ABSTRACT
Background: The use of novel materials as an artificial extracellular matrix for stem cell growth is a current strategy of increasing interest for regenerative medicine. Here, we prepare thermal-remolded membrane scaffolds from poly(3-hydroxybutyrate) grafted with 2-amino-ethyl methacrylate hydrochloride. However, it is unclear whether these membranes are useful for tissue engineering. Results: The mechanical properties, tribology, and morphology of the dense membranes were assessed. The results show that tensile strain at break and roughness of the compressed membrane decrease with increasing graft degree. Moreover, graft copolymer membranes showed lower resistance to scratching, greater degree of swelling and higher brittleness than un-grafted P(3HB) films. Thus, it effectively supports the growth of dermal fibroblast, as demonstrated by epifluorescence microscopy. Conclusions: It is concluded that the developed membrane can be properly used in is the restoration of skin tissue. How to cite: González-Torres M, Sánchez-Sánchez R, Solís-Rosales SG, et al. Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth.
Subject(s)
Mesenchymal Stem Cells/physiology , Membranes, Artificial , Temperature , Regenerative Medicine , GrowthABSTRACT
A total of 39 outbreaks of foodborne diseases affecting 958 people in the province of Rio Negro, Argentina between 1993 and 2001 are described and evaluated. The main causal agents were identified involving food, sites of occurrence, risk factors and notification system used. Salmonella spp (38%), Trichinella spiralis (15%), Escherichia coli (13%) and Staphylococcus aureus (15%) were the most frequent agents present in outbreaks. Salmonella spp produced the largest number of cases (52%). Food involved were cooked meat (36%), cheese (10%), sandwiches (10%), deserts (10%) and ice cream (8%). Indeed, ice creams were involved in the largest number of cases and of people affected. In relation to the source of food, 41% of outbreaks were caused by homemade meals, 23% by catering or ice cream parlor, 13% in family parties, 8% in county fairs and 8% in hotel restaurants. In 28% of the outbreaks the etiological agent was identified exclusively by epidemiological analysis, in 64% isolation of the agent was carried out, and in 8% of the cases, a final diagnosis could not be obtained. Validity of epidemiological studies in foodborne disease, the necessity of strengthening the notification system of outbreaks, and the importance of good practices in food handling are analyzed.
Subject(s)
Disease Outbreaks , Food Microbiology , Foodborne Diseases/epidemiology , Gram-Negative Bacteria/isolation & purification , Population Surveillance , Argentina/epidemiology , Epidemiologic Studies , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Food Handling , Foodborne Diseases/microbiology , Humans , Salmonella Food Poisoning/epidemiology , Salmonella Food Poisoning/microbiology , Staphylococcal Food Poisoning/microbiology , Trichinellosis/epidemiology , Trichinellosis/microbiologyABSTRACT
A total of 39 outbreaks of foodborne diseases affecting 958 people in the province of Rio Negro, Argentina between 1993 and 2001 are described and evaluated. The main causal agents were identified involving food, sites of occurrence, risk factors and notification system used. Salmonella spp (38
), Trichinella spiralis (15
), Escherichia coli (13
) and Staphylococcus aureus (15
) were the most frequent agents present in outbreaks. Salmonella spp produced the largest number of cases (52
). Food involved were cooked meat (36
), cheese (10
), sandwiches (10
), deserts (10
) and ice cream (8
). Indeed, ice creams were involved in the largest number of cases and of people affected. In relation to the source of food, 41
of outbreaks were caused by homemade meals, 23
by catering or ice cream parlor, 13
in family parties, 8
in county fairs and 8
in hotel restaurants. In 28
of the outbreaks the etiological agent was identified exclusively by epidemiological analysis, in 64
isolation of the agent was carried out, and in 8
of the cases, a final diagnosis could not be obtained. Validity of epidemiological studies in foodborne disease, the necessity of strengthening the notification system of outbreaks, and the importance of good practices in food handling are analyzed.
ABSTRACT
Se realizó un estudio para conocer la sensibilidad y especificidad de una nueva prueba in vitro (Phadiatrop Pediátric) útil en el diagnóstico de alergia en la edad pediátrica en 65 niños divididos en dos grupos. El grupo 1 (35 pacientes) con diagnóstico de alergia respiratoria (asma, rinitis alérgica o ambas), basaso en la historia clínica, eosinófilos en el moco nasal, determinación de IgE y preuebas cutáneas, y el grupo 2 (30 pacientes) sin diagnóstico de alergia. En ambos grupos se cuantificó la IgE y se realizó la prueba de Phadiatop paediatric. En el grupo I la prueba fue positiva en 28 pacientes (80 por ciento y en el grupo 2 lo fue a dos pacientes (6.6 por ciento) que sifnifica una sensibilidad de 67.5 por ciento, con una especificidad de 88.0 por ciento para esta prueba