RESUMEN
A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR studies. Examples of one nutrient uptake and one multidrug extrusion protein from Helicobacter pylori are described. This strategy is successful for membrane proteins from H. pylori, E. coli, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microbacterium liquefaciens, Brucella abortus, Brucella melitensis, Campylobacter jejuni, Neisseria meningitides, Streptomyces coelicolor and Rhodobacter sphaeroides.
Asunto(s)
Proteínas de Transporte de Membrana/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Transporte Biológico Activo , Dicroismo Circular , ADN Bacteriano/genética , Helicobacter pylori/química , Helicobacter pylori/genética , Helicobacter pylori/metabolismo , Proteínas de Transporte de Membrana/química , Proteínas de Transporte de Membrana/genética , Modelos Biológicos , Plásmidos , Mapeo Restrictivo , Simportadores/genética , Simportadores/metabolismoRESUMEN
Arginine anaerobic catabolism occurs in Bacillus licheniformis through the arginine deiminase pathway, encoded by the gene cluster arcABDC. We report here the involvement of a new protein, ArcR, in the regulation of the pathway. ArcR is a protein of the Crp/Fnr family encoded by a gene located 109 bp downstream from arcC. It binds to a palindromic sequence, very similar to an Escherichia coli Crp binding site, located upstream from arcA. Residues in the C-terminal domain of Crp that form the DNA binding motif, in particular residues Arg-180 and Glu-181 that make specific bonds with DNA, are conserved in ArcR, suggesting that the complexes formed with DNA by Crp and ArcR are similar. Moreover, the pattern of DNase I hypersensitivity sites induced by the binding of ArcR suggests that ArcR bends the DNA in the same way as Crp. From the absence of anaerobic induction following inactivation of arcR and from the existence of a binding site upstream of the arcA transcription start point, it can be inferred that ArcR is an activator of the arginine deiminase pathway.
Asunto(s)
Arginina/metabolismo , Bacillus/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Proteínas de Escherichia coli , Regulación Bacteriana de la Expresión Génica , Secuencia de Aminoácidos , Anaerobiosis , Bacillus/metabolismo , Proteínas Bacterianas/química , Secuencia de Bases , Sitios de Unión , Proteínas Portadoras , Proteína Receptora de AMP Cíclico/genética , Proteína Receptora de AMP Cíclico/metabolismo , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Proteínas de Unión al ADN/química , Genes Bacterianos , Proteínas Hierro-Azufre/genética , Proteínas Hierro-Azufre/metabolismo , Datos de Secuencia Molecular , Familia de Multigenes , Mutación , Análisis de Secuencia de ADNRESUMEN
The major transcription factors controlling arginine metabolism in Escherichia coli and Bacillus subtilis, ArgR and AhrC, respectively, are homologous multimeric proteins that form l -arginine-dependent DNA-binding complexes capable of repressing transcription of the biosynthetic genes (both), activating transcription of catabolic genes (AhrC only) or facilitating plasmid dimer resolution (both). Multimerisation and l -arginine binding are associated with the C-terminal 70-80 residues; the N-terminal regions contain a winged helix-turn-helix DNA-binding domain. We have constructed chimeric genes in which the sequences for the N and C-terminal domains have been swapped. The resultant chimeric proteins and their corresponding native proteins have been analysed for their ability to multimerise and bind DNA operator sites in an L-arginine-dependent fashion. Gel filtration and equilibrium sedimentation analysis are consistent with the formation of hexamers by all four proteins in the presence of L-arginine and at high protein concentrations (>100 nM monomer). The hexamer sedimentation coefficients suggest that there is a reduction in molecular volume upon binding L-arginine, consistent with a conformational change accompanying an allosteric activation of DNA-binding. In the absence of L-arginine or at lower protein concentrations, the hexamers are clearly in rapid equilibrium with smaller subunits, whose dominant species appear to be based on trimers, as expected from the crystal structure of the ArgR C-terminal fragment, with the exception of the ArgR-C chimera, which apparently dissociates into dimers, suggesting that in the intact protein the DNA-binding domains may have a significant dimeric interaction. The hexamer-trimer Kdis in the micromolar range, suggesting that trimers are the principal species at in vivo concentrations.DNA binding by all four proteins has been probed by gel retardation and DNase I footprinting analysis using all three types of naturally occurring operators: biosynthetic sites encompassing two 18 bp ARG boxes separated by 2 bp; biosynthetic sites containing two such boxes and a third 18 bp ARG box at a distance of 100 bp downstream, i.e. within the structural gene; and finally a catabolic operator which contains a single ARG box site. The data show that all four proteins bind to the operators at the expected regions in an L-arginine-dependent fashion. From the apparent affinities of the chimeras for each target site, there is no obvious sequence-specificity associated with the N-terminal domains; rather the data can be interpreted in terms of differential allosteric activation, including DNA binding in the absence of L-arginine.Remarkably, the proteins show apparent "anti-competition" in the presence of excess, specific DNA fragments in gel retardation. This appears to be due to assembly of an activated form of the protein, probably hexamers, on the operator DNA. The data are discussed in terms of the current models for the mode of action of both native proteins.
