.2 g kg-1 with two cuts, at the time of ensiling. Management cuts changed the morphological components of the plants, with a lower proportion of stem (28%) in plants subjected to two cuts. The control treatment showed fewer leaves in ensiled plant (9.6%) and intermediate amount of stalk (52.8%), and was significantly different (p < 0.05) to treatment of a cut. Regarding feed chemistry evaluations, no significant difference (p > 0.05) was observed for mineral matter (MM), crude protein (CP) and hemicellulose (HEM) between the different cutting regimens. However, neutral detergent fiber (NDF) and acid detergent fiber (ADF) decreased (p < 0.05) as the number of cuts increased...(AU)

O cultivo de cereais de inverno tem como objetivo suprir a demanda alimentar humana e animal, contudo estudos recentes têm focado no seu uso como alimento conservado (feno e silagem). Dentre as várias opções disponíveis para o plantio o trigo cv. BRS Umbu vem despertando interesse. Contudo, poucos estudos tem avaliado o potencial desta cultivar para produção de silagem. Sendo assim, objetivo deste trabalho foi avaliar a produção e a qualidade das silagens obtidas a partir do trigo de duplo propósito, BRS Umbu, manejado em diferentes manejos de cortes: T1 sem corte (controle), T2 um corte e T3 dois cortes. Cada parcela representou uma repetição em um delineamento em blocos casualizados, com cinco repetições por tratamento. Duas amostras representativas de cada parcela foram coletadas para se determinar a composição morfológica e massa seca dos seus componentes. No momento da abertura dos silos, amostras foram coletadas para determinação do pH e composição nutricional. O aumento no número de cortes proporcionou redução na produção de massa de forragem seca na ordem de 26.88% e 67.82% do manejo com um e dois cortes, respectivamente em relação ao controle. No momento da ensilagem o teor de matéria seca (MS) das plantas ensiladas foi de 49,9 para o tratamento sem corte, 54,7 g kg MS-1 para o tratamento com um corte e 63,2 g kg MS-1 para o tratamento com dois cortes. Verificou-se alterações nos componentes morfológicos das plantas, sendo influenciados pelo manejo de cortes, observando-se menor proporção de colmo nas plantas submetidas a dois cortes (28%), porém, apresentando maior proporção de espigas (59,4%), enquanto o tratamento controle mostrou menor quantidade de folhas nas plantas ensiladas (9,6%) e quantidade intermediária de espigas (52,8%), diferindo (P < 0,05) do tratamento de um corte, que apresentou (42,6%) de espigas na planta...(AU)

Cutting height and addition of enzyme-bacterial inoculants on corn silage chemical composition and digestibility in sheep / Altura de corte e adição de inoculante enzimo-bacteriano na composição químico-bromatológica e digestibilidade de silagens de milho avaliada em ovinos

The objective of this experiment was to observe the influence of the height of corn plant cutting, associated or not with an enzyme-bacterial inoculant or not, on the chemical composition and digestibility of the silage in sheep. The cut heights were 20 and 40 centimeters above the soil. The additive inoculant was Maize-All®. We analyzed on silage: pH; dry matter; organic matter; crude protein; ether extract; neutral detergent fiber; acid detergent fiber; hemicellulose; cellulose; lignin; and non-fibrous carbohydrates. At the digestibility trial we evaluated apparent digestibility of DM, OM, CP, EE, NDF, ADF, Hem, Cel and NCF. We observed that a high cut reduced lignin value. The corn silage harvested at 20 cm had 84.0 g. kg-1 DM of lignin, higher value than the corn silage harvested at 40 cm (69.3 g. kg-1 MS), and there was no effect of inoculation. There were no differences among treatments at the digestibility trial. The cutting height reduced just lignin concentration. However, this lignin reduction did not affect digestibility and hence it did not affect the TDN concentration.

Objetivou-se com este ensaio observar a influência da altura de corte da planta de milho, associada ou não a um inoculante enzimo-bacteriano, sobre a composição químico-bromatológica e digestibilidade das silagens avaliadas em ovinos. Os tratamentos foram silagens de milho colhidas a 20 e 40 centímetros acima do nível do solo, com ou sem inoculante. Utilizou-se como inoculante o aditivo Maize-All®. As variáveis analisadas na silagem foram pH, matéria seca, matéria orgânica, proteína bruta, extrato etéreo, fibra em detergente neutro, fibra em detergente ácido, hemicelulose, celulose, lignina e carboidratos não fibrosos. No ensaio de digestibilidade, avaliou-se a digestibilidade aparente da MS, MO, PB, EE, FDN, FDA, Hem e CNF. Observou-se com a elevação da altura de corte redução na concentração de lignina, o milho colhido a 20 cm apresentou uma concentração superior ao colhido a 40 cm, verificando-se concentrações de 84,0 g. kg-1 MS e 69,3 g. kg-1 MS, respectivamente, não havendo efeito da inoculação. Não foi observado efeito significativo dos tratamentos sobre as digestibilidades. A elevação da altura de corte apresentou alteração apenas nas concentrações de lignina. No entanto, essa redução de lignina não influenciou nas digestibilidades e consequentemente não alterou as concentrações de NDT.

