Lípidos poliinsaturados del tipo N-6 y expresión de P21 ras en carcinomas mamarios experimentales / Polyunsaturated n-6 lipids and expression of p21 RAS in experimental breast carcinomas

Se utilizó un modelo experimental de cáncer de mama inducido con dimetilbenz(a)antraceno en la rata, con el objetivo de determinar los efectos de una dieta rica en lípidos poliinsaturados del tipo n-6 sobre la expresión de la proteína oncogénica p21 Ras. La determinación de la expresión de p21 Ras se realizó mediante técnicas inmunohistoquímicas por el método de estreptavidina-biotina. Se analizaron 146 carcinomas: 19 del grupo control (C), 62 del grupo Inducción (I) y 65 del grupo Promoción (P). Los resultados mostraron que, aunque la media de la expresión de la proteína p21 Ras fue superior en los carcinomas del grupo P, no se obtuvieron diferencias significativas entre los grupos. Se concluye que los lípidos de la dieta del tipo n-6 no incrementaron significativamente los niveles de expresión de la proteína p21 Ras en las células tumorales de los grupos de ratas sometidas a dieta hiperlipídica(AU)

An experimental model of dimethylbenz[a]anthracene induced breast cancer in rats was used with the aim of determining the effects of a diet rich in type n-6 polyunsaturated lipids on the expression of the p21 Ras oncogenic protein. The determination of the p21 Ras was carried out through inmunohystochemical techniques, using the streptavidin-biotin method. One hundred and forty six carcinomas were analyzed: 19 from the control group (c), 62 from the induction group (l), and 65 from the Promotion group (P). The results showed that even though the mean for the p21 Ras protein expression was higher in the carcinomas from the group P, there were not significant differences among the groups. It was concluded that the lipids on the type n-6 diet did not increase significantly the p21 Ras protein expression levels in tumoral cells from the rat groups that underwent a hyperlipidic diet(AU)

Acute pseudobulbar palsy as the initial presentation of intravascular lymphomatosis.

We describe the case of a 76-year-old male patient who developed acute manifestations of pseudobulbar palsy without a history of stroke or vascular risk factors as the first manifestation of intravascular lymphomatosis. Neurological symptoms of acute pseudobulbar palsy appeared on the second postoperative day after transurethral prostatectomy for the treatment of prostatic hypertrophy. Intravascular lymphomatosis was diagnosed from microscopic findings and immunohistochemical staining of the surgical specimen. Chemotherapy was started, but the patient died due to sepsis secondary to bone marrow aplasia. Intravascular lymphomatosis should be considered a rare potential cause of acute pseudobulbar palsy.