Enhancing motor functional recovery in spinal cord injury through pharmacological inhibition of Dickkopf-1 with BHQ880 antibody.

BACKGROUND: Mounting experimental evidence has underscored the remarkable role played by the Wnt family of proteins in the spinal cord functioning and therapeutic potential in spinal cord injury (SCI). We aim to provide a therapeutic prospect associated with the modulation of canonical Wnt signaling, examining the spatio-temporal expression pattern of Dickkopf-1 (Dkk1) and its neutralization after SCI. We employ an intraparenchymal injection of the clinically validated Dkk1-blocking antibody, BHQ880, to elucidate its effects in SCI. METHODS: A rat model of contusion SCI was used. Histological analyses were performed, wherein Dkk1 protein was sought, and ELISA analyses were employed for Dkk1 detection in cerebrospinal fluid and serum. To ascertain the BHQ880 therapeutic effect, rats were subjected to SCI and then injected with the antibody in the lesion epicenter 24 hours post-injury (hpi). Subsequent evaluation of motor functional recovery extended up to 56 days post-injury (dpi). qRT-PCR and histological analyses were conducted. RESULTS: We demonstrate the presence of Dkk1 in the healthy rat spinal cord, with pronounced alterations observed following injury, primarily concentrated in the epicenter regions. Notably, a significative upregulation of Dkk1 was detected at 24 hpi, peaking at 3 dpi and remaining elevated until 42 dpi. Moreover, we revealed that early administration of BHQ880 considerably improved motor functional recovery, promoted preservation of myelinated tissue, and reduced astroglial and microglia/macrophage reactivity. Furthermore, there was a decrease in the acute expression of different inflammatory genes. CONCLUSIONS: Collectively, our findings highlight the therapeutic potential of BHQ880 treatment in the context of SCI.

Key Factors in Crane-Related Occupational Accidents in the Spanish Construction Industry (2012-2021).

The construction industry is one of the riskiest sectors worldwide, with crane operations being one of the most dangerous activities. The aim of this study was to gain insight into the key factors involved in crane-related occupational accidents in the construction industry in Spain. To this end, 1314 accidents involving cranes were analyzed from a total of 241,937 accidents that occurred in the construction of buildings. The data were collected from the Spanish government's occupational accident statistics corresponding to the years 2012-2021. The results evidenced a statistically significant relationship between cranes as the material agent and the size of the company, with 95% of cases corresponding to small- or medium-sized companies (less than 250 employees). Additionally, it shows how the crane operator is identified as a material contributor to crane accidents in the construction industry, and may be considered a key component to these accidents. In conclusion, improving the knowledge gained about the key factors in crane-related accidents at work in the construction industry provides essential information that helps to design and implement appropriate preventive measures to avoid the recurrence of unwanted events with these machines.

La soledad en el cine a propósito de Lost in translation (2003) y Her (2013) / Loneliness in Cinema Regarding Lost in Translation(2003) andHer (2013)

Este artículo analiza el concepto de soledad a través de un enfoque narrativo. Para ello se comparan las películas Lost in Translation de Sofía Coppola y Her de Spike Jonze. En ambas historias es posible apreciar a personajes acosados por la soledad. Este trabajo se apoya, por un lado, en la teoría de Robert Weiss sobre la soledad y, por otro lado, en la metodología empleada por Donio-Bellgade et al. quienes diseñaron una serie de categorías de soledad para analizar películas. En este estudio se han empleado las siguientes: 1) elección de la vida en solitario por decisión propia, 2) sentirse solo viviendo acompañado, 3) falta de apoyo social y 4) depresión e ideas suicidas. A partir de estas categorías de la soledad se analiza lo que sucede a los protagonistas de los dos filmes. (AU)