Método de obtenção de matéria seca e composição química de volumosos / Dry matter obtainiment method and chemical composition of roughages

Objetivou-se avaliar a influência do método de secagem sobre a composição nutricional de diferentes volumosos. Foram utilizadas quatro gramíneas (Urochloa brizantha cv. Marandu, Urochloa brizantha cv. MG4, Cynodon dactylon cv. Coastcross e Panicum maximum cv. Áries), uma leguminosa forrageira, o estilosante (Stylosanthes cv. Campo Grande) e duas silagens (Zea mays e Triticum aestivum cv. BRS Umbu). Os métodos de secagem se basearam no uso da estufa de ventilação forçada e de forno de micro-ondas. O delineamento estatístico utilizado foi um arranjo fatorial 2 x 8 (2 métodos de secagem e 8 volumosos) com 4 repetições. O método de secagem não influenciou o conteúdo de matéria seca das forragens. A secagem em forno micro-ondas elevou os teores de fibra em detergente neutro nos volumosos avaliados. Houve interação entre o método de secagem e tipo de forragem avaliada, com relação à proteína bruta (estilosante), extrato etéreo (MG4, estilosante e Áries) e nitrogênio insolúvel em detergente neutro (Coastcross, MG4, Marandu e estilosante). O forno de micro-ondas pode ser utilizado como instrumento para obtenção da matéria seca em alimentos volumosos, porém, a técnica pode afetar a composição da forragem avaliada.

It was aimed determinate the influence of drying methods on nutritional composition of different roughages. The experimental material were four grass species (Urochloa brizantha cv. Marandu, Urochloa brizantha cv. MG4, Cynodon dactylon cv. Coastcross e Panicum maximum cv. Áries), one forage legume (Stylosanthes cv. Campo Grande) and two silages (Zea mays e Triticum aestivum cv. BRS Umbu). The drying methods were based on use of forced air oven or microwave oven. The experimental design were a 2 x 8 factorial arrangement (2 drying methods and 8 roughages) with 4 repetitions. There was no effect of drying method on roughages dry matter content. Microwave oven drying increased neutral detergent fiber. There was an interaction between drying method and evaluated roughage. Increased contents of crude protein (estilosante), neutral detergent insoluble nitrogen (Coastcross, MG4, Marandu e estilosante) and ether extract (MG4, estilosante e Áries) were observed when roughages were dried using microwave oven. The microwave oven usage is an alternative to obtain the dry matter content of roughages; however this technic may affect the sample composition.

Production and nutritional value of the wheat silage managed with different cutting systems / Produção e qualidade nutricional da silagem de trigo manejado em diferentes sistemas de corte

Winter cereals are mainly used for human and animal nutrition and several studies are now exploring their potential as conserved forage (hay and silage). Among the winter cereal species available for cultivation in the south of Brazil, which is major winter cereals producer in the country, the wheat cv. BRS Umbu has attracted special attention. However, few studies have investigated the potential of this cultivar for silage production. The aim of this study was to evaluate the production and quality of silage from the dual-purpose wheat, BRS Umbu, subjected to different cut managements treatments: T1 - without cut (control), T2 - one cut and T3 - two cuts. Each plot represented an experimental unit in a randomized block design, with 5 replicates per treatment. Two representative samples were collected from each plot to determine morphological segmentation (stem, leaf and ear) and dry matter (DM) content of the whole plant and its morphological components. At the time of opening of the silos, food chemical analysis and pH determination were performed. Forage mass production decreased by 26.88% and 67.82%, respectively, with one and two cuts, compared to control. The DM content of the ensiled plant was 49.9 g kg-1 for the control, 54.7 g kg-1 with one cut and 63.2 g kg-1 with two cuts, at the time of ensiling. Management cuts changed the morphological components of the plants, with a lower proportion of stem (28%) in plants subjected to two cuts. The control treatment showed fewer leaves in ensiled plant (9.6%) and intermediate amount of stalk (52.8%), and was significantly different (p 0.05) was observed for mineral matter (MM), crude protein (CP) and hemicellulose (HEM) between the different cutting regimens. However, neutral detergent fiber (NDF) and acid detergent fiber (ADF) decreased (p < 0.05) as the number of cuts increased...

