ABSTRACT
@#BACKGROUND. Caseinophosphopeptides (CPPs) were released during in vivo digestion process under the influence of the gut proteolytic enzymes. CPPs contain some phosphorylated serine residues which are able to connect calcium ions. This can predict the bone development and osteoporosis prevention effects of CPPs. MATERIAL AND METHODS. In our study we have used acid caseins, from cow and camel milk, which were then hydrolysed using pancreatin preparation in vitro. RESULTS. The AN/TN rate of resulting hydrolysates were 3.8 and 3.2, subsequently. Then CPPs were precipitated by adding calcium chloride and ethanol and the yield of both types of CPPs was the same (16%). The ratio Ca:P were 2.8:1 and 2.7:1 for cow and camel peptide preparations, subsequently. СONCLUSION. These results show the real potential for obtaining calcium substance on the base of CPPs.
ABSTRACT
Spent brewer’s yeast Saccharomyces cerevisiae was hydrolysed using bovine pancreatictissue as an enzyme source. The resulting hydrolysate contains 4.7% amino nitrogenand the ratio Namin/Ntot was determined as 0.6. Moreover, other physico-chemicalparameters of the hydrolysate were also comparable with the same products on foreignmarkets. Toxicity of hydrolysate is low (LD50 2,5 g/kg), microbiological and heavy metalcontamination were in required range. The IC50 value of obtained yeast hydrolysate’santioxidative activity according to the DPPH assay was 1.6±0.5 mg/ml, while IC¬50 ofthe yeast hydrolysate rich in Cyclo-His-Pro evaluated as 1.9 mg/mL.
