Perfil dos casos suspeitos de intoxicação exógena em cães e gatos atendidos no hvu ufpi de Teresina-Pi no período de 2017 a 2021 / Profile of suspected cases of exogenous intoxication in dogs and cats treated at the hvu ufpi in Teresina-Pi from 2017 to 2021 / Perfil de los casos sospechosos de intoxicación exógena en perros y gatos atendidos en el hvu ufpi de Teresina-Pi de 2017 a 2021

Os casos suspeitos de intoxicações exógenas são frequentes na clínica de cães e gatos. O presente trabalho objetivou realizar um estudo retrospectivo dos casos suspeitos de intoxicação em cães e gatos atendidos no Hospital Veterinário Universitário da UFPI, localizado em Teresina PI, durante o período de 2017 a 2021. Os casos foram classificados por agente tóxico suspeito, espécie, idade e ano de ocorrência. No total foram registrados 324 casos, sendo 58 em 2017, 88 em 2018, 94 em 2019, 50 em 2020 e 34 em 2021. A maioria dos casos ocorreu em 2019 (29,01%) tendo como causa principal a intoxicação medicamentosa (16,04%), seguida da intoxicação por agrotóxicos (10,80%). A maior prevalência foi em animais com idade inferior a cinco anos (87,03%); machos (56,79%); cães (68,20%). Foram observados: elevado número de casos sem agente tóxico identificado (28,08%) e de fichas de atendimento não preenchidas (8,33%) pelos médicos veterinários em atendimento. A prevenção, a educação continuada dos tutores e a capacitação dos médicos veterinários são destacadas como essenciais para a redução dos casos de intoxicações exógenas em cães e gatos.(AU)

Suspected cases of exogenous poisoning are frequent in the clinic of dogs and cats. The present study aimed to perform a retrospective study of suspected cases of intoxication in dogs and cats treated at the University Veterinary Hospital of UFPI, located in Teresina PI, during the period from 2017 to 2021. The cases were classified by suspected toxic agent, species, age and year of occurrence. A total of 324 cases were recorded, being 58 in 2017, 88 in 2018, 94 in 2019, 50 in 2020 and 34 in 2021. Most cases occurred in 2019 (29.01%) with drug intoxication (16.04%) as the main cause, followed by pesticide intoxication (10.80%). The highest prevalence was in animals less than five years old (87.03%); males (56.79%); dogs (68.20%). It was observed: high number of cases without identified toxic agent (28.08%) and unfilled attendance forms (8.33%) by the attending veterinarians. Prevention, continued education of guardians and training of veterinarians are highlighted as essential for the reduction of cases of exogenous poisoning in dogs and cats.(AU)

Los casos sospechosos de intoxicaciones exógenas son frecuentes en la clínica de perros y gatos. El presente estudio tuvo como objetivo realizar un estudio retrospectivo de los casos sospechosos de intoxicación en perros y gatos tratados en el Hospital Veterinario Universitario de la UFPI, ubicado en Teresina PI, durante el período de 2017 a 2021. Los casos se clasificaron por agente tóxico sospechoso, especie, edad y año de ocurrencia. Se registraron un total de 324 casos, 58 en 2017, 88 en 2018, 94 en 2019, 50 en 2020 y 34 en 2021. La mayoría de los casos se produjeron en 2019 (29,01%) siendo la intoxicación por fármacos la principal causa (16,04%), seguida de la intoxicación por plaguicidas (10,80%). La mayor prevalencia se dio en animales menores de cinco años (87,03%); machos (56,79%); perros (68,20%). Se observó un elevado número de casos sin agente tóxico identificado (28,08%) y de formularios asistenciales no cumplimentados (8,33%) por los veterinarios asistentes. Se insiste en que la prevención, la educación continuada de los propietarios de animales de compañía y la formación de los veterinarios son esenciales para reducir los casos de intoxicación exógena en perros y gatos.(AU)

Physical and hygienic-sanitary conditions of poultry slaughterhouses in the municipality of Parnaíba, Piauí, Brazil / Condições físicas e higiênico-sanitárias dos estabelecimentos de abate de frangos do município de Parnaíba, Piauí, Brasil

