Impact of human leucocyte antigen class II polymorphism on anti-red blood cell antibody development: Correlations and indications.

BACKROUND AND OBJECTIVES: Blood transfusion therapy is vital for many patient groups. They can cause many complications, and the development of anti-red blood cell (RBC) antibodies is of significant importance. Molecules of class II human leucocyte antigens (HLA) are one of the several factors that influence antibody development in patients. MATERIALS AND METHODS: In this study, we investigated 108 patients who developed antibodies against different erythrocyte antigens and 115 patients on multiple transfusion therapies who did not develop anti-RBC antibodies. The HLA loci HLA-DRB1 and HLA-DQB1 were typed using commercial molecular assays routinely used in HLA laboratories. RESULTS: An increased frequency of the HLA-DRB1*04 allele group was observed in patients who developed antibodies. Additionally, HLA-DRB1*09 was also significant for anti-E development and in patients with multi-specific alloimmunization. It was found that the HLA-DRB1*07 allele group is associated with antibodies to antigents of the Rh and MNS systems but also lacks an association with anti-K development. The HLA-DRB1*11 and -DRB1*01 allele groups displayed a protective mechanism for anti-E development, similar to that of HLA-DQB1*02 for anti-K. CONCLUSION: There is an association between various HLA class II alleles and anti-RBC development.

Treatment of acne vulgaris using 1,565 nm non-ablative fractional laser in combination with isotretinoin and pricking blood therapy.

OBJECTIVE: We aimed to evaluate the efficacy and safety of the 1,565 nm non-ablative fractional laser (NAFL) in combination with isotretinoin and pricking blood therapy (PBT) for treatment of AV. METHODS: A retrospective analysis of 60 patients with moderate-to-severe AV was performed. Four groups (n = 15) were evaluated: 1,565nm NAFL alone, oral isotretinoin alone, double therapy (NAFL + isotretinoin) and triple therapy (NAFL + isotretinoin + PBT). RESULTS: The triple therapy showed the highest improvement rate of inflammatory papules and boxcar atrophic scars. The patients receiving oral isotretinoin alone, double or triple therapy showed a significant decrease in volume of boxcar atrophic scars. The NAFL alone, double or triple therapy significantly decreased index of hemoglobin. Furthermore, the triple therapy significantly decreased indexes of hemoglobin and red areas as compared to the other three treatments. All four treatments significantly decreased indexes of pore sizes and wrinkles. CONCLUSION: This study showed that the triple therapy is effective for treatment of AV, which is worthy of further evaluation and investigation.

Perceptions About Blood Transfusion Therapy Among the General Public and Healthcare Professionals in the Qassim Region of Saudi Arabia.

PURPOSE: Blood transfusion is a conventional therapeutic procedure; however, the perceptions of general public and healthcare professionals (HCPs), especially physicians and nurses, remain unclear, although the insights of HSPs may affect the treatment decision. This study aimed to assess the awareness of HCPs and the public about blood transfusion risks and consent in Qassim region of Saudi Arabia, to uncover the factors that may influence such perceptions. PATIENTS AND METHODS: This study used two different closed questionnaires that were distributed electronically between February and March 2018 among the population and HCPs in Qassim region. RESULTS: A total of 400 general public participants and 135 HCPs completed the survey. Among the surveyed participants, 70% believed that blood transfusion therapy was safe. The perceived risk of human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) was the highest among all complications (74%). Furthermore, 88.2% of respondents were willing to accept a blood transfusion as a therapeutic measure, primarily from a first-degree relative, although the remaining 11.8% rejected the idea of a transfusion due to fear of medical error. From the HCP survey, 80% were previously involved in a blood transfusion therapy consent process. HCPs typically reported explaining the benefits, risks, and alternatives described in the consent form (74.1%, 67.4%, and 53.3%, respectively). CONCLUSION: Our results indicated that despite the current high level of acceptance and knowledge regarding blood transfusions, additional educational efforts remain necessary to increase public awareness of blood transfusion therapy.

