ABSTRACT
OBJECTIVE To evaluate the efficacy and safety of meropenem as first choice for hospital acquired pneumonia(HAP) treatment in intensive care units(ICU) in Southwest Hospital. METHODS The usage of meropenem for the patients with HAP in ICU was studied by a prospective,open,and non-comparative trial.Prior to the clinical trial,the preliminary experiment on etiologic investigation was done through bronchoalveolar lavage and blood cultures.In the trial,all patients were treated intravenously with meropenem at a dose of 1g every 8 hours and(3 to 7 days) were established as a treatment duration period. RESULTS After the meropenem therapy,35 patients(66%) showed either cure or improvement.Mortality was 11% at the end of therapy.Clinical complications were observed in 11 patients(21%),with none of them definitely related to the study drug. CONCLUSIONS Meropenem is effective and well-tolerated as monotherapy for most HAP patients in our ICU.The low mortality rate in this study might have been attributed to the first choice use of this drug.
ABSTRACT
Objective:To investigate the expression of SLPI in dMSCs and the effect of SLPI on growth regulaton of dMSCs.Method :Isolated stem cells from the rat skin tissue.Two pieces of sterile polyvinyl alcohol sponges were inserted subcutaneously in the dorsum of the rat and collected the wound fluid 1day after injury.Isolated RNA of two groups:dMSCs before and after the stimulation by wound fluid;skin tissue before and after trauma.SLPI was detected to know how they changed in dMSCs and in the skin before and after the injure by using RT-PCR and Western blot method.[H3] Thymidine incorporation was used to evaluate the mitogenic influence of SLPI.Results:Most of the neonatal dMSCs were spindle-shaped cells and exhibited multilineage potential in vitro.It was shown in the result of RT-PCR and western blot that SLPI expressed in dMSCs and increased after the cells being stimulated by wound fluid.A concentration-dependent proliferation promotion were demonstrated in dMSCs incubated for 24h in the prsense of SLPI.Conclusions:SLPI can be detected in dMSCs and support the growth of dMSCs in serum-free medium in vitro.In view of the multiple functions of SLPI,we supposed that it acted as an important role when dMSCs participated in the reparing of cutaneous injury.