This study aimed to evaluate the physical and hygienic-sanitary conditions of poultry slaughterhouses in the municipality of Parnaíba, Piauí, Brazil. Ten slaughterhouses were visited, using a checklist as an evaluation criterion, seeking to assess the physical and hygienic-sanitary conditions of facilities, equipment, handlers, environment, production, and food transportation, during the entire slaughtering process, to verify compliance with current legislation. The slaughterhouses visited (10 / 100%) had no Official Inspection Service and did not obey the rules established by legislation, showing precarious physical and hygienic-sanitary conditions of operation. Most of the establishments (9 / 90%) were located in the urban area and one (10%) in the rural area. None of them followed the animal welfare recommendations. Moreover, seven (70%) establishments had no waste treatment and the residues were dumped in an open area. Therefore, it was concluded that the hygienic-sanitary conditions of poultry slaughterhouses in Parnaíba pose risks to meet quality and the health of slaughterhouses employees and people who consume the products from those establishments.(AU)

Objetivou-se avaliar as condições físicas e higiênico-sanitárias dos estabelecimentos de abate de frango, no muni-cípio de Parnaíba, estado do Piauí. Foram visitados 10 estabelecimentos de abate, sendo utilizado como critério avaliativo da pesquisa um checklist, que buscou avaliar as condições físicas e higiênico-sanitárias das instalações, equipamentos, dos mani-puladores, ambiente, como também, da produção, assim como, transporte do alimento durante todo o processo de abate para averiguar conformidade com a legislação vigente. Todos os abatedouros visitados (10) 100% não apresentavam Serviço de Inspeção Oficial e encontravam-se fora das normas estabelecidas pela legislação, com condições físicas e higiênico-sanitárias precárias de funcionamento. A grande maioria dos estabelecimentos, (9) 90% encontravam-se localizados na zona urbana e um (10%) na zona rural. Estes não seguiam as recomendações de bem-estar animal voltado as atividades. Ainda, (7) 70% dos estabelecimentos não realizavam tratamento dos resíduos, sendo despejados a céu aberto. Portanto, concluiu-se que as condi-ções higiênico-sanitárias dos abatedouros da cidade de Parnaíba proporcionam riscos à qualidade da carne, à saúde dos funcio-nários dos abatedouros e da população que consome os produtos provenientes desses estabelecimentos.(AU)

Physical and hygienic-sanitary conditions of poultry slaughterhouses in the municipality of Parnaíba, Piauí, Brazil / Condições físicas e higiênico-sanitárias dos estabelecimentos de abate de frangos do município de Parnaíba, Piauí, Brasil

This study aimed to evaluate the physical and hygienic-sanitary conditions of poultry slaughterhouses in the municipality of Parnaíba, Piauí, Brazil. Ten slaughterhouses were visited, using a checklist as an evaluation criterion, seeking to assess the physical and hygienic-sanitary conditions of facilities, equipment, handlers, environment, production, and food transportation, during the entire slaughtering process, to verify compliance with current legislation. The slaughterhouses visited (10 / 100%) had no Official Inspection Service and did not obey the rules established by legislation, showing precarious physical and hygienic-sanitary conditions of operation. Most of the establishments (9 / 90%) were located in the urban area and one (10%) in the rural area. None of them followed the animal welfare recommendations. Moreover, seven (70%) establishments had no waste treatment and the residues were dumped in an open area. Therefore, it was concluded that the hygienic-sanitary conditions of poultry slaughterhouses in Parnaíba pose risks to meet quality and the health of slaughterhouses employees and people who consume the products from those establishments.

Objetivou-se avaliar as condições físicas e higiênico-sanitárias dos estabelecimentos de abate de frango, no muni-cípio de Parnaíba, estado do Piauí. Foram visitados 10 estabelecimentos de abate, sendo utilizado como critério avaliativo da pesquisa um checklist, que buscou avaliar as condições físicas e higiênico-sanitárias das instalações, equipamentos, dos mani-puladores, ambiente, como também, da produção, assim como, transporte do alimento durante todo o processo de abate para averiguar conformidade com a legislação vigente. Todos os abatedouros visitados (10) 100% não apresentavam Serviço de Inspeção Oficial e encontravam-se fora das normas estabelecidas pela legislação, com condições físicas e higiênico-sanitárias precárias de funcionamento. A grande maioria dos estabelecimentos, (9) 90% encontravam-se localizados na zona urbana e um (10%) na zona rural. Estes não seguiam as recomendações de bem-estar animal voltado as atividades. Ainda, (7) 70% dos estabelecimentos não realizavam tratamento dos resíduos, sendo despejados a céu aberto. Portanto, concluiu-se que as condi-ções higiênico-sanitárias dos abatedouros da cidade de Parnaíba proporcionam riscos à qualidade da carne, à saúde dos funcio-nários dos abatedouros e da população que consome os produtos provenientes desses estabelecimentos.

Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin- converting enzyme 2 in goat ovary / Imunolocalização de angiotensina-(1-7), angiotensina II e enzima-conversora de angiotensina 2 em ovário de cabras

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.(AU)

Há evidências crescentes quanto à participação do sistema renina-angiotensina ovariano em processos reprodutivos importantes. A inibição da enzima conversora de angiotensina (ECA) ocasionou aumento na taxa de ovulação e gravidez no protocolo de inseminação artificial por tempo fixo (TFIA). Este estudo teve como objetivo investigar a presença e localização de Ang II, Ang-(1-7) e ECA2 em ovários de cabras e a possibilidade do envolvimento desses peptídeos em resultados anterio-res. Dez ovários de cabras foram coletados em abatedouro, lavados em PBS tamponado, perfundidos com solução inibidora de protease e processados para protocolo de imunohistoquímica. A busca por peptídeos foi realizada usando o método avidina-bio-tina-peroxidase. Foi observada uma forte imunorreatividade para Ang II em células da teca de folículos antrais e corpo lúteo. Os folículos antrais (células da teca), corpo lúteo e citoplasma do oócito nos folículos antrais iniciais exibiram forte imunor-reatividade para Ang-(1-7). Houve forte imunorreatividade para ECA2 no citoplasma das células luteais e células da teca dos folículos antrais. Neste estudo, pela primeira vez, a presença e localização de Ang II, Ang- (1-7) e ECA2 são relatadas em ovário caprino, sugerindo que há participação no desenvolvimento folicular, maturação oocitária e desenvolvimento do corpo lúteo.(AU)

Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin- converting enzyme 2 in goat ovary / Imunolocalização de angiotensina-(1-7), angiotensina II e enzima-conversora de angiotensina 2 em ovário de cabras

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.

Há evidências crescentes quanto à participação do sistema renina-angiotensina ovariano em processos reprodutivos importantes. A inibição da enzima conversora de angiotensina (ECA) ocasionou aumento na taxa de ovulação e gravidez no protocolo de inseminação artificial por tempo fixo (TFIA). Este estudo teve como objetivo investigar a presença e localização de Ang II, Ang-(1-7) e ECA2 em ovários de cabras e a possibilidade do envolvimento desses peptídeos em resultados anterio-res. Dez ovários de cabras foram coletados em abatedouro, lavados em PBS tamponado, perfundidos com solução inibidora de protease e processados para protocolo de imunohistoquímica. A busca por peptídeos foi realizada usando o método avidina-bio-tina-peroxidase. Foi observada uma forte imunorreatividade para Ang II em células da teca de folículos antrais e corpo lúteo. Os folículos antrais (células da teca), corpo lúteo e citoplasma do oócito nos folículos antrais iniciais exibiram forte imunor-reatividade para Ang-(1-7). Houve forte imunorreatividade para ECA2 no citoplasma das células luteais e células da teca dos folículos antrais. Neste estudo, pela primeira vez, a presença e localização de Ang II, Ang- (1-7) e ECA2 são relatadas em ovário caprino, sugerindo que há participação no desenvolvimento folicular, maturação oocitária e desenvolvimento do corpo lúteo.

Serological evidence of Toxoplasma gondii occurrence in naturally infected sheep in the Teresina microregion, Piauí, Brazil

ABSTRACT Toxoplasmosis is a zoonosis of reproductive nature that is transmitted to humans mainly by ingestion of undercooked meat or drinking oocyte-contaminated water. The slaughter of small ruminants in clandestine slaughterhouses and the presence of companion animals in these locations may be epidemiologically important for the dissemination of the disease. The objective of the present study was to determine toxoplasmosis prevalence by researching anti- Toxoplasma gondii antibodies in meat sheep herds from farms in Teresina microregion, Piauí, Brazil. A total of 450 blood samples were collected from sheep of both sexes, belonging to 28 herds from the 14 municipalities that comprise the microregion. The samples were analyzed by the enzyme-linked immunosorbent assay (ELISA), and the presence of anti-T. gondii antibodies was observed in 62% (279/450) of the animals in all the municipalities and on at least one farm in each municipality. The high occurrence of positive animals is an indication of reproductive problems in these herds, characterizing a problem for both sheep rearing and public health.

