Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins.

Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 µM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination.

Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses.

Listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. In this context, this study aimed to evaluate the anti-listerial action of bacteriocins produced by Enterococcus lactis LBM BT2 in an alternative medium containing sugarcane molasses (SCM). Molecular analyses were carried out to characterize the strain, including the presence of bacteriocin-related genes. In the kinetic study on SCM medium E. lactis, LBM BT2 showed biomass and bacteriocin productions similar to those observed on a sucrose-based medium (control), highlighting the potential of the sugarcane molasses as a low-cost substrate. Stability tests revealed that the molecule remained active in wide ranges of pH (4-10) and temperature (60-100 °C). Furthermore, the proteolytic treatment reduced the biomolecule's antimicrobial activity, highlighting its proteinaceous nature. After primary purification by salting out and tangential flow filtration, the bacteriocin-like inhibitory substance (BLIS) showed bacteriostatic activity on suspended L. monocytogenes cells and against biofilm formation at a concentration of 0.625 mg/mL. These results demonstrate the potential of the produced BLIS as a biopreservative in the food industry.

Tinnitus Footprints in the Cochlea.

The purpose of this paper is to show how temporal bone histopathology has been instrumental in adding knowledge about the origin of tinnitus in the cochlea and how it will still be useful for that purpose in the future. The papers published on this subject will be reviewed, and their contributions will be highlighted. The knowledge that is now part of the subject will be pointed out, and future research on this area will be pointed out.

Dual antibacterial mechanism of [K4K15]CZS-1 against Salmonella Typhimurium: a membrane active and intracellular-targeting antimicrobial peptide.

Salmonella genus is a leading cause of food-borne infections with strong public health impact and economic ramifications. The development of antimicrobial resistance added complexity to this scenario and turned the antibiotic drug discovery into a highly important challenge. The screening of peptides has served as a successful discovery platform to design new antibiotic candidates. Motivated by this, the antimicrobial and cytotoxic properties of three cruzioseptins against Salmonella Typhimurium and RAW 264.7 murine macrophage cells, respectively, were investigated. [K4K15]CZS-1 was the most potent antimicrobial peptide identified in the screening step with a minimum inhibitory concentration (MIC) of 16 µg/mL (7.26 µM) and moderate cytotoxicity. From a structural point of view, in vitro and in silico techniques evidenced that [K4K15]CZS-1 is a α-helical cationic antimicrobial peptide. In order to capture mechanistic details and fully decipher their antibacterial action, we adopted a multidimensional approach, including spectroscopy, electron microscopy and omics analysis. In general lines, [K4K15]CZS-1 caused membrane damage, intracellular alterations in Salmonella and modulated metabolic pathways, such as the tricarboxylic acid (TCA) cycle, fatty acid biosynthesis, and lipid metabolism. Overall, these findings provide deeper insights into the antibacterial properties and multidimensional mode of action of [K4K15]CZS-1 against Salmonella Typhimurium. In summary, this study represents a first step toward the screening of membrane-acting and intracellular-targeting peptides as potential bio-preservatives to prevent foodborne outbreaks caused by Salmonella.

Nivolumab for newly and recurrent glioblastoma multiforme treatment: A systematic review and meta-analysis.

OBJECTIVE: We conducted a systematic review and meta-analysis aiming to assess the efficacy and safety of Nivolumab treatment in patients with newly diagnosed and recurrent glioblastoma multiforme (GBM). DATA SOURCES: Our study followed the guidelines outlined in the preferred reporting items for systematic reviews and meta-analyses (PRISMA) recommendations. The protocol for this review can be found in the International Prospective Register of Systematic Reviews Database (CRD42022340071). We performed searches on the Medline, PubMed, Embase, Scopus, and Web of Science databases. DATA SUMMARY: A total of 545 studies were identified through our comprehensive search across the five databases (PubMed: 78, Embase: 82, Medline: 173, Scopus: 138, Web of Science: 74). After conducting a thorough analysis, our meta-analysis indicated that treatment with Nivolumab led to improved overall survival (OS) outcomes in newly diagnosed glioblastoma patients, as evidenced by a prolonged median OS based on trial data. However, there was no significant beneficial effect observed in terms of median progression-free survival (PFS), as well as OS at 6, 12, and 24 months. Furthermore, our results demonstrated no efficacy of Nivolumab in the treatment of recurrent GBM patients. CONCLUSIONS: In conclusion, Nivolumab demonstrated promising results that warrant further investigation for its use in newly diagnosed glioblastoma patients. However, its effectiveness was not observed in the context of recurrent GBM.

