Identification of genomic regions and pathways associated with traits related to rumen acidosis in feedlot Nellore cattle.

There may be an increased risk of metabolic disorders, such as rumen acidosis, in cattle fed high-concentrate diets, particularly those from Bos taurus indicus genotypes, which have shown to be more sensitive to ruminal acidification. Therefore, this study aimed to estimate (co)variance components and identify genomic regions and pathways associated with ruminal acidosis in feedlot Nellore cattle fed high-concentrate diets. It was utilized a dataset containing a total of 642 Nellore bulls that were genotyped from seven feedlot nutrition studies. The GGP Indicus 35k panel was used with the single step genome-wide association study methodology in which the effects of the markers were obtained from the genomic values estimated by the GBLUP model. A bivariate model to estimate genetic correlations between the economically important traits and indicator traits for acidosis was used. The traits evaluated in this study that were nutritionally related to rumen acidosis included average daily gain (ADG), final body weight, time spent eating (TSE), time spent ruminating, rumenitis score (RUM), rumen absorptive surface area (ASA), rumen keratinized layer thickness (KER) and hot carcass weight (HCW). The identified candidate genes were mainly involved in the negative or non-regulation of the apoptotic process, salivary secretion, and transmembrane transport. The genetic correlation between HCW and ASA was low positive (0.27 ± 0.23), and between ADG and ASA was high moderate (0.58 ± 0.59). A positive genetic correlation between RUM and all performance traits was observed, and TSE correlated negatively with HCW (-0.33 ± 0.21), ASA (-0.75 ± 0.48), and KER (-0.40 ± 0.27). The genetic association between economically important traits and indicator traits for acidosis suggested that Nellore cattle may be more sensitive to acidosis in feedlot systems.

Ruminal fermentation pattern of acidosis-induced cows fed either monensin or polyclonal antibodies preparation against several ruminal bacteria.

This study was designed to evaluate a spray-dried multivalent polyclonal antibody preparation (PAP) against lactate-producing bacteria as an alternative to monensin (MON) to control ruminal acidification. Holstein cows (677 ± 98 kg) fitted with ruminal cannulas were allocated in an incomplete Latin square design with two 20 days period. Cows were randomly assigned to control (CTL), PAP, or MON treatments. For each period, cows were fed a forage diet in the first 5 days (d-5 to d-1), composed of sugarcane, urea and a mineral supplement, followed by a 74% concentrate diet for 15 days (d 0 to d 14). There were no treatment main effects (P > 0.05) on dry matter intake (DMI) and microbial protein synthesis. However, there was a large peak (P < 0.01) of intake on d 0 (18.29 kg), followed by a large decline on d 1 (3.67 kg). From d2, DMI showed an increasing pattern (8.34 kg) and stabilized around d 8 (12.96 kg). Higher mean pH was measured (P < 0.01) in cattle-fed MON (6.06 vs. PAP = 5.89 and CTL = 5.91). The ruminal NH3-N concentration of CTL-fed cows was lower (P < 0.01) compared to those fed MON or PAP. The molar concentration of acetate and lactate was not affected (P > 0.23) by treatments, but feeding MON increased (P = 0.01) propionate during the first 4 days after the challenge. Feeding MON and PAP reduced (P = 0.01) the molar proportion of butyrate. MON was effective in controlling pH and improved ruminal fermentation of acidosis-induced cows. However, PAP was not effective in controlling acidosis. The acidosis induced by the challenge was caused by the accumulation of SCFAs. Therefore, the real conditions for evaluation of this feed additive were not reached in this experiment, since this PAP was proposed to work against lactate-producing bacteria.

Feedlot performance, rumen and cecum morphometrics of Nellore cattle fed increasing levels of diet starch containing a blend of essential oils and amylase or monensin.

Feed additives used in finishing diets improve energy efficiency in ruminal fermentation, resulting in increased animal performance. However, there is no report evaluating the effect of BEO associated with exogenous α-amylase in response to increased starch content in feedlot diets. Our objective was to evaluate increasing levels of starch in the diet associated with a blend of essential oils plus amylase or sodium Monensin on performance, carcass characteristics, and ruminal and cecal morphometry of feedlot cattle. 210 Nellore bulls were used (initial body weight of 375 ± 13.25), where they were blocked and randomly allocated in 30 pens. The experiment was designed in completely randomized blocks in a 3 × 2 factorial arrangement: three starch levels (25, 35, and 45%), and two additives: a blend of essential oils plus α-amylase (BEO, 90 and 560 mg/kg of DM, respectively) or sodium Monensin (MON, 26 mg/kg DM). The animals were fed once a day at 08:00 ad libitum and underwent an adaptation period of 14 days. The diets consisted of sugarcane bagasse, ground corn, soybean hulls, cottonseed, soybean meal, mineral-vitamin core, and additives. The animals fed BEO35 had higher dry matter intake (P = 0.02) and daily weight gain (P = 0.02). The MON treatment improved feed efficiency (P = 0.02). The treatments BEO35 and BEO45 increased hot carcass weight (P < 0.01). Animals fed BEO presented greater carcass yield (P = 0.01), carcass gain (P < 0.01), rib eye area gain (P = 0.01), and final rib eye area (P = 0.02) when compared to MON. The MON25 treatment improved carcass gain efficiency (P = 0.01), final marbling (P = 0.04), and final subcutaneous fat thickness (P < 0.01). The use of MON reduced the fecal starch% (P < 0.01). Cattle-fed BEO increased rumen absorptive surface area (P = 0.05) and % ASA papilla area (P < 0.01). The MON treatment reduced the cecum lesions score (P = 0.02). Therefore, the use of BEO with 35 and 45% starch increases carcass production with similar biological efficiency as MON; and animals consuming MON25 improve feed efficiency and reduce lesions in the rumen and cecum.

Assessment of corn wet distillers grains fed to crossbred bulls on feeding behavior, rumen morphology, liver abscesses and blood parameters.