Serological evidence of Toxoplasma gondii occurrence in naturally infected sheep in the Teresina microregion, Piauí, Brazil

Toxoplasmosis is a zoonosis of reproductive nature that is transmitted to humans mainly by ingestion of undercooked meat or drinking oocyte-contaminated water. The slaughter of small ruminants in clandestine slaughterhouses and the presence of companion animals in these locations may be epidemiologically important for the dissemination of the disease. The objective of the present study was to determine toxoplasmosis prevalence by researching anti- Toxoplasma gondii antibodies in meat sheep herds from farms in Teresina microregion, Piauí, Brazil. A total of 450 blood samples were collected from sheep of both sexes, belonging to 28 herds from the 14 municipalities that comprise the microregion. The samples were analyzed by the enzyme-linked immunosorbent assay (ELISA), and the presence of anti-T. gondii antibodies was observed in 62% (279/450) of the animals in all the municipalities and on at least one farm in each municipality. The high occurrence of positive animals is an indication of reproductive problems in these herds, characterizing a problem for both sheep rearing and public health.

Frequência de vermifugação em equinos atendidos na clínica de grandes animais do HVU em Teresina Piauí / Frequency of deworming in equines treated at the HVU Large Animals Clinic in Teresina Piauí

The present study aimed to evaluate the frequency of deworming in horses treated at the HVU Large Animals Clinic. It was observed that the administered product, the frequency of administration and the date of the last vermifugation. We can see that many of the owners were not sure which product they used in the deworming of the animal, the frequency of administration was diverse according to the season, the date of the last deworming was very different in the results, factors that can contribute to a resistance to the anthelmintic used.(AU)

Frequência de vermifugação em equinos atendidos na clínica de grandes animais do HVU em Teresina – Piauí / Frequency of deworming in equines treated at the HVU Large Animals Clinic in Teresina – Piauí

The present study aimed to evaluate the frequency of deworming in horses treated at the HVU Large Animals Clinic. It was observed that the administered product, the frequency of administration and the date of the last vermifugation. We can see that many of the owners were not sure which product they used in the deworming of the animal, the frequency of administration was diverse according to the season, the date of the last deworming was very different in the results, factors that can contribute to a resistance to the anthelmintic used.

Análise soroepidemiológica e fatores de risco associados à Leptospira spp. em bovinos no estado do Piauí / Serum epidemiological analysis and risk factors associated with Leptospira spp. in cattle in the state of Piauí

Background: Leptospirosis is a cosmopolitan zoonosis caused by pathogenic spirochetes of the genus Leptospira spp. and it is considered one of the main causes of reproductive problems in cattle. Therefore, the aim of this study was to determine the occurrence of anti-Leptospira antibodies and identify the prevalent serovars and risk factors associated with infection in cattle herds, in the microregion of Floriano, Piaui State, Brazil.Materials, Methods & Results: A total of 414 bovine sera samples were collected (390 females aged over 24 months and 24 bulls) from 22 properties (farms) in the municipalities that compose the study area. The samples were analyzed using the Microscopic Agglutination Test (MAT) to detect anti-Leptospira antibodies from 23 pathogenic serovars. An epidemiological questionnaire was applied in each farm to evaluate the risk factors, using a univariate analysis of the variables of interest, by Pearson’s Chi-square test (χ2 ) or Fisher’s exact test, when it was necessary. Then, each independent variable was crossed with the dependent variable and those that presented statistical significance 1: 100) in the 22 evaluated farms; all of them had at least one positive animal, resulting in a prevalence of 34.54%, with 32,8% females (136) and 1,7% males (07), and 8,93% (37) of co-agglutination. Nineteen of the 23 tested serovars were identified; among them, Icterohaemorrhagiae (42.48%), Hardjo (31.2%), Pomona (4.3%), and Castellonis (4.3%) stood out. Absence of quarantine (OR = 16.172, P = 0.024), vaccination (OR = 0.090, P = 0.037) and isolation of diseased animals (OR = 0.006, P = 0.030) were identified, by the multivariate logistic regression analysis, as risk factors for any serovar of Leptospira spp.[...]