Sunflower cake with or without enzymatic complex for broiler chickens feeding.

OBJECTIVE: This study was to evaluate the sunflower cake and enzymatic complex fed to broilers from 22 to 42 d of age. METHODS: In a completely randomized design, a total of 850 birds were allotted in a 2×5 factorial scheme (with and without enzymatic complex) and five inclusion levels (0%, 5%, 10%, 15%, 20%) of sunflower cake. There were 5 replications and 17 birds in each experimental unit. Data from performance, carcass yield and intestinal morphology were evaluated. RESULTS: Feed intake, weight gain, final weight and feed:gain ratio linearly worsened as sunflower cake increased. For weight gain, final weight and feed:gain ratio, the birds whose diets contained levels of 15% and 20% of sunflower cake showed worse values (p<0.05) than the birds fed the control diet. When fed the enzymatic complex, birds improved (p<0.05) crypt depth and villus:crypt ratio in the jejunum. As inclusion levels of sunflower cake increased, villus depth and villus:crypt ratio in duodenum, jejunum and ileum linearly reduced and the crypt depth linearly increased. Carcass yield linearly reduced as sunflower cake increased. CONCLUSION: Based on performance, sunflower cake can be used up to 10% in broilers feeding from 21 to 42 days of age.

Pesquisando com professores: a centralidade do diário de campo e da restituição em uma pesquisa-intervenção / Researching with teachers: the field diary and restitution's importance in the intervention research

A pesquisa "Pesquisando com Professores: a relação mídia e cotidiano escolar" analisou com 25 professores de uma escola pública estadual de Fortaleza a relação entre mídia e cotidiano escolar. Como dispositivo de pesquisa foi criado o curso de extensão "Diálogos Escolares: Mídia e Cotidiano Escolar". Com base em Michel Foucault (1987) acerca do poder disciplinar presente na educação escolar e em Gilles Deleuze (1990) sobre as novas formas de controle em que participam a mídia, a pesquisa discute, em última instância, a função social da escola na contemporaneidade, problematizando a mídia como vetor de subjetivação no cotidiano escolar e no fazer pedagógico. No presente artigo colocamos em análise a metodologia da pesquisa, dando ênfase à centralidade do "diário de campo" e da "restituição". Trabalharemos também o conceito de pesquisa-intervenção e sua implicação prática como estratégia da pesquisa com a escola. Discutiremos o uso do diário de campo como uma ferramenta facilitadora de vínculo e de construção de dados necessário para ressignificação da mesma. Destacaremos a restituição como o momento necessário para criarmos um campo de análise coletiva. Por fim, apresentaremos a importância dessas ferramentas para todo o processo de construção da nossa pesquisa.

The search "Searching with teachers: media relations and school routine" has analyzed with 25 teachers from a public school in Fortaleza, the relationship between media and school routine. As a research device was created the extension course "School Dialogues: Media and School Daily Life". Based on Michel Foucault (1987) on the disciplinary power present in school education and Gilles Deleuze (1990) on new ways to control the media involved, the research argues, ultimately, the school's social function in contemporary, questioning the media as subjectivity vector in everyday school life and pedagogical work. In this paper we put in question the research methodology, emphasizing the centrality of the diary and restitution. We will also work the concept of intervention research and its practical implication as research strategy with the school. We will discuss the use of the diary as a facilitating tool bond and construction data needed to reframe the same. We highlight restitution as the necessary time to create a collective analysis field. Finally, we will present the importance of these tools for the entire process of building our research.

