ABSTRACT
Objective: To investigate the effects of connective tissue growth factor (CTGF) on proliferation and migration of human osteosarcoma 143B cells in simulated osteosarcoma microenvironment by coculture technique in vitro, and to explore their underlying mechanisms. Methods: The osteosarcoma 143B cells were infected with recombinant adenovirus AdCTGF, then an indirect co-culture system was established with bone marrow stromal HS-5 cells for 3 days. The proliferation capacity of 143B cells was detected by MTT, and the migration ability of 143B cells was determined by wound-healing test and Transwell assay. The expression levels of CTGF and β-catenin were examined by reverse transcription-PCR (RT-PCR) and Western blotting. The expression levels of phosphorylated Akt (p-Akt) and phosphorylated glycogen synthase kinase 3β (p-GSK3β) were examined by Western blotting. Results: After infection with recombinant adenovirus AdCTGF, CTGF was overexpressed in osteosarcoma 143B cells in co-culture system. In simulated osteosarcoma microenvironment in vitro, CTGF overexpression could significantly promote the proliferation of 143B cells (P < 0.01), and the wound-healing rate and the number of migratory 143B cells also remarkably increased (both P < 0.01). The expression levels of P-catenin, p-Akt and p-GSK3β increased significantly in 143B cells after overexpressing CTGF (all P < 0.05). Conclusion: CTGF can significantly promote the proliferation and migration of human osteosarcoma 143B cells in simulated osteosarcoma microenvironment. PI3K (phosphatidylinositol 3-kinase)/Akt and Wnt/β-catenin pathways may be involved in this process.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between lifestyle factors, parental obesity and adiposity in children, in order to provide theoretical evidence for public health policy establishment.</p><p><b>METHODS</b>A cross-sectional observation study was conducted among approximately 20 thousand children aged 2 - 18 years old in urban and rural regions of Beijing, by using stratified randomization clustering sampling methods. Familial environmental risk factors of children adiposity and parental obesity were assessed with standardized questionnaire. Anthropometric measurements, including height and weight, were conducted. SPSS 13.0 software was applied to analyze the data, including general description, chi(2) trend test and non-condition logistic analyse.</p><p><b>RESULTS</b>With IOTF obesity references, the prevalence of obesity in 21,198 children aged 2 - 18 years old was 5.6%. The behavioral characters (for example, smoking and drinking) and children obesity showed significant familial aggregation. In groups including "both parents not smoke", "only one parent smoke" and "both parents smoke", the smoking rates of offsprings were 1.50%, 2.93% and 6.01%, respectively (chi(trend)(2) = 107.009, P < 0.01). A similar pattern was found for offsprings' alcohol consumption rates (5.85%, 9.12% and 13.96%; chi(trend)(2) = 107.009, P < 0.01). Based on parents' BMI status, in groups including "both parents had normal weight", "father was obese", "mother was obese" and "both parents were obese", the prevalence of obesity in children were 3.29%, 11.48%, 9.12% and 27.01%, respectively (chi(trend)(2) = 293.404, P < 0.01). After controlling for sex and ages, factors such as physical exercises, sleeping times per day, fat intakes, watching TV, drinking alcohol were impact factors of children obesity. After controlling of confounding factors, such as children gender, age, birth weight, puberty, smoking history, drinking history, fat intakes, soft drink, physical exercises, education experiences of parents, smoking history, drinking history, family income and so on, maternal obesity had a greater influence on daughters than on sons (OR = 5.93, 95% CI: 3.57 - 9.84), and paternal obesity showed similar influence on sons (OR = 4.29, 95% CI: 3.21 - 5.72). Comparing to parents with normal weight, obese parents had more powerful impact on daughters (OR = 28.51, 95% CI: 15.13 - 53.72) than on sons (OR = 7.21, 95% CI: 4.07 - 12.75), regarding to 2 - 5 years group and 10 - 12 years group versus other age group (OR = 18.67, 95% CI: 1.49 - 234.46; OR = 22.25, 95% CI: 10.62 - 46.59).</p><p><b>CONCLUSION</b>Parental obesity is an independent risk factor of adiposity in children; gender and age affect this association. The lifestyle patterns of parents should have great impact on children. When prevention or intervention with children obesity, familial environmental factors should be emphasized.</p>
