Microencapsulation of phenolic compounds extracted from soybean seed coats by spray-drying.

This study aimed to characterize and microencapsulate soybean seed coats phenolic compounds by spray-drying, evaluating physicochemical properties and storage stability. Different extraction methodologies were used to obtain crude extract (SCE), ethyl acetate fraction, water fraction, and bound phenolic extract. Extraction yield, total phenolic and flavonoid contents, and antioxidant capacity were determined. HPLC-electrospray ionization source-MS/MS analysis was performed on SCE. Microencapsulation by spray-drying of SCE incorporating 10%, 20%, and 30% maltodextrin (MD) was carried out. Drying yield (DY), encapsulation efficiency (EE), moisture, morphology and particle size, dry, and aqueous storage stability were evaluated on the microcapsules. SCE had 7.79 g/100 g polyphenolic compounds (mainly isoflavones and phenolic acids) with antioxidant activity. Purification process by solvent partitioning allowed an increase of phenolic content and antioxidant activity. Microcapsules with 30% MD exhibited the highest DY, EE, and stability. Microencapsulated polyphenolic compounds from soybean seed coats can be used as functional ingredients in food products. PRACTICAL APPLICATION: Soybean seed coat is a usually discarded agro-industrial by-product, which presents antioxidant compounds of interest to human health. These compounds are prone to oxidation due to their chemical structure; therefore, microencapsulation is a viable and reproducible solution to overcome stability-related limitations. Microencapsulation of soybean seed coats polyphenols is an alternative which protects and extends the stability of phenolic compounds that could be potentially incorporated into food products as a natural additive with antioxidant properties.

Masks thermal degradation as an alternative of waste valorization on the COVID-19 pandemic: A kinetic study.

The COVID-19 pandemic generated a new dynamic around waste management. Personal protective equipment such as masks, gloves, and face shields were essential to prevent the spread of the disease. However, despite the increase in waste, no technical alternatives were foreseen for the recovery of these wastes, which are made up of materials that can be valued for energy recovery. It is essential to design processes such as waste to energy to promote the circular economy. Therefore, techniques such as pyrolysis and thermal oxidative decomposition of waste materials need to be studied and scaled up, for which kinetic models and thermodynamic parameters are required to allow the design of this reaction equipment. This work develops kinetic models of the thermal degradation process by pyrolysis as an alternative for energy recovery of used masks generated by the COVID-19 pandemic. The wasted masks were isolated for 72 h for virus inactivation and characterized by FTIR-ATR spectroscopy, elemental analysis, and determinate the higher calorific value (HCV). The composition of the wasted masks included polypropylene, polyethylene terephthalate, nylon, and spandex, with higher calorific values than traditional fuels. For this reason, they are susceptible to value as an energetic material. Thermal degradation was performed by thermogravimetric analysis at different heating rates in N2 atmosphere. The gases produced were characterized by gas chromatography and mass spectrometry. The kinetic model was based on the mass loss of the masks on the thermal degradation, then calculated activation energies, reaction orders, pre-exponential factors, and thermodynamic parameters. Kinetics models such as Coats and Redfern, Horowitz and Metzger, Kissinger-Akahira-Sunose were studied to find the best-fit models between the experimental and calculated data. The kinetic and thermodynamic parameters of the thermal degradation processes demonstrated the feasibility and high potential of recovery of these residues with conversions higher than 89.26% and obtaining long-chain branched hydrocarbons, cyclic hydrocarbons, and CO2 as products.

Retinopatía del prematuro con presentación atípica / Retinopathy of prematurity with atypical presentation

La retinopatía de la prematuridad es una enfermedad dinámica vasoproliferativa de la retina inmadura postnatal que afecta a los bebés prematuros. Cuando aparecen signos de atipicidad en su diagnóstico o evolución deben descartarse otras entidades vasculares de la retina, que generalmente tienen un trasfondo genético y semejan o coexisten con dicha entidad. Se presenta un caso con características de Retinopatía del prematuro y algunos signos de atipicidad. Se describe su manejo y evolución, así como una breve descripción de las entidades que conforman el diagnóstico diferencial(AU)

Retinopathy of prematurity is a dynamic vasoproliferative disease of the immature postnatal retina that affects premature babies. When signs of atypicality appear in its diagnosis or evolution, other vascular entities of the retina must be ruled out, which generally have a genetic background and resemble or coexist with said entity. We present a case with characteristics of Retinopathy of prematurity and some signs of atypicality. Its management and evolution are described, as well as a brief description of the entities that make up the differential diagnosis(AU)

A patient with X-linked retinoschisis and exudative retinal detachment associated with a pathogenic hemizygous variant c.304c>T in RS1.

