ABSTRACT
Una de las complicaciones de la radioterapia y la quimioterapia es la mucositis oral. Dado que el láser de baja energía es una de las intervenciones más frecuentemente recomendadas por los autores y las sociedades internacionales, el objetivo de esta revisión bibliográfica es el de revisar la evidencia científica existente sobre el empleo del láser de modo preventivo y terapéutico en la mucositis oral asociada al tratamiento del cáncer. Se realizó una búsqueda bibliográfica en PubMed y en la biblioteca de The Cochrane Collaboration, limitando la búsqueda a los últimos 20 años. Finalmente se incluyeron 29 artículos, que contenían 30 estudios. La fototerapia con láser de baja energía parece una intervención prometedora tanto en la prevención como en el tratamiento de la mucositis oral asociada al tratamiento del cáncer. Prácticamente todos los estudios revisados encontraron buenos resultados, sin efectos adversos y con reducciones tanto de la incidencia como de la gravedad de la mucositis en todos los tipos de tratamientos del cáncer (AU)
One of the complications of radiotherapy and chemotherapy is oral mucositis. Since the low energy laser is one of the mostfrequently recommended interventions by authors and international societies, the aim of this study is to review the scientific evidence on the use of lasers as a preventive and therapeutic in oral mucositis associated with treatment of cancer. We performed a literature search in PubMed and The cochrane Collaboration Library, limiting the search to the last 20 years. We finally included 29 articles that contained 30 studies. Low energy laser phototherapy seems a promising intervention in both the prevention and treatment of oral mucositis associated with cancer treatment. Virtually all studies reviewed showed good results with no adverse effects and reductions in both incidence and severity of mucositis in all types of cancer treatments (AU)
Subject(s)
Humans , Laser Therapy/methods , Stomatitis/therapy , Head and Neck Neoplasms/complications , Radiotherapy/adverse effects , Antineoplastic Agents/adverse effects , Evidence-Based PracticeABSTRACT
One of the complications of radiotherapy and chemotherapy is oral mucositis. Since the low energy laser is one of the most frequently recommended interventions by authors and international societies, the aim of this study is to review the scientific evidence on the use of lasers as a preventive and therapeutic in oral mucositis associated with treatment of cancer. We performed a literature search in PubMed and The Cochrane Collaboration Library, limiting the search to the last 20 years. We finally included 29 articles that contained 30 studies. Low energy laser phototherapy seems a promising intervention in both the prevention and treatment of oral mucositis associated with cancer treatment. Virtually all studies reviewed showed good results with no adverse effects and reductions in both incidence and severity of mucositis in all types of cancer treatments.
Subject(s)
Antineoplastic Agents/adverse effects , Cranial Irradiation/adverse effects , Head and Neck Neoplasms/complications , Low-Level Light Therapy , Stomatitis/prevention & control , Stomatitis/therapy , Bone Marrow Transplantation , Case-Control Studies , Chemoradiotherapy/adverse effects , Double-Blind Method , Head and Neck Neoplasms/drug therapy , Head and Neck Neoplasms/radiotherapy , Humans , Low-Level Light Therapy/methods , Low-Level Light Therapy/statistics & numerical data , Prospective Studies , Randomized Controlled Trials as Topic , Stomatitis/etiology , Treatment OutcomeABSTRACT
Somatostatin/growth hormone-inhibiting hormone is the peptide that inhibits secretion of somatotropin/growth hormone. Solid-phase synthesis methods are being currently used to produce somatostatin. Recombinant peptide synthesis is widely described for the production of small proteins and peptides; however, the production at industrial scale of peptides for biopharmaceutical applications is limited for economic reasons. Here, we propose the use of a new pGB-SMT plasmid to produce Somatostatin, as a C-terminal fusion protein with a Kluyveromyces lactis ß-galactosidase fragment. To facilitate removal of that fragment by CNBr cleavage, a methionine residue was introduced at the N-terminal of the hormone peptide. The use of this construction enables an IPTG-free expression system. The suitability of this procedure has been assessed in a 15 l scale-up experiment yielding almost 300 mg, with purity >99 % and it is being implemented for commercial scale. The plasmid pGB-SMT here described is an alternative option for a cheap and high expression of other short peptide hormones.
