Bone marrow trephine biopsy involvement by lymphoma: pattern of involvement and concordance with flow cytometry, in 10 years from a single institution

Purpose: To evaluate the features of bone marrow (BM) biopsy involvement by lymphoma, pattern of infiltration, morphological analysis and flow cytometry were reviewed at all lymphoma patients over a period of 10 years. Methods/patients: 413 cases were included in the study if BM biopsy slides were available. Only 356 patients had both BM trephine biopsy and flow cytometry. Results: The most frequent subtype was diffuse large B cell (31.2 %), followed by follicular lymphoma (18.9 %). The predominant pattern was mixed (nodular-interstitial) (9.2 %). Morphological marrow infiltration was found in 138 cases, and flow cytometry identified 117 cases with BM involvement. A concordance between the two methods was detected in 305 cases (85.7 %). There was discordance in 51 cases (14.3 %): morphology positive/FC negative in 33 cases and morphology negative/FC positive in 18. Conclusions: Flow cytometry is slightly more useful in detecting involvement when the BM is affected, but this finding is not conclusive (AU)

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Bone marrow trephine biopsy involvement by lymphoma: pattern of involvement and concordance with flow cytometry, in 10 years from a single institution.

PURPOSE: To evaluate the features of bone marrow (BM) biopsy involvement by lymphoma, pattern of infiltration, morphological analysis and flow cytometry were reviewed at all lymphoma patients over a period of 10 years. METHODS/PATIENTS: 413 cases were included in the study if BM biopsy slides were available. Only 356 patients had both BM trephine biopsy and flow cytometry. RESULTS: The most frequent subtype was diffuse large B cell (31.2%), followed by follicular lymphoma (18.9%). The predominant pattern was mixed (nodular-interstitial) (9.2%). Morphological marrow infiltration was found in 138 cases, and flow cytometry identified 117 cases with BM involvement. A concordance between the two methods was detected in 305 cases (85.7%). There was discordance in 51 cases (14.3%): morphology positive/FC negative in 33 cases and morphology negative/FC positive in 18. CONCLUSIONS: Flow cytometry is slightly more useful in detecting involvement when the BM is affected, but this finding is not conclusive.

Inter-observer variance with the diagnosis of myelodysplastic syndromes (MDS) following the 2008 WHO classification.

Morphology is the basis of the diagnosis of myelodysplastic syndromes (MDS). The WHO classification offers prognostic information and helps with the treatment decisions. However, morphological changes are subject to potential inter-observer variance. The aim of our study was to explore the reliability of the 2008 WHO classification of MDS, reviewing 100 samples previously diagnosed with MDS using the 2001 WHO criteria. Specimens were collected from 10 hospitals and were evaluated by 10 morphologists, working in five pairs. Each observer evaluated 20 samples, and each sample was analyzed independently by two morphologists. The second observer was blinded to the clinical and laboratory data, except for the peripheral blood (PB) counts. Nineteen cases were considered as unclassified MDS (MDS-U) by the 2001 WHO classification, but only three remained as MDS-U by the 2008 WHO proposal. Discordance was observed in 26 of the 95 samples considered suitable (27 %). Although there were a high number of observers taking part, the rate of discordance was quite similar among the five pairs. The inter-observer concordance was very good regarding refractory anemia with excess blasts type 1 (RAEB-1) (10 of 12 cases, 84 %), RAEB-2 (nine of 10 cases, 90 %), and also good regarding refractory cytopenia with multilineage dysplasia (37 of 50 cases, 74 %). However, the categories with unilineage dysplasia were not reproducible in most of the cases. The rate of concordance with refractory cytopenia with unilineage dysplasia was 40 % (two of five cases) and 25 % with RA with ring sideroblasts (two of eight). Our results show that the 2008 WHO classification gives a more accurate stratification of MDS but also illustrates the difficulty in diagnosing MDS with unilineage dysplasia.

Magnetic navigation and voltage mapping guided implantation of a pacemaker atrial lead in a previously unpaceable patient.

We report successful implantation of the atrial pacing lead in a patient in whom such operation had previously failed with the manual approach. Right atrial (RA) electro-anatomical voltage mapping was used to identify an area suitable for pacing and magnetic navigation to allow exhaustive RA exploration leading to successful RA lead screwing.

[Arrhythmia and sport]. / Arythmies du sportif.