BACKGROUND: X-linked retinoschisis (XLRS) is a rare retinal dystrophy due to pathogenic variants in the RS1 gene. The hallmark of the disease is a foveal spoke-wheel appearance. The purpose of this report is to expand the phenotypic spectrum of XLRS reporting a patient with atypical phenotype of XLRS associated with Coats-like phenotype. MATERIALS AND METHODS: This is a case report of a patient diagnosed with XLRS who underwent ophthalmologic multimodal imaging and next-generation sequencing panel. RESULTS: The proband is a 14-year-old male patient who presented at Instituto Suel Abujamra with a history of Coats Disease in the right eye treated with retinal laser in both eyes two years ago. His best-corrected visual acuity was count finger at 1 foot in the right eye and 20/40 in the left eye. Fundus exam showed an extensive area of exudation and retinal detachment in the right eye and cystic change at the fovea in a spoke-wheel pattern in the left eye. The next-generation sequencing panel targeting inherited retinal diseases with 236 genes found a pathogenic hemizygous variant c.304C>T (p.Arg102Trp) in RS1 that has already been reported. CONCLUSIONS: The association of peripheral vascular incompetence and XLRS has already been described. Retinal exudation in the setting of XLRS is probably the result of vascular disruption and compromise. The loss of retinoschisin function that leads to foveal retinoschisis may also lead to vascular anomalies.

Primary Ovarian Failure in Addition to Classical Clinical Features of Coats Plus Syndrome in a Female Carrying 2 Truncating Variants of CTC1.

Coats plus syndrome is an autosomal recessive multisystemic and pleiotropic disorder affecting the eyes, brain, bone, and gastrointestinal tract, usually caused by compound heterozygous variants of the conserved telomere maintenance component 1 gene (CTC1), involved in telomere homeostasis and replication. So far, most reported patients are compound heterozygous for a truncating mutation and a missense variant. The phenotype is believed to result from telomere dysfunction, with accumulation of DNA damage, cellular senescence, and stem cell depletion. Here, we report a 23-year-old female with prenatal and postnatal growth retardation, microcephaly, osteopenia, recurrent fractures, intracranial calcification, leukodystrophy, parenchymal brain cysts, bicuspid aortic valve, and primary ovarian failure. She carries a previously reported maternally inherited pathogenic variant in exon 5 (c.724_727del, p.(Lys242Leufs*41)) and a novel, paternally inherited splice site variant (c.1617+5G>T; p.(Lys480Asnfs*17)) in intron 9. CTC1 transcript analysis showed that the latter resulted in skipping of exon 9. A trace of transcripts was normally spliced resulting in the presence of a low level of wild-type CTC1 transcripts. We speculate that ovarian failure is caused by telomere shortening or chromosome cohesion failure in oocytes and granulosa cells, with early decrease in follicular reserve. This is the first patient carrying 2 truncating CTC1 variants and the first presenting primary ovarian failure.

Gastrointestinal Hemorrhage: A Manifestation of the Telomere Biology Disorders.