Corn ethanol production has been growing in Brazil in the last ten years, generating by-products to feedlot diets. This study evaluates the effects of the inclusion of low-fat corn wet distillers grains (LF-WDG) on feeding behavior, ruminal health, liver abscesses and blood parameters of F1 Angus-Nellore bulls feedlot finished. Our hypothesis is that evaluation of data from feeding behavior, rumen and liver health would help to explain animal performance. In this trail, one-hundred animals were fed for 129 days with diets containing amounts of 0 (control), 15, 30 and 45% of LF-WDG replacing corn grain and soybean meal. Evaluations of fluctuation of dry matter intake (DMI) were carried out. Additionally, feeding behavior data were assessed by monitoring (24-h period) the feeding, rumination, time spent eating (TSE), and time expended on other activities (resting and number of meals per day). Blood variables such as pH, bicarbonate, total CO2 content, and base excess in extracellular fluid (Beecf) were determined. After slaughter, rumen epithelium was classified according to the incidence of lesions (rumenitis) and abnormalities (papillae clumped), and samples were collected for morphology and histology evaluations. Moreover, livers were scored for severity of abscesses as follow: as unabscessed (0), one or two small abscesses (A-), two to four small active abscesses (A) or one or more large, active abscesses (A+). The DMI (kg/day) differed (P = 0.03) among treatments and there is a tendency of 15 and 30 LF-WDG (% DM) had lower %DMI fluctuation compared to 0 or 45%. The TSE increased linearly (P < 0.01) as the amounts of inclusion of LF-WDG increased. Moreover, neutral detergent fiber (NDF) intake, NDF consumption rate and NDF rumination efficiency increased linearly (P < 0.01) in response to LF-WDG feeding. The incidence of rumenitis tended (P = 0.08) to be greater at 45% LF-WDG, while keratin thickness decreased linearly in bulls fed LF-WDG (P < 0.01). The severity of liver abscesses (score A+) increased linearly (P = 0.02). Regarding blood parameters, only Beecf decreased linearly (P < 0.01) in response to LF-WDG feeding. Therefore, the hypothesis of the current study was confirmed. We previous reported that F1 Angus-Nellore bulls fed LF-WDG show greater weight gain (1.94 ± 0.09 kg/day) and final body weight (620 ± 18.8 kg) when compare to control (1.8 ± 0.09 kg/day and 602 ± 18.8 kg, respectively). Here, we conclude that inclusion of 15 to 30% LF-WDG in feedlot diets improved feeding behavior without impairing ruminal health and blood parameters, driving performance and weigh gain of crossbred bulls. However, bulls fed 45% LF-WDG had greater severity of liver abscesses.

The Potential of Shortening the Adaptation of Nellore Cattle to High-Concentrate Diets Using Only Virginiamycin as Sole Feed Additive.

Feedlot cattle are usually adapted to high-concentrate diets containing sodium monensin (MON) in more than 14 days. However, for finishing diets with lower energy content, the use of MON during adaptation may hold dry matter intake (DMI), and virginiamycin (VM) may be an alternative. This study was designed to determine the potential of shortening the adaptation of Nellore cattle to high-concentrate diets using only VM as a sole feed additive relative to feedlot performance, feeding behavior, and ruminal and cecum morphometrics. The experiment was designed as a completely randomized block replicated six times (four animals/pen) in which 120 Nellore bulls (390.4 ± 19.0 kg) were fed in 30 pens for 111 days according to the following treatments: (1) MON and adaptation for 14 days (MON14), (2) MON + VM and adaptation for 14 days (MONVM14), (3) VM and adaptation for 14 days (VM14), (4) VM and adaptation for 9 days (VM9), and (5) VM and adaptation for 6 days (VM6). At the end of the adaptation, 30 animals (n = 1 per pen) were randomly slaughtered for rumen and cecum evaluations. The remaining 90 bulls were harvested at the end of the study. No effects of treatments were observed (P < 0.10) for final body weight, average daily gain (ADG), and hot carcass weight (HCW). Cattle fed VM14 presented a greater (P ≤ 0.03) DMI, expressed as percent of body weight (BW), than animals fed either MON14 or MONVM14; however, cattle fed either MON14 or MONVM14 improved (P ≤ 0.02) the gain-to-feed ratio (G/F) by 10.4 or 8.1%, respectively, when compared to bulls fed VM14. Bulls fed VM14 had smaller (P < 0.05) papillae area (0.34 vs. 0.42 cm2) and rumen absorptive surface area (28.9 vs. 33.8 cm2) than those fed MON14. The shortening of the adaptation period linearly decreased the 12th rib fat (P = 0.02) and biceps femoris fat daily gain (P = 0.02) of Nellore bulls fed only VM, which linearly decreased the final biceps femoris fat thickness (P < 0.01). Feedlot cattle fed VM as a sole feed additive should not be adapted to high-concentrate diets in less than 14 days. Regardless of either adaptation length or feed additive, feedlot cattle need at least 14 days to adapt to finishing diets.

Effects of the association between whole cottonseed and calcium salts of fatty acids on nutrient intake, feedlot performance, and carcass characteristics of Bos indicus animals offered a high-concentrate diet.

