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1.
Braz J Med Biol Res ; 38(6): 837-42, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15933776

ABSTRACT

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.


Subject(s)
Genes, MHC Class I/genetics , HLA-A Antigens/genetics , Histocompatibility Testing/methods , Reverse Transcriptase Polymerase Chain Reaction/methods , Alleles , Humans , Sequence Analysis, DNA/methods
2.
Braz. j. med. biol. res ; 38(6): 837-842, June 2005. ilus, tab
Article in English | LILACS | ID: lil-402664

ABSTRACT

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95 percent. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.


Subject(s)
Humans , Genes, MHC Class I/genetics , HLA-A Antigens/genetics , Histocompatibility Testing/methods , Reverse Transcriptase Polymerase Chain Reaction/methods , Alleles , Sequence Analysis, DNA/methods
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