This article analyzes the concept of loneliness through a narrative approach. For this purpose, the films Lost in Translation by Sofía Coppola and Her by Spike Jonze are compared. In both stories, characters harassed by loneliness can be seen. This work is supported, on the one hand, by Robert Weiss' theory on loneliness and, on the other, the methodology used by Donio-Bellgade et al. who designed a series of loneliness categories to analyze films. In this study the following have been used: 1) choosing to live alone by choice, 2) feeling alone living with others, 3) lack of social support and 4) depression and suicidal ideas. Based on these categories of loneliness, we analyze what happens to the protagonists of the two films. (AU)

HEMATOLOGIC AND BIOCHEMICAL VALUES OF FREE-RANGING HAWKSBILL SEA TURTLES (ERETMOCHELYS IMBRICATA) IN GLOVER'S REEF, BELIZE.

Blood samples were obtained from the dorsal cervical sinus of free-ranging hawksbill sea turtles (Eretmochelys imbricata) collected at Glover's Reef Marine Reserve, 42 km east of the coast of Belize, for hematology and plasma biochemistry analysis. Unknown sex, subadult turtles (N = 32) were sampled in 2013 (n = 22) and 2017 (n = 10). To provide a more robust data set, parameters that did not have statistically significant differences were pooled and treated as a single population. Eleven hematologic parameters were evaluated; of these, five were pooled. Twenty-three plasma biochemical parameters were evaluated; of these, 15 were pooled. The PCV observed in this study (mean 33.44%) was double that observed in two studies of juvenile hawksbills in Dubai (means 17% and 16%), whereas the total WBC count was half that observed in immature and adult hawksbills sampled in the Galápagos (mean 2.91 × 103 versus 5.3 × 103/µl). Total protein and albumin were lower than regionally similar, adult female hawksbills in Brazil (means 3.36 versus 5.45 g/dl and 0.93 versus 2.11 g/dl, respectively). Globulins were higher (mean 2.43 versus 1.06 and 0.5 g/dl), driving the albumin:globulin ratio lower than that observed in two studies of juvenile hawksbills in Dubai (0.4:1 versus 1.1:1 and 1:1, respectively). These findings represent a geographically distinct population from previous reports, highlight the variability in blood parameters from disparate populations, and reaffirm the vital importance of considering a multitude of variables when interpreting reptilian blood values. The similarities in the majority of values observed in 2013 and 2017 provide confidence in the stability of these parameters in this population.

Belize Blue Carbon: Establishing a national carbon stock estimate for mangrove ecosystems.

Mangrove ecosystems are among the most economically and ecologically valuable marine environments in the world. Mangroves are effective at long-term carbon storage within their sediments and are estimated to hold 12 billion metric tons of carbon worldwide. These ecosystems are therefore vitally important for carbon sequestration and, by extension, climate change mitigation. As part of the Paris Agreement, participating countries agree to provide plans to reduce their carbon emissions, or nationally determined contributions (NDCs). However, despite mangroves being recognized as important nature-based solutions, many countries still lack national data on carbon stocks and must use global or regional averages, which may not be sufficiently accurate. Here, we present the national carbon stock estimate of mangrove ecosystems for the NDC of Belize, acquired through a collaborative approach involving government agencies and NGOs. We conducted a comprehensive sampling of mangroves across the country, including a range of mangrove ecotypes. The mean total ecosystem carbon stock (TECS) for the nation was 444.1 ± 21.0 Mg C ha-1, with 74.4 ± 6.2 Mg C ha-1 in biomass stocks, and 369.7 ± 17.7 Mg C ha-1 in sediment stocks. Combining these data with a recent mapping effort, we provide the first national comprehensive mangrove carbon stock estimate of 25.7 Tg C. The national mean from this study varies from previous global analyses, which can under- or overestimate TECS by as much as 0.6 Tg C and 16.5 Tg C, respectively, depending on the study. These data supported the NDC update of Belize, and can be used to inform the country's mangrove protection and restoration commitments. The collaborative approach of this work should serve as a blueprint for other countries seeking to conserve natural blue carbon sinks as a strategy to achieve their climate targets.