Medidas de distanciamento social e demandas para reorganização dos serviços hemoterápicos no contexto da Covid-19 / Social distancing measures and demands for the reorganization of hemotherapy services in the context of Covid-19

Resumo O artigo tem por objetivo discutir as consequências das medidas de distanciamento social sobre a disponibilidade de sangue e a organização dos serviços hemoterápicos no início da pandemia de Covid-19 no Brasil. A partir do acesso às notícias publicadas no mês de abril de 2020 nos sites das Hemorredes estaduais do país e organizadas em matriz Excel, foram elaborados quadros-síntese e descrição de resultados. A situação crítica de abastecimento de sangue, em especial para alguns tipos sanguíneos, foi constatada em muitos estados, devido à circulação do novo coronavírus. A adoção de medidas de distanciamento social e a manutenção das demandas por transfusão para tratamentos, urgência e emergência, requereram a implantação e a implementação de estratégias e ações para reorganização dos serviços. Medidas de proteção foram incorporadas, fluxos alterados e rotinas estabelecidas. Este estudo evidencia o quanto a situação epidemiológica da Covid-19 e as medidas necessárias para o seu controle influenciaram sobre a situação dos estoques e disponibilidade de sangue, e, demandaram, mudanças na organização dos serviços hemoterápicos a fim de assegurar a proteção, mitigar os riscos de propagação do vírus e garantir o suprimento de sangue para o atendimento às necessidades do sistema de saúde.

Abstract The article aims to discuss the consequences of social distancing measures on the availability of blood and organization of blood therapy services at the beginning of the Covid-19 pandemic in Brazil. News published in April 2020 on the websites of the country's state Blood Service Networks were consulted and organized in an Excel spreadsheet, presented in summary charts, and descriptions of results were prepared. A critical situation of blood supply, especially of some blood types, has been observed in many states. This situation is influenced by the circulation of the new coronavirus. The adoption of social distancing measures associated with unchanged transfusion demands for outpatient, urgency and emergency care required the implementation of strategies and actions for the reorganization of the services. Protection measures were incorporated, flows were changed and new routines were established. This study shows the extent to which the epidemiological situation of Covid-19 and the necessary measures for its control influenced the stocks and availability of blood. Changes in the organization of blood therapy services were fundamental in order to ensure protection, mitigate the risks of spreading the virus, and ensure the blood supply to meet the needs of the health system.

[The medical research of "chicken blood therapy"].

In 1952, inspired by the "tissue therapy" which was being carried out nationwide at that time, Yu Changshi, a folk science enthusiast, proposed "chicken blood therapy" . In 1959, it was supported as an important achievement in technological innovation and technological revolution movement. In the following years, some researchers and many hospitals organized by the leaders of Jing'an District of Shanghai successively carried out the animal experiment, the first phase clinical experiment and the second phase clinical experiment of this therapy, and this therapy was also widely spread during this period.However, the expert meeting in 1965 gave it a basically negative conclusion on the basis of insecurity and poor long-term effect.

[Exploration of academic ideas on pricking blood therapy of Rumen Shiqin].

Through in-depth excavation and analysis of the pricking blood therapy mentioned in Rumen Shiqin, it is found that ZHANG Zi-he's pricking blood theory is mainly derived from Huangdi Neijing. Innovative development has been carried out on the basis of "brave innovation and careful verification", and at the same time, it has been practiced and summarized clinically to form its own unique pricking blood therapy. This article systematically summarizes formation of the theory, clinical application, characteristics, and influence on later generations of pricking blood therapy in Rumen Shiqin, with a view to inspiring scholars to have a more comprehensive understanding on the pricking blood therapy in Rumen Shiqin, and providing new ideas and reference for clinical application.

Exploration of academic ideas on pricking blood therapy of / 中国针灸

Through in-depth excavation and analysis of the pricking blood therapy mentioned in , it is found that -'s pricking blood theory is mainly derived from . Innovative development has been carried out on the basis of "brave innovation and careful verification", and at the same time, it has been practiced and summarized clinically to form its own unique pricking blood therapy. This article systematically summarizes formation of the theory, clinical application, characteristics, and influence on later generations of pricking blood therapy in , with a view to inspiring scholars to have a more comprehensive understanding on the pricking blood therapy in , and providing new ideas and reference for clinical application.