Asunto(s)
Arginina/metabolismo , Bacillus subtilis , Proteínas Bacterianas/metabolismo , Proteínas de Escherichia coli , Escherichia coli , Regiones Operadoras Genéticas , Proteínas Represoras/metabolismo , Transactivadores/metabolismo , Secuencia de Aminoácidos , Fusión Artificial Génica , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Secuencia de Bases , Huella de ADN , ADN Bacteriano , Desoxirribonucleasa I , Electroforesis en Gel de Poliacrilamida , Expresión Génica , Datos de Secuencia Molecular , Peso Molecular , Células Procariotas , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/aislamiento & purificación , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Represoras/genética , Proteínas Represoras/aislamiento & purificación , Transactivadores/genética , Transactivadores/aislamiento & purificación , UltracentrifugaciónRESUMEN
The commercial surface plasmon resonance (SPR) biosensors, BIACORE, have been used to investigate the molecular details of macromolecular interactions at prokaryotic promoter-operators. For the Escherichia coli methionine repressor, MetJ, we have quantitated the interaction of the protein with synthetic and natural operator sites and shown that the SPR response is directly related to the stoichiometry of the complexes being formed. The utility of a continuous flow system has also been exploited to investigate transcription from an immobilised promoter-operator fragment; with transcripts collected and subsequently characterised by RT-PCR. This technique has enabled us to investigate how repressor binding affects (i) the interaction of the RNA polymerase (RNAP) with the promoter and (ii) the ability of RNAP to initiate transcription. Remarkably, the repression complex appears to stabilise binding of RNAP, whilst having the expected effects on the levels of transcripts produced. This may well be a general mechanism allowing rapid transcription initiation to occur as soon as the repression complex dissociates. These techniques have also been used to examine protein-DNA interactions in the E. coli and Bacillus subtilis arginine repressor systems. The repressors are the products of the argR and ahrC genes, respectively. Both proteins form hexamers in rapid equilibrium with smaller subunits believed to be trimers. There are three types of operator in these systems, autoregulatory, biosynthetic and catabolic (B. subtilis only). Sensorgrams show that each protein recognises the three types of immobilised operator differently and that binding is stimulated over 100-fold by the presence of L-arginine.
Asunto(s)
Proteínas Bacterianas/análisis , Proteínas de Escherichia coli , Proteínas Represoras/análisis , Resonancia por Plasmón de Superficie , Transcripción Genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Escherichia coli/genética , Escherichia coli/metabolismo , Sustancias Macromoleculares , Datos de Secuencia Molecular , Proteínas Represoras/genética , Proteínas Represoras/metabolismoRESUMEN
Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistance. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin- producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.