OBJECTIVE: To describe the clinical features, therapeutic interventions, and patient outcomes of gastrointestinal (GI) hemorrhage in individuals with a telomere biology disorder, including dyskeratosis congenita, Hoyeraal-Hreidarsson syndrome, Revesz syndrome, and Coats plus. STUDY DESIGN: Clinical Care Consortium for Telomere Associated Ailments members were invited to contribute data on individuals with telomere biology disorders at their institutions who experienced GI bleeding. Patient demographic, laboratory, imaging, procedural, and treatment information and outcomes were extracted from the medical record. RESULTS: Sixteen patients who experienced GI hemorrhage were identified at 11 centers. Among 14 patients who underwent genetic testing, 8 had mutations in TINF2, 4 had mutations in CTC1 or STN1, and 1 patient each had a mutation in TERC and RTEL1. Ten patients had a history of hematopoietic cell transplantation. The patients with Coats plus and those without Coats plus had similar clinical features and courses. Angiodysplasia of the stomach and/or small bowel was described in 8 of the 12 patients who underwent endoscopy; only 4 had esophageal varices. Various medical interventions were trialed. No single intervention was uniformly associated with cessation of bleeding, although 1 patient had a sustained response to treatment with bevacizumab. Recurrence was common, and the overall long-term outcome for affected patients was poor. CONCLUSIONS: GI bleeding in patients with telomere biology disorders is associated with significant morbidity and with vascular ectasias rather than varices.

Assessment of the use of cooked Araucaria angustifolia seed coats extract as food for brine shrimp.

The use of vegetable waste and its screening for potential cytotoxicity is of utmost importance to ensure its safe use in the feed industry for fish and other animals. We evaluated aqueous and ethanolic extracts of cooked Araucaria angustifolia seed coats. The Stiasny index for the aqueous and ethanolic extracts was 2.87% ± 0.03% and 60.53% ± 4.79%, respectively. Condensed tannins were 11-fold higher in the ethanolic extract than the aqueous extract. The flavonoid and polyphenol contents were 1.7- and 1.8-fold higher in the ethanolic extract than in the aqueous extract, respectively. The 36 h EC50 for brine shrimp hatchability was 300.32 µg/mL for the aqueous extract, and 76.60 µg/mL for the ethanolic extract. The 24 h LC50 was 1405.96 µg/mL for the aqueous extract, and it was 356.32 µg/mL for the ethanolic extract. The aqueous extract was nontoxic to A. salina nauplii, and therefore, it can be used as a possible food additive in fish feed. The results also demonstrated that the different solvents used in the extraction affected the yield and the total phenolic, total flavonoid, and condensed tannin content. Further in vivo and cell line cytotoxicity testing is recommended to substantiate these findings.

Enfermedad de coats / Coats disease

RESUMEN Introducción: La enfermedad de Coats es una vasculopatía retiniana idiopática, poco frecuente, que puede progresar a desprendimiento de retina exudativo y glaucoma neovascular. Típicamente se presenta en la infancia, es la tercera causa más frecuente de leucocoria infantil. Presentación de caso: Paciente masculino de dos años de edad, procedente de zona rural. Asiste a consulta, porque la mama notó que el niño desvía el ojo derecho hacia fuera desde que nació. Conclusiones: La enfermedad de Coats simula otras vasculopatías retinianas y el retinoblastoma. La conducta a seguir dependerá de la, forma clínica de presentación y complicaciones asociadas.

ABSTRACT Introduction: Coats disease is a rare, idiopathic retinal vasculopathy that can progress to exudative retinal detachment and neovascular glaucoma. Typically occurring in childhood, it is the third most common cause of childhood leukocoria. Case report: Patient masculine of two years of age, coming from rural area. You attended consultation, because he/she suckles her he/she noticed that the boy deviates the right eye toward it was since he was born. Conclusions: Coats disease simulates other retinal vasculopathies and retinoblastoma. The behavior to be followed will depend on the clinical form of presentation and associated complications.

Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students.

This study aimed at detecting Staphylococcus aureus from white coats of college students and characterizing antimicrobial susceptibility and biofilm production. Bacterial samples (n = 300) were obtained from white coats of 100 college students from August 2015 to March 2017 S. aureus was isolated and it´s resistance profile was assessed by antimicrobial disk-diffusion technique, screening for methicillin-resistant Staphylococcus aureus (MRSA), detection of mecA gene by PCR, and determination of staphylococcal cassette chromosome mec (SCCmec) by multiplex PCR. Congo red agar (CRA) and icaA and icaD genes by PCR were used for biofilm characterization. S. aureus was identified in 45.0% of samples. Resistance of S. aureus sample to antimicrobial was seen for penicillin (72.59%), erythromycin (51.85%), cefoxitin (20.74%), oxacillin (17.04%), clindamycin (14.81%) and levofloxacin (5.18%). MRSA was detected in 53.3% of the samples with SCCmec I (52.8%), SCCmec III (25%) and SCCmec IV (11.1%). Biofilm production was observed in 94.0% S. aureus samples. These data show that biosafety measures need to be enhanced in order to prevent dissemination of multiresistant and highly adhesive bacteria across other university settings, relatives, and close persons.