Hematological, biochemical, and blood gas alterations of goat whole blood stored in CPDA-1 and CPD/SAG-M plastic bags / Avaliação dos parâmetros hematológicos, bioquímicos e hemogasométricos do sangue caprino armazenado em bolsas CPDA-1 e CPD/SAG-M

ABSTRACT: The aim of this study was to evaluate the hematological, biochemical, and blood gas alterations of goat whole blood stored in different blood bags. Seven male, adult, crossbreed goats were used, weighing 62±1.8 kg. Nine hundred milliliters of whole blood from each animal was collected and stored in blood bags (450 ml in each), CPDA-1 (citrate phosphate dextrose-adenine) and CPD/SAG-M (citrate phosphate dextrose with saline-glucose-mannitol) as additive solutions, and kept refrigerated (2-4 ºC) for 42 days. Blood samples were collected from the plastic bags at baseline (T0) and after seven, 14, 21,28, 35, and 42 days for hematological, biochemical, blood gas, and microbiological evaluations. Free hemoglobin, degree of hemolysis, lactate, and pO2were increased in both bags, whereas hydrogen potential (pH) and the total hemoglobin concentration decreased overtime(P<0.05). The red blood cell count, glucose, sodium, and potassium remained stable, compared to the baseline. The CPD/SAG-M bag presented a lower red cell count, globular volume, total hemoglobin, and sodium, and a higher degree of hemolysis and plasma hemoglobin, compared with the CPDA-1 bag. The whole goat blood remained viable for therapeutic use; although, there were some important changes in the variables of the 42-day stored blood in relation to fresh blood (T0). We concluded that the CPDA-1 bag is more suitable for use in the storage of goat blood because of its lower commercial value.

RESUMO: O objetivo deste estudo foi avaliar as alterações hematológicas, bioquímicas e hemogasométricas do sangue total de caprinos armazenado em diferentes bolsas de sangue. Foram utilizados sete caprinos machos, adultos, sem raça definida, pesando 62±1,8kg. Coletou-se 900 mL de sangue total de cada animal, em duas bolsas plásticas tipos CPDA-1 (citrato-fosfato-dextrose-adenina) e CPD/SAG-M (citrato-fosfato-dextrose com solução aditiva de salina-glicose-manitol), armazenadas sob refrigeração (2-4 °C) durante 42 dias. Amostras de sangue foram coletadas das bolsas no início do estudo (T0) e depois de sete, 14, 21, 28, 35 e 42 dias, para realização de análises hematológicas, bioquímicas, hemogasométricas e microbiológicas. Nas duas bolsas houve aumento da concentração de hemoglobina plasmática, grau de hemólise, lactato, pO2 e redução de hemoglobina total e pH (p<0,05), porém não houve variação no número de hemácias, glicose, sódio e potássio quando comparado ao momento basal (T0). A bolsa CPD/SAG-M apresentou menor número de hemácias, volume globular, concentração de hemoglobina total e sódio, e maiores valores de grau de hemólise e hemoglobina plasmática em relação a bolsa CPDA-1 (P<0,05). Apesar de serem constatadas alterações em importantes variáveis do sangue caprino armazenado durante 42 dias em relação ao sangue fresco (T0), o sangue total caprino permaneceu viável para uso terapêutico. A análise comparativa entre os dois tipos de bolsa revelou uma similaridade de resultados para a grande maioria das variáveis estudadas, portanto a bolsa CPDA-1 pode ser considerada a mais indicada para uso na espécie caprina exclusivamente devido ao seu menor valor comercial.

