ABSTRACT
Context: Since there is still a great need to search for plant species with antinociceptive and anti-inflammatory activities, Diploptropis purpurea (Rich.) Amshoff (Fabaceae) is studied for the first time. Objective: This evaluates the analgesic and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea (MEDP). Material and methods: The anti-inflammatory and analgesic effects of MEDP of D. purpurea were evaluated in vivo. The antinociceptive activity was assessed in CD1 male mice were treated by oral gavage with 500 mg/kg of MEDP 30 min before submitting to acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Paws oedema induced by carrageenan, histamine or serotonin were performed in male Sprague-Dawley rats to determinate the anti-inflammatory activity. Results: Oral administration of MEDP produced significant antinociceptive effects on the inflammatory phase in the formalin test [12.0 s versus 72.5 s in carboxymethyl cellulose (CMC) control group]. MEDP produced an analgesic effect in the hot-plate model, although the effect was modest compared to tramadol (40 and 60%, respectively). The oral administration of MEDP in a dose of 500 mg/kg showed maximum inhibition (75.1%) after 0.5 h in carrageenan-induced oedema, but it did not modify histamine or serotonin-induced oedemas. Discussion and conclusion: In the peripheral nociception model, acetic acid-induced abdominal writhing, the MEDP did not show a protective effect, but its analgesic effects were evident in the inflammatory phase of the formalin test and in the hot-plate model. These results show that the anti-inflammatory effect was accompanied by a reduction in the perception of painful stimuli.
Subject(s)
Analgesics/pharmacology , Anti-Inflammatory Agents/pharmacology , Edema/drug therapy , Fabaceae/chemistry , Pain/drug therapy , Plant Extracts/pharmacology , Animals , Edema/chemically induced , Inflammation/drug therapy , Male , Methanol/chemistry , Mice , Pain/chemically induced , Pain Measurement , Plant Extracts/isolation & purification , Plant Leaves/chemistry , Rats , Rats, Sprague-DawleyABSTRACT
Carvone has corroborated its versatility as starting material for building blocks synthesis in organic chemistry, being achieved a new chiral lactone. It has been done a study on the antioxidant activity against superoxide of carvone and a chloro derivative that show the potent activity of the natural product (R)-carvone, 1.
Subject(s)
Antioxidants/chemistry , Monoterpenes/chemistry , Superoxides/chemistry , Biological Products , Cyclohexane Monoterpenes , Molecular StructureABSTRACT
Vasorelaxant activity Vasorelaxant effects of eight diterpenoids isolated from three Venezuelan plants [(+)-manool [(+)-labda-8(17),14-dien-13-ol], (+)-manoyl oxide, (+)-2-oxomanoyl oxide, sandaracopimara-8(14), 15-dien-3β, 19-diol, jhanidiol acetate (18-acetoxy-1βhydroxymanoyl oxide), jhanidiol (1β,18-dihydroxymanoyl oxide), ent-kaur-16-en-19ol and grandiflorenic acid (ent-kaur-9(11),16-dien-19-oic acid)] aortic rings were assessed in intact endothelium and endothelium-denuded isolated rat. Thw cumulative addition (10-6 to 10-4 M) of each product were carried out after contraction with phenylephrine (10-6 M). Jhanidiol acetate and ent-kaur-9,16-en-19-oic acid at 10-4 M dose concentration, exhibit the maximal vasorelaxant effect in endothelium-intact rings (51.61 ± 7.62% and 79.27 ± 7.41%, respectively). In endothelium-denuded aortic rings, the maximum vascular response exerted by both compounds was not abolished (64.14 ± 5.64% and 84.84 ± 3.62%, respectively). In denuded aortic rings, the half-maximal inhibitory concentration (IC50) Jhanidiol was obtained by the ethyl less than those obtained in rings endothelium (1.09 × 10-4 vs 7.29 × 10-5 M, respectively), although this difference was not significant. These results suggested that the mechanism behind the vasorelaxant effect of the two diterpene is mediated by endothelium-independent pathways.