Análise soroepidemiológica e fatores de risco associados à Leptospira spp. em bovinos no estado do Piauí / Serum epidemiological analysis and risk factors associated with Leptospira spp. in cattle in the state of Piauí

Background: Leptospirosis is a cosmopolitan zoonosis caused by pathogenic spirochetes of the genus Leptospira spp. and it is considered one of the main causes of reproductive problems in cattle. Therefore, the aim of this study was to determine the occurrence of anti-Leptospira antibodies and identify the prevalent serovars and risk factors associated with infection in cattle herds, in the microregion of Floriano, Piaui State, Brazil.Materials, Methods & Results: A total of 414 bovine sera samples were collected (390 females aged over 24 months and 24 bulls) from 22 properties (farms) in the municipalities that compose the study area. The samples were analyzed using the Microscopic Agglutination Test (MAT) to detect anti-Leptospira antibodies from 23 pathogenic serovars. An epidemiological questionnaire was applied in each farm to evaluate the risk factors, using a univariate analysis of the variables of interest, by Pearsons Chi-square test (χ2 ) or Fishers exact test, when it was necessary. Then, each independent variable was crossed with the dependent variable and those that presented statistical significance 1: 100) in the 22 evaluated farms; all of them had at least one positive animal, resulting in a prevalence of 34.54%, with 32,8% females (136) and 1,7% males (07), and 8,93% (37) of co-agglutination. Nineteen of the 23 tested serovars were identified; among them, Icterohaemorrhagiae (42.48%), Hardjo (31.2%), Pomona (4.3%), and Castellonis (4.3%) stood out. Absence of quarantine (OR = 16.172, P = 0.024), vaccination (OR = 0.090, P = 0.037) and isolation of diseased animals (OR = 0.006, P = 0.030) were identified, by the multivariate logistic regression analysis, as risk factors for any serovar of Leptospira spp.[...](AU)

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.

Efeito da somatotropina bovina recombinante (rbST) em duas diferentes doses sobre a concentração sérica de progesterona (P4) em cabras Sem Padrão Racial Definido (SPRD) submetidas à inseminação artificial em tempo fixo(IATF) / Effect of recombinant bovine somatotropin (rbST) on two different doses on the serum concentration of progesterone (P4) in undefined breed goats submitted to Fixed Time Artificial Insemination (FTAI)

The objective of this work was to evaluate the influence of the association of rbST with a protocol ofFTAI on the concentration of progesterone (P4) in in undefined breed goats. Goats were inseminated followingthe 11-day protocole. In D6, the animals were divided into three experimental groups: GI (n = 42) 250 mg rbST,SC, G-II (n = 39) 125 mg rbST, SC, and G- III (n = 41) ( control). P4 hormonal dosages were performed byELISA on D0, D09, D12 and D21. The results show that treatment with somatotropin at two different dosesassociated with an IATF protocol did not affect the serum progesterone concentration.

Efeito de um inibidor da Enzima Conversora de Angiotensina sobre a esteroidogênese, e Angiotensina II e Angiotensina-(1-7) em cabras superovuladas / Effect of an angiotensin-converting enzyme inhibitor on steroidogenesis, angiotensin II and angiotensin-(1-7) in superovulated goats

Local renin angiotensin (RAS) system has been described in the ovary, which has been implicated invarious reproductive functions. To evaluate the ovarian RAS in, 13 goats were randomly divided into two groups:Enalapril (n = 7) and control (n = 6). Then, they received superovulation protocol. Enalapril further groupreceived subcutaneously (2mg/kg) enalapril maleate (0.4 mg/kg/day). Blood samples were collected on days 3, 6, 9and 11, and follicular fluid samples from pre-ovulatory ovarian follicles after exposure by celiotomy on D11. AngII in serum and follicular fluid were evaluated on days 9 and 12, Ang-(1-7) on day 12 by HPLC and RIA. E2 andP4 concentrations in serum were determined by ELISA. Ang-(1-7) concentrations in plasma was greater on day9 (P < 0.05), important period for the recruitment and follicular selection. This may indicate that these peptidescan an important function in follicular development and oocyte maturation in superovulated goats.

Laceração perineal de 3° grau em égua Quarto de Milha / 3° grade Perineal laceration in mare Quarter horse

The present report describes the occurrence of a third degree perineal laceration of a Quarter Horsemare and the clinical and surgical measures addressed. During the anamnesis it was reported that the femalepresented a dystocic birth with obstetric maneuvers for the removal of a dead colt from the handler. Areproductive clinical examination revealed third degree perineal laceration compromising mucosa of the vulva,vestibule and vagina with presence of hemorrhage, small amount purulent exudate, pnemovagina and presenceof a rectovaginal communication orifice with fecal contamination. The surgical treatment approached wasvulvoplasty for plastic recovery of the vulva, perineum and rectum allied to the use of antibiotic therapy, antiinflammatory,cleaning of the lesion and adequate diet. The treatment used was successful, associated withveterinary monitoring during the pre, trans and postoperative period, thus minimizing the risks of compromisingthe mare's reproductive efficiency.

