Long-term grazing reduces soil fungal network complexity but enhances plant-soil microbe network connectivity in a semi-arid grassland.

Grazing plays a significant role in shaping both aboveground vegetation and belowground microbial communities in arid and semi-arid grasslands, which in turn affects ecosystem functions and sustainability. Therefore, it was essential to implement effective grazing management practices to preserve ecological balance and support sustainable development in these delicate environments. To optimize the traditional continuous grazing policy, we conducted a 10-year seasonal grazing experiment with five treatments in a typical grassland in northern China: no grazing (NG), continuous summer grazing (CG), and three seasonal grazing treatments (G57 in May and July, G68 in June and August, and G79 in July and September). Our study found that although grazing reduced plant community biomass, G68 treatment maintained high plant height and community diversity (P < 0.05). Grazing did not affect soil bacterial and archaeal alpha diversity, but CG treatment reduced soil fungal diversity (P < 0.05). CG reduced the archaeal network's vertices (which represent microbial taxa, OTUs) and connections (ecological interactions between taxa), but seasonal grazing increased its complexity. Furthermore, grazing did not change bacterial networks but enhanced cross-domain interactions (relationships between different biological groups) of plant-soil fungi and plant-soil archaea. Overall, we used the Mantel test to find that soil microbial diversity was positively correlated with soil physicochemical properties rather than plant community characteristics after grazing. These findings are beneficial for the optimization of sustainable grassland management policies and the protection of plant and soil biodiversity.

Grazing practices affect soil microbial networks but not diversity and composition in alpine meadows of northeastern Qinghai-Tibetan plateau.

Livestock grazing is the primary practice in alpine meadows and can alter soil microbiomes, which is critical for ecosystem functions and services. Seasonal grazing (SG) and continuous grazing (CG) are two kinds of different grazing practices that dominate alpine meadows on the Qinghai-Tibetan Plateau (QTP), and how they affect soil microbial communities remains in-depth exploration. The present study was conducted to investigate the effects of different grazing practices (i.e., SG and CG) on the diversity, composition, and co-occurrence networks of soil bacteria and fungi in QTP alpine meadows. Soil microbial α- and ß-diversity showed no obvious difference between SG and CG grasslands. Grazing practices had little impact on soil microbial composition, except that the relative abundance of Proteobacteria and Ascomycota showed significant difference between SG and CG grasslands. Soil microbial networks were more complex and less stable in SG grasslands than that in CG grasslands, and the bacterial networks were more complex than fungal networks. Soil fungal diversity was more strongly correlated with environmental factors than bacteria, whereas both fungal and bacterial structures were mainly influenced by soil pH, total nitrogen, and ammonium nitrogen. These findings indicate that microbial associations are more sensitive to grazing practices than microbial diversity and composition, and that SG may be a better grazing practice for ecological benefits in alpine meadows.

Effects of seasonal grazing on plant and soil microbial diversity of typical temperate grassland.

Biodiversity is the decisive factor of grassland ecological function and process. As the most important human use of grassland, grazing inevitably affects the grassland biodiversity. However, comprehensive studies of seasonal grazing on plant and soil bacterial, archaeal and fungal diversity of typical temperate grassland are still lacking. We examined the impact of seasonal grazing, including no-grazing (NG), continuous grazing (CG), grazing in May and July (G57), grazing in June and August (G68), and grazing in July and September (G79) on grassland plant and soil microbial diversity based on a long-term field grazing experiment. The results showed that the aboveground plant biomass (AGB) of the seasonal grazing plots was significantly higher than that of the CG plots. Compared with NG, CG increased significantly the Margalef richness index of plant community, while did not significantly change the Shannon, Simpson and Pielou evenness of plant community. Grazing changed the composition and biomass of dominant vegetation. Long-term grazing decreased the proportion of Leymus chinensis (Trin.) Tzvel. and increased the proportion of Cleistogenes squarrosa (Trin.) Keng. There was no significant change in the Shannoneven, Shannon and Coverage indices of soil bacteria, archaea and fungi between NG and the grazing plots. But the Chao index of soil fungi in G57, G68 and G79 and archaea in G57, G79 was significantly higher than that in CG. The results of correlation analysis showed that the plant diversity in the CG plots was significantly negatively correlated with the soil bacterial diversity. The plant richness in the G57 and G68 plots was significantly positively correlated with the soil archaea richness. Our study showed that seasonal grazing was a sustainable grazing management strategy for maintaining typical grassland plant and soil microbial diversity in northern of China.