Black bean anthocyanin-rich extracts as food colorants: Physicochemical stability and antidiabetes potential.

Black beans contain anthocyanins that could be used as colorants in foods with associated health benefits. The objective was to optimize anthocyanins extraction from black bean coats and evaluate their physicochemical stability and antidiabetes potential. Optimal extraction conditions were 24% ethanol, 1:40 solid-to-liquid ratio and 29°C (P<0.0001). Three anthocyanins were identified by MS ions, delphinidin-3-O-glucoside (465.1m/z), petunidin-3-O-glucoside (479.1m/z) and malvidin-3-O-glucoside (493.1m/z). A total of 32mg of anthocyanins were quantified per gram of dry extract. Bean anthocyanins were stable at pH 2.5 and low-temperature 4°C (89.6%), with an extrapolated half-life of 277days. Anthocyanin-rich extracts inhibited α-glucosidase (37.8%), α-amylase (35.6%), dipeptidyl peptidase-IV (34.4%), reactive oxygen species (81.6%), and decreased glucose uptake. Black bean coats are a good source of anthocyanins and other phenolics with the potential to be used as natural-source food colorants with exceptional antidiabetes potential.

Expression of genes SBP and leginsulin in contrasting soybean seed coats / Expressão dos genes SBP e leginsulin em tegumentos de sementes de soja contrastantes

Evaluation of differential candidate gene expression in contrasting soybean seeds is an auxiliary tool in the partial elucidation of processes involved in seeds formation, as well as it contributes to the generation of new information that can be used in future research or in the development of r genetic superior constitutions. The aim of this study was to evaluate the expression of two candidate genes, SBP and leginsulin genes, possibly involved in seed quality, in contrasting coats of four soybean genotypes. Two cultivars of yellow soybeans were used, BMX Potência RR and CD 202, and two lines of black soybean, TP and IAC. Gene expression was evaluated using qPCR in seven stages of development from seed coats for four genotypes, at 25, 30, 35, 40, 45, 50, and 55 days after anthesis. The design was completely randomized, with three replications. Data were subjected to analysis of variance and means compared by Tukey's test at 5% probability. SBP and leginsulin gene have higher expression in the early phases of development from seed coats of BMX Potência RR cultivar, followed by the IAC line. These genotypes are therefore of interest for further research involving these genes.(AU)

A avaliação da expressão diferencial de genes candidatos em sementes de soja contrastantes é uma ferramentaauxiliar na elucidação parcial dos processos envolvidos na formação de sementes, além de contribuir para a geração de novas informações que possam ser usadas em futuras pesquisas ou para o desenvolvimento de constituições genéticas superiores. O objetivo do trabalho foi avaliar a expressão de dois genes candidatos, SBP e leginsulin, possivelmente envolvidos com a qualidade das sementes, em tegumentos contrastantes de quatro genótipos de soja. Foram utilizadas duas cultivares de tegumentos amarelos, BMX Potência RR e CD 202, e duas linhagens de tegumentos pretos, TP e IAC. A expressão dos genes foi avaliada pela técnica qPCR, em sete fases de desenvolvimento dos tegumentos para os quatro genótipos, aos 25, 30, 35, 40, 45, 50 e 55 dias após a antese. O delineamento foi o inteiramente casualizado, com três repetições. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey, em nível de 5% de probabilidade. Os genes SBP e leginsulinapresentam maior expressão nas fases iniciais de desenvolvimento dos tegumentos de BMX Potência RR, seguido de IAC, sendo estes genótipos de interesse para novas pesquisas envolvendo estes genes.(AU)

