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1.
East Mediterr Health J ; 17(1): 51-5, 2011 Jan.
Article in English | MEDLINE | ID: mdl-21735802

ABSTRACT

We compared light microscopy examination and a semi-nested multiplex PCR (SnM-PCR) assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small sub-unit ribosomal RNA (ssrRNA) gene sequences was applied. Microscopical examination showed that 48.5% (33.8% P. vivax and 14.7% P.falciparum) and 50% (35.3% P. vivax and 14.7% P. falciparum) of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy.


Subject(s)
Malaria, Falciparum/diagnosis , Malaria, Vivax/diagnosis , Microscopy/instrumentation , Plasmodium falciparum/isolation & purification , Plasmodium vivax/isolation & purification , Polymerase Chain Reaction/instrumentation , Adolescent , Adult , Child , Child, Preschool , DNA, Protozoan/isolation & purification , Female , Humans , Male , Middle Aged , Plasmodium falciparum/genetics , Plasmodium vivax/genetics , Young Adult
2.
(East. Mediterr. health j).
in English | WHO IRIS | ID: who-118078

ABSTRACT

We compared light microscopy examination and a semi-nested multiplex PCR [SnM-PCR] assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small subunit ribosomal RNA [ssrRNA] gene sequences was applied. Microscopical examination showed that 48.5% [33.8% P. vivax and 14.7% P.falciparum] and 50% [35.3% P. vivax and 14.7% P.falciparum] of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy


Subject(s)
Malaria, Vivax , Microscopy , Polymerase Chain Reaction , Malaria, Falciparum
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