Clinical Observation of Wrist-ankle Acupuncture plus Bloodletting Cupping for Diabetic Peripheral Neuropathies / 上海针灸杂志

Objective To observe the clinical efficacy of wrist-ankle acupuncture plus bloodletting cupping in treating diabetic peripheral neuropathies (DPN). Method A total of 120 patients were randomized into two groups by using the random number table. Sixty cases in the treatment group were intervened by wrist-ankle acupuncture plus bloodletting cupping; sixty cases in the control group were treated with Mecobalamin tablets. The clinical efficacies were evaluated and electroneurogram was tested after 28-day treatment. Result The total effective rate was 95.0% in the treatment group versus 88.3% in the control group. The clinical efficacy of the treatment group was significantly superior to that of the control group (P<0.05). The treatment group was significantly better than the control group in comparing the major symptoms and signs including numbness, pain, and weakness in limbs (P<0.05); there were no significant between-group differences in comparing symptoms including cold limbs, short of breath and unwilling to speak, lassitude, and thirsty with desire to drink (P>0.05). The treatment group had better effect than the control group in comparing the motor conduction velocity of superficial peroneal nerve and sensory conduction velocity of common peroneal nerve (P<0.05). Conclusion Wrist-ankle acupuncture plus bloodletting cupping is effective in treating DPN, and it benefits the recovery of motor and sensory function of the impaired peripheral nerves.

Hemoterapia: um desafio no cotidiano da equipe de enfermagem / Hemotherapy: a challenge in the daily team of the nursing team / Hemoterapia: un desafío en el cotidiano del equipo de enfermería

Objetivo: identificar o conhecimento de uma equipe de enfermagem sobre o processo transfusional. Método:estudo descritivo, exploratório e transversal, de abordagem qualiquantitativa, realizado no Hospital EscolaLuiz Gioseffi Jannuzzi, com 57 profissionais da equipe de enfermagem. Os dados foram coletados a partir deum questionário semiestruturado e, posteriormente, analisados de forma descritiva. Resultados: mostram umconhecimento pouco significativo por parte da equipe de enfermagem sobre os cuidados pré e diante de umareação transfusional. Conclusão: destaca-se a necessidade desses profissionais terem educação permanente arespeito do tema, além de treinamento em serviço, para uma conduta mais adequada mediante qualquerintercorrência que possa acontecer, visando a um melhor atendimento e sem riscos maiores para o paciente.(AU)

Objective: to identify the knowledge of a nursing team about the transfusion process. Method: this is a descriptive, exploratory, and transversal study, with a qualitative approach, performed at the Luiz Gioseffi Jannuzzi School Hospital, with 57 professionals from the nursing team. The data were collected from a semistructured questionnaire and, subsequently, analyzed in a descriptive way. Results: a little knowledge by the nursing team is shown the care before and in the transfusion reaction. Conclusion: the need for these professionals to have permanent education on the subject, in addition to in-service training, for an adequate conduct through any intercurrence that may happen, aiming at a better care and without greater risks for the patient.(AU)

Objetivo: identificar el conocimiento de un equipo de enfermería sobre el proceso transfusional. Método: estudio descriptivo, exploratório y transversal, de enfoque cualitativo y cuantitativo, realizado en el Hospital Escuela Luiz Gioseffi Jannuzzi, con 57 profesionales del equipo de enfermería. Los datos fueron recogidos a partir de un cuestionario semi-estructurado y, posteriormente analizados de forma descriptiva. Resultados: muestran un conocimiento poco significativo por parte del equipo de enfermería sobre los cuidados pre y frente una reacción transfusional. Conclusión: se destaca la necesidad de esos profesionales tener educación permanente al respecto del tema, además de entrenamiento en servicio, para una conducta más adecuada mediante cualquer intercurrencia que pueda acontecer, visando a un mejor atendimiento y sin riesgos mayores para el paciente.(AU)

Preterm white matter brain injury is prevented by early administration of umbilical cord blood cells.

