ABSTRACT
Adina rubella Hance (AR), a plant native to Korea, has been used as traditional medicine for dysentery, eczema, intoxication, and external hemorrhages. Previous phytochemical studies of AR have reported several components, including terpenoids, phenolics, and alkaloids. The current study evaluated the anti-oxidative and anti-inflammatory activities and 5α-reductase inhibition of isolated compounds of AR leaves to find a potential therapeutic agent for benign prostatic hypertrophy (BPH). Repeated chromatographic isolation of an 80% acetone extract of AR leaves yielded seven phenolic compounds: caffeic acid (1), chlorogenic acid (2), methyl chlorogenate (3), quercetin-3-rutinoside (4), kaempferol-3-O-α-l-rhamnopyranosyl-(1â6)-ß-d-glucopyranoside (5), hyperoside (6), and grandifloroside (7). Compound 7 is a novel compound in AR. Caffeoyl derivatives 1-3 and 7 showed good anti-oxidative activities. In particular, caffeic acid (1) and grandifloroside (7) showed potent anti-inflammatory activities, and 7 also exhibited potent inhibitory activity against TNF-α and 5α-reductase. Our results show that the extract and grandifloroside (7) from leaves of AR might be developed as a source of potent anti-oxidative and anti-inflammatory agents and therapeutic agent for BPH.
Subject(s)
5-alpha Reductase Inhibitors/chemistry , 5-alpha Reductase Inhibitors/pharmacology , Phenols/chemistry , Phenols/pharmacology , Plant Leaves/chemistry , Rubiaceae/chemistry , 5-alpha Reductase Inhibitors/isolation & purification , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/pharmacology , Antioxidants/chemistry , Antioxidants/pharmacology , Cell Line , Cytokines/biosynthesis , Enzyme Activation/drug effects , Humans , Inhibitory Concentration 50 , Male , Mice , Molecular Structure , Phenols/isolation & purification , Plant Extracts/chemistry , Plant Extracts/pharmacology , Prostatic Hyperplasia/drug therapy , Prostatic Hyperplasia/metabolism , Prostatic Hyperplasia/pathologyABSTRACT
From the ethanol extract of Adina rubella;twelve compounds were isolated by silica gel and ODS col-umn chromatography.Their structures were identified by spectroscopic analysis as:5;7-dihydroxy-2-methyl-chromone-7-O-β-D-glucopyranoside(1);scopolin(2);(+)-lyoniresinol-3a-O-β-D-glucopyranoside(3);(6S;7R;8R)-7a-[(β-D-glucopyranosyl)oxy]lyoniresinol(4);harman-3-carboxylic acid(5);lyaloside(6);3-oxoquinovid acid(7);oleanolic acid(8);3-acetyl oleanolic acid(9);quinovic acid 3-O-β-D-glucopyranosyl-28-O-β-L-rham-nopyranosyl ester(10);quinovic acid 3-O-β-D-glucopyranosiyl-28-O-β-D-glucopyranosyl ester(11)and pyrocin-cholic acid 3-O-α-L-rhamnopyranosyl-28-[β-D-glucopyranosyl-(1 →6)-O-β-D-glucopyranosyl]ester(12). Compounds 3;4;6;8-10 were isolated from this plant for the first time.
ABSTRACT
[Objective] To screen the anticancer fraction from extract of root of Adina rubella Hance.[Methods]To separate the water extract from root of Adina rubella Hance by different solvens,then determine their anticancer activities of the different extracts in vitro with MTT method.[Results]The ethylacetate extract strongly inhibited cell proliferation of LS174T,and showed significant dosage-dependent response while the butanol extract and the chloroform extract showed low anticancer activities in vitro.[Conclusion]The ethylacetate extract is the principal anticancer extract from root of Adina rubella Hance.
ABSTRACT
Objective: To study the main components of polyphenols from the root of Adina rubelle Hance.Methods: Water extract from the root of Adina rubelle Hance was extracted with acetic ester,then separated by silicage column with differentiation by thin-layer chromatography.Thirdly,the purified fraction was analyzed by GC-MS.Results: Two components were purified,they were catechins compounds.Conclusion: Polyphenols from the root of Adina rubelle Hance were mainly consisted of catechins.