Imunolocalização de angiotensina-(1-7), angiotensina II e Enzima Conversora de Angiotensina tipo II em ovário de cabra / Immunolocalization of angiotensin-(1-7), angiotensin II and Angiotensin Converting Enzyme II in goat ovary

There is increasing evidence the participation of ovarian renin-angiotensin system in importantreproductive processes. This study aimed to investigate the presence and location of Ang II, Ang-(1-7) and ACE2in goat ovaries. Ten ovaries from goats killed in slaughterhouse were collected, washed in buffered PBS,perfused with protease inhibitor solution and processed for histology standard protocol. The search of peptideswas performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cellsof antral follicles and corpora lutea was observed. Antral follicles (theca cells), corpora lutea and oocytecytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strongimmunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. For the first time,the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, can regulate folliculardevelopment, oocyte maturation and corpus luteum development.

Redução de gestação gemelar em égua Quarto de Milha / Twin pregnancy reduction in mare Quarter Horse

Twin pregnancies are a serious problem due to the economic losses generated by high abortion rates,embryonic developmental failure with stillbirths and perinatal mortality, as well as a decrease in mare fertility,being considered an unusual event in veterinary medicine and presenting itself as Challenge to the veterinaryprofessional. In view of the above, this work reported the treatment adopted after the diagnosis of twinpregnancy in a mare, through the reduction of one of the embryonic vesicles through the ultrasound guidedmanual crushing technique. It is concluded that the crushing of one of the vesicles is an effective procedure inreducing twin pregnancy, especially when performed until the 15th day of gestation.

Imunolocalização de angiotensina-(1-7), angiotensina II e Enzima Conversora de Angiotensina tipo II em ovário de cabra / Immunolocalization of angiotensin-(1-7), angiotensin II and Angiotensin Converting Enzyme II in goat ovary

There is increasing evidence the participation of ovarian renin-angiotensin system in importantreproductive processes. This study aimed to investigate the presence and location of Ang II, Ang-(1-7) and ACE2in goat ovaries. Ten ovaries from goats killed in slaughterhouse were collected, washed in buffered PBS,perfused with protease inhibitor solution and processed for histology standard protocol. The search of peptideswas performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cellsof antral follicles and corpora lutea was observed. Antral follicles (theca cells), corpora lutea and oocytecytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strongimmunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. For the first time,the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, can regulate folliculardevelopment, oocyte maturation and corpus luteum development.(AU)

Redução de gestação gemelar em égua Quarto de Milha / Twin pregnancy reduction in mare Quarter Horse

Twin pregnancies are a serious problem due to the economic losses generated by high abortion rates,embryonic developmental failure with stillbirths and perinatal mortality, as well as a decrease in mare fertility,being considered an unusual event in veterinary medicine and presenting itself as Challenge to the veterinaryprofessional. In view of the above, this work reported the treatment adopted after the diagnosis of twinpregnancy in a mare, through the reduction of one of the embryonic vesicles through the ultrasound guidedmanual crushing technique. It is concluded that the crushing of one of the vesicles is an effective procedure inreducing twin pregnancy, especially when performed until the 15th day of gestation.(AU)

Laceração perineal de 3° grau em égua Quarto de Milha / 3° grade Perineal laceration in mare Quarter horse

The present report describes the occurrence of a third degree perineal laceration of a Quarter Horsemare and the clinical and surgical measures addressed. During the anamnesis it was reported that the femalepresented a dystocic birth with obstetric maneuvers for the removal of a dead colt from the handler. Areproductive clinical examination revealed third degree perineal laceration compromising mucosa of the vulva,vestibule and vagina with presence of hemorrhage, small amount purulent exudate, pnemovagina and presenceof a rectovaginal communication orifice with fecal contamination. The surgical treatment approached wasvulvoplasty for plastic recovery of the vulva, perineum and rectum allied to the use of antibiotic therapy, antiinflammatory,cleaning of the lesion and adequate diet. The treatment used was successful, associated withveterinary monitoring during the pre, trans and postoperative period, thus minimizing the risks of compromisingthe mare's reproductive efficiency.(AU)