Pesquisando com professores: a centralidade do diário de campo e da restituição em uma pesquisa-intervenção / Researching with teachers: the field diary and restitution’s importance in the intervention research

A pesquisa “Pesquisando com Professores: a relação mídia e cotidiano escolar” analisou com 25 professores de uma escola pública estadual de Fortaleza a relação entre mídia e cotidiano escolar. Como dispositivo de pesquisa foi criado o curso de extensão “Diálogos Escolares: Mídia e Cotidiano Escolar”. Com base em Michel Foucault (1987) acerca do poder disciplinar presente na educação escolare em Gilles Deleuze (1990) sobre as novas formas de controle em que participam a mídia, a pesquisa discute, em última instância, a função social da escola na contemporaneidade, problematizando a mídia como vetor de subjetivação no cotidiano escolar e no fazer pedagógico. No presente artigo colocamos em análise a metodologia da pesquisa, dando ênfase à centralidade do “diário de campo” e da“restituição”. Trabalharemos também o conceito de pesquisa-intervenção e sua implicação prática como estratégia da pesquisa com a escola. Discutiremos o uso do diário de campo como uma ferramenta facilitadora de vínculo e de construção de dados necessário para ressignificação da mesma. Destacaremos a restituição como o momento necessário para criarmos um campo de análise coletiva. Por fim, apresentaremos a importância dessas ferramentas para todo o processo de construção da nossa pesquisa.(AU)

Pesquisando com professores: a centralidade do diário de campo e da restituição em uma pesquisa-intervenção / Researching with teachers: the field diary and restitution’s importance in the intervention research

A pesquisa “Pesquisando com Professores: a relação mídia e cotidiano escolar” analisou com 25 professores de uma escola pública estadual de Fortaleza a relação entre mídia e cotidiano escolar. Como dispositivo de pesquisa foi criado o curso de extensão “Diálogos Escolares: Mídia e Cotidiano Escolar”. Com base em Michel Foucault (1987) acerca do poder disciplinar presente na educação escolar e em Gilles Deleuze (1990) sobre as novas formas de controle em que participam a mídia, a pesquisa discute, em última instância, a função social da escola na contemporaneidade, problematizando a mídia como vetor de subjetivação no cotidiano escolar e no fazer pedagógico. No presente artigo colocamos em análise a metodologia da pesquisa, dando ênfase à centralidade do “diário de campo” e da“restituição”. Trabalharemos também o conceito de pesquisa-intervenção e sua implicação prática como estratégia da pesquisa com a escola. Discutiremos o uso do diário de campo como uma ferramenta facilitadora de vínculo e de construção de dados necessário para ressignificação da mesma. Destacaremos a restituição como o momento necessário para criarmos um campo de análise coletiva. Por fim, apresentaremos a importância dessas ferramentas para todo o processo de construção da nossa pesquisa.(AU)

Tinnitus Annoyance in Normal-Hearing Individuals: Correlation With Depression and Anxiety.

OBJECTIVE: To assess and correlate tinnitus annoyance in normal-hearing patients with auditory brainstem response and with anxiety/depression. METHODS: A sample of 84 individuals with tinnitus and normal hearing levels (pure-tone thresholds ≤25 dB HL) was compared to a matched control group of 47 normal-hearing individuals without tinnitus. All participants underwent auditory brainstem response testing. Tinnitus annoyance was assessed using the Tinnitus Handicap Inventory and depression and anxiety using the Beck Depression Inventory and Beck Anxiety Inventory, respectively. We compared auditory brainstem response and anxiety/depression symptoms between groups. In the study group, we correlated the degree of tinnitus annoyance with normal/abnormal auditory brainstem response and presence/level of anxiety/depression symptoms. RESULTS: All controls had normal auditory brainstem response; 30 patients with tinnitus had abnormal results. Thirty-five patients with tinnitus had depression and 41 anxiety, while only 2 controls had depression and none had anxiety, with a significant between-group difference (P < .001). Normal/abnormal auditory brainstem response showed no association with tinnitus annoyance, anxiety, or depression. A higher degree of tinnitus annoyance was associated with severity of depression and anxiety. CONCLUSIONS: Increased tinnitus annoyance was positively correlated with greater severity of anxiety and depression in normal-hearing patients but was unrelated to normal/abnormal auditory brainstem response.