Asunto(s)
Microbiología del Suelo , Streptomyces/aislamiento & purificación , Estreptomicina/biosíntesis , Secuencia de Aminoácidos , Técnicas de Tipificación Bacteriana , Secuencia de Bases , Brasil , ADN Bacteriano/análisis , Datos de Secuencia Molecular , Streptomyces/clasificación , Streptomyces/metabolismoRESUMEN
We have previously reported the initial characterization of a catabolic operator site (O[rocA]) for the Bacillus subtilis arginine repressor/activator protein AhrC. Here, we present the characterization by gel retardation and DNase I footprinting of both O(rocA) and a second catabolic operator site, O(rocD). Both operator sites encompass a single recognition site, an ARG box, located immediately upstream of the transcriptional start points, a unique positioning for a transcriptional activator protein. Although there is considerable sequence homology between the two catabolic operator sites, they vary significantly, around twofold, in their apparent affinities for the protein (K'd approximately 90 nM for O[rocA] and approximtaely 190nM for O[rocD]). This difference may result from the lower match to the ARG box consensus of the O(rocD) site. Both catabolic operators show evidence for co-operative binding with respect to protein concentration. Determination of the sequences of two AhrC catabolic operator sites, in combination with the three such biosynthetic sites, has allowed the derivation of an improved B. subtilis ARG box consensus sequence, CATGAATAAAAATg/tCAAg/t. This is not identical to the Escherichia coli consensus operator for the AhrC homologue, ArgR, which may explain the only partial cross-functioning of these proteins in vivo. The O(rocA) site is adjacent to a sharp, stable bend located 5' to the catabolic operator. Circular permutation analysis has been used to determine the relative angle of bend (approximately 50 degrees), its location and the effect of adding magnesium ions and/or AhrC protein. Protein binding increases the relative bend angle to approximately 85 degrees. Bending is shown to be associated with a number of A-tracts in the upstream sequence. However, altering the phasing of the A-tracts has little effect on the affinity for AhrC. Truncation and competition experiments have been used to investigate the possible role of sequences flanking the operator on affinity. Very surprisingly, the affinity of the O(rocA) site appears to increase in the presence of excess, specific competitor fragment, i.e. the system shows anti-competitive effects. Competition is restored at high molar excesses of specific fragment over the protein. We propose a novel model for the assembly of a higher affinity form of AhrC at operator sites that is consistent with both the apparent co-operativity of binding and the anti-competitive effects. These data suggest that the molecular interactions occurring between the prokaryotic arginine-regulatory proteins and their operators may be more complex than is generally appreciated.
Asunto(s)
Bacillus subtilis/metabolismo , Proteínas Bacterianas , Proteínas de Escherichia coli , Regiones Operadoras Genéticas , Proteínas Represoras/metabolismo , Transactivadores/genética , Transactivadores/metabolismo , Arginina/metabolismo , Bacillus subtilis/genética , Secuencia de Bases , Sitios de Unión , Clonación Molecular , Secuencia de Consenso/genética , Huella de ADN , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Oligodesoxirribonucleótidos/genética , Oligodesoxirribonucleótidos/metabolismo , Proteínas Recombinantes/metabolismo , Transcripción Genética/genéticaRESUMEN
Previous studies have suggested that inducible erythromycin (Er) resistance in staphylococci mediated by the plasmid-borne ABC-transporter msrA is dependent on additional unidentified chromosomally encoded transmembrane (TM) domains. The requirement for two S. aureus candidate sequences, stpC and smpC, highly similar to sequences adjacent to msrA on the original S. epidermidis plasmid was investigated. Deletion of the sequences by allelic replacement was accomplished by electroporation of S. aureus RN4220 with a nonreplicating suicide vector. S. aureus strains carrying a delta(stpC-smpC) mutation showed an identical ErR phenotype to those arising from single crossover events and unmutated RN4220 containing msrA. This proves that neither stpC nor smpC is required for ErR. To further define the minimal functional unit required for MSR, the control region within the leader sequence of msrA was deleted. This resulted in constitutive resistance to Er and type B streptogramins (Sg), proving that SgR does not require the presence of Er. Deletion constructs containing the N- or C-terminal ABC regions of MsrA did not confer ErR in RN4220 singly or in combination.
