Effects of FABP2 Ala54Thr gene polymorphism on obesity and metabolic syndrome in middle-aged Korean women with abdominal obesity.

OBJECTIVES: Asians (including Chinese, Japanese and Koreans), who generally have a relatively smaller body size and a lower mean body mass index (BMI), have a relatively higher risk of developing android-type obesity than westerners. Substitution of alanine for threonine (Ala54Thr) on the FABP2 gene (rs 1799883) is related to insulin resistance and obesity. However, few studies have examined this substitution in Koreans, and the number of Korean subjects in those studies is limited. For this reason, we investigated the differences between the FABP2 Ala54Thr polymorphism and obesity, hemodynamic variables, blood lipid profile results, and insulin resistance among middle-aged Korean women with abdominal obesity. METHODS: We studied 243 middle-aged community-dwelling Korean women with abdominal obesity from Gyeonggi Province, Republic of Korea, who had no history of taking chronic medications. We examined each subject (n = 243) for the presence of FABP2 Ala54Thr polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Subjects were also examined for obesity hemodynamic variables (n = 243), lipid profiles (n = 142), and insulin resistance (n = 142). RESULTS: Of the 243 subjects, 117 had AA ("normal") homozygotic genotype, 100 had AT heterozygotic genotype, and 26 had TT homozygotic genotype for the FABP2 Ala54Thr polymorphism. The AT heterozygotic individuals had a significantly higher mean waist-to-hip ratio, abdominal fat area, and visceral fat area than individuals with other genotypes. TT homozygotic individuals had higher mean triglyceride and fasting glucose levels than individuals with other genotypes. CONCLUSIONS: The results of this study show that the FABP2 Ala54Thr polymorphism was associated with central obesity and obesity-related metabolic syndrome among middle-aged Korean women.

Treadmill exercise restores high fat diet-induced disturbance of hippocampal neurogenesis through ß2-adrenergic receptor-dependent induction of thioredoxin-1 and brain-derived neurotrophic factor.

A high-fat diet (HFD) is known to induce metabolic disturbances that may lead to cognitive impairment. In the present study, we investigated whether a regular treadmill exercise program would improve HFD-induced hippocampal-dependent memory deficits in C57BL/6 mice. Weight gain and hepatic triglyceride levels were profoundly elevated following administration of a 60% HFD for 23 weeks, and this change was attenuated by 23-weeks of treadmill running. The exercise regimen attenuated impairments in memory function of HFD-fed mice in a water maze test and recovered HFD-induced anti-neurogenic effects as shown by immunohistochemistry data with Ki-67 and doublecortin (DCX) antibodies. Moreover, the treadmill exercise resulted in anti-inflammatory, antioxidant, and neuroprotective effects in the HFD-fed brain. The exercise inhibited HFD-induced microglial activation, expression of proinflammatory cytokines (tumor necrosis factor-α and interleukin-1ß), and NF-κB activity in the dentate gyrus (DG) of the hippocampus. In addition, the exercise reduced malondialdehyde levels elevated by HFD and recovered antioxidant superoxide dismutase and glutathione levels in hippocampal DG of HFD-mice. The exercise also reduced the number of apoptotic cells induced by HFD, as shown by TUNEL staining in the DG region. Finally, we demonstrated that the thioredoxin-1 (TRX-1) and brain-derived neurotrophic factor (BDNF) levels were recovered by exercise, which was demonstrated to act via ß2-adrenergic receptor enriched in synaptosomes of the DG. Therefore, our data collectively suggests that regular exercise may be a promising approach to preventing HFD-induced memory impairments via anti-inflammatory, antioxidant and neuroprotective mechanisms in the hippocampal DG region.

Comparison of the effects of acute exercise after overnight fasting and breakfast on energy substrate and hormone levels in obese men.

[Purpose] We compared the effects of acute aerobic exercise following overnight fasting and breakfast on energy substrate and hormone levels in obese male college students. [Subjects and Methods] This crossover study recruited 10 obese male college students with a body mass index >25 kg/m(2) or >20% body fat. One week post-recruitment, the subjects exercised in the morning after an overnight fast. At 2 weeks, they exercised post-breakfast. Energy substrate (glucose, free fatty acid) and metabolic hormone (insulin, growth hormone, and cortisol) levels were measured immediately before and after exercise and at 60 min post-exercise. [Results] We observed interaction effects between the measurement time and exercise treatment for glucose; significant differences between measurement times and between exercise treatments for free fatty acids; interaction effects between the measurement time and exercise treatment for insulin and significant differences in the measurement time; significance differences between measurement times and between exercise treatments for growth hormone; and significant differences between measurement times and between exercise treatments for cortisol. [Conclusion] Morning exercise following an overnight fast can be more effective in reducing body fat than post-prandial exercise. However, increased cortisol levels following exercise after overnight fasting may negatively affect long-term weight loss in obese men.

Chronic exercise prevents repeated restraint stress-provoked enhancement of immobility in forced swimming test in ovariectomized mice.

We assessed whether chronic treadmill exercise attenuated the depressive phenotype induced by restraint stress in ovariectomized mice (OVX). Immobility of OVX in the forced swimming test was comparable to that of sham mice (CON) regardless of the postoperative time. Immobility was also no difference between restrained mice (exposure to periodic restraint for 21 days; RST) and control mice (CON) on post-exposure 2nd and 9th day, but not 15th day. In contrast, the immobility of ovariectomized mice with repeated stress (OVX + RST) was profoundly enhanced compared to ovariectomized mice-alone (OVX), and this effect was reversed by chronic exercise (19 m/min, 60 min/day, 5 days/week for 8 weeks; OVX + RST + Ex) or fluoxetine administration (20 mg/kg, OVX + RST + Flu). In parallel with behavioral data, the immunoreactivity of Ki-67 and doublecortin (DCX) in OVX was significantly decreased by repeated stress. However, the reduced numbers of Ki-67- and DCX-positive cells in OVX + RST were restored in response to chronic exercise (OVX + RST + Ex) and fluoxetine (OVX + RST + Flu). In addition, the expression pattern of cAMP response element-binding protein (CREB) and calcium-calmodulin-dependent kinase IV (CaMKIV) was similar to that of the hippocampal proliferation and neurogenesis markers (Ki-67 and DCX, respectively). These results suggest that menopausal depression may be induced by an interaction between repeated stress and low hormone levels, rather than a deficit in ovarian secretion alone, which can be improved by chronic exercise.