Expression of genes SBP and leginsulin in contrasting soybean seed coats / Expressão dos genes SBP e leginsulin em tegumentos de sementes de soja contrastantes

ABSTRACT: Evaluation of differential candidate gene expression in contrasting soybean seeds is an auxiliary tool in the partial elucidation of processes involved in seeds formation, as well as it contributes to the generation of new information that can be used in future research or in the development of r genetic superior constitutions. The aim of this study was to evaluate the expression of two candidate genes, SBP and leginsulin genes, possibly involved in seed quality, in contrasting coats of four soybean genotypes. Two cultivars of yellow soybeans were used, BMX Potência RR and CD 202, and two lines of black soybean, TP and IAC. Gene expression was evaluated using qPCR in seven stages of development from seed coats for four genotypes, at 25, 30, 35, 40, 45, 50, and 55 days after anthesis. The design was completely randomized, with three replications. Data were subjected to analysis of variance and means compared by Tukey's test at 5% probability. SBP and leginsulin gene have higher expression in the early phases of development from seed coats of BMX Potência RR cultivar, followed by the IAC line. These genotypes are therefore of interest for further research involving these genes.

RESUMO: A avaliação da expressão diferencial de genes candidatos em sementes de soja contrastantes é uma ferramentaauxiliar na elucidação parcial dos processos envolvidos na formação de sementes, além de contribuir para a geração de novas informações que possam ser usadas em futuras pesquisas ou para o desenvolvimento de constituições genéticas superiores. O objetivo do trabalho foi avaliar a expressão de dois genes candidatos, SBP e leginsulin, possivelmente envolvidos com a qualidade das sementes, em tegumentos contrastantes de quatro genótipos de soja. Foram utilizadas duas cultivares de tegumentos amarelos, BMX Potência RR e CD 202, e duas linhagens de tegumentos pretos, TP e IAC. A expressão dos genes foi avaliada pela técnica qPCR, em sete fases de desenvolvimento dos tegumentos para os quatro genótipos, aos 25, 30, 35, 40, 45, 50 e 55 dias após a antese. O delineamento foi o inteiramente casualizado, com três repetições. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey, em nível de 5% de probabilidade. Os genes SBP e leginsulinapresentam maior expressão nas fases iniciais de desenvolvimento dos tegumentos de BMX Potência RR, seguido de IAC, sendo estes genótipos de interesse para novas pesquisas envolvendo estes genes.

Enfermedad de Coats, presentación de un caso y revisión de la literatura / Coats Disease, case report and literatura review

La enfermedad de Coats, es una vasculopatía retiniana idiopática que sin tratamiento puede progresar a desprendimiento de retina exudativo y glaucoma neovascular. Se considera una patología de difícil diagnóstico, por su presentación clínica que simula otras vasculopatías retinianas ó retinoblastoma. Resulta indispensable contar con herramientas clínicas y paraclínicas para llegar a un diagnóstico acertado y poder realizar un manejo adecuado y oportuno. Presentamos el caso de un niño de 17 años, con enfermedad de Coats valorado y tratado en el Hospital Militar Central de Bogotá desde los 5 años de edad; Se realiza seguimiento clínico de 12 años de evolución y se discuten posibles diagnósticos diferenciales.

Coats disease is an idiopathic retinal vasculopathy that can progress to exudative retinal detachment and neovascular glaucoma if is not treated. It is considered a condition of difficult diagnosis due to clinical presentation, simulating retinoblastoma or other retinal vascular disease. It is essential to have clinical and paraclinical tools to have a correct diagnosis and to make adequate and timely handling. We report the case of a child of 17 years old with Coats disease assessed and treated at the Central Military Hospital in Bogota from 5 years of age during 12 years. Th e clinical evolution is described and a brief depiction of the disease is presented with its diff erential diagnoses.