Asunto(s)
Antibacterianos/farmacología , Proteínas Bacterianas/genética , Eritromicina/farmacología , Regulación Bacteriana de la Expresión Génica/genética , Proteínas de Transporte de Membrana , Staphylococcus aureus/efectos de los fármacos , Proteínas Bacterianas/fisiología , Proteínas Portadoras/genética , Proteínas Portadoras/fisiología , Cromosomas Bacterianos/genética , Intercambio Genético , Farmacorresistencia Microbiana/genética , Proteínas de la Membrana/genética , Proteínas de la Membrana/fisiología , Fenotipo , Eliminación de Secuencia , Staphylococcus aureus/genética , Virginiamicina/farmacologíaRESUMEN
Mechanisms of gene regulation have not yet been extensively studied in thermophilic bacteria. In previous studies we showed that the Bacillus stearothermophilus argCJBD gene cluster is subject to specific repression by arginine. Here we report the cloning by colony hybridization, and characterization of the proximal part of the argC gene together with the adjacent control region of the cluster. The promoter was identified by primer extension mapping of the argC transcription startpoint: a sequence overlapping it was found to be similar to the arginine operators of B. subtilis and to a smaller extent of E. coli. Use of an argC-lacZ gene fusion revealed that the argC promoter is strongly repressed by the heterologous B. subtilis arginine repressor/activator AhrC in E. coli cells. Mobility shift and DNase I footprinting experiments revealed tight, specific and arginine-dependent binding of this operator-like sequence to purified AhrC. It is therefore very likely that in B. stearothermophilus the expression of the argCJBD operon is modulated by a repressor that is the thermophilic homologue of AhrC.
Asunto(s)
Arginina/biosíntesis , Bacillus subtilis/genética , Proteínas Bacterianas , Proteínas de Escherichia coli , Geobacillus stearothermophilus/genética , Operón/genética , Proteínas Represoras/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Huella de ADN , ADN Recombinante , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica , Datos de Secuencia Molecular , Regiones Operadoras Genéticas/genética , Regiones Promotoras Genéticas/genética , Proteínas Represoras/genética , Mapeo Restrictivo , Análisis de Secuencia de ADN , Transformación BacterianaRESUMEN
In streptomycetes, the branched-chain amino acids leucine, isoleucine and valine may serve as precursors for commercially important polyketides, and it is of interest to investigate whether the availability of these amino acids affects the production of the secondary metabolites derived from them. This paper reports studies on end-product control in the model organism Streptomyces coelicolor of the enzymes acetohydroxy acid synthase (AHAS) and isopropylmalate synthase (IPMS), mediating steps in the pathways to isoleucine-valine and leucine respectively. Specific activities of both enzymes were similarly affected when minimal medium was supplemented with the amino acids singly or in combination. Isoleucine alone caused a 2- to 3-fold increase, while all three amino acids caused a 5- to 8-fold decrease. Growth of an ilv auxotroph in media with limiting isoleucine gave enzyme specific activities 4- to 6-fold higher than in unsupplemented minimal medium. Spontaneous mutants were obtained by growing S. coelicolor on minimal medium containing 4-azaleucine. At lease four patterns of end-product control were found among the mutants, one of which showed high constitutive levels of both enzymes (7- and 15-fold above unsupplemented minimal medium values for AHAS and IPMS respectively). It is concluded that the variation in specific activities of the two enzymes under different physiological and genetic conditions spans a range of around 50 to 100, and that S. coelicolor has molecular mechanisms capable of producing this response.
Asunto(s)
2-Isopropilmalato Sintasa/metabolismo , Acetolactato Sintasa/metabolismo , Aminoácidos de Cadena Ramificada/biosíntesis , Aminoácidos/farmacología , Streptomyces/enzimología , Aminoácidos/metabolismo , Medios de Cultivo , Retroalimentación , Isoleucina/metabolismo , Cinética , Leucina/análogos & derivados , Leucina/metabolismo , Leucina/farmacología , Modelos Biológicos , Valina/metabolismoRESUMEN
As with most amino acid biosynthetic pathways in streptomycetes, enzymes of arginine biosynthesis in Streptomyces coelicolor show only slight derepression in minimal medium without, as opposed to with, exogenous arginine. However, when an arginine auxotroph was cultured in limiting arginine, ornithine carbamoyltransferase (OCT) activities rose by as much as 100-fold. The response was not due to a general starvation effect. To elucidate the repression-derepression mechanism, a DNA fragment containing the upstream region of the previously isolated S. coelicolor argCJB cluster was cloned into a multicopy vector and transformed into wild-type S. coelicolor; a slight transient derepression of OCT was observed in minimal medium without, though not with, added arginine, consistent with titration by the insert of a negatively acting macromolecule such as a repressor. A subfragment carrying the 5' end of argC and the region immediately upstream showed specific binding, in mobility shift assays, to purified AhrC, the repressor/activator of genes of arginine metabolism in Bacillus subtilis. It is therefore likely that in S. coelicolor, expression of arginine biosynthesis genes is controlled by a protein homologous to the well-characterised B. subtilis and Escherichia coli repressors.