This experiment evaluated the effects of feeding whole cottonseed (WC) and/or calcium salts of fatty acids (CSFA) on dry matter intake (DMI), performance, and carcass characteristics of Bos indicus animals receiving a high-concentrate diet during the finishing phase. On day 0, 96 Nellore bulls were blocked according to initial shrunk body weight (BW; 302 ± 26.7 kg) into group pens (four animals/pen) and, within blocks, pens were randomly assigned to receive: 1) 15% of WC and 2% of CSFA (dry matter [DM] basis) of palm, cottonseed, and soybean oil (15WC; n = 6); 2) 10% of WC and 3% of CSFA (DM basis) of palm, cottonseed, and soybean oil (10WC; n = 6); 3) 5% of WC and 4% of CSFA (DM basis) of palm, cottonseed, and soybean oil (5WC; n = 6); and 4) 0% of WC and 5% of CSFA (DM basis) of palm, cottonseed, and soybean oil (0WC; n = 6). Diets were formulated to be isocaloric, isonitrogenous, and isolipidic. Experimental period lasted 108 d; DMI was evaluated daily, whereas blood samples and carcass measurements were obtained on days 0, 55, and 108 of the study. Upon slaughter on day 109, steaks were collected for determination of the chemical and fatty acid (FA) profile of the meat. No treatment effects (P ≥ 0.35) were observed on DMI, performance, average daily gain (ADG), carcass ultrasound measurements, and chemical variables of the steak. Nonetheless, including WC into the diets decreased C12:0, C16:0, C16:1 trans-9, C17:0, C18:0, C18:1 cis-9, C18:2 cis-9, cis-12, C18:3 cis-9, cis-12, cis-15, saturated, and unsaturated FA intake (P < 0.01). Moreover, adding WC increased DMI fluctuation and feed efficiency (FE; P = 0.03) but decreased marbling (P ≤ 0.03). A treatment × day interaction was observed (P < 0.01) for serum leptin concentration, as 10WC animals had greater leptin concentration on day 103 vs. other treatments (P < 0.01). Regarding steak FA profile, WC addition into the diet increased C18:2 cis-7, trans-9 and C18:3 cis-9, cis-12, cis-15 (P < 0.001), whereas saturated FA was quadratically affected (P = 0.02) and unsaturated FA was reduced for 15WC (P < 0.04). In summary, increasing levels of CSFA into isolipidic finishing diets containing WC did not negatively impact feedlot performance but reduced FE and increased marbling scores of B. indicus bulls, demonstrating its feasibility as a technology to improve carcass traits of low-marbling animals.

Evaluation of liquid and powdered forms of polyclonal antibody preparation against Streptococcus bovis and Fusobacterium necrophorum in cattle adapted or not adapted to highly fermentable carbohydrate diets.

OBJECTIVE: Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet. METHODS: A double 3×3 Latin square design was used with three PAP treatments (control, powdered, and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly. RESULTS: Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of Streptococcus bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals. CONCLUSION: Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.

Effects of fatty acid profile of supplements on intake, performance, carcass traits, meat characteristics, and meat sensorial analysis of feedlot Bos indicus bulls offered a high-concentrate diet.

This experiment was designed to evaluate the effects of lipid source and fatty acid (FA) profile on intake, performance, carcass characteristics, expression of enzymes, and sensorial analysis of Bos indicus animals offered a high-concentrate diet. On day 0, 96 noncastrated animals were blocked by initial body weight (400 ± 19.3 kg), randomly allocated to 1 of 24 pens (4 animals/pen), and pens were randomly assigned to receive: 1) control: basal diet composed of whole cottonseed and corn germ as lipid substrates (CONT; n = 6), 2) calcium salts of fatty acids (CSFA) of soybean: CSFA of soybean oil as replacement for whole cottonseed and corn germ (calcium salts of soybean oil [CSSO]; n = 6), 3) CSFA-Blend: CSFA of palm, cottonseed, and soybean oil as replacement for whole cottonseed and corn germ (calcium salts of vegetable oils [CSVO]; n = 6), and 4) Mix: basal diet containing whole cottonseed, corn germ, and CSVO (MIXT; n = 6). Experiment lasted 108 d and performance, ultrasound measurements, as well as carcass characteristics were evaluated. Additionally, meat FA profile, expression of enzymes involved in lipid metabolism, and sensorial analysis were evaluated. No treatment effects were observed on performance variables, ultrasound, and carcass traits (P ≥ 0.22), whereas animals receiving CONT had a greater intake of C10:0, C16:0, C16:1 trans-9, C18:1 cis-9, C18:2, C18:3, total FA, monounsatured FA (MUFA), and polyunsaturated FA (PUFA) vs. CSSO and MIXT (P < 0.05). Conversely, intake ratios of saturated FA (SFA):MUFA and SFA:PUFA were all reduced for CONT vs. other treatments. Meat obtained from CONT animals had greater colorimetric (L*, a*, and b*) values vs. MIXT (P < 0.01). On meat FA profile, CONT increased C18:0 vs. supplementation with calcium salts (P < 0.02) and supplementation with CSSO yielded greater meat concentrations of C18:1 trans-10 and C18:2 CLA intermediates (P < 0.01). Expression of SREBP-1, SCD, and LPL was downregulated for CSSO (P < 0.05). For sensorial analysis, regular flavor was greater (P = 0.01) for CSSO vs. other treatments, but also greater aroma (P = 0.05) vs. CONT and CSVO. In summary, addition of different lipid sources with varying FA profiles into high-concentrate diets did not affect performance and carcass characteristics of B. indicus animals, but supplementation with calcium salts of soybean oil inhibited the mRNA expression of enzymes involved in lipid metabolism, whereas flavor and aroma were positively affected by this lipid source.

Castração de bovinos Nelore na terminação em confinamento / Castration of Nellore cattle in feedlot finishing diets

O objetivo do presente estudo foi avaliar o desempenho e características de carcaça de bovinos Nelore castrados e não castrados terminados em confinamento com dieta de alto concentrado. Trinta e seis bovinos machos da raça Nelore com idade de 24 meses, provenientes de recria em sistema de pastejo contínuo, foram divididos em dois grupos de 18 animais (castrados e não castrados) com peso vivo inicial (PVi) médio de 347,5 kg ± 21,61 kg. O delineamento experimental foi em blocos casualizados, divididos de acordo com o PVi para cada condição sexual avaliada, constituindo desta forma dois tratamentos experimentais: animais castrados e animais não castrados. Cada tratamento foi composto por seis baias (3 animais/baia), sendo estas consideradas as unidades experimentais do estudo. O período de confinamento foi de 112 dias, sendo o abate realizado no 113º dia. Foram avaliados a ingestão de matéria seca (IMS), o ganho de peso diário (GPD), conversão alimentar (CA) e eficiência alimentar (EA). Além disso, foram avaliados a deposição de tecido muscular e adiposo por meio de ultrassonografia. Após o abate dos animais foram determinados o peso de carcaça quente (PCQ), o peso de carcaça fria (PCF) e rendimento de carcaça (RC). Os dados foram analisados utilizando-se o programa PROC MIXED do SAS e teste de Tukey (P ≤ 5%). Não houveram diferenças significativas para as variáveis PVi e IMS em porcentagem de PV. Já as variáveis PVf, GPD, CA e EA apresentaram efeitos significativos, tendo o grupo de animais não castrados melhor desempenho produtivo. Para as avaliações de características de carcaça foram observadas diferenças significativas para PCQ e PCF onde os animais do grupo não castrado também apresentaram melhores pesos de carcaça. Portanto, conclui-se que para sistemas de confinamento com dietas de alto teor de concentrado, a castração de bovinos Nelore não é recomendada, pois interferiu negativamente no desempenho produtivo das carcaças dos animais castrados.(AU)