Humoral response after the fourth dose of the SARS-CoV-2 vaccine in the CKD spectrum: a prespecified analysis of the SENCOVAC study.

BACKGROUND: There is scarce evidence on the fourth dose of severe acute respiratory syndrome coronavirus 2 vaccines in chronic kidney disease (CKD) patients. We evaluated the humoral response and effectivity of the fourth dose in the CKD spectrum: non-dialysis CKD (ND-CKD), haemodialysis (HD), peritoneal dialysis (PD) and kidney transplant (KT) recipients. METHODS: This is a prespecified analysis of the prospective, observational, multicentric SENCOVAC study. In patients with CKD who had received a complete initial vaccination and one or two boosters and had anti-Spike antibody determinations 6 and 12 months after the initial vaccination, we analysed factors associated with persistent negative humoral response and higher anti-Spike antibody titres as well as the efficacy of vaccination on coronavirus disease 2019 (COVID-19) severity. RESULTS: Of 2186 patients (18% KT, 8% PD, 69% HD and 5% ND-CKD), 30% had received a fourth dose. The fourth dose increased anti-Spike antibody titres in HD (P = .001) and ND-CKD (P = .014) patients and seroconverted 72% of previously negative patients. Higher anti-Spike antibody titres at 12 months were independently associated with repeated exposure to antigen (fourth dose, previous breakthrough infections), previous anti-Spike antibody titres and not being a KT recipient. Breakthrough COVID-19 was registered in 137 (6%) patients, 5% of whom required admission. Admitted patients had prior titres <620 UI/ml and median values were lower (P = .020) than in non-admitted patients. CONCLUSIONS: A fourth vaccine dose increased anti-Spike antibody titres or seroconverted many CKD patients, but those with the highest need for a vaccine booster (i.e. those with lower pre-booster antibody titres or KT recipients) derived the least benefit in terms of antibody titres. Admission for breakthrough COVID-19 was associated with low anti-Spike antibody titres.

Silk-Elastin-like Polymers for Acute Intraparenchymal Treatment of the Traumatically Injured Spinal Cord: A First Systematic Experimental Approach.

Despite the promising potential of hydrogel-based therapeutic approaches for spinal cord injury (SCI), the need for new biomaterials to design effective strategies for SCI treatment and the outstanding properties of silk-elastin-like polymers (SELP), the potential use of SELPs in SCI is currently unknown. In this context, we assessed the effects elicited by the in vivo acute intraparenchymal injection of an SELP named (EIS)2-RGD6 in a clinically relevant model of SCI. After optimization of the injection system, the distribution, structure, biodegradability, and cell infiltration capacity of (EIS)2-RGD6 were assessed. Finally, the effects exerted by the (EIS)2-RGD6 injection-in terms of motor function, myelin preservation, astroglial and microglia/macrophage reactivity, and fibrosis-were evaluated. We found that (EIS)2-RGD6 can be acutely injected in the lesioned spinal cord without inducing further damage, showing a widespread distribution covering all lesioned areas with a single injection and facilitating the formation of a slow-degrading porous scaffold at the lesion site that allows for the infiltration and/or proliferation of endogenous cells with no signs of collapse and without inducing further microglial and astroglial reactivity, as well as even reducing SCI-associated fibrosis. Altogether, these observations suggest that (EIS)2-RGD6-and, by extension, SELPs-could be promising polymers for the design of therapeutic strategies for SCI treatment.

Assessment of Quantiferon®-CMV and Immuknow® Assays in CMV-seropositive Lung Transplant Recipients to Stratify Risk of CMV Infection

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Factors Involved in Endothelial Dysfunction Related to Angiogenic Disbalance and Oxidative Stress, in Women at High Risk of Term Pre-Eclampsia.

