ABSTRACT
Objective To evaluate the function of Hybrid capture 2 (HC2) human papillomavirus (HPV) test,liquid-based cytology test (LCT),HPV serial test,LCT serial test and HPV-LCT parallel test for cervical high-grade lesions screening and to guide in selecting the scheme of cervical cancer screening.Methods HPV test,LCT,and colpescopic biopsy were performed in 545 women treated in Centrale Hospital of Klamayi from Sep.2009 and Dec.2011.The pathology results were the final diagnosis to evaluate the value of each approach for screening cervical cancer.Results Of 545 patients, 10 had cervical cancer.41 had cervical intraepithelial neoplasia CIN3,69 had CIN2,and 120 had CIN 1.Positive rate of high risk HPV was 64.22 ％.The sensitivity,specificity,accuracy,positive pre-value,and negative pre-value of HPV test for detecting high-grade cervical lesions were 95.83 ％,84.71％,88.48 ％,30.68 ％,99.12 ％,respectively.The values of LCT were 84.20 ％,89.53 ％,85.53 ％,36.69 ％,91.76 ％,respectively.The values of LCT serial test were 81.03 ％,95.96 ％,78.33 ％,40.69 ％,92.92 ％,respectively.The values of HPV serial test were 81.03 ％,95.26 ％,78.33 ％,42.15 ％,99.52 ％,respectively.The values of HPV-LCT parallel test were 99.20 ％,81.53 ％,90.03 ％,32.09 ％,99.33 ％,respectively.Conclusion HPV-LCT parallel test can significantly improve the detection rate of cervical cancer screening.
ABSTRACT
Objective To evaluate the application of different assays for detection of human papillomavirus(HPV)in diagnosis of high grade cervical lesions.Methods Two hundred subjects with abnormal thinprep liquid-based cytology test(TCT)Resultswere selected for HPV DNA detection by hybrid capture 2(HC-Ⅱ) and Methodsbased on PCR including flow-through hybridization and gene chip (HybriMax),real-time fluorescent quantitative PCR(FQ-PCR)and flow fluorescent hybridization assay.Cytopathological Resultswere used as gold standards to evaluate the test performance of the above assays for diagnosing cervical intraepithelial neoplasia(CIN)≥Ⅱ. SPSS 13.0 software was used for statistical analysis.Results HPV DNA positive rates of 200 samples by HybriMax,FQ-PCR,flow fluorescent hybridization assay and HC-Ⅱ were 72.5%(145/200),71.5%(143/200),70.0%(140/200)and 69.0%(138/200),respectively,and the differences were not statistically si(g)nificant(x2 =0.252,0134,0.012 and 0.027,P ＞ 0.05).The sensitivity,Youden index and negative predictive value of the above assays were statistically different(x2 =7.923,7.819 and 8.108,P ＜0.05).Conclusion HC-Ⅱ is superior to PCR Methodsin diagnosis of CIN Ⅱ and above.