The objective of the present study was to evaluate the performance and carcass characteristics of Nelore bulls and steers, finished in feedlot with high concentrate diet. Thirty-six male Nelore cattle with 24 months old, from rearing in a continuous grazing system were divided into two groups of 18 animals (castrated and non-castrated) with initial mean body weight (iBW) of 347.5 kg ± 21.61 kg. The experimental design was a randomized complete block according to iBW for each sexual condition evaluated, constituting two experimental treatments: castrated animals and non-castrated animals. Each treatment consisted of six pens (3 animals/pen), these being considered the experimental units. The feedlot period was 112-days and the slaughter was carried out on the 113th day. The dry matter intake (DMI), average daily gain (ADG), feed conversion (F:G) and feed efficiency (G:F) were evaluated. In addition, the deposition of muscular and adipose tissue was evaluated by means of ultrasonography. After slaughtering of the animals the hot carcass weight (HCW), cold carcass weight (CCW) and carcass yield (YG) were determined. The data were analyzed using thePROC MIXED program from SAS and Tukey test (P ≤ 5%). There was no significant difference for the variables iBW and DMI in percentage of BW. The fBW, ADG, F:G and G:F variables had significant effects, and the group of non-castrated animals obtained abetter productive performance. For the carcass traits evaluations, a significant difference was observed for HCW and CCW, where animals from the non-castrated group also had better carcass data. Therefore, it is concluded that for feedlot systems with highconcentrate diets, castration of Nellore cattle is not recommended because it interfered negatively in the productive performance of the castrated animals.(AU)

Castração de bovinos Nelore na terminação em confinamento / Castration of Nellore cattle in feedlot finishing diets

O objetivo do presente estudo foi avaliar o desempenho e características de carcaça de bovinos Nelore castrados e não castrados terminados em confinamento com dieta de alto concentrado. Trinta e seis bovinos machos da raça Nelore com idade de 24 meses, provenientes de recria em sistema de pastejo contínuo, foram divididos em dois grupos de 18 animais (castrados e não castrados) com peso vivo inicial (PVi) médio de 347,5 kg ± 21,61 kg. O delineamento experimental foi em blocos casualizados, divididos de acordo com o PVi para cada condição sexual avaliada, constituindo desta forma dois tratamentos experimentais: animais castrados e animais não castrados. Cada tratamento foi composto por seis baias (3 animais/baia), sendo estas consideradas as unidades experimentais do estudo. O período de confinamento foi de 112 dias, sendo o abate realizado no 113º dia. Foram avaliados a ingestão de matéria seca (IMS), o ganho de peso diário (GPD), conversão alimentar (CA) e eficiência alimentar (EA). Além disso, foram avaliados a deposição de tecido muscular e adiposo por meio de ultrassonografia. Após o abate dos animais foram determinados o peso de carcaça quente (PCQ), o peso de carcaça fria (PCF) e rendimento de carcaça (RC). Os dados foram analisados utilizando-se o programa PROC MIXED do SAS e teste de Tukey (P ≤ 5%). Não houveram diferenças significativas para as variáveis PVi e IMS em porcentagem de PV. Já as variáveis PVf, GPD, CA e EA apresentaram efeitos significativos, tendo o grupo de animais não castrados melhor desempenho produtivo. Para as avaliações de características de carcaça foram observadas diferenças significativas para PCQ e PCF onde os animais do grupo não castrado também apresentaram melhores pesos de carcaça. Portanto, conclui-se que para sistemas de confinamento com dietas de alto teor de concentrado, a castração de bovinos Nelore não é recomendada, pois interferiu negativamente no desempenho produtivo das carcaças dos animais castrados.

The objective of the present study was to evaluate the performance and carcass characteristics of Nelore bulls and steers, finished in feedlot with high concentrate diet. Thirty-six male Nelore cattle with 24 months old, from rearing in a continuous grazing system were divided into two groups of 18 animals (castrated and non-castrated) with initial mean body weight (iBW) of 347.5 kg ± 21.61 kg. The experimental design was a randomized complete block according to iBW for each sexual condition evaluated, constituting two experimental treatments: castrated animals and non-castrated animals. Each treatment consisted of six pens (3 animals/pen), these being considered the experimental units. The feedlot period was 112-days and the slaughter was carried out on the 113th day. The dry matter intake (DMI), average daily gain (ADG), feed conversion (F:G) and feed efficiency (G:F) were evaluated. In addition, the deposition of muscular and adipose tissue was evaluated by means of ultrasonography. After slaughtering of the animals the hot carcass weight (HCW), cold carcass weight (CCW) and carcass yield (YG) were determined. The data were analyzed using thePROC MIXED program from SAS and Tukey test (P ≤ 5%). There was no significant difference for the variables iBW and DMI in percentage of BW. The fBW, ADG, F:G and G:F variables had significant effects, and the group of non-castrated animals obtained abetter productive performance. For the carcass traits evaluations, a significant difference was observed for HCW and CCW, where animals from the non-castrated group also had better carcass data. Therefore, it is concluded that for feedlot systems with highconcentrate diets, castration of Nellore cattle is not recommended because it interfered negatively in the productive performance of the castrated animals.