Oxidative and inflammatory stress, angiogenic imbalance, and endothelial dysfunction are pathophysiological mechanisms occurring in pre-eclampsia (PE) that may persist over time and predispose women to a higher risk of cardiovascular disease (CVD) in the future. However, there is little evidence on the vascular function of women at risk of PE who have not developed the disease. The main objective of this research is to study factors and biomarkers involved in endothelial dysfunction related to oxidative stress, angiogenic disbalance, and inflammation in women at high risk of term PE who do not develop the disease. An observational, analytical, retrospective, and descriptive study was carried out in a selected sample of 68 high-risk and 57 non-risk of term PE participants in the STATIN study (FFIS/2016/02/ST EUDRACT No: 2016-005206-19). A significant increase in mean arterial pressure (MAP) levels and oxidative stress biomarkers (uric acid, homocysteine, and total serum antioxidant capacity) was found. Biomarkers of inflammation (interleukin-6 and growth differentiation factor 15) and endothelial function (asymmetric dimethylarginine) were significantly elevated in the group at risk of pre-eclampsia. A significative dependence relationship was also established between MAP and interleukin-6 and uric acid. These results suggest that women at high risk of term PE may represent pregnancies with pre-existing maternal risk factors for CVD, manifested by the own cardiovascular overload of pregnancy. A better understanding of maternal cardiovascular function in pregnancy would allow the improved prediction of CVD late in life in women.

Development and Characterization of pH-Dependent Cellulose Acetate Phthalate Nanofibers by Electrospinning Technique.

The aim of this work was to obtain pH-dependent nanofibers with an electrospinning technique as a novel controlled release system for the treatment of periodontal disease (PD). Cellulose acetate phthalate (CAP) was selected as a pH-sensitive and antimicrobial polymer. The NF was optimized according to polymeric dispersion variables, polymer, and drug concentration, and characterized considering morphology, diameter, entrapment efficiency (EE), process efficiency (PE), thermal properties, and release profiles. Two solvent mixtures were tested, and CHX-CAP-NF prepared with acetone/ethanol at 12% w/v of the polymer showed a diameter size of 934 nm, a uniform morphology with 42% of EE, and 55% of PE. Meanwhile, CHX-CAP-NF prepared with acetone/methanol at 11% w/v of polymer had a diameter of 257 nm, discontinuous nanofiber morphology with 32% of EE, and 40% of PE. EE and PE were dependent on the polymer concentration and the drug used in the formulation. Studies of differential scanning calorimetry (DSC) showed that the drug was dispersed in the NF matrix. The release profiles of CHX from CHX-CAP-NF followed Fickian diffusion dependent on time (t0.43-0.45), suggesting a diffusion-erosion process and a matrix behavior. The NF developed could be employed as a novel drug delivery system in PD.

Generaciones centenial y milenial: actitudes hacia la sexualidad / The Centennial and Millennial generations: Attitudes towards sexuality / Gerações centennial e millennial: atitudes em relação à sexualidade

Resumen (analítico) El objetivo general fue conocer las actitudes hacia la sexualidad de estudiantes universitarios de las generaciones milenial y centenial. Como objetivo específico se validó un cuestionario de actitudes. Se siguió una metodología no experimental, transversal, con análisis descriptivo de respuestas de 402 estudiantes (69.1% mujeres, 30.9% hombres), con una media de edad de 20.8 años (DE 2.07). Resultaron actitudes favorables hacia: expresiones sexuales homosexuales y transexuales, eliminación de roles de género, uso del condón y re-chazo hacia la violencia en redes sociales. Se presentaron actitudes desfavorables hacia el amor y actitudes indecisas hacia las relaciones casuales. Concluimos que el conocimiento de las actitudes es fundamental en el diseño y la ejecución de políticas de salud pública dirigidas a promover y preservar la salud sexual de las generaciones actuales.