O cultivo de cereais de inverno tem como objetivo suprir a demanda alimentar humana e animal, contudo estudos recentes têm focado no seu uso como alimento conservado (feno e silagem). Dentre as várias opções disponíveis para o plantio o trigo cv. BRS Umbu vem despertando interesse. Contudo, poucos estudos tem avaliado o potencial desta cultivar para produção de silagem. Sendo assim, objetivo deste trabalho foi avaliar a produção e a qualidade das silagens obtidas a partir do trigo de duplo propósito, BRS Umbu, manejado em diferentes manejos de cortes: T1 – sem corte (controle), T2 – um corte e T3 – dois cortes. Cada parcela representou uma repetição em um delineamento em blocos casualizados, com cinco repetições por tratamento. Duas amostras representativas de cada parcela foram coletadas para se determinar a composição morfológica e massa seca dos seus componentes. No momento da abertura dos silos, amostras foram coletadas para determinação do pH e composição nutricional. O aumento no número de cortes proporcionou redução na produção de massa de forragem seca na ordem de 26.88% e 67.82% do manejo com um e dois cortes, respectivamente em relação ao controle. No momento da ensilagem o teor de matéria seca (MS) das plantas ensiladas foi de 49,9 para o tratamento sem corte, 54,7 g kg MS-1 para o tratamento com um corte e 63,2 g kg MS-1 para o tratamento com dois cortes. Verificou-se alterações nos componentes morfológicos das plantas, sendo influenciados pelo manejo de cortes, observando-se menor proporção de colmo nas plantas submetidas a dois cortes (28%), porém, apresentando maior proporção de espigas (59,4%), enquanto o tratamento controle mostrou menor quantidade de folhas nas plantas ensiladas (9,6%) e quantidade intermediária de espigas (52,8%), diferindo (P < 0,05) do tratamento de um corte, que apresentou (42,6%) de espigas na planta...

Método de obtenção de matéria seca e composição química de volumosos / Dry matter obtainiment method and chemical composition of roughages

Objetivou-se avaliar a influência do método de secagem sobre a composição nutricional de diferentes volumosos. Foram utilizadas quatro gramíneas (Urochloa brizantha cv. Marandu, Urochloa brizantha cv. MG4, Cynodon dactylon cv. Coastcross e Panicum maximum cv. Áries), uma leguminosa forrageira, o estilosante (Stylosanthes cv. Campo Grande) e duas silagens (Zea mays e Triticum aestivum cv. BRS Umbu). Os métodos de secagem se basearam no uso da estufa de ventilação forçada e de forno de micro-ondas. O delineamento estatístico utilizado foi um arranjo fatorial 2 x 8 (2 métodos de secagem e 8 volumosos) com 4 repetições. O método de secagem não influenciou o conteúdo de matéria seca das forragens. A secagem em forno micro-ondas elevou os teores de fibra em detergente neutro nos volumosos avaliados. Houve interação entre o método de secagem e tipo de forragem avaliada, com relação à proteína bruta (estilosante), extrato etéreo (MG4, estilosante e Áries) e nitrogênio insolúvel em detergente neutro (Coastcross, MG4, Marandu e estilosante). O forno de micro-ondas pode ser utilizado como instrumento para obtenção da matéria seca em alimentos volumosos, porém, a técnica pode afetar a composição da forragem avaliada.(AU)

It was aimed determinate the influence of drying methods on nutritional composition of different roughages. The experimental material were four grass species (Urochloa brizantha cv. Marandu, Urochloa brizantha cv. MG4, Cynodon dactylon cv. Coastcross e Panicum maximum cv. Áries), one forage legume (Stylosanthes cv. Campo Grande) and two silages (Zea mays e Triticum aestivum cv. BRS Umbu). The drying methods were based on use of forced air oven or microwave oven. The experimental design were a 2 x 8 factorial arrangement (2 drying methods and 8 roughages) with 4 repetitions. There was no effect of drying method on roughages dry matter content. Microwave oven drying increased neutral detergent fiber. There was an interaction between drying method and evaluated roughage. Increased contents of crude protein (estilosante), neutral detergent insoluble nitrogen (Coastcross, MG4, Marandu e estilosante) and ether extract (MG4, estilosante e Áries) were observed when roughages were dried using microwave oven. The microwave oven usage is an alternative to obtain the dry matter content of roughages; however this technic may affect the sample composition.(AU)