Avaliação de diferentes técnicas de higienização das mãos para manipuladores de um biotério de criação e experimentação de camundongos / Evaluation of different hand hygiene techniques for handlers a laboratory animals husbandry

O presente estudo teve como objetivo quantificar, identificar e avaliar a carga microbiana veiculada pelas mãos de nossos manipuladores, bem como a correta utilização de equipamentos de proteção individual (EPIs). Foram analisadas 188 amostras colhidas das mãos dos manipuladores durante o processo de troca dos microambientes dos camundongos nos tempos 0, 30, 60, 90 e 120 minutos. Foram testados três diferentes métodos de lavagem e assepsia das mãos. Nossos dados indicam que a prática da lavagem das mãos com sabão bactericida e posterior desinfecção com álcool gel a 70% previne a disseminação de agentes promotores de surtos infecciosos em áreas de biotério. Outro fator relevante avaliado foi o tempo de utilização das luvas, não sendo recomendado o uso de um mesmo par por mais de 30 minutos.(AU)

This study aimed to quantify, identify and assess the microbial load conveyed by the hands of our handlers as well as the correct use of personal protective equipments (PPE). We analyzed 188 samples collected from the hands of handlers during the exchange of the mice microenvironments at 0, 30, 60, 90 and 120 minutes. We tested three different methods of washing and disinfecting the hands. Our results showed that the practice of hand washing with antibacterial soap, then alcohol 70% with subsequent disinfection with alcohol gel prevents the spread of infectious outbreaks in our facility. Another relevant factor evaluated was glove time using. We found that wearing the same pair of gloves for over 30 minutes is not recommended.(AU)

Avaliação de diferentes técnicas de higienização das mãos para manipuladores de um biotério de criação e experimentação de camundongos / Evaluation of different hand hygiene techniques for handlers a laboratory animals husbandry

O presente estudo teve como objetivo quantificar, identificar e avaliar a carga microbiana veiculada pelas mãos de nossos manipuladores, bem como a correta utilização de equipamentos de proteção individual (EPIs). Foram analisadas 188 amostras colhidas das mãos dos manipuladores durante o processo de troca dos microambientes dos camundongos nos tempos 0, 30, 60, 90 e 120 minutos. Foram testados três diferentes métodos de lavagem e assepsia das mãos. Nossos dados indicam que a prática da lavagem das mãos com sabão bactericida e posterior desinfecção com álcool gel a 70% previne a disseminação de agentes promotores de surtos infecciosos em áreas de biotério. Outro fator relevante avaliado foi o tempo de utilização das luvas, não sendo recomendado o uso de um mesmo par por mais de 30 minutos.

This study aimed to quantify, identify and assess the microbial load conveyed by the hands of our handlers as well as the correct use of personal protective equipments (PPE). We analyzed 188 samples collected from the hands of handlers during the exchange of the mice microenvironments at 0, 30, 60, 90 and 120 minutes. We tested three different methods of washing and disinfecting the hands. Our results showed that the practice of hand washing with antibacterial soap, then alcohol 70% with subsequent disinfection with alcohol gel prevents the spread of infectious outbreaks in our facility. Another relevant factor evaluated was glove time using. We found that wearing the same pair of gloves for over 30 minutes is not recommended.

Characterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus

We identified a gametocyte-specific protein of Plasmodium falciparum called Pf11-1 and provide experimental evidence that this molecule is involved in the emergence of gametes of the infected erythrocyte (gametogenesis). A mutant parasite clone, which has deleted over 90% of the PF11-1 gene locus, was an important control to establish the gametocyte-specific expression of the Pf11-1. Molecular analysis of the Pf11-1 deletion indicates that it is presumably due a chromosome breakage with subsequent "healing" by the addition of telomeric heptanucleotides. Moreover, similar DNA rearrangements are observed in most of the laboratory isolates during asexual propagation in vitro

Mechanisms of immune protection in the asexual blood stage infection by Plasmodium falciparum: analysis by in vitro and ex-vivo assays

Mechanisms of immune protection against the asexual blood stage infection by Plasmodium falciparum are reviewed. Recent studies of two independent lines of research developed at the Institute Pasteur, in humans and primate infections clearly indicate an obligatory interaction of antibodies and effector cells to express the anti-parasitic effect