Sports arrhythmia has gained wide attention with the mediatization of the death of famous sports stars. Sport strongly modifies the structure of the heart with the development of left ventricular hypertrophy which may be difficult to differentiate from that due to doping. Intense training modifies also the resting electrocardiogram with appearance of signs of left ventricular hypertrophy whereas resting sinus bradycardia and atrioventricular conduction disturbances usually reverts upon exertion. Accordingly, arrhythmia may develop ranging from extrasystoles to atrial fibrillation and even sudden death. Recent data suggest that if benign arrhythmia may be the result of the sole intense training and are reversible, malignant ventricular arrhythmia and sudden death mostly occur in unknown structural heart disease. Hypertrophic cardiomyopathy is amongst the most frequent post mortem diagnosis in this situation. Doping is now present in many sports and further threatens the athlete in the safe practice of sport.

[New concepts in the diagnosis and treatment of atrial flutter]. / Nouveaux concepts dans la prise en charge diagnostique et thérapeutique du flutter atrial.

Atrial flutter may now be very frequently and definitely cured in a single session of radiofrequency ablation. However, the very name of atrial flutter gives rise to a certain confusion. Clinical experience from everyday activity in ablation laboratories, especially since the introduction of new mapping techniques, has shown that this entity is in fact multiple. Flutters may be classified by their electrocardiographic appearance and/or their electrophysiological mechanism with as many prognostic as therapeutic implications. This article reviews diagnostic features of typical and atypical flutter and the different treatments which may be proposed in different clinical situations.

[Ventricular tachycardia "in salvoes"]. / Les tachycardies ventriculaires en salves.

The term of ventricular tachycardia "in salvoes" describes electrophysiographic appearances of several consecutive ectopic ventricular beats without interposition of sinus rhythm. This is an intermediate arrhythmic state between isolated ventricular extrasystoles and sustained ventricular tachycardia. The generally accepted definition of the term "sustained" implies a duration of over 30 seconds or poor haemodynamic tolerance. Strictly speaking, the term "salvoe" has no precise definition in cardiology. In the 1996 edition of the Petit Robert French dictionary, the term is defined as the simultaneous discharge of guns or successive blasts of canons. The Delaware medical dictionary does not provide a French definition of the term "salvoe". In practice, we use the term tachycardia in salvoes in the same meaning as ventricular tachycardia. Schematically, in clinical practice, two situations may be encountered. In the first case, salvoes of VT are recorded in apparently normal hearts; they are not life-threatening and, though often nearly asymptomatic, they may pose therapeutic problems. In the second case, the arrhythmia occurs in a diseased heart, with a low ejection fraction, in which the essential problem is the vital prognosis.

Atypical atrial flutters.

Typical atrial flutter is due to a counterclockwise macro-re-entry circuit localized in the right atrium with a surface ECG pattern showing predominantly negative F waves in the inferior leads and positive F waves in V1. Recently it has been proposed to classify atrial flutter on the basis of its cavo-tricuspid isthmus dependence rather than on the ECG pattern. Therefore some atrial flutters are considered typical even if the ECG does not exhibit a typical pattern. This is the case for reverse typical atrial flutter, lower loop re-entry and partial-isthmus-dependent short circuit flutter. The term atypical flutter refers to a non-isthmus dependent flutter. Usually these patients have had previous cardiac surgery with a right or left atriotomy. Flutter involving a spontaneous right atrial scar is not uncommon.

[Cardioversion by external electric shock for atrial fibrillation: does patient age affect immediate results?]. / Cardioversion par choc électrique externe de la fibrillation auriculaire: l'âge des patients affecte-t-il les résultats immédiats?

The restoration of sinus rhythm by external electric shock in patients with persistent atrial fibrillation is a well established treatment. However, in current practice this treatment is generally indicated less in the elderly subject although this attitude is not factual. The objective of this work was to evaluate the immediate results of cardioversion by external electric shock, comparing the success rates in four age groups: under 60 years, between 60 and 69 years, between 70 and 79 years, and over 80 years. This study was performed on 182 consecutive patients aged from 25 to 89 years: 35 patients aged less than 60 years, 52 patients aged from 60 to 69 years, 65 patients aged from 70 to 79 years, and 30 patients aged 80 years or over. The success rates were 91.4% before 60 years, 90.4% between 60 and 69 years, 90.8% between 70 and 79 years, and 83.3% after 80 years. There was no significant difference between the success rates in the four age groups (p = 0.68). Among the other factors analysed, only the duration of atrial fibrillation and the body mass index significantly influenced the results of external electric shock in this series. This work suggests that age does not significantly influence the immediate results of external electric shock. According to these data it does not appear justified to contra-indicate cardioversion by external electric shock on the sole criterion of age.

Catheter ablation using very high frequency current: effects on the atrioventricular junction and ventricular myocardium in sheep.

