Study on Compatibility Stability of Ulinastatin for Injection with 2 Commonly Used Solvents in the Infusion Pump / 中国药房

OBJECTIVE:To investigate the compatibility stability of Ulinastatin for injection with 2 commonly used solvents in the infusion pump.METHODS:After Ulinastatin for injection 500 000 U was respectively added into 0.9％ Sodium chloride injection and 5％ Glucose injection 50 mL,the appearance of the mixture at 25 ℃,37 ℃ were observed at 0,1,2,4,8,12,24 h,re spctively,pH value and the number of insoluble particles were measured.The relative percentage of ulinastatin in the mixture was determined by HPGFC.RESULTS:Under this condition,the appearance and pH value of the mixture had no significant change within 24 h;the number of particles ≥10 μm was lower than 25 particle/mL,and that ≥25 μm was lower than 3 particle/mL,which was in line with the pharmacopeia standard.The relative percentage of ulinastatin within 24 h ranged 99.45％-102.55％.CONCLUSIONS:After mixed with 0.9％ Sodium chloride injection and 5％ Glucose injection,Ulinastatin for injection keep stable within 24 h at 25 ℃,37 ℃ and can be used for continuous administration in the infusion pump.

Kallistatin, a new and reliable biomarker for the diagnosis of liver cirrhosis

Kallistatin, which protects organs and cells against inflammation, fibrosis and oxidative stress, is mainly synthesized and secreted in liver. However, its relationship to human liver disease remains unclear. The purpose of this study was to explore the relationship between serum kallistatin and clinical evidence of both cirrhosis and hepatocellular carcinoma (HCC), and to determine if serum kallistatin levels could be used as a diagnostic indicator of hepatic health status, especially human liver cirrhosis (LC). Our cohort consisted of 115 patients with clinically proven liver fibrosis (LF), LC, or HCC by liver biopsies, and 31 healthy controls (CON). Serum kallistatin levels were quantified by ELISA. Results of the present study demonstrated that irrespective of the underlying etiology, serum kallistatin levels were significantly lower in the LF/LC group when compared with the CON group. A decrease in serum kallistatin levels appeared to reflect the extent of cirrhosis, with the lowest levels associated with higher grades of cirrhosis. Patients with LC had a noticeable correlation between serum kallistatin levels and other serum biochemical indicators. The area under the curve (AUC) for LC, viral liver cirrhosis (VLC) and alcoholic liver cirrhosis (ALC) was 0.845, 0.757 and 0.931, respectively. In conclusion, our findings demonstrated that kallistatin, a plasma protein produced by the liver, can be a useful and reliable diagnostic indicator of hepatic health status, especially for LC.

Radial vibration and ultrasonic field of a long tubular ultrasonic radiator.

The radial vibration of a metal long circular tube is studied analytically and its electro-mechanical equivalent circuit is obtained. Based on the equivalent circuit, the radial resonance frequency equation is derived. The theoretical relationship between the radial resonance frequency and the geometrical dimensions is studied. Finite element method is used to simulate the radial vibration and the radiated ultrasonic field and the results are compared with those from the analytical method. It is concluded that the radial resonance frequency for a solid metal rod is larger than that for a metal tube with the same outer radius. The radial resonance frequencies from the analytical method are in good agreement with those from the numerical method. Based on the acoustic field analysis, it is concluded that the long metal tube with small wall thickness is superior to that with large wall thickness in producing radial vibration and ultrasonic radiation. Therefore, it is expected to be used as an effective radial ultrasonic radiator in ultrasonic sewage treatment, ultrasonic antiscale and descaling and other ultrasonic liquid handling applications.

Radially composite piezoelectric ceramic tubular transducer in radial vibration.

The radially composite piezoelectric tubular transducer is studied. It is composed of radially poled piezoelectric and a long metal tube. The electro-mechanical equivalent circuit of the radially poled piezoelectric and metal tube in radial vibration is obtained. Based on the force and velocity boundary conditions, the six-port electro-mechanical equivalent circuit for the composite tubular transducer is given and the resonance/anti-resonance frequency equations are obtained. The relationship between the resonance frequency and the dimensions is analyzed. Numerically simulated results obtained by the finite element method are compared with those from the analytical method. Composite piezoelectric tubular transducers are designed and manufactured. The resonance/anti-resonance frequencies are measured, and it is shown that the theoretical results are in good agreement with the simulated and experimental results. It is expected that radially composite piezoelectric tubular transducers can be used as high-power ultrasonic radiators in ultrasonic applications, such as ultrasonic liquid processing.