Infants born very preterm are at high risk for neurological deficits including cerebral palsy. In this study we assessed the neuroprotective effects of umbilical cord blood cells (UCBCs) and optimal administration timing in a fetal sheep model of preterm brain injury. 50 million allogeneic UCBCs were intravenously administered to fetal sheep (0.7 gestation) at 12h or 5d after acute hypoxia-ischemia (HI) induced by umbilical cord occlusion. The fetal brains were collected at 10d after HI. HI (n=7) was associated with reduced number of oligodendrocytes (Olig2+) and myelin density (CNPase+), and increased density of activated microglia (Iba-1+) in cerebral white matter compared to control fetuses (P<0.05). UCBCs administered at 12h, but not 5d after HI, significantly protected white matter structures and suppressed cerebral inflammation. Activated microglial density showed a correlation with decreasing oligodendrocyte number (P<0.001). HI caused cell death (TUNEL+) in the internal capsule and cell proliferation (Ki-67+) in the subventricular zone compared to control (P<0.05), while UCBCs at 12h or 5d ameliorated these effects. Additionally, UCBCs at 12h induced a significant systemic increase in interleukin-10 at 10d, and reduced oxidative stress (malondialdehyde) following HI (P<0.05). UCBC administration at 12h after HI reduces preterm white matter injury, via anti-inflammatory and antioxidant actions.

Abdominal fat suspension device for maintaining normal cardiorespiratory function in patients undergoing conscious sedation during surgery: a feasibility study.

Obese patients undergoing conscious-sedation surgery have increased perioperative morbidity because their excess abdominal tissue limits diaphragmatic excursion. We describe a simple device that might help attenuate this risk. We created a noninvasive suction device for abdominal suspension. By lifting the burden of excess weight, this device should decrease respiratory effort. To test the feasibility of excess weight removal in relieving cardiac stress, we tested 22 supine, healthy, normal-weight subjects by measuring their heart rates with and without a 13-kg tissue model on their abdomen to simulate excess weight. There was no significant difference in blood oxygen saturation before and after weight removal (P=0.318). However, the decrease in heart rate was significant (P <0.0001; paired 2-sample, one-tailed t test), which implies decreased respiratory effort. This result suggests the possibility that abdominal mass suspension in obese patients is associated with decreased respiratory effort.

Thrombus formation during percutaneous closure of an atrial septal defect with an Amplatzer septal occluder.

Percutaneous closure of an ostium secundum-type atrial septal defect is typically a safe and effective therapeutic option in the presence of significant shunting or paradoxical embolism. Infrequently, however, periprocedural sequelae occur.Herein, we report the cases of 2 patients, each of whom underwent transcatheter closure of an atrial septal defect with the use of an Amplatzer Septal Occluder under transesophageal echocardiographic guidance. In both patients, acute thrombi formed periprocedurally, despite preprocedural anticoagulation. In patient 1, the infusion of unfractionated heparin for 24 hours prevented the recurrence of thrombus; in patient 2, the thrombus was isolated under the arm of the occluder, and unfractionated heparin was infused. Both patients were asymptomatic and without detectable thrombus after the procedure and at follow-up. These reports highlight a rare early sequela and the importance of transesophageal echocardiographic monitoring during the percutaneous closure of an atrial septal defect.

Effect of Pricking Blood Therapy on Behavior and Gene Expression in a Rat Model of Migraine / 针灸推拿医学（英文版）

Objective: To investigate the effect of pricking blood therapy on behavior and brainstem c-fos and c-jun gene expression in migraine rats. Methods: A rat model of migraine was made with nitroglycerol. The rats were treated by pricking blood. Rat behavior was observed. Brainstem c-fos and c-jun gene expression was examined by immunohistochemistry. Results: Ear redness improved significantly, the number of head scratchings decreased obviously (P<0.05) and c-fos and c-jun expression was reduced markedly (P<0.01) in the treatment group after pricking blood compared with the model group and the blank group. Conclusion: Pricking blood treatment can improve behavior indices and reduce c-fos and c-jun positive expression in migraine rats.

Therapeutic Effect Observation on Treatment of Acne with Acupuncture plus Moving Cupping and Blood-letting / 针灸推拿医学（英文版）

Objective: To observe the therapeutic effect on treatment of acne with acupuncture plus moving cupping and blood-letting. Method: Sixty acne cases were randomized into the treatment group for combined acupuncture and moving cupping and blood-letting and control group for acupuncture alone. The therapeutic effects of the cases in the two groups were observed after a 30-day treatment. Result: The total effective rate was significantly higher in the treatment group than in the control group (P<0.05). Conclusion: The combined acupuncture and moving cupping and blood-letting can effectively increase the effective rate in the treatment of acne.