The inclusion of de-oiled wet distillers grains in feedlot diets reduces the expression of lipogenic genes and fat content in Longissimus muscle from F1 Angus-Nellore cattle.

The inclusion of agro-industry by-products originated from corn ethanol production has increased in animal nutrition in Brazil, reducing formulation costs. In the literature, there is no consensus on how the high inclusion of de-oiled wet distillers grains can affect beef quality and the expression of lipogenic genes in Longissimus muscle. The aim of this study was to evaluate the effects of WDG in the diet of F1 Angus-Nellore cattle on meat quality characteristics, chemical composition and expression of genes involved in lipid metabolism. A hundred F1 Angus-Nellore bulls, with average initial body weight (BW) of 369.5 ± 49 kg were used. The experiment was carried out in a randomized block design, and the animals were divided into two blocks (light and heavy) according to the initial body weight. The animals were fed diets containing levels of 0 (control), 15, 30 and 45% of WDG replacing dry corn and soybean meal. After 129 days of feedlot, the animals were slaughtered and samples of the longissimus thoracis (LT) muscle were collected for quality analyzes such as shear force (3, 10 and 17 aging days), color (luminosity, red, Chroma and Hue), cooking losses, pH and chemical composition (moisture, protein, lipids and ash contents). In addition, the expression of the PPARα, PPARγ, SREBP-1c, SCD1, LPL, FABP4, FASN, ACOX, CPT2, GPX1 and ACACA genes was investigated in the LT muscle by real-time reverse transcription polymerase chain reaction (RT-PCR). Data were analyzed using polynomial contrasts (linear, quadratic and control vs. WDG). There was no interaction (P > 0.05) between aging times and the inclusion of WDG in the diets on the meat quality (pH, cooking losses, coloration and tenderness). However, diets with increasing levels of WDG caused a linear reduction (P = 0.01) in the intramuscular fat of LT. The lipogenic genes SCD1, PPARγ, FASN and CPT2 were less expressed (P < 0.05) in response to the inclusion of WDG. These results suggest that the inclusion of WDG reduced the expression of lipogenic genes and consequently the marbling of LT muscle without affecting tenderness (shear force) and meat color traits.

Influence of lamb finishing system on animal performance and meat quality

This study aimed to assess the influence of lamb finishing systems on zootechnical performance, as well as on carcass and meat quality. The experiment was conducted at the APTAs experimental farm. Thirty-three lambs were used - both sexes, initial age of 90 ± 3 days, Texel with Santa Inês, each animal being one experimental unit, with 6 males and 5 females per treatment. Treatments consisted of: lambs finished on pasture, in semi-feedlot or in feedlot. The lambs were slaughtered with average live weight of 35 kg. Weight gain and carcass measures were taken by ultrasound. After slaughter, carcass conformation and yield, pH, temperature, color, water retention capacity and tenderness were measured. Animals finished on pasture had lower weight gain, were slaughtered at an older age, with lighter carcass weight, smaller loin area, lower shank compactness index, besides lighter shoulder and shank weights, compared to the other production systems (p < 0.05). There was no difference between the semi-feedlot and the feedlot systems for the assessed characteristics. In conclusion, production systems affect animal performance, as well as carcass and meat quality, especially when it comes to important production aspects, such as slaughter age and yield of premium cuts.(AU)

Influence of lamb finishing system on animal performance and meat quality

This study aimed to assess the influence of lamb finishing systems on zootechnical performance, as well as on carcass and meat quality. The experiment was conducted at the APTA’s experimental farm. Thirty-three lambs were used - both sexes, initial age of 90 ± 3 days, Texel with Santa Inês, each animal being one experimental unit, with 6 males and 5 females per treatment. Treatments consisted of: lambs finished on pasture, in semi-feedlot or in feedlot. The lambs were slaughtered with average live weight of 35 kg. Weight gain and carcass measures were taken by ultrasound. After slaughter, carcass conformation and yield, pH, temperature, color, water retention capacity and tenderness were measured. Animals finished on pasture had lower weight gain, were slaughtered at an older age, with lighter carcass weight, smaller loin area, lower shank compactness index, besides lighter shoulder and shank weights, compared to the other production systems (p < 0.05). There was no difference between the semi-feedlot and the feedlot systems for the assessed characteristics. In conclusion, production systems affect animal performance, as well as carcass and meat quality, especially when it comes to important production aspects, such as slaughter age and yield of premium cuts.

Feedlot performance, feeding behavior and rumen morphometrics of Nellore cattle submitted to different feeding frequencies

Owing to the lack of information in the literature about the ideal feeding frequency adopted by feedlot systems, this study was designed to evaluate the effect of different feeding frequencies on performance, carcass traits, feeding behavior and rumen morphometrics of Nellore cattle. Forty-eight yearling bulls were housed in individual pens with an average initial body weight of 358.2 kg. The experiment was designed as a completely randomized block and the treatments were the feeding of cattle one, two, three, or four times daily (n = 12 per treatment). Orthogonal contrasts were used to assess linear, quadratic and cubic relationship between feeding frequency and the dependent variable. As feeding frequency increased, final body weight (p = 0.03) and average daily gain (p = 0.01; one: 0.98 kg d1; two: 0.96 kg d1; three: 1.18 kg d1; four: 1.14 kg d1) increased linearly and the feed:gain ratio decreased (p = 0.02). Also, as feeding frequency increased, hot carcass weight was affected cubically (p = 0.01; one: 246.9 kg; two: 243.0 kg; three: 263.1 kg; four: 258.1 kg). During the adaptation period dry matter intake variation was affected cubically (p 0.05; one: 16 %; two: 23 %; three: 14 %; four: 16 %). Increases in feeding frequency affected papillae height (p 0.01) and papillae surface area (p 0.01) cubically, as well as producing increases in the mitotic index (p = 0.05; one: 9 %; two: 11 %; three: 13 %; four: 13 %) and cells in proliferation (p = 0.05) linearly. Thus, Nellore yearling bulls should be fed three times daily in feedlot operations.