Analysis of scatterer microstructure feature based on Chirp-Z transform cepstrum / 生物医学工程学杂志

The fundamental research field of medical ultrasound has been the characterization of tissue scatterers. The signal processing method is widely used in this research field. A new method of Chirp-Z Transform Cepstrum for mean spacing estimation of tissue scatterers using ultrasonic scattered signals has been developed. By using this method together with conventional AR cepstrum method, we processed the backscattered signals of mimic tissue and pig liver in vitro. The results illustrated that the Chirp-Z Transform Cepstrum method is effective for signal analysis of ultrasonic scattering and characterization of tissue scatterers, and it can improve the resolution for mean spacing estimation of tissue scatterers.

Load characteristics of high power sandwich piezoelectric ultrasonic transducers.

Based on the equivalent circuit theory, the load characteristics of high power piezoelectric ultrasonic sandwich transducers are studied. Two types of loads are studied. One is liquid load as in ultrasonic cleaning, and the other is solid load as in ultrasonic drilling and machining. The effect of load and structure of the transducer on the resonance frequency of the transducer is analyzed. It is shown that the effect of load on the resonance frequency of sandwich transducers with different structures is different. For liquid load as in ultrasonic cleaning, the effect of the load on the resonance frequency of the sandwich transducer with symmetrical structure is the largest. It is the smallest for the transducer with its displacement node in the back metal cylinder. For solid load as in ultrasonic drilling and machining, the effect of the load on the resonance frequency of the sandwich transducer with its displacement node in the front metal cylinder is the largest. It is also the smallest for the transducer with its displacement node in the back metal cylinder. On the other hand, for some applications, such as ultrasonic drilling, when the lateral dimension of the tool is much less than that of the transducer, its effect on the resonance frequency of the transducer is small. The conclusions are useful in designing vibrating systems for different ultrasonic applications.

Analysis of the sandwich piezoelectric ultrasonic transducer in coupled vibration.

The coupled vibration of the sandwich piezoelectric transducer with a large cross-section is analyzed using an approximate analytic method. The resonance frequency equations of the transducer are derived and the effect of the geometrical dimensions on the resonance frequency is studied. It is illustrated that when the radial vibration in the transducer is considered, the vibration of the sandwich transducer becomes more complex. Apart from the longitudinal resonance frequency, the radial resonance frequency can also be obtained. For comparison, numerical methods are also used to simulate the coupled vibration; the resonance frequency and the vibrational displacement distribution are computed. Compared with one-dimensional longitudinal theory, the radial dimensions of the transducer are no longer limited because the coupled vibration is considered. Compared with numerical methods, the physical meaning of the analytic method is concise. It is illustrated that the resonance frequencies obtained from the coupled resonance frequency equations are in good agreement with those from numerical methods, and they are in better agreement with the measured results than those from one-dimensional theory. Since the radial and the coupled vibration are considered in the analysis, more resonance frequencies can be obtained. Therefore, using the coupled resonance frequency equations, the sandwich transducer with multifrequency or wide frequency bandwidth can be designed and used in ultrasonic cleaning, ultrasonic sonochemistry and other applications.

Optimization of the performance of the sandwich piezoelectric ultrasonic transducer.

The resonance and antiresonance frequency, the effective electromechanical coupling coefficient, and the mechanical quality factor of a sandwich piezoelectric ultrasonic transducer are studied and optimized. The effect of the thickness of thick piezoelectric element electrodes on the transducer performance is analyzed. The effect of the length and position of the piezoelectric elements in the transducer is also studied. It is shown that, although using thick electrodes is beneficial for releasing heat produced by the piezoelectric elements, the effective electromechanical coupling coefficient and the mechanical quality factor are reduced. The length and the position of the piezoelectric elements affect the performances of the transducer. Increasing the length of the piezoelectric elements decreases the mechanical quality factor, but the effective electromechanical coupling coefficient increases. When the length reaches a certain value, the effective electromechanical coupling coefficient reaches a maximum value. When the piezoelectric elements are located at the geometrical center or the displacement node, the effective electromechanical coupling coefficient and the mechanical quality factor are maximized.

Study on the prestressed sandwich piezoelectric ceramic ultrasonic transducer of torsional-flexural composite vibrational mode.

Based on the classical torsional and flexural vibrational theory of a slender rod, the prestressed sandwich torsional-flexural composite mode piezoelectric ceramic ultrasonic transducer is studied. This type of transducer consists of the slender metal rods and the longitudinally and tangentially polarized piezoelectric ceramic rings. The resonance frequency equations for the torsional and flexural vibrations in the transducers are derived. The simultaneous resonance of the torsional and flexural vibrations in the transducer is acquired by correcting the length of the metal slender rods resulting from the piezoelectric ceramic elements. The experimental results show that the measured resonance frequencies of the transducers are in good agreement with the computed ones, and the measured resonance frequencies of the torsional and the flexural vibrations in the composite transducers are also in good agreement with each other.