Effects of a 2-Year Supervised Exercise Program Upon the Body Composition and Muscular Performance of HIV-Infected Patients.

BACKGROUND: There is a lack of research investigating long-term effects of exercise training upon the body composition and muscle function in HIV-infected patients (PHIV). The study investigated the influence of a 2-year supervised exercise program on body composition and strength of PHIV under highly active antiretroviral therapy (HAART). METHODS: A training program including aerobic, strength and flexibility exercises was performed by 27 PHIV (17 men/ 10 women; age: 48.7±7.0 years; HAART: 150.7±65.3 months) during 1 year and 18 PHIV (10 men/ 8 women; age: 50.6±5.2 years; HAART: 176.6±53.1 months) during 2 years. Body composition and knee isokinetic strength were assessed at baseline and at the end of each year of intervention. RESULTS: Body composition remained stable along the whole experiment vs baseline (1-year - total muscle mass: Δ men=1.1%, P=0.21; Δ women=1.4%, P=0.06; trunk fat: Δ men=-0.1%, P=0.65; Δ women=-1.5%, P=0.45; 2 years - total muscle mass: Δ men=2.7%, P=0.54; Δ women=-1.9%, P=0.71; trunk fat: Δ men=4.4%, P=0.96; Δ women=10.0%, P=0.30). After 1-year, peak torque increased in men (Δ extension=4.2%, P=0.01; Δ flexion=12.2%, P=0.04) and total work reduced in women (Δ extension=-15.4%, P=0.01, Δ flexion=-17.5%, P=0.05). All strength markers remained stable vs baseline after 2 years of intervention (P>0.05). Only men showed significant reduction in the risk of disability due to sarcopenia (P=0.05) after 1 year of intervention, which remained stable after 2 years. CONCLUSION: Long-term exercise training preserved strength and muscle mass in PHIV under HAART. Exercise programs should be part of HIV therapy to prevent sarcopenia of this population along the years. TRIAL REGISTRATION: ACTRN12610000683033; UTN U1111-1116-4416.

A method for determination of imazapic and imazethapyr residues in soil using an ultrasonic assisted extraction and LC-MS/MS.

At least 52 % of the planted rice area in Rio Grande do Sul, a major rice producing state in Brazil, employs Clearfield(®) production system, corresponding to 580,000 ha of cultivated area. To grow rice with Clearfield(®) technology, producers combine imazethapyr and imazapic herbicides. However, these herbicides leave residual activity in soil; consequently, the repeated application of imazethapyr and imazapic on Brazilian Clearfield(®) rice fields has increased these herbicides persistence in treated soils. In this study, a method has been developed for removal and quantification of imazethapyr and imazapic residues in soil through ultrasonic assisted extraction using methanol-phosphoric acid aqueous solution (pH 2.0). The detected response was linear for both herbicides within the range of 0.25-5 ng mL(-1) with correlations coefficients >0.99. The quantification limit was limit of quantification 0.2 µg Kg(-1) for both pesticides. The good recovery rate from all pesticides, which ranges between 70 % and 120 %, demonstrates the method's validity.

Is the degree of discomfort caused by tinnitus in normal-hearing individuals correlated with psychiatric disorders?

