ABSTRACT
Healthy adult mice were inflicted with combined injury of gamma radiation(4 Gy from a 60Co source) and burns (15% TBSA third degree).From 6 daysprior to to 8 days after the injury.TEP-87,which consists of zinc,copper andiron in the dosage appropriate to the physiological requirement as well as lycine,vitemins B1,B2,C and D,folic acid and malt,was administered.It was found that the protective effect of this preparation on the marrow cells of the myeloblastic line of the animals inflicted with either simple radiation injury or combined injury of radiation and burns was obvious even on the first day after injury.Meanwhile,the total number of white blood cells was maintained at a relatively high level.On the 8th day after injury,accelerated rate of recovery of the mice was observed.No favorable effect could be revealed on the uncleated cells of the bone marrow,or on the positive rate of esterase which is a nonspecific marker of T-lymphocytes.The protective effects of TEP-87 on the hemopoietic function of the mice with combined radiation-burn injury might be due to the direct stimulation of zinc,copper and iron on the proliferation of the hemopoietic stem cells.
ABSTRACT
Fifty-seven rats were randomly divided into 6 groups. The ventricular cell action potentials of combined irradiation-burn injury in situ were compared with that of radiation or burn injury and the recovery process from the effect of combined injury on myocardium was observed by floating microelectrode. The results obtained were as follows.Similar to that of radiation or burn injury, both the depolarization and repo-larization of action potential were also changed by combined radiation-burn injury significantly, but there are some peculiar characteristics of its own. Twenty-four hours after combined injury, the amplitude of action potential (APA, -13.6%), maximum depolarization rate, (Vmax, -41.2%) , as compared with that of cort-rol. The changes of heart rate were different. In burn group.it increased by+9.8%; in radiation group, it decreased by -17.4%, however, it appoximated to control level in combined group at 24 hours after injury. Three days after combined injury, the HR was decreased by -12%, after 5 days, it only recovered slightly. Combined injury decreased the mean repolarization rate(MRR, -26.3%--13.2%)and prolonged the duration of action potential (APD), especially APD50 and APD90,( +33.7%-+ 22.9%) and( + 6.9%- + 18%)respectively.These results suggest that the effects of combined injury are not a simple algebraic sum of radiation and burn injury. In some aspects, such as Vmax, there is synergism between the effects of radiation and burn injury, but in other cases, e.g., HR, these effects seem to be counterblanced each other. These changes in ventricular action potentials of the combined radiation-burn injury are also a reversible process. In general, the earlier the response of injury (such as in APA and Vmax) appears, the faster the recovery occurs and vice versa (such as in Vmax and HR) .