BACKGROUND: Radiofrequency ablation is currently used in the treatment of various cardiac arrhythmias. However, this technique is limited by impedance rise, leading to coagulum formation and desiccation of tissue. We developed a new generator, providing very high frequency (27 MHz) current, which is in the intermediate range between radiofrequency and microwave energy. The aim of this study was to evaluate the results for catheter ablation of the atrioventricular junction and characteristics of the lesions obtained at ventricular sites. METHODS AND RESULTS: The generator was coupled to a specially designed 7-French coaxial catheter. The study included experiments performed on 10 sheep (Wt. 31- 42 kg). In seven sheep, the catheter was introduced into the femoral vein and advanced across the tricuspid annulus to record the largest possible His electrogram. VHF current was applied for 25 s, with increasing energies. The energy needed to obtain complete atrioventricular (AV) block ranged from 60 to 100 Watts. Six animals were observed for 6 to 21 days. Complete AV block was found to be persistent. In those seven sheep in whom AV junction was ablated and in three additional sheep, the ablation catheter was positioned toward the right ventricular apex using the same approach and into the left ventricle via the femoral artery, and 20 to 90 Watts energy was delivered in order to assess the size of the induced lesions. Side effects included ventricular tachycardia degenerating into ventricular fibrillation in six cases, but the same effect was observed in this animal model with radiofrequency energy. No cardiac perforation was noted. No thrombus was observed at the catheter tip. The size of the lesion ranged from 3 to 45 mm in width and 1 to 15 mm in depth. CONCLUSIONS: Catheter ablation using VHF current is feasible and appears effective in producing stable AV block when applied at the AV junction and results in substantial myocardial lesions. Further studies are needed to define its clinical interest and side effects.

External cardioversion of atrial fibrillation: comparison of biphasic vs monophasic waveform shocks.

AIMS: It is well established in transthoracic ventricular defibrillation that biphasic truncated waveform shocks are associated with superior defibrillation efficacy when compared with damped sine wave monophasic waveform shocks. The aim of this study was to explore whether biphasic waveform shocks were superior to monophasic waveform shocks for external cardioversion of atrial fibrillation (AF). METHODS AND RESULTS: Fifty-seven patients in whom cardioversion of AF was indicated were randomized in this prospective study, to transthoracic cardioversion with either monophasic damped sine waveform shocks or biphasic impedance compensating waveform shocks. In the group randomized to monophasic waveform shocks (27 patients), a first shock of 150 J was delivered, followed (if necessary) by a 360 J shock. In the biphasic waveform group (30 patients), the first shock had an energy of 150 J and (if necessary) a second 150 J was delivered. All shocks were delivered in the anterolateral chest pad position. Sinus rhythm was restored in 16 patients (51%) with the first monophasic shock and in 27 patients (86%) with the first biphasic shock. The difference was statistically significant (P=0.02). After the second shock, sinus rhythm was obtained in a total of 24 patients (88%) with monophasic shocks and in 28 patients (93%) with biphasic shocks. No complication was observed in either group and cardiac enzymes (CK, CKmb, troponin I, myoglobin) did not show any significant changes. CONCLUSION: This study suggests that at the same energy level of 150 J, biphasic impedance compensating waveform shocks are superior to monophasic damped sine waveform shocks cardioversion of atrial fibrillation.

Tratamiento del deterioro agudo de la función neural en la lepra: resultado de un estudio prospectivo de cohort en Bangladesch

Se informa del resultado de 132 pacientes con deterioro agudo de la función nwerviosa (NFI) a los nervios sensitivos y un 67% de los motores evidenciaron mejoría , sin diferencia estadísticamente significativo en la respuesta de varios nervios a la prednisolona. La duración y gravedad del deterioro no son indicadores significativos del resultado terapéutico. Un total de 32% de los nervios determinados no respondieron el tratamiento con prednisolona y un 12% de los nervios afectados continuaron con deterioro funcional a pesar del tratamiento. En el grupo tratado, el valor medio ojo-mano-pie (EHF) se incrementó desde 2.02 al 1.33 (el valor mediano pasó de 2 a 1). Aproximadamente un tercio de los pacientes no recibieron tratamiento con prednisolona, motivo suficiente para que algunos presentaron NFI nuevo frente a un historial de deterioro crónico y resultaron NFI omitidos. El grupo de pacientes "injustamente tratados" presentó una mejoría espontánea de la función nerviosa sensorial del NFI del 62% de la función motora a los 12 meses desde el inicio de NFI. El valor EHF no registró ninguma mejoría estadísticamente significativa.

Inhibition of Na,K-ATPase by external electrical cardioversion in a sheep model of atrial fibrillation.