OBJECTIVE: To evaluate the annoyance of tinnitus in normal-hearing patients and to correlate it with outer hair cell function and with anxiety and depression disorders. STUDY DESIGN: Case-control study. SETTING: Tertiary care medical center. SUBJECTS AND METHODS: Sixty-eight patients with tinnitus (study group) and a control group consisting of 46 subjects without tinnitus were studied. The subjects ranged in age from 20 to 45 years and had a hearing threshold of up to 25 dB in the frequency range of 500 to 8000 Hz. The subjects were submitted to otoacoustic emission (OAE) tests. Tinnitus annoyance was evaluated using the Tinnitus Handicap Inventory, and anxiety and depression were measured using the Beck Anxiety and Depression Inventories. RESULTS: In the study group, 67% of the transient-evoked OAE tests were altered, with the observation of significant differences for all frequencies tested. In addition, 65.2% of the distortion product-evoked OAE tests were altered at 3000, 6000, and 8000 Hz, and this difference was significant when compared with control. Anxiety (44.1%) and depression (33.3%) were significantly more frequent among patients with tinnitus. Tinnitus annoyance was not correlated with the OAE results or tinnitus duration but showed a correlation with the presence of anxiety and depression. In the study group, no difference in tinnitus annoyance, anxiety, or depression was observed between patients with normal and altered OAE tests. CONCLUSION: This study showed altered OAE in patients with tinnitus and normal hearing. It also demonstrated a positive correlation between the annoyance of tinnitus and anxiety and depression in normal-hearing patients.

Effect of hormone replacement therapy on the auditory brainstem response of postmenopausal women.

OBJECTIVE: To investigate whether hormone replacement therapy modifies the auditory brainstem response in postmenopausal women. METHODS: Nineteen postmenopausal women received hormone replacement therapy (study group) and 25 received no treatment nor placebo (control group). In both groups, age ranged from 45 to 60 years and pure-tone sensitivity was 25 dB or better at frequencies between 500 and 2000 Hz. Auditory brainstem response was evaluated before and after 3 months of hormone use in the study group. The control group was also evaluated at the same periods. The following auditory brainstem response parameters were compared between the two groups: latencies of waves I, III, and V; I-III, III-V, and I-V interpeak intervals. RESULTS: Mean age did not differ between groups (study group: 51.5 ± 0.7 years; control: 52.9 ± 0.6 years). No significant differences in wave latencies, or interpeak intervals were observed between the two groups (p > 0.05).

Clinic-epidemiological analysis of an otorhinolaryngology emergency unit care in a tertiary hospital.

UNLABELLED: Emergencies are common in our Otorhinolaringology specialty. However, the clinical and epidemiological features are not very well known. OBJECTIVES: To evaluate the clinical and epidemiological profiles of otorhinolaryngological disorders in an emergency unit of a tertiary hospital, and to determine the appropriateness of the level of health care for a tertiary hospital. MATERIALS AND METHODS: An analytical study using data records of an otorhinolaryngological emergency unit at a tertiary hospital in the Federal District for a year, full time, and no screening. The age, sex, arrival time and clinical diagnosis were evaluated. The entities were separated into cases of pharingolaryngoesthomatology, otology, rhinology, and head and neck surgery. These were evaluated according to the urgency level, the required care, and the arrival time. RESULTS: 26,584 data records were selected, of which 2,001 were excluded. The group comprised 54. 48% women, and 45. 51% men. Otological complaints (62. 27%) prevailed. 61. 26% of cases were considered emergencies. Only 9. 7% of those required medium or high complex resources for resolution. CONCLUSIONS: The study showed that 61. 26% of the otorhinolaryngological cases are emergencies, and only 9. 7% required medium or high complexity resources.