Asunto(s)
Arginina/biosíntesis , Proteínas de Escherichia coli , Regulación Bacteriana de la Expresión Génica , Ornitina Carbamoiltransferasa/metabolismo , Streptomyces/genética , Arginina/genética , Proteínas Bacterianas/fisiología , Medios de Cultivo/metabolismo , Regiones Promotoras Genéticas , Proteínas Represoras/fisiología , Streptomyces/enzimologíaRESUMEN
Streptomyces griseus ATCC 12475 fails to produce streptomycin when grown at 34 degrees C or above, although growth is appreciable up to at least 37 degrees C. This depression of streptomycin production at elevated growth temperature is manifest equally in liquid and on solid, and with complex and minimal, media. We report studies with gene fusions of the reporter genes aph or xyIE to restriction fragments containing the streptomycin biosynthesis promoter PstrB1. aph constructs were in high, and xyIE constructs in low, copy number vectors. Two strB1 promoter fragments were used, one requiring activation by the pathway-specific activator StrR of S. griseus, the other reportedly activator independent. PstrB1 expression in the aph constructs in S. griseus and in S. lividans was significantly reduced at 37 degrees C compared to 30 degrees C. Some of this reduction could be explained by lower plasmid copy number at the higher temperature, but strR-dependent expression was clearly temperature controlled. Using the xyIE reporter system, the temperature dependence of PstrB1 expression was confirmed but, surprisingly, the strR dependence of the two promoter fragments differed from that observed in the multicopy aph constructs. These data identify a temperature-dependent promoter which may contribute to the depressive effect of elevated growth temperature on streptomycin production.
Asunto(s)
Dioxigenasas , Regulación Bacteriana de la Expresión Génica , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Regiones Promotoras Genéticas/genética , Streptomyces griseus/metabolismo , Estreptomicina/biosíntesis , Catecol 2,3-Dioxigenasa , Genes Reporteros , Calor/efectos adversos , Oxigenasas/análisis , Plásmidos/genética , Proteínas Recombinantes de Fusión/biosíntesis , Streptomyces griseus/genéticaRESUMEN
A gene (ertX) encoding a putative ABC transporter was cloned from the erythromycin producer Saccharopolyspora erythraea, using PCR. The primers were based on regions of homology from ABC transporters which confer resistance to macrolide antibiotics. While ertX encodes a protein with a strong degree of similarity to other macrolide ABC transporters from streptomycetes and staphylococci, it did not confer resistance to erythromycin, tylosin, spiramycin, oleandomycin, josamcin, chalcomycin or midecamycin when subcloned into sensitive streptomycete hosts. Southern blot analysis suggested that ertX did not constitute part of the erythromycin gene cluster as identified to date.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Reacción en Cadena de la Polimerasa/métodos , Saccharopolyspora/genética , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , ADN Bacteriano/genética , Farmacorresistencia Microbiana/genética , Eritromicina/farmacología , Genes Bacterianos , Datos de Secuencia Molecular , Filogenia , Saccharopolyspora/efectos de los fármacos , Homología de Secuencia de AminoácidoRESUMEN
In Bacillus subtilis, the AhrC protein represses genes encoding enzymes of arginine biosynthesis and activates those mediating its catabolism. To determine how this repressor also functions as an activator, we attempted to clone catabolic genes by searching for insertions of the Tn917-lacZ transposon that express AhrC-dependent, arginine-inducible beta-galactosidase activity. One such isolate was obtained. The region upstream of lacZ was subcloned in Escherichia coli in such a way that it could be replaced in the B. subtilis chromosome after appropriate manipulation. Analysis of exonuclease III-derived deletions located an AhrC-dependent, arginine-inducible promoter to within a ca. 1.9 kb fragment. The