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1.
Zoonoses Public Health ; 64(6): 423-430, 2017 09.
Article in English | MEDLINE | ID: mdl-27863049

ABSTRACT

In Brazil, rabies control in dogs and cats was pioneered by the state of São Paulo with the adoption of the Pan American Health Organization recommendations for prophylaxis and control, which led to a reduction in rabies cases from 1994 onwards. As a result of these measures, the rabies virus (RABV) genetic lineage associated with dogs has not been found in the state since 1998, and all the cases in domestic animals reported since then have been caused by bat-associated lineages of RABV. In the light of this, this study sought to investigate rabies cases in dogs and cats in the state of São Paulo between 2005 and 2014 and identify the associated transmission cycles by characterizing the RABV lineages responsible for these cases. Nine samples from dogs (n = 5) and from cats (n = 4) were collected between 2005 and 2014. The tenth animal, a rabid cat, was analysed by a different laboratory. The N gene nucleotide sequences obtained were analysed with the neighbor-joining algorithm and Kimura 2-parameter model using the MEGA 6 program. Phylogenetic analysis revealed that the genetic lineages identified in all the samples were those circulating in Brazilian bats. The findings of this study demonstrate that bats play an important role in the transmission of rabies to domestic animals in São Paulo state and that emphasis should be placed on the implementation of public policies to support surveillance of chiropterans for rabies.


Subject(s)
Cat Diseases/virology , Chiroptera/virology , Dog Diseases/virology , Rabies/veterinary , Animals , Brazil/epidemiology , Cat Diseases/epidemiology , Cat Diseases/etiology , Cats , Dog Diseases/epidemiology , Dog Diseases/etiology , Dogs , Phylogeny , Rabies/epidemiology , Rabies/transmission , Rabies/virology , Rabies virus/genetics , Rabies virus/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Time Factors
2.
Biologicals ; 41(4): 217-23, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23683880

ABSTRACT

Rabies is a widespread zoonotic disease responsible for approximately 55,000 human deaths/year. The direct fluorescent antibody test (DFAT) and the mouse inoculation test (MIT) used for rabies diagnosis, have high sensitivity and specificity, but are expensive and time-consuming. These disadvantages and the identification of new strains of the virus encourage the use of new techniques that are rapid, sensitive, specific and economical for the detection and research of the Rabies Virus (RABV). Real-time RT-PCR, phylogeographic analysis, proteomic assays and DNA recombinant technology have been used in research laboratories. Together, these techniques are effective on samples with low virus titers in the study of molecular epidemiology or in the identification of new disease markers, thus improving the performance of biological assays. In this context, modern advances in molecular technology are now beginning to complement more traditional approaches and promise to revolutionize the diagnosis of rabies. This brief review presents some of the recent molecular tools used for RABV analysis, with emphasis on rabies diagnosis and research.


Subject(s)
Biotechnology/methods , Fluorescent Antibody Technique , Molecular Epidemiology/methods , Rabies virus , Animals , Biomarkers/metabolism , Biotechnology/trends , Humans , Mice , Molecular Epidemiology/trends , Rabies/diagnosis , Rabies/epidemiology , Rabies/genetics , Rabies/immunology , Rabies/metabolism , Rabies virus/genetics , Rabies virus/immunology , Rabies virus/metabolism
3.
Virus Research ; 120(1-2): 113-120, sept. 2006. ilus, map, tab
Article in English | Sec. Est. Saúde SP, SESSP-IPPROD, Sec. Est. Saúde SP | ID: biblio-1068418

ABSTRACT

Rabies in wild canids in Northeastern Brazil is frequent and has been reported for some time, with episodes of rabies transmission from these animals to humans also reported. In this study, we analyzed the antigenic and genetic profiles of the rabies virus nucleoprotein gene, isolated from 20 samples taken from domestic animals and wild canids located in the Northeastern region of Brazil. All viruses isolated from domestic animals (dogs and cats) belonged to the antigenic variant 2 (AgV2). Among the wild animal samples, only four were AgV2, and nine showed a divergent antigenic profile. Phylogenetic analysis revealed two Brazilian clusters. Cluster 1 (Brazilian domestic carnivore-related strains) showed two subclusters, called 1A and 1B, and cluster 2 (Brazilian wild canid-related strains) also showed two subclusters, called 2A and 2B. The majority of the samples with divergent antigenic strains segregated into subcluster 2A. The intracluster identity of cluster 1 was 95.6% and that of cluster 2, 92.4%. When clusters 1 and 2 were compared, an identity of 88.6% was found. The genetic analysis of wild canid samples performed in this study indicates that there are two distinct rabies cycles among canids in Brazil, one represented by domestic canids and the other by wild canids. This study shows that the virus samples isolated in Northeastern Brazil are region and species-specific.


Subject(s)
Animals , Molecular Epidemiology , Phylogeny , Rabies virus , Brazil
5.
São Paulo; Instituto Pasteur;UNESP;Instituto Biólogico;SMS; 2000. 2 p.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-938035
6.
São Paulo; SMS; 2000.
Non-conventional in Portuguese | LILACS, Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-938267
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