Clinic-epidemiological analysis of an otorhinolaryngology emergency unit care in a tertiary hospital / Análise clínico-epidemiológica do atendimento de pronto-socorro de otorrinolaringologia de um hospital terciário

Emergencies are common in our Otorhinolaringology specialty. However, the clinical and epidemiological features are not very well known. OBJECTIVES: To evaluate the clinical and epidemiological profiles of otorhinolaryngological disorders in an emergency unit of a tertiary hospital, and to determine the appropriateness of the level of health care for a tertiary hospital. MATERIALS AND METHODS: An analytical study using data records of an otorhinolaryngological emergency unit at a tertiary hospital in the Federal District for a year, full time, and no screening. The age, sex, arrival time and clinical diagnosis were evaluated. The entities were separated into cases of pharingolaryngoesthomatology, otology, rhinology, and head and neck surgery. These were evaluated according to the urgency level, the required care, and the arrival time. RESULTS: 26,584 data records were selected, of which 2,001 were excluded. The group comprised 54. 48 percent women, and 45. 51 percent men. Otological complaints (62. 27 percent) prevailed. 61. 26 percent of cases were considered emergencies. Only 9. 7 percent of those required medium or high complex resources for resolution. CONCLUSIONS: The study showed that 61. 26 percent of the otorhinolaryngological cases are emergencies, and only 9. 7 percent required medium or high complexity resources.

Pronto-atendimento em Otorrinolaringologia é uma prática constante da especialidade. Entretanto, as características clínico-epidemiológicas são pouco conhecidas. OBJETIVOS: Avaliar fatores clínico-epidemiológicos referentes às doenças otorrinolaringológicas de pacientes atendidos no pronto-socorro de um hospital terciário e avaliar a adequação do nível de atenção em saúde em relação ao atendimento prestado em um hospital terciário. MATERIAIS E MÉTODOS: Estudo analítico, transversal a partir dos dados de guias de atendimentos do pronto socorro de otorrinolaringologia de um hospital terciário do Distrito Federal durante 01 ano, em horário integral, sem triagem. Foram avaliados: idade, sexo, horário de chegada e diagnóstico clínico. Os eventos foram divididos em otologia, rinologia, faringolaringoestomatologia e cirurgia de cabeça e pescoço. Os eventos foram avaliados segundo o grau de urgência, nível de assistência necessário e o horário de chegada. RESULTADOS: Foram colhidas 26. 584 guias de atendimentos, sendo excluídas 2001. 54,48 por cento eram mulheres e (45,51 por cento) homens. Queixas otológicas foram as mais prevalentes (62,27 por cento). Foram consideradas urgências 61,26 por cento dos atendimentos; apenas 9,7 por cento atendimentos necessitaram de recursos de média ou alta complexidade. CONCLUSÃO: O estudo mostrou que 61,26 por cento das doenças otorrinolaringológicas atendidas no pronto-socorro são urgências e que apenas 9,7 por cento necessitam de recursos de média ou alta complexidade.

Neurilemoma de língua / Lingual neurilemmoma

O neurilemoma é um tumor benigno das células da bainha de Schwann. Quando é extracraniano, afetam as regiõesda cabeça e do pescoço em 25% dos casos, sendo a boca um sítio comum de lesão. É um tumor encapsulado,de crescimento vagaroso, cujo tratamento é excisão completa. O diagnóstico é histopatológico. Descreve-se o casode uma paciente do sexo feminino, 23 anos, não tabagista, com lesão pequena pedunculada no terço posterior dalíngua à direita. Submetida à biopsia excisional com anestesia local, em ambulatório, o diagnóstico foi neurilemoma.O estudo de imagem após excisada a lesão, no acompanhamento clínico, mostrou que a lesão foi completamenteremovida. A paciente encontra-se assintomática no momento. Mesmo raro, o neurilemoma deve ser considerado nodiagnóstico diferencial das lesões tumorais orais.

The neurilemmoma is a benign tumor of the Schwann cells. It affects the head and neck in 25% of the extracranialneurilemmomas, being the mouth the most common site. It is an encapsulated tumor with slow growing. The treatmentis complete excision. A 23-year-old woman, no-smoking is described. She had a small, pedunculated mass inthe posterior third of the tongue, at the right side. Its diagnosis is histopathologic. The ressection of the pedunculatedlesion were performed with local anesthesia. The histopathological diagnosis was neurilemmoma. In the follow up, theradiology exam demonstrated complete excision and the patient is assintomatic. Although rare, the neurilemmomashould be considered in the differencial diagnosis of oral tumor lesions.