Association between serum vitamin D status and metabolic syndrome in Korean young men.

PURPOSE: This study examined the relations of serum vitamin D levels to body fatness, cardiorespiratory fitness (CRF), and metabolic risk factors in young adults in Korea. METHODS: Between 2007 and 2009, 799 young men completed a health examination. Body fatness, CRF based on a maximal treadmill exercise test, and measurements of metabolic risk factors were measured in study participants. Participants were classified by serum vitamin D levels as deficient (<12.5 ng·mL), insufficient (≥12.5 to <20 ng·mL), and sufficient (>20 ng·mL) and by CRF as unfit (lowest 20%) and fit (remaining 80%) based on age-standardized distribution of V˙O2max values in this study population. Body fatness, CRF, and metabolic risk factors were evaluated according to serum vitamin D classification. A clustered metabolic risk score was computed by summing standardized scores for waist circumference, resting blood pressures, triacylglycerols, the inverse of high-density lipoprotein cholesterol, glucose, and insulin. RESULTS: Linear decreases in body fatness and metabolic risk factors were observed, as was a linear increase for CRF across incremental vitamin D categories. A linear decrease was found in the clustered metabolic risk score across incremental vitamin D categories. Compared to the fit group (reference), the unfit group had significantly higher risks for serum vitamin D inadequacy before and after adjusting for age, smoking, and body fatness parameters. CONCLUSIONS: The findings of the study suggest that increasing vitamin D intake, eating a healthy diet, and getting enough outdoor physical activity should be promoted as nonpharmacologic means to improve CRF and prevent a clustering of metabolic risk factors in young adults.

Effects Ala54Thr polymorphism of FABP2 on obesity index and biochemical variable in response to a aerobic exercise training.

The purpose of the current study was to investigate whether or not the FABP2 gene polymorphism modulated obesity indices, hemodynamic factor, blood lipid factor, and insulin resistance markers through 12-week aerobic exercise training in abdominal obesity group of Korean mid-life women. A total of 243 abdominally obese subjects of Korean mid-life women voluntarily participated in aerobic exercise training program for 12 weeks. Polymerase Chain Reaction with Restriction Fragment Length Polymorphism (PCR-RFLP) assay was used to assess the FABP2 genotype of the participants (117 of AA homozygotes, 100 of AT heterozygotes, 26 of TT homozygotes). Prior to the participation of the exercise training program, baseline obesity indices, hemodynamic factor, blood lipid factor, and insulin resistance markers were measured. All the measurements were replicated following the 12-week aerobic exercise training program, and then the following results were found. After 12-week aerobic exercise training program, wild type (Ala54Ala) and mutant type (Ala54Thr+Thr54Thr) significantly decreased weight (P > .001), BMI (P > .001), %bf (P > .001), waist circumference (P > .001), WHR (P > .001), muscle mass (wild type p < .022; mutant type P > .001), RHR (P > .001), viseceral adipose area (wild type p < .005; mutant type P > .001), subcutaneous area (P > .001), insulin (wild type p < .005; mutant type P > .001) and significantly increased VO2max (P > .001). And wild type significantly decresed NEFA (P > .05), glucose (P > .05), OGTT 120min glucose (P > .05) and significantly increased HDLC (p > .005). Mutant type significantly decreased SBP (P > .001), DBP (P > .01), TC (P > .01), LPL (P > .05), LDL (P > .001), HOMA index (P > .01). The result of the present study represents that regular aerobic exercise training may beneficially prevent obesity index, blood pressure, blood lipids and insulin resistance markers independent of FABP Ala54Thr wild type and mutant type.

Combined effects of body mass index and cardio/respiratory fitness on serum vaspin concentrations in Korean young men.

Little information is available regarding how body fatness and cardio/respiratory fitness (CRF) are associated with serum vaspin. The purpose of the study was to investigate the combined effect of body mass index (BMI) and CRF on serum vaspin in Korean young men. In a cross-sectional study, we examined 490 Korean young men (mean age 23.8 +/- 2.5 years) who were voluntarily recruited. Body fatness and fasting levels of serum insulin, adiponectin, and vaspin were measured. CRF was quantified as the minute volume of oxygen consumption (VO(2)) measured during a graded treadmill test. We assigned individuals to either low or middle or high third CRF tertiles based on age-adjusted VO(2max). We also assigned individuals to either a lean weight (LN) or obese (OB) group based on body fatness levels, in which a BMI value >/=25 kg/m(2) was used as an indicator of Pacific-Asian obesity. Group analyses showed significant interaction effects between fatness and CRF on fasting insulin and serum vaspin such that the OB group with low CRF levels had significantly higher insulin and vaspin concentrations than the OB counterparts with moderate to high CRF levels, and no such CRF-based sub-group differences in insulin and vaspin were found in the LN groups. Regression analyses show that BMI, waist circumference, VO(2), and fasting insulin explain approximately 18% of the individual variations in serum vaspin concentration in this study population. This is the first study to show that high body fatness along with low CRF might contribute to increased vaspin concentrations in Korean young men.