Coat colour's studies in relation to the productive variables of crossbred sheep under heat stress / Estudos da coloração do pelame em relação às respostas produtivas de ovinos mestiços sob estresse calórico

The aim of this research was to evaluate the yield responses of crossbred sheep Santa Ines with Dorper, with an average age of four months, 15 of dark coat and 15 of white coat, under heat stress. The animals were exposed to solar radiation for one hour per week (14.00 - 15.00) for eight weeks. Measurements of height of the dorsum, rump height, chest width, rump width, thoracic perimeter, thigh perimeter, hind perimeter and scrotal perimeter were made. The animals were slaughtered and were weighed the following components: blood, skin, liver, heart, kidneys, head, legs, lungs, testicles and penis / urethra. The gastrointestinal tract was weighed full and empty. The hot carcass weight and cold carcass weight and calculated cooling losses and hot carcass yield and cold were evaluated; beyond of the commercial cuts: palette, leg, neck, loin and rib. Finishing and carcass conformation and color assessment, marbling, texture and subcutaneous fat thickness were also evaluated. Black sheeps had heavier kidneys than the white sheeps. Testicles, the hot carcass, palette, leg, rib and rump width of black sheeps were lighter towards animals white fur. Heat stress can cause disturbances in the metabolism of sheep, affecting their body condition and altering its development.(AU)

Objetivou-se avaliar as respostas produtivas de 30 ovinos mestiços ½Santa Inês + ½Dorper, com idade média de quatro meses, 15 com pelame preto e 15 com pelame branco, submetidos a estresse térmico. Os animais sofreram exposição à radiação solar direta durante uma hora (14h00 15h00) por semana durante oito semanas. Foram feitas as medições de altura do dorso, altura da garupa, largura do tórax, largura da garupa, perímetro do tórax, perímetro da coxa, perímetro da garupa e perímetro escrotal. Os animais foram abatidos e houve a pesagem dos seguintes componentes: sangue, pele, fígado, coração, rins, cabeça, patas, pulmão, trato gastrointestinal e vazio, testículos e pênis/uretra. Foram avaliados os pesos de carcaça quente e carcaça fria e calculadas as perdas por resfriamento e rendimento de carcaça quente e carcaça fria, além dos cortes comerciais: paleta, perna, pescoço, lombo e costela. Também foram avaliados o acabamento e conformação da carcaça e avaliação da cor, marmoreio, textura e espessura de gordura subcutânea. Os ovinos pretos apresentaram maior peso de rins e menor peso de testículos, carcaça quente, paleta, perna, costela e menor medida de largura da garupa em relação aos animais de pelame branco. O estresse térmico pode provocar distúrbios no metabolismo dos ovinos, afetando sua condição corporal e alterando seu desenvolvimento.(AU)

Coat colour's studies in relation to the productive variables of crossbred sheep under heat stress / Estudos da coloração do pelame em relação às respostas produtivas de ovinos mestiços sob estresse calórico

The aim of this research was to evaluate the yield responses of crossbred sheep Santa Ines with Dorper, with an average age of four months, 15 of dark coat and 15 of white coat, under heat stress. The animals were exposed to solar radiation for one hour per week (14.00 - 15.00) for eight weeks. Measurements of height of the dorsum, rump height, chest width, rump width, thoracic perimeter, thigh perimeter, hind perimeter and scrotal perimeter were made. The animals were slaughtered and were weighed the following components: blood, skin, liver, heart, kidneys, head, legs, lungs, testicles and penis / urethra. The gastrointestinal tract was weighed full and empty. The hot carcass weight and cold carcass weight and calculated cooling losses and hot carcass yield and cold were evaluated; beyond of the commercial cuts: palette, leg, neck, loin and rib. Finishing and carcass conformation and color assessment, marbling, texture and subcutaneous fat thickness were also evaluated. Black sheeps had heavier kidneys than the white sheeps. Testicles, the hot carcass, palette, leg, rib and rump width of black sheeps were lighter towards animals white fur. Heat stress can cause disturbances in the metabolism of sheep, affecting their body condition and altering its development.