Fatores de risco para hipertensão arterial: investigação em motoristas e cobradores de ônibus / Risk factors to high blood pressure: inquiry with bus drivers and collectors

Estudo transversal com objetivo de investigar fatores de risco cardiovascular em motoristas e cobradores de ônibus e analisar a relação dos valores de pressão arterial e glicemia com demais fatores de risco. Realizado em Fortaleza–Ceará, em 2005, mediante entrevista e avaliação antropométrica, glicêmica e medida da pressão arterial com 124 motoristas e 96 cobradores. Verificou-se valores da pressão arterial limítrofes ou alterados em 49,2; 72,9 com sobrepeso e obesidade; 87,3 sedentários; 53,4 ingeriam bebida alcoólica; e 73,6 consumiam gordura animal. Encontrou-se correlação estatisticamente relevante entre consumo de gordura animal e valores médios elevados de pressão arterial sistólica e entre consumo reduzido do sal, glicemia e pressão arterial diastólica. Isso sugere dificuldade para avaliar este aspecto a partir da informação do participante. Evidenciou-se presença de significativos fatores de risco cardiovascular, o que indica a necessidade da implementação de ações educativas visando especialmente o desenvolvimento de atividades físicas e alimentação adequada.

This cross-sectional study aims at both investigating cardiovascular risk factors to workers from an urban transport company and analyzing the relation of blood pressure values and glucose with other risk factors. It was carried out in Fortaleza-Ceará, Brazil, in 2005, by means of interviews and anthropometric evaluation, glucose evaluation, and blood pressure measurements with 124 drivers and 96 collectors. Results demonstrated that 49.2 had borderline or modified blood pressure values; 72.9 were overweight and obese; 87.3 were sedentary; 53.4 ingested alcohol; and 73.6 ate animal fat. There was significant statistic correlation between consumption of animal fat and increased mean values of systolic blood pressure as well as between reduced consumption of salt, and glucose and diastolic blood pressure. This specific aspect is difficult to evaluate on the basis of the information provided by the participant. As significant cardiovascular risk factors were evinced, implementation of educative actions is suggested, aiming at the development of physical activities and proper dieting.

Estudio transversal con objetivo de investigar los factores de riesgo cardiovascular en trabajadores de autobús y analizar la relación de los valores de la presión arterial y glucemia con los demás factores de riesgo. Desarrollado en Fortaleza-Ceará-Brasil, en 2005, mediante entrevista y evaluación antropométrica, de la glucemia y medida de la presión arterial con 124 motoristas y 96 cobradores. Los resultados demostraron que 49,2 presentaba valores de presión sanguínea límites o alterados; 72,9 tenían sobrepeso y obesidad; 87,3 no desarrollaban actividad física; 53,4 ingerían bebida alcohólica; y 73,6 consumían grasa animal. Fue encontrada correlación estadística significante entre el consumo de grasa animal y los valores medios elevados de presión arterial sistólica y entre consumo reducido de sal, glucemia y presión arterial diastólica. Eso sugiere dificultad para evaluar este aspecto a partir de la información del participante. Se evidenció que hay significativos factores de riesgo cardiovascular, que indican la necesidad de actividades educativas que planteen  especialmente el desarrollo de actividades físicas y alimentación adecuada.

Modo fisiológico do modelo de adaptação de Sister Callista Roy: análise reflexiva segundo Meleis / Physiologic mode of Sister Callista Roy adaptation model: reflexive analysis according to Meleis

Although of the science proclaimed evolution and, consequently, of the nursing, still today we realize certain difficulty by nurses in work with theories, so much in the assistance scope, how much in the of the teaching or research. With sights to improvement of this difficulty, we highlight the application importance of theoretical landmarks for a better knowledge construction in nursing, in intention of deepening the rendered assistance. We aimed to analyze the consistency and clearness of the physiologic mode presented in the adaptation theory of Sister Callista Roy in the master dissertation. For the critical analysis of the theory, we opt by the theory analysis model of Meleis (1997). We opt for working with the theory criticism, considering the clearness and consistency criteria. The adaptation model utilization, It propose for Sister Callista Roy, presented validity and aplicability in the study at issue, once it enabled the determination of important aspects for the nursing assistance the women with angina pectoris, considering them like a biopsychosocial, adaptive and holistic being. The theories analysis model of Meleis, it contributed of significant form for the proposed goal, enabling a vision enlarged of the theory of Sister Callista Roy, elucidating her importance of the same for the nursing development, in the teaching, research and assistance scopes.