Clinical Research of Invigorating the Kidney and Activating Blood Therapy on Treating Osteoarthritis Patients in the Pattern of Kidney Deficiency / 中国中医药信息杂志

Objective To explore the clinical effiicacy and effect on cytokines of invigorating the kidney and activating blood fomula on treating osteoarthritis patients in the pattern of kidney deficiency. Methods 113 patients with knee osteoarthritis were randomly divided into two groups including 56 cases of treatment group and 57 cases of control group, which were treated by invigorating the kidney and activating blood fomula and combinaton of Celecoxib and Glucosamine respectively. The clinical effiicacy of two groups were investigated by Womac osteoarthritis index and the average severity of OA index. The changes of serum inflammatory cytokines TNF-? and IL-6 were detected by ELISA. Results The total efficiency of the treatment group was 89.3% and better than the control group (P 0.05) compared with control group. The serum TNF-?, IL-6 levels of treatment group significantly decreased after treatment in treatment group (P

Treatment of Stye by Auricular Point Blood-letting Therapy: A Report of 85 Cases / 针灸推拿医学（英文版）

Eighty-five cases of stye were treated by pricking and extruding some blood in auricular points Eye 1 and Eye 2. After 5 treatments, 63 cases were cured, 17 cases obtained improvement, 5 cases obtained no effects, and the total effective rate is 94.1%.

Clinical Observation on Treatment of Eczema by Acupuncture plus Collateral-pricking and Cupping: A Report of 54 Cases / 针灸推拿医学（英文版）

Patients with eczema were treated by acupuncture and collateral-pricking and cupping,according to the principle of clearing away heat and eliminating dampness, enriching the blood and moistening dryness. Main acupoints were Quchi (LI 11), Baichongwo (Ex-LE), Hegu (LI 4), Sanyinjiao (SP 6), Xinjian (LR 2) and Neiting (ST 44). Among 54 patients, 23 cases recovered, 24 cases got effectiveness and 7 case had no change after the treatment. The curative effect rate was 87.0%.

Treatment of Post-herpetic Neuralgia with Acupuncture plus Cupping: A Report of 56 Cases / 针灸推拿医学（英文版）

Fifty-six middle and aged post-herpetic neuralgia patients were treated with Jiaji (Ex- B 2) of the correspondingly affected segments plus Ashi points. The major points were treated with even reinforcing-reducing manipulation and the auxiliary points were treated by puncturing subcutaneously around the skin lesion with reducing manipulation. Afterward the three-edged needle was used to insert into the tenderness spots rapidly and remove immediately for 3-5 consecutive times around the spot plus flash-fire cupping. The result showed recovery in 36 cases; marked effect in 17 cases; no effect in 3 cases and the total effective rate was 94.6%.

Effect of Reinforcing Kidney and Activating Blood Therapy on Apoptosis of Rat Models with Endometriosis / 中医杂志

Objective To study the mechanism of reinforcing kidney and activating blood therapy for the apoptosis of endometriosis.Methods Female SD rats aged 8~12weeks were grouped as model group,western medicine group,Chinese medicine group and integrated Chinese and western medicine group,with 15 in each group.The light microscope,electron microscope,TUNEL method,and immunohistochemical method were adopted to observe the pathomorphology,positive rate of apoptotic cells and the positive expression of apoptosis-regulating gene bcl-2 and bax.Results The apoptosis of endometriosis tissues in the integrated Chinese and western medicine group was the most obvious result with the highest apoptosis index,and the positive expression of apoptosis regulating gene bcl-2 was downward regulated and that of apoptosis regulating gene bax upward regulated,so bcl-2/bax decreased.Then it was Chinese medicine group,western medicine group,and model group.Conclusion The occurrence of endometriosis is related with the lowering of ability of apoptotic cells.Chinese medicines with the functions to reinforce kidney and activate blood can promote the ability of apoptotic cells in endometriosis to quicken the atrophy and regression of endometriosis tissues.