Feedlot performance, feeding behavior and rumen morphometrics of Nellore cattle submitted to different feeding frequencies

Owing to the lack of information in the literature about the ideal feeding frequency adopted by feedlot systems, this study was designed to evaluate the effect of different feeding frequencies on performance, carcass traits, feeding behavior and rumen morphometrics of Nellore cattle. Forty-eight yearling bulls were housed in individual pens with an average initial body weight of 358.2 kg. The experiment was designed as a completely randomized block and the treatments were the feeding of cattle one, two, three, or four times daily (n = 12 per treatment). Orthogonal contrasts were used to assess linear, quadratic and cubic relationship between feeding frequency and the dependent variable. As feeding frequency increased, final body weight (p = 0.03) and average daily gain (p = 0.01; one: 0.98 kg d1; two: 0.96 kg d1; three: 1.18 kg d1; four: 1.14 kg d1) increased linearly and the feed:gain ratio decreased (p = 0.02). Also, as feeding frequency increased, hot carcass weight was affected cubically (p = 0.01; one: 246.9 kg; two: 243.0 kg; three: 263.1 kg; four: 258.1 kg). During the adaptation period dry matter intake variation was affected cubically (p 0.05; one: 16 %; two: 23 %; three: 14 %; four: 16 %). Increases in feeding frequency affected papillae height (p 0.01) and papillae surface area (p 0.01) cubically, as well as producing increases in the mitotic index (p = 0.05; one: 9 %; two: 11 %; three: 13 %; four: 13 %) and cells in proliferation (p = 0.05) linearly. Thus, Nellore yearling bulls should be fed three times daily in feedlot operations.(AU)

Incidência de ruminite e abscesso hepático em bovinos jovens confinados alimentados com dietas contendo aditivos alimentares / Incidence of ruminite and liver abscess of feedlot bullocks fed high concentrate diets containing feed additives

O objetivo deste estudo foi avaliar o efeito da inclusão do preparado de anticorpos policlonais (PAP) e/ou de monensina sódica (MON) na morfologia das papilas ruminais, incidência de ruminites (IR) e abscessos hepáticos (AH) em bovinos jovens confinados. Foram utilizados 72 novilhos da raça Brangus, não castrados, com peso vivo inicial de 261,04 ± 34,73 kg, em 24 baias (3 animais/baia) com 6 repetições por tratamento. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 2 x 2, sendo os fatores a adição ou não de MON e PAP. Após o abate foram realizadas as avaliações. No fígado tanto o percentual de AH quanto a severidade foram menores (P < 0,01) nos animais que receberam PAP em relação aos que não receberam. A inclusão de MON foi apenas eficiente em reduzir (P < 0,01) a severidade dos AH em relação ao tratamento sem o aditivo. Não houve efeito dos aditivos sobre o IR. O tratamento com MON resultou em maior (P=0,07) superfície de absorção/cm2 de parede de rúmen e maior ( P=0,10) área papilar em relação ao tratamento sem este aditivo. Número de papilas/cm2 de parede e área média das papilas não apresentaram diferenças entre tratamentos. O uso de PAP foi eficiente na redução de AH bem como a severidade, porém não foi capaz de melhorar a forma e a estrutura das papilas ruminais, como o observado com o uso de MON.

The aim of this study was to evaluate the effects of polyclonal antibodies preparation (PAP) and/or monensin (MON) on the morphology of the rumen papillae, incidence of ruminate (IR) and liver abscess in feedlot bullocks. Seventy-two bullocks Brangus (261.04 ± 34.73kg) were housed in 24 pens (3 bullocks/pen) with6 replications per treatment. The experimental design was completely randomized 2 x 2 factorial, the factors werethe inclusion or not of MON and PAP.After slaughter, the evaluations were performed. For the livers, both the percentage of abscesses and the severity of them were lower (P 0.05) of additives on the IR. The treatment with MON had higher (P = 0.07) surface absorption/cm2 wall of the rumen and greater (P = 0.10) papillary area compared to treatment without this additive. Number of papillae/cm2 wall and average area of the papillae showed no difference among treatments. The use of PAP was effective in reducing HA as well as the severity the same, but was not able to improve the shape and structure of the ruminal papillae, as observed with the use of MON.

Incidência de ruminite e abscesso hepático em bovinos jovens confinados alimentados com dietas contendo aditivos alimentares

p>The aim of this study was to evaluate the effects of polyclonal antibodies preparation (PAP) and/or monensin (MON) on the morphology of the rumen papillae, incidence of ruminate (IR) and liver abscess in feedlot bullocks. Seventy-two bullocks Brangus (261.04 ± 34.73kg) were housed in 24 pens (3 bullocks/pen) with6 replications per treatment. The experimental design was completely randomized 2 x 2 factorial, the factors werethe inclusion or not of MON and PAP.After slaughter, the evaluations were performed. For the livers, both the percentage of abscesses and the severity of them were lower ( italic>P /italic> 0.01) in animals fed diets with PAP than those fed MON. The inclusion of MON in the diet was only effective in reducing ( italic>P /italic> 0.01) the severity of the abscesses in relation to treatment without this additive. There was no effect ( italic>P /italic>> 0.05) of additives on the IR. The treatment with MON had higher ( italic>P /italic> = 0.07) surface absorption/cm sup>2 /sup> wall of the rumen and greater ( italic>P /italic> = 0.10) papillary area compared to treatment without this additive. Number of papillae/cm sup>2 /sup> wall and average area of the papillae showed no difference among treatments. The use of PAP was effective in reducing HA as well as the severity the same, but was not able to improve the shape and structure of the ruminal papillae, as observed with the use of MON. /p>