INTRODUCTION: Electrical external cardioversion commonly used to treat atrial fibrillation (AF) is associated with myocardial membrane damage and disturbances in ionic homeostasis (hemodynamically unstable). The present study was designed to investigate whether alterations in ionic homeostasis observed were due in part to changes in the myocardial activity of Na,K-ATPase. METHODS AND RESULTS: AF was induced by pacing in ten anesthetized sheep divided into two groups. Group I (n = 4) received a single external countershock of 360 J after three episodes of AF lasting 10 minutes. Group II (n = 6) served as controls. Activity, responsiveness to ouabain, and membrane expression of catalytic alpha and beta subunits of Na,K-ATPase in sarcolemmal myocardial membrane fractions were investigated. Membrane fluidity and fatty acid composition, and plasma levels of atrial natriuretic factor (ANF) also were measured. One shock after episodes of AF significantly decreased ventricular Na,K-ATPase activity up to 50% (P < 0.001) without modification of atrial activity at the membrane level. Sites with low affinity to ouabain showed a fivefold lower affinity for ouabain in the cardioversion group than in the control group (IC50 = 7.9 micromol/L vs 40 micromol/L ouabain, P < 0.05). Plasma levels of ANF were significantly increased in the cardioversion group compared with the control group. These changes were independent of membrane modulation in terms of expression of Na,K-ATPase, membrane fluidity, and fatty acid composition. CONCLUSION: This study suggests that left ventricular perturbation of ionic homeostasis subsequent to transthoracic cardioversion could result from inactivation of Na,K-ATPase activity.

Interelectrode (Accufix) lead fracture.

An interelectrode fracture was diagnosed 7 years after the implantation of an Accufix lead. The lead body separated from the tip with the helix screwed into the atrial wall. The retention wire was intact and may have contributed to the lead rupture.

[Hereditary resistance to vitamin K antagonists. Apropos of a case]. / Résistance héréditaire aux antivitamines K. A propos d'un cas.

Resistance to vitamin K antagonists is a rare phenomenon. In general, it is the result of poor patient compliance, malabsorption, a diet rich in vitamin K, or the use of enzyme inducers. Occasionally, the diagnosis of resistance is made when plasma levels are high. The authors report a case of hereditary resistance to vitamin K antagonists in a 43 year old man admitted for atrial fibrillation. The precise prevalence of this anomaly is unknown. However, the existence should be known to clinicians who often use this important family of drugs.

A role for the renin-angiotensin system in the evolution of cardiac memory.

INTRODUCTION: We studied the role of the cardiac renin-angiotensin II system in the genesis of cardiac memory, in which T wave changes induced by ventricular pacing (VP) accumulate and persist during subsequent sinus rhythm. METHODS AND RESULTS: Anesthetized dogs were instrumented via a thoracotomy and three 20-minute runs of VP were interspersed with periods of normal sinus rhythm sufficient to permit T wave recovery to 90% of control. Memory was quantified as the change (delta) in T wave vector angle showing accumulation over the three monitoring periods. In five control dogs T wave vector = -27 +/- 49 degrees, and this shifted by 104 degrees (P < 0.05) over the three postpacing recovery periods. In seven dogs infused with the receptor blocker saralasin, five infused with the angiotensin-converting enzyme inhibitor captopril, and four infused with the tissue protease inhibitor chymostatin, there were significant reductions in the incidence and the accumulation of memory. In four other experiments, we used isolated, blood-perfused canine hearts to demonstrate that VP used to induce memory alters the contractile pattern of the left ventricle. CONCLUSIONS: We propose that the alteration in myocardial stretch induced by pacing activates angiotensin II synthesis by cardiac cells. We propose, further that the endogenous cardiac renin-angiotensin II system (blocked by saralasin, captopril and by chymostatin) is an important contributor to the induction of memory.

[Heart perforation following transvenous implantation of a cardiac pacemaker]. / Perforation due coeur après implantation transveineuse de stimulation cardiaque.

OBJECTIVES: We studied the incidence, clinical signs and severity of heart perforations occurring after transvenous pacemaker implantation. PATIENTS AND METHODS: A series of 16 consecutive cases of heart perforation observed in one cardiac pacing unit from 1989 to 1998 were reviewed. RESULTS: Heart perforation occurred after implantation in 9 cases; the verall incidence for all lead implantation was 0.57%. The ventricle was perforated in 6 cases, the atrium in 1, and an undetermined site in 2 cases. Active fixation was involved in 5 cases, passive fixation in 4. A bipolar lead was used in 7 cases and a unipolar lead in 2. Heart perforation occurred after prior external stimulation in 7 cases, including one case with tamponnade requiring emergency pericardial drainage after implantation. Repositioning the lead in the ventricle was sufficient in 6 cases and a thoracotomy for an atrial wound was performed in 1 case. Difficulties in right ventricular catheterism due to kyphoscoliosis in elderly subjects was found to be a risk factor. CONCLUSION: Heart perforation following transvenous pacemaker implantation is an exceptional complication with currently used material. Tamponnade is extremely rare. Besides verifying the mechanical performance of the leads, prevention requires a rigorous protocol for catheterism and wall fixation.

