1.
Chinese Journal of Medical Genetics
; (6): 622-625, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-254548
ABSTRACT
<p><b>OBJECTIVE</b>To analyze allele dropout at TH01 locus in paternity testing in order to determine the accurate genotype.</p><p><b>METHODS</b>To use a two STR loci genotyping system to verify an abnormal genotype for the TH01 locus with PCR using specific primers, cloning and DNA sequencing.</p><p><b>RESULTS</b>A rare allele at TH01 locus named 5.2, which was undetectable with PowerPlex 21 system, was detected with an Identifiler system.</p><p><b>CONCLUSION</b>Genetic variations may result in rare alleles and loci loss. To avoid misjudgment, laboratories should have a variety of methods for detecting loci loss.</p>