p>O objetivo deste estudo foi avaliar o efeito da inclusão do preparado de anticorpos policlonais (PAP) e/ou de monensina sódica (MON) na morfologia das papilas ruminais, incidência de ruminites (IR) e abscessos hepáticos (AH) em bovinos jovens confinados. Foram utilizados 72 novilhos da raça Brangus, não castrados, com peso vivo inicial de 261,04 ± 34,73 kg, em 24 baias (3 animais/baia) com 6 repetições por tratamento. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 2 x 2, sendo os fatores a adição ou não de MON e PAP. Após o abate foram realizadas as avaliações. No fígado tanto o percentual de AH quanto a severidade foram menores (P 0,01) nos animais que receberam PAP em relação aos que não receberam. A inclusão de MON foi apenas eficiente em reduzir (P 0,01) a severidade dos AH em relação ao tratamento sem o aditivo. Não houve efeito dos aditivos sobre o IR. O tratamento com MON resultou em maior (P=0,07) superfície de absorção/cm sup>2 /sup> de parede de rúmen e maior (P=0,10) área papilar em relação ao tratamento sem este aditivo. Número de papilas/cm sup>2 /sup> de parede e área média das papilas não apresentaram diferenças entre tratamentos. O uso de PAP foi eficiente na redução de AH bem como a severidade, porém não foi capaz de melhorar a forma e a estrutura das papilas ruminais, como o observado com o uso de MON. /p>

Incidência de ruminite e abscesso hepático em bovinos jovens confinados alimentados com dietas contendo aditivos alimentares / Incidence of ruminite and liver abscess of feedlot bullocks fed high concentrate diets containing feed additives

O objetivo deste estudo foi avaliar o efeito da inclusão do preparado de anticorpos policlonais (PAP) e/ou de monensina sódica (MON) na morfologia das papilas ruminais, incidência de ruminites (IR) e abscessos hepáticos (AH) em bovinos jovens confinados. Foram utilizados 72 novilhos da raça Brangus, não castrados, com peso vivo inicial de 261,04 ± 34,73 kg, em 24 baias (3 animais/baia) com 6 repetições por tratamento. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 2 x 2, sendo os fatores a adição ou não de MON e PAP. Após o abate foram realizadas as avaliações. No fígado tanto o percentual de AH quanto a severidade foram menores (P < 0,01) nos animais que receberam PAP em relação aos que não receberam. A inclusão de MON foi apenas eficiente em reduzir (P < 0,01) a severidade dos AH em relação ao tratamento sem o aditivo. Não houve efeito dos aditivos sobre o IR. O tratamento com MON resultou em maior (P=0,07) superfície de absorção/cm2 de parede de rúmen e maior ( P=0,10) área papilar em relação ao tratamento sem este aditivo. Número de papilas/cm2 de parede e área média das papilas não apresentaram diferenças entre tratamentos. O uso de PAP foi eficiente na redução de AH bem como a severidade, porém não foi capaz de melhorar a forma e a estrutura das papilas ruminais, como o observado com o uso de MON.(AU)

The aim of this study was to evaluate the effects of polyclonal antibodies preparation (PAP) and/or monensin (MON) on the morphology of the rumen papillae, incidence of ruminate (IR) and liver abscess in feedlot bullocks. Seventy-two bullocks Brangus (261.04 ± 34.73kg) were housed in 24 pens (3 bullocks/pen) with6 replications per treatment. The experimental design was completely randomized 2 x 2 factorial, the factors werethe inclusion or not of MON and PAP.After slaughter, the evaluations were performed. For the livers, both the percentage of abscesses and the severity of them were lower (P< 0.01) in animals fed diets with PAP than those fed MON. The inclusion of MON in the diet was only effective in reducing (P< 0.01) the severity of the abscesses in relation to treatment without this additive. There was no effect (P> 0.05) of additives on the IR. The treatment with MON had higher (P = 0.07) surface absorption/cm2 wall of the rumen and greater (P = 0.10) papillary area compared to treatment without this additive. Number of papillae/cm2 wall and average area of the papillae showed no difference among treatments. The use of PAP was effective in reducing HA as well as the severity the same, but was not able to improve the shape and structure of the ruminal papillae, as observed with the use of MON.(AU)

Incidência de ruminite e abscesso hepático em bovinos jovens confinados alimentados com dietas contendo aditivos alimentares

p>The aim of this study was to evaluate the effects of polyclonal antibodies preparation (PAP) and/or monensin (MON) on the morphology of the rumen papillae, incidence of ruminate (IR) and liver abscess in feedlot bullocks. Seventy-two bullocks Brangus (261.04 ± 34.73kg) were housed in 24 pens (3 bullocks/pen) with6 replications per treatment. The experimental design was completely randomized 2 x 2 factorial, the factors werethe inclusion or not of MON and PAP.After slaughter, the evaluations were performed. For the livers, both the percentage of abscesses and the severity of them were lower ( italic>P /italic> 0.01) in animals fed diets with PAP than those fed MON. The inclusion of MON in the diet was only effective in reducing ( italic>P /italic> 0.01) the severity of the abscesses in relation to treatment without this additive. There was no effect ( italic>P /italic>> 0.05) of additives on the IR. The treatment with MON had higher ( italic>P /italic> = 0.07) surface absorption/cm sup>2 /sup> wall of the rumen and greater ( italic>P /italic> = 0.10) papillary area compared to treatment without this additive. Number of papillae/cm sup>2 /sup> wall and average area of the papillae showed no difference among treatments. The use of PAP was effective in reducing HA as well as the severity the same, but was not able to improve the shape and structure of the ruminal papillae, as observed with the use of MON. /p>

