ABSTRACT
<p><b>OBJECTIVE</b>To explore a new method for the therapy of avascular necrosis of the femoral head.</p><p><b>METHOD</b>The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Repair of the femoral head was observed by histological and histomorphometric analysis.</p><p><b>RESULT</b>Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histomorphometric analysis (P< 0.01).</p><p><b>CONCLUSION</b>Transfection of bFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly.</p>
Subject(s)
Animals , Rabbits , Collagen , Therapeutic Uses , Femur Head , Metabolism , Femur Head Necrosis , Therapeutics , Fibroblast Growth Factor 2 , Genetics , Gene Expression , Genetic Therapy , Neovascularization, Physiologic , Osteogenesis , Plasmids , Recombinant Proteins , Genetics , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.</p><p><b>RESULTS</b>The proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.</p><p><b>CONCLUSION</b>Blockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.</p>
Subject(s)
Humans , Antineoplastic Agents , Pharmacology , Antisense Elements (Genetics) , Genetics , Autocrine Communication , Bone Neoplasms , Metabolism , Pathology , Cell Division , Cell Line, Tumor , Doxorubicin , Pharmacology , Osteosarcoma , Metabolism , Pathology , RNA, Messenger , Genetics , Transfection , Transforming Growth Factor beta , Genetics , Transforming Growth Factor beta1ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>