Objetivou-se avaliar as respostas produtivas de 30 ovinos mestiços ½Santa Inês + ½Dorper, com idade média de quatro meses, 15 com pelame preto e 15 com pelame branco, submetidos a estresse térmico. Os animais sofreram exposição à radiação solar direta durante uma hora (14h00 15h00) por semana durante oito semanas. Foram feitas as medições de altura do dorso, altura da garupa, largura do tórax, largura da garupa, perímetro do tórax, perímetro da coxa, perímetro da garupa e perímetro escrotal. Os animais foram abatidos e houve a pesagem dos seguintes componentes: sangue, pele, fígado, coração, rins, cabeça, patas, pulmão, trato gastrointestinal e vazio, testículos e pênis/uretra. Foram avaliados os pesos de carcaça quente e carcaça fria e calculadas as perdas por resfriamento e rendimento de carcaça quente e carcaça fria, além dos cortes comerciais: paleta, perna, pescoço, lombo e costela. Também foram avaliados o acabamento e conformação da carcaça e avaliação da cor, marmoreio, textura e espessura de gordura subcutânea. Os ovinos pretos apresentaram maior peso de rins e menor peso de testículos, carcaça quente, paleta, perna, costela e menor medida de largura da garupa em relação aos animais de pelame branco. O estresse térmico pode provocar distúrbios no metabolismo dos ovinos, afetando sua condição corporal e alterando seu desenvolvimento.

Estudos da coloração do pelame em relação às respostas produtivas de ovinos mestiços sob estresse calórico

The aim of this research was to evaluate the yield responses of crossbred sheep Santa Ines with Dorper, with an average age of four months, 15 of dark coat and 15 of white coat, under heat stress. The animals were exposed to solar radiation for one hour per week (14.00 - 15.00) for eight weeks. Measurements of height of the dorsum, rump height, chest width, rump width, thoracic perimeter, thigh perimeter, hind perimeter and scrotal perimeter were made. The animals were slaughtered and were weighed the following components: blood, skin, liver, heart, kidneys, head, legs, lungs, testicles and penis / urethra. The gastrointestinal tract was weighed full and empty. The hot carcass weight and cold carcass weight and calculated cooling losses and hot carcass yield and cold were evaluated; beyond of the commercial cuts: palette, leg, neck, loin and rib. Finishing and carcass conformation and color assessment, marbling, texture and subcutaneous fat thickness were also evaluated. Black sheeps had heavier kidneys than the white sheeps. Testicles, the hot carcass, palette, leg, rib and rump width of black sheeps were lighter towards animals white fur. Heat stress can cause disturbances in the metabolism of sheep, affecting their body condition and altering its development.

Objetivou-se avaliar as respostas produtivas de 30 ovinos mestiços ½Santa Inês + ½Dorper, com idade média de quatro meses, 15 com pelame preto e 15 com pelame branco, submetidos a estresse térmico. Os animais sofreram exposição à radiação solar direta durante uma hora (14h00 15h00) por semana durante oito semanas. Foram feitas as medições de altura do dorso, altura da garupa, largura do tórax, largura da garupa, perímetro do tórax, perímetro da coxa, perímetro da garupa e perímetro escrotal. Os animais foram abatidos e houve a pesagem dos seguintes componentes: sangue, pele, fígado, coração, rins, cabeça, patas, pulmão, trato gastrointestinal e vazio, testículos e pênis/uretra. Foram avaliados os pesos de carcaça quente e carcaça fria e calculadas as perdas por resfriamento e rendimento de carcaça quente e carcaça fria, além dos cortes comerciais: paleta, perna, pescoço, lombo e costela. Também foram avaliados o acabamento e conformação da carcaça e avaliação da cor, marmoreio, textura e espessura de gordura subcutânea. Os ovinos pretos apresentaram maior peso de rins e menor peso de testículos, carcaça quente, paleta, perna, costela e menor medida de largura da garupa em relação aos animais de pelame branco. O estresse térmico pode provocar distúrbios no metabolismo dos ovinos, afetando sua condição corporal e alterando seu desenvolvimento.