sequence revealed: the 3' end of an ORF homologous to gdh genes encoding glutamate dehydrogenase, with highest homology to the homologue from Clostridium difficile; the 5' end of an ORF homologous to a Saccharomyces cerevisiae gene encoding delta 1-pyrroline 5-carboxylate dehydrogenase (P5CDH), an enzyme of arginine catabolism; and just upstream of the latter, a sequence with homology to known AhrC binding sites in the upstream part of the biosynthetic argCJBD-cpa-F cluster. The same region has also been sequenced by others as part of the B. subtilis genome sequencing project, revealing that the P5CDH gene is the first in a cluster termed rocABC. Restriction fragments containing the putative AhrC-binding sequence, but not those lacking it, showed retarded electrophoretic mobility in the presence of purified AhrC. A 277 bp AhrC-binding fragment also showed anomalous mobility in the absence of AhrC, consistent with its being intrinsically bent. DNAse I footprinting localized AhrC binding to bp -16/-22 to +1 (the transcription startpoint). Such a location for an activator binding site, i.e. overlapping the transcription start, is unusual.
Asunto(s)
Arginina/metabolismo , Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Secuencia de Aminoácidos , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Sitios de Unión , Elementos Transponibles de ADN , ADN Bacteriano/química , ADN Bacteriano/genética , Desoxirribonucleasa I/química , Desoxirribonucleasa I/metabolismo , Regulación Bacteriana de la Expresión Génica , Marcadores Genéticos , Datos de Secuencia Molecular , Operón , Plásmidos/genética , Proteínas Represoras/química , Análisis de Secuencia de ADN , Eliminación de Secuencia , Homología de Secuencia de Ácido Nucleico , Transformación Genética , beta-Galactosidasa/genéticaRESUMEN
The adjacent genes strR-strA-strB1 lie within the large cluster of genes of streptomycin biosynthesis and resistance in Streptomyces griseus. strR encodes a pathway-specific activator StrR, suggested by previous work to be either an antiterminator or a conventional activator, binding to its DNA target via a helix-turn-helix motif. strB1 is transcribed in an StrR-dependent fashion from a promoter (PstrB1) that lies downstream from strA; between PstrB1 and strB1 there is a 300-bp leader region containing numerous inverted repeats that could represent modulatable transcription termination sites. Hybrid plasmids were constructed in vitro with transcriptional fusions in which fragments containing PstrB1 and either the entire leader region ("long" fragments) or a small part of it (the "short" fragment) were cloned upstream of (i) aph as reporter gene, in a high copy number plasmid background, or (ii) xylE as reporter gene, in a low copy number plasmid background. The short fragment directed high levels of APH (aminoglycoside 3'-phosphotransferase) whether StrR was present or not, while the long fragments did not do so in the absence of StrR; one long fragment directed high levels in wild-type S. griseus, in which StrR would be present. Insertion of an extraneous fragment into PstrB1 in the short fragment construct led to loss of APH activity, demonstrating that no adventitious promoter had been formed in the short construct. In vitro deletion of part of the leader region in a long fragment construct led to high APH expression with or without StrR present. Although these results are consistent with the target of StrR being within the leader region, and thus with an antiterminator role, it was found that both long and short fragments in the low copy number background failed to direct high expression of catechol oxygenase (the product of xylE) unless strR was also present on a compatible plasmid. Transfer of PstrB1-xylE fragments to the high copy number vector did not increase catechol oxygenase expression. We interpret these results in terms of an effect, in the hybrid constructs, of one of the reporter genes on promoter function, possibly by affecting local DNA topology.