Abstract (analytical) The general objective of this study was to identify attitudes towards sexuality held by university students pertaining to the millennial and centennial generations. An attitudes questionnaire was validated as a specific objective. A non-experimental, cross-sectional methodology was followed, involving a descriptive analysis of 402 students' responses (69.1% women, 30.9% men) with a mean age of 20.8 years (SD 2.07). Favorable attitudes were found towards: homosexual and transexual expressions of sexuality, elimination of gender roles, condom use and rejection of violence in social networks. There were unfavorable attitudes toward love and marriage and a lack of definition towards casual relationships. The authors conclude that knowledge of attitudes is essential for the design and implementation of public health policies aimed at promoting and preserving the sexual health of current generations.

Resumo (analítico) O objetivo geral foi conhecer as atitudes face à sexualidade de estudantes universitários das gerações millennial e centennial, tendo como objetivo específico validar um questionário de atitudes. Foi seguida uma metodologia não experimental, transversal, com análise descritiva das respostas de 402 alunos (69.1% mulheres, 30.9 % homens), com uma média de idade de 20.8 anos (DP 2.07). Atitudes favoráveis foram encontradas em relação a: expressões sexuais homossexuais e transexuais, eliminação de papéis de gênero, uso de preservativo e repulsa face à violência nas redes sociais. Simultaneamente, atitudes desfavoráveis foram encontradas em relação ao amor e ao casamento e atitudes indefinidas em relação aos relacionamentos casuais. Concluímos que o conhecimento das atitudes é essencial na formulação e implementação de políticas públicas de saúde dirigidas a promover e preservar a saúde sexual das gerações atuais.

Propiedades psicométricas del cuestionario de estrés académico en la universidad (CEAU) en una muestra de estudiantes de Medicina mexicanos / Psychometric properties of the University Academic Stress Questionnaire (CEAU) in a sample of Mexican medical students

Introducción: El estrés se asocia a un pobre desempeño académico y problemas de salud mental entre los universitarios. Existen pocos instrumentos adaptados a esta población que identifiquen las fuentes de estrés académico. Objetivo: Evaluar las propiedades psicométricas del cuestionario de estrés académico en la universidad (CEAU) en estudiantes mexicanos. Sujetos y métodos: Contestaron el CEAU y la escala de malestar psicológico de Kessler (K-10) 232 alumnos de primer año de Medicina. El CEAU contiene 19 ítems sobre situaciones escolares potencialmente estresantes, mientras que la K-10 mide el malestar psicológico global inespecífico con 10 ítems. La validez de constructo se evaluó por composición factorial y por convergencia con la K-10. Las medidas de confiabilidad se obtuvieron por consistencia interna y estabilidad temporal. Resultados: Los reactivos se agruparon en los cuatro componentes de su versión original y explicaron el 45% de la varianza total, con cargas factoriales bajas en dos reactivos y uno fuera del factor propuesto inicialmente; eliminar este último reactivo produjo un mejor ajuste de los componentes y aumentó la explicación de la varianza al 57%. La consistencia interna fue de alfa = 0,88 para la puntuación total, con valores de alfa = 0,70-0,83 en sus subescalas y una estabilidad temporal de r(202) = 0,76 (p = 0,0001). La correlación del CEAU con la K-10 fue de r(232) = 0,64 (p = 0,0001). Conclusiones: El CEAU cumple con los criterios de confiabilidad y validez de constructo, se ajusta a los factores propuestos y correlaciona con el malestar psicológico en el sentido esperado.(AU)

Introduction: Stress is associated with poor academic performance and mental health problems among university students. There are few instruments adapted to this population that identify sources of academic stress. Objective: To evaluate the psychometric properties of the Academic Stress Questionnaire at University (CEAU) in Mexican students. Subjects and methods: 232 first-year medical students answered the CEAU and the Kessler psychological distress scale (K-10). The CEAU contains 19 items on potentially stressful school situations, while the K-10 measures nonspecific global psychological distress with 10 items. The construct validity was evaluated by factorial composition and by convergence with the K-10. Reliability measures were obtained by internal consistency and temporal stability. Results: The items were grouped into the four components of their original version and explained 45% of the total variance, with low factor loadings in two items and one outside the initially proposed factor; eliminating this last item produced a better fit of the components and increased the explanation of the variance to 57%. The internal consistency was α = 0.88 for the total score, with values from α = 0.70 to 0.83 in its subscales and a temporal stability of r(202) = 0.76 (p = .0001). The correlation between CEAU and K-10 was r(232) = 0.64 (p = .0001). Conclusions: The CEAU meets the reliability and construct validity criteria, adjusts to the proposed factors and correlates with psychological distress in the expected sense.(AU)