Response of forage nutrient storages to grazing in alpine grasslands.

Forage nutrient storages can determine livestock size and husbandry development. There is insufficient research on the response of forage nutrient storages to grazing and related driving mechanisms in alpine grasslands, especially on the Tibetan Plateau. This study conducted a grazing experiment in three alpine grassland sites along an elevation gradient (two warm-season pastures and one cold-season pasture; two alpine steppe meadow sites and one alpine meadow) of Northern Tibet. Different types of alpine grassland ecosystems, at least for forage nutrient storages, may have different responses to grazing. Warm-season grazing significantly reduced crude protein (CP) storage, acid detergent fiber (ADF) storage, and neutral detergent fiber (NDF) storage of high-quality forage by 53.29, 63.82, and 63.26%, respectively, but cold-season grazing did not significantly alter the CP, ADF and NDF storages of high-quality forage. Warm-season grazing significantly reduced CP, ADF, NDF, crude ash (Ash), ether extract (EE) and water-soluble carbohydrate (WSC) storages of the plant community by 46.61, 62.47, 55.96, 64.94, 60.34, and 52.68%, and forbs by 62.33, 77.50, 73.69, 65.05, 57.75, and 62.44% in the alpine meadow site but not the alpine steppe meadow site, respectively. Plant species and phylogenetic diversity had different relationships with forage nutrient storages. The elevation distribution of forage nutrient storages under fencing conditions were different from those under grazing conditions. Therefore, cold-season grazing can have lower negative effects on forage nutrient storages than warm-season grazing. Combined plant species with phylogenetic diversity and composition can be better in predicting forage nutrient storages. Grazing can restructure the elevation distribution of forage nutrient storages in alpine grasslands.

Physiology of Leymus chinensis under seasonal grazing: Implications for the development of sustainable grazing in a temperate grassland of Inner Mongolia.

Plants have different physiological characteristics as the season changes, grazing management in compliance with plant growth and development characteristics may provide new ideas for sustainable livestock development. However, there has been little research on seasonal grazing and plants physiological responses under it. Here, we studied a typical steppe ecosystem of Inner Mongolia, with Leymus chinensis as the dominant species, in five grazing treatments: continuous grazing, seasonal grazing (which started in spring or in early and late summer), and no grazing (the control). We analyzed growth and resistance of L. chinensis in the five treatments by measuring annual primary productivity, morphological traits and various physiological processes. Compared with continuous grazing, seasonal grazing significantly alleviated grassland degradation. The plants were less affected by stress under spring grazing, with net photosynthesis and non-photochemical quenching closer to the control values and with a lower malondialdehyde content. The annual primary production of plants under grazing started in the early and late summer were 3-4 times the value under continuous grazing. Regrowth under early-summer grazing was greatly improved, and stress resistance was stronger with a higher proline content and high antioxidant enzyme activity. And nutrient accumulation at the end of the growing season such as abundant soluble sugars were transferred from aboveground tissue to the roots in September under late-summer grazing, which benefited regrowth the next year. All these physiological processes were regulated by hormonal changes. Our results highlight how plants response grazing stress in different growing seasons and suggest that seasonal grazing can improve the stress resistance and regrowth capacity of forage vegetation, and applying this knowledge can promote more sustainable grazing practices.