Asunto(s)
Proteínas Bacterianas , Dioxigenasas , Regulación Fúngica de la Expresión Génica , Genes Reporteros , Regiones Promotoras Genéticas , Streptomyces griseus/genética , Estreptomicina/biosíntesis , Factores de Transcripción , Secuencia de Bases , Catecol 2,3-Dioxigenasa , ADN de Hongos/química , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Vectores Genéticos , Datos de Secuencia Molecular , Oxigenasas/biosíntesis , Oxigenasas/genética , Fosfotransferasas (Aceptor de Grupo Alcohol)/biosíntesis , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Mapeo Restrictivo , Eliminación de Secuencia , Streptomyces/genética , Streptomyces/metabolismo , Streptomyces griseus/metabolismoRESUMEN
Conditions of growth are described for the production of streptomycin by Streptomyces griseus ATCC 12475 using chemically defined minimal medium and complex medium. It was found using batch cultures that early synthesis of the antibiotic occurred during growth in minimal medium but was delayed until the onset of stationary phase in complex medium. This effect was independent of whether spores or vegetative cells were used as inoculum. Stability of streptomycin biosynthesis in continuous culture was dependent on dilution rate and medium employed. Cultures were highly unstable when grown on complex medium but could be maintained in steady states in continuous culture using minimal medium when the dilution rate was increased in a stepwise manner, starting at a dilution rate of 0.02 h-1 (15% of mumax). The effect of changing dilution rate on growth, streptomycin production and the level of streptomycin phosphotransferase was examined using this technique.
Asunto(s)
Streptomyces griseus/fisiología , Estreptomicina/biosíntesis , División Celular , Medios de Cultivo , Fosfotransferasas (Aceptor de Grupo Alcohol)/biosíntesis , Streptomyces griseus/enzimología , Streptomyces griseus/crecimiento & desarrolloRESUMEN
The energy-dependent efflux of erythromycin (Er) in staphylococci is due to the presence of msr A, which encodes an ATP-binding protein. MsrA is related to the multi-component ATP-binding cassette (ABC) transporters which characteristically also contain membrane-spanning domains. Since MsrA functions in a heterologous host in the absence of other plasmid-encoded products, the requirement for a transmembrane (TM) complex might be fulfilled by hijacking a chromosomally encoded protein. Two genes, stpA and smpA, were identified upstream from msrA on the original Staphylococcus epidermidis plasmid, encoding an ATP-binding protein and a hydrophobic TM protein, respectively. Sequences highly similar to stpA and smpA (stpB and smpB) were also found adjacent to a chromosomal copy of msrA in S. hominis. In Southern blots, internal fragments of stpA or smpA hybridized to the chromosome of the Ers S. aureus RN4220. Cloning and sequence analysis of the region identified revealed the presence of two genes, stpC and smpC, related to stpA and smpA. The deduced amino-acid sequences of the gene products showed that StpA and StpC were 85% identical, whereas SmpA and SmpC were 65% identical. A gene similar to msrA was not present in the S. aureus chromosome. There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact.
Asunto(s)
Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/genética , Eritromicina/metabolismo , Genes Bacterianos , Proteínas de la Membrana/genética , Proteínas de Transporte de Membrana , Staphylococcus/genética , Secuencia de Aminoácidos , Secuencia de Bases , Proteínas Portadoras/metabolismo , Cromosomas Bacterianos , Clonación Molecular , Proteínas de la Membrana/metabolismo , Datos de Secuencia Molecular , Alineación de Secuencia , Homología de Secuencia , Staphylococcus/metabolismo , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo , Staphylococcus epidermidis/genética , Staphylococcus epidermidis/metabolismoAsunto(s)
Adenosina Trifosfatasas , Proteínas Bacterianas/metabolismo , Proteínas de Unión al ADN , Proteínas de Escherichia coli , Mutación , Ácidos Nucleicos Heterodúplex/genética , Secuencia de Bases , Regulador de Conductancia de Transmembrana de Fibrosis Quística , ADN , Reparación del ADN , Exonucleasas/metabolismo , Técnicas Genéticas , Humanos , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Proteína MutS de Unión a los Apareamientos Incorrectos del ADN , Ácidos Nucleicos Heterodúplex/metabolismo , Unión ProteicaRESUMEN
