ABSTRACT
La planta de uso medicinal Allophylus edulis se utiliza para el tratamiento de enfriamiento, catarro, diabetes y la planta Matricaria recutita (manzanilla) se utiliza como antiinflamatorio, descongestivo, tratar conjuntivitis, cólicos. Ambas especies son de amplio uso a nivel nacional, en Paraguay, e internacional, pero no se conocen bien sus posibles efectos secundarios sobre embriones de vertebrados. El objetivo de este trabajo fue evaluar si los extractos acuosos de, Allophylus edulis y Matricaria recutita tienen efecto tóxico en embriones de Danio rerio. Se trataron embriones de 6 horas pos fecundación con diferentes concentraciones de cada extracto para determinar la toxicidad de los extractos acuosos de Allophylus edulis y Matricaria recutita. En los resultados obtenidos, ambos extractos presentaron efectos tóxicos significativos en los siguientes parámetros: ausencia de formación de somitas, de latidos cardiacos, de circulación sanguínea y de pigmentación. No se observó un efecto teratogénico significativo. Se concluye que los extractos acuosos de Allophylus edulis y Matricaria recutita resultaron tóxicos para embriones de Danio rerio, de manera dosis dependiente. (AU)
Medicinal plant Allophylus edulis is used to treat colds, catarrh, diabetes and the Matricaria recutita plant (chamomile) is used as an anti-inflammatory, decongestant, conjunctivitis treatment, and colic. Both species are widely used nationally in Paraguay, and internationally, but their possible side effects on vertebrate embryos are not well known. The objectives of this work were to evaluate whether the aqueous extracts of Allophylus edulis and Matricaria recutita have a toxic effect on Danio rerio embryos. Embryos 6 hours after its fertilization were treated with different concentrations of each extract to determine the toxicity of the aqueous extracts of Allophylus edulis and Matricaria recutita. In the results obtained, both extracts presented significant toxic effects on the following parameters: absence of somite formation, heartbeat, blood circulation and pigmentation. No significant teratogenic effect was observed. It is concluded that the aqueous extracts of Allophylus edulis and Matricaria recutita were toxic for Danio rerio embryos, in a dose dependent way. (AU)
Subject(s)
Animals , Matricaria/poisoning , Plants, Medicinal/toxicity , Plant Extracts/toxicity , Zebrafish , Embryo, NonmammalianABSTRACT
Green synthesis by using biological agents has been a simple and effective approach for the synthesis of various forms of nanoparticles. The present investigation was intended to synthesis Ag-NPs and ZnO-NPs under photo-condition using the aqueous extracts of two mangrove plants namely Heritiera fomes and Sonneratia apetala and evaluate their potential biomedical applications. The formation of nanoparticles in aqueous solution of H. fomes and S. apetala under exposure to sun light was validated by change in color and formation of monodispersed NPs with a narrow particle size distribution. Fourier transform infrared spectroscopy (FT-IR) reveals the presence of Oxime and other heterocyclic compounds to be the most probable compounds responsible for the reduction and stability of nanoparticles in the solutions. The synthesized NPs displayed moderate free radical scavenging properties. The anti-inflammatory potential of ZnO-NPs was recorded to be comparatively higher than that of Ag-NP with 79% and 69.1% respectively. The Ag-NPs with unique properties of inhibiting α-amylase (91.14% and 89.16%) were found to be significantly high indicating its antidiabetic property. The synthesized NPs showed varied zone of inhibition (9-16mm) against the tested microbial pathogens. The synthesized nanoparticles possess strong biological activities in terms of antioxidant, anti-inflammatory, antidiabetic and antibacterial, potentials which could be utilized in various biological applications by the cosmetic, food and biomedical industries.