Subject(s)
Escherichia coli/genetics , Genetic Vectors , Somatostatin/biosynthesis , Somatostatin/isolation & purification , Chromatography, Reverse-Phase , Cloning, Molecular , Escherichia coli/metabolism , Gene Expression , Humans , Methionine/metabolism , Plasmids , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Somatostatin/genetics , beta-Galactosidase/genetics , beta-Galactosidase/metabolismABSTRACT
Actualmente, existen suficientes investigaciones que sostienen que incorporar a la dieta ciertos alimentos ricos en antioxidantes, tiene un efecto beneficioso en la salud en general. Una dieta equilibrada disminuye el riesgo de patologías tales como las enfermedades cardiovasculares, cáncer y diabetes, entre otras. También el papel de los antioxidantes en la salud oral, es objeto de estudio en este momento. Se buscan beneficios y aplicaciones en la prevención y tratamiento de patologías orales, que de ser demostrados podrían suponer en el futuro un cambio en el manejo de estas patologías. Se ha realizado una revisión de la literatura para determinar si existe suficiente evidencia científica que avale la presencia de propiedades preventivas y terapéuticas de los antioxidantes en la salud oral. Para ello, se han consultado las bases de datos PubMed/Medline y Cochrane con las palabras clave: antioxidants, oral health, oral cancer, nutrition, quimioprevention, leukoplakia, lycopene (AU)
Nowadays, it is proved that incorporating food which is rich in antioxidants has beneficial effects to health. A balanced diet decreases the risk of suffering pathologies as heart disease, cancer and diabetes among others. In addition, the role of antioxidants in oral health is currently understudy. The aim of this paper is to find benefits and applications in the prevention and treatment of oral pathologies. If this was proved it could mean a future change in these pathologies management. A review of the scientific evidence todeterminate whether antioxidants have preventive and therapeutic properties in oral health hasbeen done, using the data bases PubMed/Medline, Cochrane with the following key words:antioxidants, oral health, oral cancer, nutrition, quimioprevention, leukoplakia, lycopene (AU)
Subject(s)
Antioxidants/physiology , Antioxidants/therapeutic use , Oral Health , Diet/methods , Diet Therapy , Leukoplakia, Oral/diet therapy , Chemoprevention , 52503/physiology , Antioxidants/administration & dosage , Dental Clinics/methods , Carotenoids/therapeutic useABSTRACT
A total of 6047 open reading frames in the Candida albicans genome were screened for Zn(II)(2)C(6)-type zinc cluster proteins (or binuclear cluster proteins) involved in DNA recognition. These fungal proteins are transcription regulators of genes involved in a wide range of cellular processes, including metabolism of different compounds such as sugars or amino acids, as well as multi-drug resistance, control of meiosis, cell wall architecture, etc. The selection criteria used in the sequence analysis were the presence of the CysX(2)CysX(6)CysX(5-16)CysX(2)CysX(6-8)Cys motif and a putative nuclear localization signal. Using this approach, 70 putative Zn(II)(2)C(6) transcription factors have been found in the genome of C. albicans.
ABSTRACT
BACKGROUND: The emergence and re-emergence of infectious diseases are a major concern in Public Health. The unique socio-demographic aspects of the Autonomous Community of Madrid make it necessary for a greater deal of attention to be paid to pet-transmitted zoonoses. This study is aimed as heightening the knowledge of the emerging and re-emerging pet-transmitted zoonoses and the design of a method for prioritizing the same. METHODS: Based on the diseases obtained from official sources, laboratories and a review of published studies, a quantification and weighting method designed by the working group and adapted to the specificity of this study was applied. RESULTS: Based on the analysis of 137 diseases, 24 met the admission requirements. The weighting method is provided in table form, including eleven scoring criteria, the criteria categories and the coefficients employed. Salmonellosis was the top-ranked disease, followed by Q Fever, Tularemia and Hantavirus Infection. CONCLUSIONS: A method for specifically evaluating emerging and re-emerging diseases was designed, affording the possibility of setting priorities in the Public Health planning field. This study provides a listing of 24 zoonoses ranked in order of importance, based on which new strategy-related lines must be set out for the research and/or control thereof.