Internal low-energy cardioversion: a therapeutic option for restoring sinus rhythm in chronic atrial fibrillation after failure of external cardioversion.

AIMS: Conventional external cardioversion remains the technique of choice for restoration of sinus rhythm in patients with chronic atrial fibrillation (AF). Recent reports have suggested that internal low-energy cardioversion is efficient and safe in terminating AF in patients with failed external cardioversion. METHODS AND RESULTS: In 20 of 118 consecutive patients with spontaneous chronic AF (>7/days), who underwent low-energy cardioversion, one or more attempts at restoring sinus rhythm with external cardioversion had failed. Low-energy internal cardioversion was performed under light sedation. Shocks were delivered (using an external custom defibrillator) between two nonapolar catheters positioned in the right atrium (cathode) and in the coronary sinus (anode). Heart disease was present in 12 and absent in eight patients ('lone' atrial fibrillation). Atrial fibrillation was established for a period ranging from 12 days to 53 months. Low-energy internal cardioversion restored sinus rhythm in 15 of the 20 patients (75%) with a mean energy of 4.5+/-1.2 J, a mean conversion voltage of 355+/-53 V and a mean impedance of 63+/-8 ohms. No complications were observed. With a mean follow-up of 6+/-7 months, 11 patients (73%) were in stable sinus rhythm. CONCLUSIONS: This study provides evidence in support of low-energy internal cardioversion as a valuable therapeutic option in patients in whom conventional external cardioversion failed. This technique is safe and does not require general anaesthesia.

[Implantable ventricular defibrillator. Systematic induction of ventricular arrhythmia is not useful except for implantation]. / Défibrillateur ventriculaire implantable. L'induction systématique d'une arythmie ventriculaire n'est pas utile en dehors de l'implantation.

Checking the efficacy of a defibrillator after its implantation is current practice. This control usually entails induction of ventricular fibrillation (VF). The aim of this study was to assess the utility of this practice in patients with an endovascular system of electrodes. Implantation was acquired when a margin of security of 10 days or more had been obtained. During the postoperative test, the choice of energy was that which reduced VF at implantation. Of the 84 patients included in this study, 59 were implanted with an endovascular electrode system alone and the other 25 patients had endovascular electrodes associated with a subcutaneous patch electrode. The average time from implantation to the postoperative test was 31 +/- 12 days. Arrhythmia sensing was normal and reduction by the defibrillator was observed in all patients. The average energy of the electric shocks was not significantly different to that observed at implantation (18.6 +/- 3.6 J and 19.3 +/- 4.7 J). Fifty patients had antiarrhythmic drugs (amiodarone = 43) at implantation, and 32 at the time of the postoperative test (amiodarone = 17). This study showed that the postoperative test with induction of VF was normal in all patients. Therefore, the authors propose that follow-up should consist of consultation including interrogation of the defibrillator and a chest X-ray to check the position of the electrodes. If one of these tests is abnormal or if the perioperative threshold of defibrillation does not provide an adequate margin of safety, induction of a ventricular arrhythmia is necessary to check the function of the system.

[Utilization of transcutaneous anesthetics during heart catheterization]. / Utilisation des anesthésiques transcutanés au cours des cathétérismes cardiaques.

OBJECTIVES: Assess the efficacy of an anesthetic cream for cardiac catheterization. PATIENTS AND METHODS: Percutaneous anesthesia was studied in a series of 100 consecutive patients undergoing cardiac catheterization. The anesthesia was composed with an eutetic mixture of local anesthetics and applied precisely over the puncture area in a randomized controlled study. After admission, patients were randomized into two groups: 50 patients received lidocaine infiltration and 50 patients received associated cream and infiltration. Percutaneous anesthesia was to be applied 2 hours before entering the operating room. RESULTS: No complication developed with this cream combined with lidocaine infiltration. Serum concentration indicated very low levels which were very well tolerated. Patient comfort improved with the anesthetic cream-lidocaine infiltration association. CONCLUSION: The use of an anesthetic cream is safe and effective, especially combined with lidocaine infiltration during cardiac catheterization. Cost is high and the association might be reserved for special indications (obesity, children).