ABSTRACT
Abstract INTRODUCTION: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. METHODS: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. RESULTS: The rotating method was easier, faster, and more sensitive than the others with 100% specificity. CONCLUSIONS: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Subject(s)
Humans , Animals , Trypanosoma cruzi/isolation & purification , Centrifugation/methods , Chagas Disease/diagnosis , Parasitemia/diagnosis , Capillary Tubing , Hematocrit/methods , Sensitivity and Specificity , Chagas Disease/parasitology , Chagas Disease/blood , Parasitemia/parasitology , Clinical Laboratory ServicesABSTRACT
RESUMEN Objetivo Establecer la correlación entre los métodos de Wintrobe y de micro-hematocrito Métodos Se tomaron 407 pacientes asistentes a un laboratorio de tercer nivel a quienes en forma simultánea se les realizó las dos pruebas en estudio. Resultados Mediante el un método estadístico de regresión lineal se encontró un coeficiente de correlación de 0,99. Conclusión Ambos métodos pueden contribuir al análisis clínico de los pacientes a quienes se les solicitó la prueba con fines de diagnóstico, control y seguimiento de diferentes patologías.(AU)
ABSTRACT Objective To establish the correlation between the methods of Wintrobe and micro-hematocrit Methods 407 patients attendees were taken laboratory to third level simultaneo usly tests were performed in the study. Results Using a statistical method of linear regression correlation coefficient of 0,99 was found. Conclusion Both methods can contribute to the clinical analysis of patients who were asked to test for diagnosis or control of different pathologies.(AU)
Subject(s)
Humans , Blood Sedimentation , Hematocrit/methods , Hematologic Diseases/diagnosis , Linear Models , Epidemiology, DescriptiveABSTRACT
The objective of the research was to evaluate the mortality cause in horses on a farm in the central area of the state of Rio Grande do Sul, Brazil. Blood samples of nine animals were analysed using the microhematocrit technique and stain of buffy coast where in three samples the presence of Trypanosoma evansi was detected. The clinical signs presented by the animals were anemia, edema of the ventral parts of the body and incoordenation of the subsequent members. The techniques used are of fundamental importance in parasitologics selection samples to increase the chances of finding the protozoan.
O objetivo deste trabalho foi avaliar a causa de mortalidade em cavalos de uma propriedade na região central do Rio Grande do Sul. Amostras de sangue de nove animais foram analisadas utilizando-se a técnica do micro-hematócrito e colorações da capa flogística sendo detectada a presença de Trypanosoma evansi em três amostras. Os sinais clínicos apresentados pelos animais foram anemia, edema das partes ventrais do corpo e incoordenação dos membros posteriores. As técnicas empregadas são de fundamental importância na triagem parasitológica a fim de aumentar as chances de observação dos protozoários.
ABSTRACT
The objective of the research was to evaluate the mortality cause in horses on a farm in the central area of the state of Rio Grande do Sul, Brazil. Blood samples of nine animals were analysed using the microhematocrit technique and stain of buffy coast where in three samples the presence of Trypanosoma evansi was detected. The clinical signs presented by the animals were anemia, edema of the ventral parts of the body and incoordenation of the subsequent members. The techniques used are of fundamental importance in parasitologics selection samples to increase the chances of finding the protozoan.
O objetivo deste trabalho foi avaliar a causa de mortalidade em cavalos de uma propriedade na região central do Rio Grande do Sul. Amostras de sangue de nove animais foram analisadas utilizando-se a técnica do micro-hematócrito e colorações da capa flogística sendo detectada a presença de Trypanosoma evansi em três amostras. Os sinais clínicos apresentados pelos animais foram anemia, edema das partes ventrais do corpo e incoordenação dos membros posteriores. As técnicas empregadas são de fundamental importância na triagem parasitológica a fim de aumentar as chances de observação dos protozoários.