Estudos da coloração do pelame em relação às respostas produtivas de ovinos mestiços sob estresse calórico

The aim of this research was to evaluate the yield responses of crossbred sheep Santa Ines with Dorper, with an average age of four months, 15 of dark coat and 15 of white coat, under heat stress. The animals were exposed to solar radiation for one hour per week (14.00 - 15.00) for eight weeks. Measurements of height of the dorsum, rump height, chest width, rump width, thoracic perimeter, thigh perimeter, hind perimeter and scrotal perimeter were made. The animals were slaughtered and were weighed the following components: blood, skin, liver, heart, kidneys, head, legs, lungs, testicles and penis / urethra. The gastrointestinal tract was weighed full and empty. The hot carcass weight and cold carcass weight and calculated cooling losses and hot carcass yield and cold were evaluated; beyond of the commercial cuts: palette, leg, neck, loin and rib. Finishing and carcass conformation and color assessment, marbling, texture and subcutaneous fat thickness were also evaluated. Black sheeps had heavier kidneys than the white sheeps. Testicles, the hot carcass, palette, leg, rib and rump width of black sheeps were lighter towards animals white fur. Heat stress can cause disturbances in the metabolism of sheep, affecting their body condition and altering its development.

Objetivou-se avaliar as respostas produtivas de 30 ovinos mestiços ½Santa Inês + ½Dorper, com idade média de quatro meses, 15 com pelame preto e 15 com pelame branco, submetidos a estresse térmico. Os animais sofreram exposição à radiação solar direta durante uma hora (14h00 15h00) por semana durante oito semanas. Foram feitas as medições de altura do dorso, altura da garupa, largura do tórax, largura da garupa, perímetro do tórax, perímetro da coxa, perímetro da garupa e perímetro escrotal. Os animais foram abatidos e houve a pesagem dos seguintes componentes: sangue, pele, fígado, coração, rins, cabeça, patas, pulmão, trato gastrointestinal e vazio, testículos e pênis/uretra. Foram avaliados os pesos de carcaça quente e carcaça fria e calculadas as perdas por resfriamento e rendimento de carcaça quente e carcaça fria, além dos cortes comerciais: paleta, perna, pescoço, lombo e costela. Também foram avaliados o acabamento e conformação da carcaça e avaliação da cor, marmoreio, textura e espessura de gordura subcutânea. Os ovinos pretos apresentaram maior peso de rins e menor peso de testículos, carcaça quente, paleta, perna, costela e menor medida de largura da garupa em relação aos animais de pelame branco. O estresse térmico pode provocar distúrbios no metabolismo dos ovinos, afetando sua condição corporal e alterando seu desenvolvimento.

Microarchitectural adaptations in the stomach of African tree pangolin (Manis tricuspis) / Adaptaciones microarquitecturales en el estómago del pangolin africano (Manis tricuspis)

The microarchitecture of the pangolin's stomach favouring the high chitinous diet has been less waived into, despite extensive morphological investigations. Histological analysis of the microanatomy will provide powerful tools for interpretation to yield reliable insights. We investigated this by fixing the tissues in 10 percent formol saline for histological analysis. Serial sections at 5 micron m thickness were subjected to general staining methods for light microscopic study (Haematoxylin and eosin, Van Gieson's and Verhoeff's). The results revealed basic structural arrangements in their coats, with a modification of the epithelial lining of cardia and fundus into stratified squamous keratinized epithelium. These modifications were also reflected in the distribution of collagen and elastic fibers in the various layers (coats) of the stomach. The present study has shown that there was an adaptation of the stomach of African tree pangolin to its diet as reflected in the microarchitectural configuration.

La micro arquitectura del estómago de los pangolines que favorece la alta dieta de chitinous sido poco tomada en cuenta, a pesar de las amplias investigaciones morfológicas. El análisis histológico de la microanatomía proporcionará herramientas de gran importancia para la interpretación, junto con dar una información confiable. Se investigó mediante la fijación de los tejidos en solución salina de formol al 10 por ciento para análisis histológico. Las serie de secciones fueron sometidos a métodos de tinción estándar para el estudio con microscopía de luz (hematoxilina y eosina, Van Gieson y Verhoeff s). Los resultados revelaron adaptaciones estructurales básicas en sus capas, con una modificación del revestimiento epitelial del cardias y fundus en epitelio escamoso estratificado (queratinizado). Estas modificaciones también se reflejan en la distribución de colágeno y fibras elásticas en las diversas capas del estómago. El presente estudio ha demostrado que es una adaptación del estómago a la dieta como se refleja en la configuración de la microarquitectura.