Predictive Value of Immune Cell Functional Assay for Non-Cytomegalovirus Infection in Lung Transplant Recipients: A Multicenter Prospective Observational Study / Valor predictivo del test de función de la inmunidad celular para infecciones distintas a CMV en receptores de un trasplante pulmonar: un estudio multicéntrico, prospectivo y observacional

Introduction: Immune cell functional assay (ImmuKnow®) is a non-invasive method that measures the state of cellular immunity in immunosuppressed patients. We studied the prognostic value of the assay for predicting non-cytomegalovirus (CMV) infections in lung transplant recipients. Methods: A multicenter prospective observational study of 92 patients followed up from 6 to 12 months after transplantation was performed. Immune cell functional assay was carried out at 6, 8, 10, and 12 months. Results: Twenty-three patients (25%) developed 29 non-CMV infections between 6 and 12 months post-transplant. At 6 months, the immune response was moderate (ATP 225–525ng/mL) in 14 (15.2%) patients and low (ATP<225ng/mL) in 78 (84.8%); no patients had a strong response (ATP≥525ng/mL). Only 1 of 14 (7.1%) patients with a moderate response developed non-CMV infection in the following 6 months compared with 22 of 78 (28.2%) patients with low response, indicating sensitivity of 95.7%, specificity of 18.8%, positive predictive value (PPV) of 28.2%, and negative predictive value (NPV) of 92.9% (AUC 0.64; p=0.043). Similar acute rejection rates were recorded in patients with mean ATP≥225 vs. <225ng/mL during the study period (7.1% vs. 9.1%, p=0.81). Conclusion: Although ImmuKnow® does not seem useful to predict non-CMV infection, it could identify patients with a very low risk and help us define a target for an optimal immunosuppression. (AU)

Introducción: El test de función de la inmunidad celular (ImmuKnow®) es un método que mide el estado de la inmunidad celular en pacientes inmunosuprimidos. Se estudió su valor pronóstico para predecir infecciones diferentes a citomegalovirus (CMV) en receptores de un trasplante pulmonar. Métodos: Se realizó un estudio observacional prospectivo multicéntrico de 92 pacientes seguidos desde los 6 a los 12 meses postrasplante. El test se realizó a los 6, 8, 10 y 12 meses. Resultados: Veintitrés pacientes (25%) desarrollaron 29 infecciones no debidas a CMV entre los 6 y los 12 meses posteriores al trasplante. A los 6 meses, la respuesta inmune fue moderada (ATP 225-525ng/ml) en 14 (15,2%) pacientes y baja (ATP<225ng/ml) en 78 (84,8%); ningún paciente tuvo una respuesta fuerte (ATP>525ng/ml). Solo uno de 14 (7,1%) pacientes con una respuesta moderada desarrolló una infección diferente a CMV en los 6 meses siguientes a la realización del test en comparación con 22 de 78 (28,2%) con respuesta baja, indicando una sensibilidad del 95,7%, una especificidad del 18,8%, un valor predictivo positivo del 28,2% y un valor predictivo negativo del 92,9% (AUC 0,64; p=0,043). Se registraron tasas de rechazo agudo similares en pacientes con ATP medio >225 frente a <225ng/ml durante el período de estudio (7,1 frente al 9,1%; p=0,81). Conclusión: Aunque el test ImmuKnow® no parece útil para predecir infecciones diferentes al CMV, podría identificar pacientes con riesgo muy bajo y ayudarnos a definir un objetivo de inmunosupresión óptima. (AU)