p>O objetivo deste estudo foi avaliar o efeito da inclusão do preparado de anticorpos policlonais (PAP) e/ou de monensina sódica (MON) na morfologia das papilas ruminais, incidência de ruminites (IR) e abscessos hepáticos (AH) em bovinos jovens confinados. Foram utilizados 72 novilhos da raça Brangus, não castrados, com peso vivo inicial de 261,04 ± 34,73 kg, em 24 baias (3 animais/baia) com 6 repetições por tratamento. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 2 x 2, sendo os fatores a adição ou não de MON e PAP. Após o abate foram realizadas as avaliações. No fígado tanto o percentual de AH quanto a severidade foram menores (P 0,01) nos animais que receberam PAP em relação aos que não receberam. A inclusão de MON foi apenas eficiente em reduzir (P 0,01) a severidade dos AH em relação ao tratamento sem o aditivo. Não houve efeito dos aditivos sobre o IR. O tratamento com MON resultou em maior (P=0,07) superfície de absorção/cm sup>2 /sup> de parede de rúmen e maior (P=0,10) área papilar em relação ao tratamento sem este aditivo. Número de papilas/cm sup>2 /sup> de parede e área média das papilas não apresentaram diferenças entre tratamentos. O uso de PAP foi eficiente na redução de AH bem como a severidade, porém não foi capaz de melhorar a forma e a estrutura das papilas ruminais, como o observado com o uso de MON. /p>

Avaliação do custo do ganho do kilo de peso vivo em protocolos de adaptação com dietas de alto teor concentrado para bovinos Nelore em confinamento / Evaluation of live weight cost in adaptation protocols with high concentrate diet for Nellore cattle feedlot / Evaluación del costo de la adaptación del peso kilo en vivo en protocolos con dietas altas en concentrados para el ganado en Nellore confinado

O objetivo deste estudo foi avaliar o custo do ganho de peso vivo na duração de protocolos de adaptação à dieta de alto teor de concentrado de bovinos nelore terminados em confinamento. Cento e vinte bovinos, machos não castrados da raça nelore provenientes de sistema de recria a pasto, foram mantidos em 24 baias de piso de concreto com cinco animais por baia, onde receberam alimentação de acordo com os tratamentos: T1 - adaptação em escadas (Step up) por 14 dias (S14), T2 ­ adaptação em escadas (Step up) por 21 dias (S21), T3 - protocolo de restrição por 14 dias (R14), e T4 ­ protocolo de restrição por 21 dias (R21). A adaptação em escada (step-up) consistiu no fornecimento de dietas com níveis crescentes de concentrado de acordo com o tempo (dias) de adaptação até atingir o nível de concentrado desejado para a dieta de terminação. No protocolo de restrição utilizou-se a dieta de terminação limitada por quantidade e aumentou-se o oferecimento diário gradativamente até atingir o consumo total de terminação. Após os períodos de adaptação os animais receberam uma única dieta de terminação com 85% de concentrado até o abate. Não houve diferença estatística no ganho de peso entre o período de duração dos protocolos de adaptação (14 e 21 dias), ocorrendo diferença estatística apenas nos protocolos de adaptação (escada e restrição). O protocolo de restrição obteve melhores resultados com relação ao protocolo de escada, sendo mais viável financeiramente. Ressalta-se que a adaptação dos animais por 14 dias com o protocolo de restrição, torna-se a forma menos onerosa de ganho de peso dos animais no confinamento. Salienta-se que o procedimento simples de adaptação, possibilita incremento ao confinamento de bovinos e ao agronegócio brasileiro.

This study aims to evaluate the cost of weight gain in the duration of high concentrate diet protocols for Nellore cattle feedlot. One hundred and twenty male, non-castrated Nellore cattle from grazing system were kept in 24 concrete floor pens with five animals in each one and were fed according the treatments: T1 - adaptation on stairs (Set up) for 14 days (S14); T2 - adaptation on stairs (Set up) for 21 days (S21); T3 - restriction protocol for 14 days (R14) and T4 restriction protocol for 21 days (R21). Stair adaptation (Set up) consists of supplying diets with growing levels of concentrate according to adaptation time (days) until reaching the aimed concentration for termination diets. For restriction protocols, limited termination diet are used by quantity and daily offering is gradually increased until reaching total termination consumption. After adaptation period the animals receive only one termination diet with 85% of concentrate until slaughtering. There was no statistical difference in weight gain between adaptation protocol duration (14 and 21 days). However there was statistical difference only for adaptation protocols for (stair and restriction). Restriction protocols showed better results in relation to stair protocols as well as a much better financial viability. It is noteworthy that animal adaptation for 14 days under restriction protocol makes it the least expensive way of weight gain. It is interesting to point out that the simple adaptation procedure enables increasing to cattle feedlot and to agribusiness.

El objetivo de este estudio fue evaluar el coste del aumento de peso en la duración de los protocolos para la adaptación de dieta de concentrado alto de bovino Nelore de engorde confinado. Ciento veinte bovinos macho enteros de raza Nelore, de sistema de pastoreo, se mantuvieron en 24 corrales con piso de concreto con cinco animales por corral y alimentados de acuerdo a los tratamientos: T1 - adaptación en escaleras (Step up) durante 14 días (S14), T2 - adaptación en las escaleras (Step Up) durante 21 días (S21), T3 - Protocolo de restricción durante 14 días (R14), y T4 - protocolo de restricción durante 21 días (R21). La adaptación en escalera (Step up) es la disposición de las dietas con niveles crecientes de concentrado de acuerdo con el tiempo (días) de adaptación a alcanzar el nivel de concentrado deseado para la dieta de terminación. En el protocolo de restricción se utilizó la dieta de terminación limitada por cantidad y se aumentó la oferta diaria gradualmente hasta alcanzar el consumo total de terminación. Después del periodo de adaptación, los animales recibieron una única dieta de terminación con 85% de concentrado hasta masacre. No hubo diferencia estadística en la ganancia de peso entre la duración de los protocolos de adaptación (14 y día 21), sólo diferencia estadística en los protocolos de adaptación (escalera y restricción). El protocolo de restricción se obtuvo mejores resultados con respecto al protocolo de escalera, siendo más económicamente viable. Es de destacar que la adaptación de los animales durante 14 días con el protocolo de restricción, se convierte en la forma menos costosa de ganar peso. Hay que tener en cuenta que el simple procedimiento de adaptación, permite el crecimiento del ganado confinado y el agronegócio brasilero.