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Reprod Biomed Online ; 14(1): 96-101, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17207341

ABSTRACT

Two-cell murine embryos were cultured for 72 h in the presence or absence of granulocyte-macrophage colony stimulating factor (GM-CSF), frozen for 60 days and, after thawing, cultured for an additional 24 h in the presence or absence of GM-CSF. During the initial 72 h period, GM-CSF did not influence the percentage of embryos reaching the expanded blastocyst stage, but there was a significant increase (P < 0.05) in the number of cells in the embryos grown with GM-CSF. Survival after thawing was not affected by previous exposure to GM-CSF, but re-expansion of the blastocoele was diminished in that group. Exposure to GM-CSF during the post-thaw period greatly enhanced re-expansion of the blastocoele. The presence of human serum albumin in the culture media is thought to have masked the beneficial effect of GM-CSF upon embryos.


Subject(s)
Blastocyst/drug effects , Cryopreservation , Embryonic Development/drug effects , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Animals , Blastocyst/cytology , Blastocyst/physiology , Cell Proliferation/drug effects , Culture Media , Female , Humans , Mice , Serum Albumin
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