From a partial Sau3AI library of Streptomyces coelicolor A3(2) DNA in pIJ916, two hybrid plasmids pGX1 and pGX2 were isolated that complemented S. coelicolor A3(2) or S. lividans arginine auxotrophs. Subcloning DNA from pGX1 in the Escherichia coli expression vector pRK9 containing the Serratia marcescens trp promoter gave rise to one plasmid, pZC2, that complemented E. coli argB, C, E and H auxotrophs, and another, pZC1, that complemented only the first three. The plasmids were markedly unstable in the various complemented hosts, to varying extents; pZC1 was characterized further as providing the stablest host/plasmid combinations. In vitro deletion of part of the vector's trp promoter did not affect complementation of the argB and C auxotrophs, implying that the S. coelicolor A3(2) arg genes may be expressed from their own promoter. The trp promoter-less plasmids included isolates, such as pZC177, that had suffered extensive further deletion without loss of complementing ability. Extracts of an E. coli argE auxotroph carrying pZC177 showed ornithine acetyltransferase activity, indicating that the complementing gene is of the argJ type. The complementation properties of in vitro deletion derivatives of pZC177 indicated the gene order argC-J-B. Part of argC and the upstream region were sequenced; an ORF was identified whose predicted product showed appreciable homology with the E. coli and Bacillus subtilis ArgC polypeptide. Upstream of this ORF a consensus-type promoter and ribosome binding site could be discerned; overlapping its promoter was a sequence with homology to arginine operators in these two other organisms.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Acetiltransferasas/genética , Aldehído Oxidorreductasas/genética , Proteínas Bacterianas/genética , Genes Bacterianos , Proteínas Recombinantes de Fusión/genética , Streptomyces/genética , Acetiltransferasas/biosíntesis , Aldehído Oxidorreductasas/biosíntesis , Secuencia de Aminoácidos , N-Acetiltransferasa de Aminoácidos , Proteínas Bacterianas/biosíntesis , Secuencia de Bases , Clonación Molecular , ADN Bacteriano/genética , Escherichia coli , Regulación Bacteriana de la Expresión Génica , Prueba de Complementación Genética , Vectores Genéticos , Datos de Secuencia Molecular , Familia de Multigenes , Plásmidos , Regiones Promotoras Genéticas , Proteínas Recombinantes de Fusión/biosíntesis , Transcripción GenéticaRESUMEN
We have determined the nucleotide (nt) sequence of nat1, a gene encoding nourseothricin (Nc) acetyltransferase (AT) from Streptomyces noursei, and its transcriptional start point (tsp). The nt sequence upstream from the coding region is completely different from that of the stat gene (encoding streptothricin AT) from Streptomyces lavendulae [S. Horinouchi, K. Furuya, M. Nishiyama, H. Suzuki and T. Beppu, J. Bacteriol. 169 (1987) 1929-1937], even though the nt sequences of the two genes and the deduced amino acid (aa) sequences of the two enzymes show a high degree of similarity. Another stat gene, derived from a Gram-negative plasmid, showed only deduced aa similarity, but not nt sequence similarity, to the above two. A database search for related aa sequences did not reveal any clear-cut homologies to other types of protein. A multiple aa sequence alignment of several ATs is presented.
Asunto(s)
Acetiltransferasas/genética , Genes Bacterianos , Streptomyces/genética , Transcripción Genética , Acetiltransferasas/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , ADN Bacteriano , Datos de Secuencia Molecular , Mapeo Restrictivo , Homología de Secuencia de Aminoácido , Streptomyces/enzimologíaRESUMEN
The arginine-dependent repressor-activator from Bacillus subtilis, AhrC, has been overexpressed in Escherichia coli and purified to homogeneity. AhrC, expressed in E. coli, is able to repress a Bacillus promoter (argCp), which lies upstream of the argC gene. The purified protein is a hexamer with a subunit molecular mass of 16.7 kDa. Its ability to recognize DNA has been examined in vitro using argCp in both DNase I and hydroxyl radical protection assays. AhrC binds at two distinct sites within the argCp fragment. One site, argCo1, with the highest affinity for protein, is located within the 5' promoter sequences, whilst the other, argCo2, is within the coding region of argC. The data are consistent with the binding of a single hexamer of AhrC to argCo1 via four of its subunits, possibly allowing the remaining two subunits to bind at argCo2 in vivo forming a repression loop similar to those observed for the E. coli Lac repressor.