ABSTRACT
AIM: The role of SMC apoptosis and proliferation was correlated to the amount of fibrillin and alfa-smooth muscle actin of primary varicose veins. METHODS: Twenty varicose vein specimens were atraumatically harvested from 20 women undergoing lower extremity primary varicose vein excision. The patients were divided into groups according to age (<50 years, >50 years) and the presence of leg edema (CEAP, class 2 or 3). The surface density of fibrillin-1 fibers (Sv([Fbn-1])), the volume density of smooth muscle cells: (Vv([SMC])), the number of proliferating and apoptotic cells per area. Quantitative data comparisons between class and age groups were performed. RESULTS: The median value of Vv([SMC]) was 16% greater and the Sv([Fbn-1]) was 35% greater in the intima vein sections from patients up to 50y compared to >50y. Apoptosis was found more frequent in veins sections from varicose women >50y. In the media layer, Sv([Fbn-1]) in veins from patients up to 50y was more important, and women with >50y had also more cells in apoptosis. Vv([SMC]) from women without edema (CEAP-Class 2) was 28% greater in the intima and apoptotic cells were more prominent in the intima of women with edema (CEAP-Class 2). In the media layer, Sv([Fbn-1]) was 12,5% greater in veins from women without edema and apoptosis was more detected in the veins from patients with edema. CONCLUSION: Age of the patient may affect the remodeling of varicose veins and SMC quantity in the media layer was found decreased in patients with edema.
Subject(s)
Actins/analysis , Apoptosis , Cell Proliferation , Microfilament Proteins/analysis , Muscle, Smooth, Vascular/chemistry , Muscle, Smooth, Vascular/pathology , Varicose Veins/metabolism , Varicose Veins/pathology , Adult , Age Factors , Brazil , Edema/etiology , Edema/metabolism , Edema/pathology , Female , Fibrillin-1 , Fibrillins , Humans , Hypertrophy , Immunohistochemistry , In Situ Nick-End Labeling , Middle Aged , Varicose Veins/complications , Varicose Veins/surgery , Veins/chemistry , Veins/pathology , Young AdultABSTRACT
A single dose of calcitonin (150 mIU/100 g body weight, sc) produced a significant decrease in liver antipyrine hydroxylase and aminopyrine demethylase activities. By contrast, pentobarbital sleeping time was not altered by calcitonin treatment. The present results indicate that acute calcitonin administration depresses the metabolism of substrates of the mixed function oxidase system of rat liver.
Subject(s)
Aminopyrine N-Demethylase/metabolism , Calcitonin/pharmacology , Liver/enzymology , Mixed Function Oxygenases/metabolism , Animals , Antipyrine/metabolism , Male , Pentobarbital/metabolism , Rats , Rats, Inbred Strains , Sleep/drug effectsABSTRACT
A single dose of calcitonin (150 mIU/100g body weight, sc) produced a significant decrease in liver antipyrine hydroxylase and aminopyrine demethylase activities. By contrast, pentobarbital sleeping time was not altered by calcitonin treatment. The present results indicate that acute calcitonin administration depresses the metabolism of substrates of the mixed function oxidase system of rat liver
