ABSTRACT
The purpose of this paper was to determine the effect of anodization on the in vitro proliferation and adhesion of immortalized human keratinocytes (HaCats) and mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) in Titanium Grade 23 (Ti6Al4V ELI) discs and to describe the surface topography, roughness, and composition of dental implants (body and collar) and abutments submitted to an area-specific anodization process. HaCat cells and BM-MSCs were seeded onto discs with three different surface treatments: machined, area-specific anodization for abutments, and area-specific anodization for implant collars. Cell proliferation was assessed using a resazurin-based fluorescent dye on days 1, 3, and 7, while cell adhesion was examined using scanning electron microscopy (SEM). Surface topography, roughness, and composition were evaluated for six implant bodies with an anodized rough surface, six anodized implant smooth collars, and six anodized prosthetic abutments. Both HaCats and BM-MSCs showed increased viability over time (p < 0.001) with no statistically significant differences among the different surfaces (p = 0.447 HaCats and p = 0.631 BM-MSCs). SEM analysis revealed an enhanced presence and adhesion of HaCat cells on the anodized surface for the implant collars and an increased adhesion of BM-MSCs on both the anodized and machined surface abutments. The topography characteristics of the treated implants and abutments varied depending on the specific implant region. Chemical analysis confirmed the presence of oxygen, calcium, phosphorus, and sodium on the anodized surfaces. The area-specific anodization process can be utilized to create variable topography, increase the specific surface area, and introduce oxygen, calcium, phosphorus, and sodium to dental implants and abutments. While BM-MSCs and HaCat cells showed similar adhesion and proliferation on anodized and machined surfaces, a positive interaction between anodized Ti6Al4V ELI surfaces and these two cell lines present in the peri-implant mucosa was observed. Due to the limitations of the present study, further research is necessary to confirm these findings.
ABSTRACT
Los linfomas no Hodgkin conforman un grupo de neoplasias malignas de origen lin-foide, clasificadas, de acuerdo con su correspondiente estirpe celular, como linfomas de células B o T. En su mayoría, estas entidades, provienen de linfocitos B, siendo el más frecuente el linfoma difuso de células B grandes (DLBCL). En cavidad oral, los linfomas no-Hodgkin tienen una baja incidencia y su pronóstico, evolución clínica y respuesta a los tratamientos, dependen del subtipo presentado. Estas neoplasias pueden desarrollarse en los tejidos blandos de la cavidad oral o de forma central en los maxilares. Se reporta un paciente de 80 años con una lesión correspondiente a linfoma no Hodgkin de células B grandes en cavidad oral, que se trató con quimioterapia y radioterapia, respondiendo favorablemente al tratamiento. Asimismo, con el objetivo de describir este tipo de lesio-nes poco frecuentes en cavidad oral y de valorar el pronóstico que estas tienen, se realizó una revisión de la literatura. Se concluye que debido a que el pronóstico de este tipo de lesiones depende del subtipo de linfoma no Hodgkin del que se trate y de su localización anatómica resulta muy importante realizar un correcto diagnóstico, de modo que se logre aplicar el tratamiento más adecuado para cada caso.
Non-Hodgkin lymphomas make up a group of malignant neoplasms of lymphoid origin, according to their corresponding cell line, as B or T cell lymphomas classified. Mostly, these entities come from B lymphocytes, being the most frequent diffuse large B-cell lymphoma (DLBCL). In the oral cavity, non-Hodgkin lymphomas have low incidence and their prognosis, clinical evolution and response to treatments, depend on the subtype presented. These malignancies can develop in soft tissues of the oral cavity or centrally in the jaws. We report an 80-year-old patient with an injury corresponding to large B-cell non-Hodgkin lymphoma in the oral cavity, who has been treated with chemotherapy and radiotherapy, responding favorably to treatment. Likewise, in order to describe this infre-quent type of lesions of the oral cavity and to assess the prognosis they have, a literature review was performed. It is concluded that because of the prognosis of this type of lesions Non-Hodgkin lymphomas make up a group of malignant neoplasms of lymphoid origin, according to their corresponding cell line, as B or T cell lymphomas classified. Mostly, these entities come from B lymphocytes, being the most frequent diffuse large B-cell lymphoma (DLBCL). In the oral cavity, non-Hodgkin lymphomas have low incidence and their prognosis, clinical evolution and response to treatments, depend on the subtype presented. These malignancies can develop in soft tissues of the oral cavity or centrally in the jaws. We report an 80-year-old patient with an injury corresponding to large B-cell non-Hodgkin lymphoma in the oral cavity, who has been treated with chemotherapy and radiotherapy, responding favorably to treatment. Likewise, in order to describe this infre-quent type of lesions of the oral cavity and to assess the prognosis they have, a literature review was performed. It is concluded that because of the prognosis of this type of lesions .