Apesar da proclamada evolução da ciência e, conseqüentemente, da enfermagem, ainda hoje percebemos certa dificuldade por parte do enfermeiro em trabalhar com teorias, tanto no âmbito da assistência, quanto no do ensino ou pesquisa. Com vistas à superação desta dificuldade, destacamos a importância da aplicação de marcos teóricos para uma melhor construção do conhecimento em enfermagem, no intuito de aprofundar a assistência prestada. Objetivamos analisar a consistência e a clareza do modo fisiológico apresentado na teoria de adaptação de Sister Callista Roy em uma dissertação de mestrado. Para a análise crítica da teoria, optamos pelo modelo de análise de teoria de Meleis (1997) e trabalhamos com a crítica da teoria, considerando os critérios de clareza e consistência. A utilização do modelo de adaptação proposto por Sister Callista Roy mostrou validade e aplicabilidade no estudo em discussão, uma vez que possibilitou a determinação de aspectos relevantes para a assistência de enfermagem a mulheres com angina pectoris, e propiciou percebê-las como um ser biopsicossocial, adaptativo e holístico. De forma significativa, o modelo de análise de teorias de Meleis contribuiu para o objetivo proposto, ao possibilitar uma visão ampliada da teoria de Sister Callista Roy, e explicitou a importância desta para o desenvolvimento da enfermagem nos âmbitos do ensino, pesquisa e assistência.

Linguagens da sistematização da assistência de enfermagem nas dissertações e teses dos catálogos do centro de estudo e pesquisa em enfermagem / Languages of the nursing process in the dissertations and theses: a bibliographical study

The objective of this bibliographical study was to analyze trends of use of the nursing assistance systematization languages in the dissertations and theses published in the catalogs of the Center of Study and Research in Nursing, in the period from 1979 to 2004. The sample was composed of 98 abstracts that constituted research about assistance systemization or that contemplated theories with own language to a nursing process. It was observed: The concentration of studies in the Southeast and Northeast regions; The utilization of the Nanda as nursing language; The application of the Basic Human Needs Theory; The development of studies about profile and validation of diagnosis. The trends of utilization of the founded languages in this study, as well as the evidenced gaps show the need of better knowledge and comprehension to nursing process elements that give base to the classifications at its utilization in the practice, in the research and in the teaching.

O estudo teve como objetivo analisar as tendências da utilização das linguagens da sistematização da assistência de enfermagem nas dissertações e teses publicadas nos catálogos do Centro de Estudo e Pesquisa em Enfermagem (CEPEn), no período de 1979 a 2004. Estudo do tipo bibliográfico, desenvolvido entre novembro de 2005 e fevereiro de 2006. Foram analisados 98 resumos que constituíam trabalhos de pesquisa relacionados à sistematização da assistência ou que contemplavam teorias com linguagem própria, vinculada a um processo de enfermagem. Observaram-se como tendências: concentração de estudos nas regiões Sudeste e Nordeste; utilização da NANDA como linguagem de enfermagem; aplicação da Teoria das Necessidades Humanas Básicas; desenvolvimento de estudos de perfil e validação de diagnósticos. As tendências de utilização das linguagens encontradas neste estudo, bem como as lacunas evidenciadas apontam para a necessidade de melhor conhecimento e compreensão dos elementos do processo de enfermagem que dão base às classificações para sua utilização na prática, na pesquisa e no ensino.